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Perfecting Your Spread Plate Technique

    Author: Diane Hartman1
    VIEW AFFILIATIONS HIDE AFFILIATIONS
    Affiliations: 1: Department of Biology, Baylor University, Waco, TX 76798
    AUTHOR AND ARTICLE INFORMATION AUTHOR AND ARTICLE INFORMATION
    • Published 01 December 2011
    • Corresponding author. Mailing address: Baylor University, One Bear Place #97388, Waco, TX 76798. Phone: (254) 710-2107. Fax: (254)710-2969. E-mail: Diane_Hartman@baylor.edu.
    • Copyright © 2011 American Society for Microbiology
    Source: J. Microbiol. Biol. Educ. December 2011 vol. 12 no. 2 204-205. doi:10.1128/jmbe.v12i2.324
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    Abstract:

    The Spread Plate Technique, in conjunction with serial dilutions, is a valuable research tool. In this example, dye is used to accentuate the visible volume of each aliquot and the dispersion pattern on the agar. With the utilization of a second dye, each agar plate can be used twice. The immediate feedback and personal evaluation of these techniques provide an active learning experience that students remember.

Key Concept Ranking

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2011-12-01
2017-09-20

Abstract:

The Spread Plate Technique, in conjunction with serial dilutions, is a valuable research tool. In this example, dye is used to accentuate the visible volume of each aliquot and the dispersion pattern on the agar. With the utilization of a second dye, each agar plate can be used twice. The immediate feedback and personal evaluation of these techniques provide an active learning experience that students remember.

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