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Catalase Test Protocol

  • Author: Karen Reiner1
  • VIEW AFFILIATIONS HIDE AFFILIATIONS
    Affiliations: 1: Andrews University, Berrien Springs, MI, 49104
  • Citation: Karen Reiner. 2010. Catalase test protocol.
  • Publication Date : November 2010
  • Category: Protocol
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Figure 1

Slide catalase test results. Hydrogen peroxide was added directly to the culture on a microscope slide. A positive reaction produced by Staphylococcus aureus is indicated by bubbling; a negative reaction produced by Streptococcus pyogenes is indicated by lack of bubbling.(Karen Reiner, Andrews University, Berrien Springs, MI)

Source: November 2010
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Figure 2

A positive catalase reaction produced by Staphylococcus aureus. Using a wooden applicator stick, a small amount of organism from a well-isolated 24-hour colony was collected and placed into the test tube containing hydrogen peroxide. A positive result is indicated by bubbling. (Karen Reiner, Andrews University, Berrien Springs, MI)

Source: November 2010
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Figure 3

A negative catalase reaction produced by Streptococcus pyogenes. Using a wooden applicator stick, a small amount of organism from a well-isolated 24-hour colony was collected and placed into the test tube containing hydrogen peroxide. A negative result is indicated by lack of bubbling. (Karen Reiner, Andrews University, Berrien Springs, MI)

Source: November 2010
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Figure 4

A negative catalase reaction produced by Streptococcus pyogenes. Hydrogen peroxide was added directly to 24-hour growth on a nutrient agar slant. A negative result is indicated by lack of bubbling. (Karen Reiner, Andrews University, Berrien Springs, MI)

Source: November 2010
Download as Powerpoint
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