1887

A New Twist to the Kirby-Bauer Antibiotic Susceptibility Test Activity—Increasing Antibiotic Sensitivity of through Thermal Stress

    Authors: Donald G. Gerbig Jr.1,*, Jean Engohang-Ndong1, Heather Aubihl1
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    Affiliations: 1: Department of Biological Sciences, Kent State University at Tuscarawas, New Philadelphia, OH 44663
    AUTHOR AND ARTICLE INFORMATION AUTHOR AND ARTICLE INFORMATION
    • Published 02 December 2013
    • *Corresponding author. Mailing address: Department of Biological Sciences – Kent State University at Tuscarawas, 330 University Dr. NE; New Philadelphia, OH 44663. Phone: 330-308-7484. Fax: 330-339-3321. E-mail: dgerbig@kent.edu.
    • ©2013 Author(s). Published by the American Society for Microbiology.
    Source: J. Microbiol. Biol. Educ. December 2013 vol. 14 no. 2 269-270. doi:10.1128/jmbe.v14i2.617
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    Abstract:

    Antibiotic sensitivity and the effect of temperature on microbial growth are two standard laboratory activities found in most microbial laboratory manuals. We have found a novel way to combine the two activities to demonstrate how temperature can influence antibiotic sensitivity using a standard incubator in instructional laboratory settings. This activity reinforces the important concepts of microbial growth and temperature along with Kirby-Bauer antibiotic susceptibility testing. We found that Pseudomonas fluorescens can be manipulated to become more sensitive to several antibiotics by simply increasing growth temperature and exposing the organism to various antibiotics. No additional equipment is required beyond a standard incubator. Pseudomonas fluorescens is an excellent choice for this activity since it is a safe alternative to Pseudomonas aeruginosa, a biosafety level 2 agent. Pseudomonads are important to explore in the microbiology laboratory since Pseudomonas aeruginosa poses a serious issue in health care settings, as this organism is known to be a multi-drug-resistant pathogen (6). More importantly, P. fluorescens is a good alternative in the laboratory to P. aeruginosa since it is also pigmented (5) and a possible reservoir of antibiotic resistance genes (4). In addition, it grows best at room temperatures and can easily be thermally stressed by placing in a standard 35ºC to 37ºC incubator

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Pseudomonas fluorescens
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References & Citations

1. Adebusuyi AA, Foght JM 2011 An alternative physiological role for the EmhABC efflux pump in Pseudomonas fluorescens cLP6a BMC Microbiol 11 252 [Online.] http://www.biomedcentral.com/1471-2180/11/252 10.1186/1471-2180-11-252 http://dx.doi.org/10.1186/1471-2180-11-252
2. Burger M, RG Woods RG, McCarthy C, Beacham IR 2000 Temperature regulation of protease in Pseudomonas fluorescens LS107d2 by an ECF sigma factor and a transmembrane activator Microbiol 146 3149 3155
3. Hudzicki J 8 December 2009 posting date Kirby-Bauer disk diffusion susceptibility test protocol American Society for Microbiology Washington, DC [Online.] http://www.microbelibrary.org/component/resource/laboratory-test/3189-kirby-bauer-disk-diffusion-susceptibility-test-protocol
4. Maravic A, Skocibusic M, Samanic I, Puizina J 2012 Antibiotic susceptibility profiles and first report of TEM extended-spectrum beta-lactamase in Pseudomonas fluorescens from coastal waters of Kastela Bay, Croatia World J Microbiol Biotechnol 28 2039 2045 10.1007/s11274-012-1006-5 http://dx.doi.org/10.1007/s11274-012-1006-5
5. Meyer JM, Abdallah MA 1978 The fluorescent pigment of Pseudomonas fluorescens: biosynthesis, purification, and physicochemical properties J Gen Microboiol 107 319 328 10.1099/00221287-107-2-319 http://dx.doi.org/10.1099/00221287-107-2-319
6. Pool K 2011 Pseudomonas aeruginosa: resistance to the max Front. in Microbiol 2 65 [Online.] http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3128976/?report=classic
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/content/journal/jmbe/10.1128/jmbe.v14i2.617
2013-12-02
2017-11-18

Abstract:

Antibiotic sensitivity and the effect of temperature on microbial growth are two standard laboratory activities found in most microbial laboratory manuals. We have found a novel way to combine the two activities to demonstrate how temperature can influence antibiotic sensitivity using a standard incubator in instructional laboratory settings. This activity reinforces the important concepts of microbial growth and temperature along with Kirby-Bauer antibiotic susceptibility testing. We found that Pseudomonas fluorescens can be manipulated to become more sensitive to several antibiotics by simply increasing growth temperature and exposing the organism to various antibiotics. No additional equipment is required beyond a standard incubator. Pseudomonas fluorescens is an excellent choice for this activity since it is a safe alternative to Pseudomonas aeruginosa, a biosafety level 2 agent. Pseudomonads are important to explore in the microbiology laboratory since Pseudomonas aeruginosa poses a serious issue in health care settings, as this organism is known to be a multi-drug-resistant pathogen (6). More importantly, P. fluorescens is a good alternative in the laboratory to P. aeruginosa since it is also pigmented (5) and a possible reservoir of antibiotic resistance genes (4). In addition, it grows best at room temperatures and can easily be thermally stressed by placing in a standard 35ºC to 37ºC incubator

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