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Nitrate and Nitrite Reduction Test Protocols

  • Author: Rebecca Buxton 1
  • VIEW AFFILIATIONS HIDE AFFILIATIONS
    Affiliations: 1: Department of Pathology, University of Utah, Salt Lake City, UT, 84132
  • Citation: Rebecca Buxton. 2011. Nitrate and nitrite reduction test protocols.
  • Publication Date : November 2011
  • Category: Protocol
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Figures

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Figure 1

This evidence shirt illustrates the presence of nitrites with a positive test for gun shot residue. The chemistry involved is the same used in the microbiology laboratory to detect nitrate (NO3) reduction to nitrite (NO2). (J. Scott Doyle, Jefferson Regional Forensic Lab, Louisville, KY)

Source: November 2011
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Figure 2

This positive Greiss test illustrates the presence of nitrites. The chemistry involved is the same used in the microbiology laboratory to detect nitrate (NO3) reduction to nitrite (NO2). (J. Scott Doyle, Jefferson Regional Forensic Lab, Louisville, KY)

Source: November 2011
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Figure 3

Uninoculated tube illustrating the proper filling of nitrate and nitrite broth. (Rebecca Buxton, University of Utah, Salt Lake City, UT)

Source: November 2011
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Figure 4

Nitrate reagents A and B. (Rebecca Buxton, University of Utah, Salt Lake City, UT)

Source: November 2011
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Figure 5

Zinc dust. (Rebecca Buxton, University of Utah, Salt Lake City, UT)

Source: November 2011
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Figure 6

"Dirtying" a stick with zinc dust. (Rebecca Buxton, University of Utah, Salt Lake City, UT)

Source: November 2011
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Figure 7

Addition of zinc dust to a nitrate test to force the reduction of the substrate. (Rebecca Buxton, University of Utah, Salt Lake City, UT)

Source: November 2011
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Figure 8

Negative nitrate reaction after the addition of reagents A and B. (Rebecca Buxton, University of Utah, Salt Lake City, UT)

Source: November 2011
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Figure 9

Negative nitrate reaction after the addition of reagents A and B and zinc dust. (Rebecca Buxton, University of Utah, Salt Lake City, UT)

Source: November 2011
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Figure 10

Negative nitrite reaction after the addition of reagents A and B. (Rebecca Buxton, University of Utah, Salt Lake City, UT)

Source: November 2011
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Figure 11

Pseudomonas aeruginosa growing in nitrate (tube labeled NO3) and nitrite (tube labeled NO2) broths before the addition of reagents, illustrating gas production. (Labeled view) (Rebecca Buxton, University of Utah, Salt Lake City, UT)

Source: November 2011
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Figure 12

Pseudomonas aeruginosa growing in nitrate (tube labeled NO3) and nitrite (tube labeled NO2) broths after the addition of reagents A and B. (Labeled view) (Rebecca Buxton, University of Utah, Salt Lake City, UT)

Source: November 2011
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Figure 13

Pseudomonas aeruginosa growing in nitrate (tube labeled NO3) and nitrite (tube labled NO2) broths after the addition of reagents A and B and zinc dust. (Labeled view) (Rebecca Buxton, University of Utah, Salt Lake City, UT)

Source: November 2011
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Figure 14

Moraxella catarrhalis growing in nitrate (tube labeled NO3) and nitrite (tube labeled NO2) broths before the addition of reagents. (Labeled view) (Rebecca Buxton, University of Utah, Salt Lake City, UT)

Source: November 2011
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Figure 15

Moraxella catarrhalis growing in nitrate (tube labled NO3) and nitrite (tube labeled NO2) broths 24 hours after the addition of reagents A and B. (Labeled view) (Rebecca Buxton, University of Utah, Salt Lake City, UT)

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Source: November 2011
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Figure 16

Moraxella catarrhalis growing in nitrate (tube labeled NO3) and nitrite (tube labeled NO2) broths 24 hours after the addition of reagents A and B and zinc dust, showing partial reduction of nitrate. (Labeled view) (Rebecca Buxton, University of Utah, Salt Lake City, UT)

Source: November 2011
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Figure 17

Moraxella catarrhalis growing in nitrate (tube labeled NO3) and nitrite (tube labeled NO2) broths 48 hours after the addition of reagents A and B and zinc dust, showing complete reduction of nitrate. (Labeled view) (Rebecca Buxton, University of Utah, Salt Lake City, UT)

Source: November 2011
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Figure 18

Escherichia coli growing in nitrate (tube labeled NO3) and nitrite (tube labeled NO2) broths before the addition of reagents. (Labeled view) (Rebecca Buxton, University of Utah, Salt Lake City, UT)

Source: November 2011
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Figure 19

Escherichia coli growing in nitrate (tube labeled NO3) and nitrite (tube labeled NO2) broths after the addition of reagents A and B. (Labeled view) (Rebecca Buxton, University of Utah, Salt Lake City, UT)

Source: November 2011
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Figure 20

Neisseria lactamica growing in nitrate (tube labeled NO3) and nitrite (tube labeled NO2) broths before the addition of reagents. (Labeled view) (Rebecca Buxton, University of Utah, Salt Lake City, UT)

Source: November 2011
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Figure 21

Neisseria lactamica growing in nitrate (tube labeled NO3) and nitrite (tube labeled NO2) after the addition of reagents A and B. (Labeled view) (RebeccaBuxton, University of Utah, Salt Lake City, UT)

Source: November 2011
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Figure 22

Neisseria lactamica growing innitrate (tube labeled NO3) and nitrite (tube labeled NO2) broths after the addition of reagents A and B and zinc dust. (Labeled view) (Rebecca Buxton, University of Utah, Salt Lake City, UT)

Source: November 2011
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