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Gelatin Hydrolysis Test Protocol

  • Authors: Thomas Edison E. dela Cruz1, Jeremy Martin O. Torres2
  • VIEW AFFILIATIONS HIDE AFFILIATIONS
    Affiliations: 1: University of Santo Tomas, Sampaloc, Manila; 2: Ateneo de Manila University, Loyola Heights, Quezon City
  • Citation: Thomas Edison E. dela Cruz, Jeremy Martin O. Torres. 2012. Gelatin hydrolysis test protocol.
  • Publication Date : November 2012
  • Category: Protocol
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Figures

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Figure 1

Gelatin hydrolysis test. After 30 minutes in an ice bath, the nutrient gelatin tube inoculated with Bacillus subtilis exhibited positive gelatin hydrolysis as shown by medium liquefaction. Gelatin hydrolysis was observed after 3 days of incubation. (Thomas Edison dela Cruz and Jeremy Martin O. Torres, University of Santo Tomas, Manila, Philippines)

Source: November 2012
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Figure 2

Gelatin hydrolysis test. After 30 minutes in an ice bath, the uninoculated control remained solid. (Thomas Edison dela Cruz and Jeremy Martin O. Torres, University of Santo Tomas, Manila, Philippines)

Source: November 2012
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Figure 3

Gelatin hydrolysis test using the nutrient gelatin plate method. Positive gelatin hydrolysis exhibited by Bacillus subtilis (A) is indicated by the clear zone around the colony after the addition of saturated ammonium sulfate. Negative gelatin hydrolysis exhibited by Escherichia coli (B) is indicated by the absence of a clearing zone around the colony. Inoculated culture plates were incubated for 24 hours. (Anne Hanson, University of Maine, Orono, ME)

Source: November 2012
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