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Decarboxylase Broth Protocol

  • Authors: Archana Lal1, Naowarat Cheeptham 2
  • VIEW AFFILIATIONS HIDE AFFILIATIONS
    Affiliations: 1: Independence Community College, Independence, KS, 67301; 2: Thompson Rivers University, Kamloops, BC V2C 5N3
  • Citation: Archana Lal, Naowarat Cheeptham. 2015. Decarboxylase broth protocol.
  • Publication Date : September 2015
  • Category: Protocol
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Figures

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Figure 1

Moeller's Decarboxylase media: A: Uninoculated base, B: Base inoculated with E. coli, C: Arginine broth inoculated with E. coli, D: Lysine broth inoculated with E. coli, E: Ornithine broth inoculated with E. coli. Purple color in tubes C, D, and E indicate that this strain of E. coli is arginine, lysine, and ornithine decarboxylase positive. (Archana Lal, Independence Community College, Independence, KS)

Source: September 2015
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Figure 2

Moeller's Decarboxylase media: A: Uninoculated base, B: Base inoculated with Enterobacter cloacae, C: Arginine broth inoculated with E. cloacae, D: Lysine broth inoculated with E. cloacae, E: Ornithine broth inoculated with E. cloacae. Purple color in tubes C and E indicate that E. cloacae is arginine and ornithine decarboxylase positive while yellow color in tube D (absence of purple color) indicates that it is lysine decarboxylase negative. (Archana Lal, Independence Community College, Independence, KS)

Source: September 2015
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Figure 3

Moeller's Decarboxylase media: A: Uninoculated base, B: Base inoculated with Klebsiella oxytoca, C: Arginine broth inoculated with K. oxytoca, D: Lysine broth inoculated with K. oxytoca, E: Ornithine broth inoculated with K. oxytoca. Purple color in tube D indicates that K. oxytoca is lysine decarboxylase positive while yellow color in tubes C and E (absence of purple color) indicates that it is arginine and ornithine decarboxylase negative. (Archana Lal, Independence Community College, Independence, KS)

Source: September 2015
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Figure 4

Moeller's Decarboxylase media: A: Uninoculated base, B: Base inoculated with Klebsiella pneumoniae, C: Arginine broth inoculated with K. pneumoniae, D: Lysine

broth inoculated with K. pneumoniae, E: Ornithine broth inoculated with K. pneumoniae. Purple color in tube D indicates that K. pneumoniae is lysine decarboxylase positive while yellow color in tubes C and E (absence of purple color) indicates that it is arginine and ornithine decarboxylase negative. (Archana Lal, Independence Community College, Independence, KS)

Source: September 2015
Download as Powerpoint
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