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Carbohydrate Fermentation Protocol

  • Author: Karen Reiner1
  • VIEW AFFILIATIONS HIDE AFFILIATIONS
    Affiliations: 1: Andrews University, Berrien Springs, MI, 49104
  • Citation: Karen Reiner. 2012. Carbohydrate fermentation protocol.
  • Publication Date : November 2012
  • Category: Protocol
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Figures

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Figure 1

Peptone media with phenol red indicator. From left to right: uninoculated tube, glucose fermenter (Escherichia coli) with gas production (visible carbon dioxide bubble in the inverted Durham tube), glucose fermenter (Shigella sonnei) without gas production (no visible carbon dioxide bubble in the inverted Durham tube), nonfermenter (Pseudomonas aeruginosa). Pseudomonas aeruginosa does not ferment sugars leaving the red pH-indicating chemical in the medium unchanged from the control. Escherichia coli and Shigella sonnei are members of the Enterobacteriaceae family and ferment glucose to acid, producing a change in the pH-indicating chemical in the media. (Karen Reiner, Andrews University, Berrien Springs, MI)

Source: November 2012
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Image of Figure 2

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Figure 2

Peptone media with bromocresol purple indicator. From left to right: uninoculated tube, glucose fermenter (Escherichia coli) with gas production (visible carbon dioxide bubble in the inverted Durham tube), glucose fermenter (Shigella sonnei) without gas production (no visible carbon dioxide bubble in the inverted Durham tube), nonfermenter (Pseudomonas aeruginosa). Pseudomonas aeruginosa does not ferment sugars leaving the red pH-indicating chemical in the medium unchanged from the control. Escherichia coli and Shigella sonnei are members of the Enterobacteriaceae family and ferment glucose to acid, producing a change in the pH-indicating chemical in the media. (Karen Reiner, Andrews University, Berrien Springs, MI)

Source: November 2012
Download as Powerpoint
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