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A Publicly Available PCR Methods Laboratory Manual and Supporting Material

    Authors: Jason DeCaire1, Cappi Ker1, William Johnston1, Mary Muslow1, Jeffry Shultz1,*
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    Affiliations: 1: School of Biological Sciences, Louisiana Tech University, Ruston, LA 71272
    AUTHOR AND ARTICLE INFORMATION AUTHOR AND ARTICLE INFORMATION
    • Supplemental materials available at http://jmbe.asm.org
    • *Corresponding author. Mailing address: School of Biological Sciences, Louisiana Tech University, 1 Adams Boulevard, Ruston, LA 71272. Phone: 318-257-2753. Fax 318-257-4574. E-mail: jlshultz@latech.edu.
    • ©2015 Author(s). Published by the American Society for Microbiology.
    Source: J. Microbiol. Biol. Educ. December 2015 vol. 16 no. 2 269-270. doi:10.1128/jmbe.v16i2.887
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    Abstract:

    We report the creation of a PCR methods laboratory manual and supporting material. The manual contains 24 different procedures designed to illustrate basic PCR concepts and promote independent problem-solving skills. Each lab poses a question about PCR and provides a list of materials the student may use to investigate the question. Supplemental materials available to instructors include answers to post-lab questions, lab set-up information, 82 assessment questions and answers, 22 PowerPoint slides, a sample syllabus, experiment set-up illustrations and example results for most labs. The student lab book is available as a supplemental file and at http://ans.latech.edu/BasicPCRMethods.html, while Instructor-specific materials are available from the corresponding author at no charge.

References & Citations

1. Degen HJ, Deufel A, Eisel D, Grunewald-Janho S, Keesey J2006PCR applications manualRoche, Mannheim, Germany
2. McPherson MJ, Moller SG2006PCR (the basics)2nd edTaylor and FrancisNew York, NY
3. Nolan T, Bustin SA2013PCR technology: current innovations3rd edCRC PressBoca Raton, FL10.1201/b14930 http://dx.doi.org/10.1201/b14930
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/content/journal/jmbe/10.1128/jmbe.v16i2.887
2015-12-01
2017-08-20

Abstract:

We report the creation of a PCR methods laboratory manual and supporting material. The manual contains 24 different procedures designed to illustrate basic PCR concepts and promote independent problem-solving skills. Each lab poses a question about PCR and provides a list of materials the student may use to investigate the question. Supplemental materials available to instructors include answers to post-lab questions, lab set-up information, 82 assessment questions and answers, 22 PowerPoint slides, a sample syllabus, experiment set-up illustrations and example results for most labs. The student lab book is available as a supplemental file and at http://ans.latech.edu/BasicPCRMethods.html, while Instructor-specific materials are available from the corresponding author at no charge.

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FIGURE 1

Example of master mix setup diagram. Note that this is provided to students as an example—subsequent master mix diagrams are only provided in the instructor materials. Inset shows student generated gel picture of lab.

Source: J. Microbiol. Biol. Educ. December 2015 vol. 16 no. 2 269-270. doi:10.1128/jmbe.v16i2.887
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