1887

Identification of an Alternative to Proteus vulgaris as a Laboratory Standard for Hydrogen Sulfide Production

    Authors: Nar’Asha Randall1, Cara Nance1, Cathy Huggins1, James Huggins1, Mark Bolyard1,*
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    Affiliations: 1: Department of Biology, Union University, Jackson, TN 38305
    AUTHOR AND ARTICLE INFORMATION AUTHOR AND ARTICLE INFORMATION
    • Received 08 August 2016 Accepted 09 November 2016 Published 21 April 2017
    • ©2017 Author(s). Published by the American Society for Microbiology.
    • [open-access] This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial-NoDerivatives 4.0 International license (https://creativecommons.org/licenses/by-nc-nd/4.0/ and https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode), which grants the public the nonexclusive right to copy, distribute, or display the published work.

    • *Corresponding author. Mailing address: Biology Department, Union University, 1050 Union University Drive, Box 3021, Jackson, TN 38305. Phone: 731-661-6586. E-mail: mbolyard@uu.edu.
    Source: J. Microbiol. Biol. Educ. April 2017 vol. 18 no. 1 doi:10.1128/jmbe.v18i1.1219
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    Abstract:

    This project involved the evaluation of a biosafety level 1 alternative to Proteus vulgaris as a positive control for the production of hydrogen sulfide. We determined that Citrobacter freundii could serve as an excellent substitute for P. vulgaris, and that lead acetate strips used in conjunction with triple sugar iron media allows for consistent results following evaluation after up to one week.

Key Concept Ranking

Hydrogen Sulfide
0.6346154
Proteus vulgaris
0.5815069
0.6346154

References & Citations

1. Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST2000Bergey’s manual of determinative bacteriologyLippincot, Williams & WilkinsPhiladelphia, PA
2. Swamy PM2008Laboratory manual on biotechnologyRastogi PublicationsMeerut – New Delhi, India
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/content/journal/jmbe/10.1128/jmbe.v18i1.1219
2017-04-21
2017-11-23

Abstract:

This project involved the evaluation of a biosafety level 1 alternative to Proteus vulgaris as a positive control for the production of hydrogen sulfide. We determined that Citrobacter freundii could serve as an excellent substitute for P. vulgaris, and that lead acetate strips used in conjunction with triple sugar iron media allows for consistent results following evaluation after up to one week.

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FIGURE 1

Lead acetate strips from , , and from a 48-hour stab culture using triple sugar iron (TSI) or peptone iron (PI).

Source: J. Microbiol. Biol. Educ. April 2017 vol. 18 no. 1 doi:10.1128/jmbe.v18i1.1219
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