1887

siRNA Immunological Fishing Training (SIFT) Experience as a Novel Research Education Tool for Students Studying Immunology

    Authors: Diana M. Elizondo1, Temesgen E. Andargie1, Dineeta S. Kubhar1, Karis M. Marshall1, Aamir M. Zariwala1, Clarence M. Lee1, Michael W. Lipscomb1,*
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    Affiliations: 1: Department of Biology, Howard University, Washington, DC 20059
    AUTHOR AND ARTICLE INFORMATION AUTHOR AND ARTICLE INFORMATION
    Source: J. Microbiol. Biol. Educ. April 2017 vol. 18 no. 1 doi:10.1128/jmbe.v18i1.1288
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    Abstract:

    This report describes an innovative research education tool for student exposure and training in immunology. Utilizing the wealth of transcriptomics data provided through the immunological genome project, graduate and undergraduate students can engage in hands-on experimental studies to identify and rigorously characterize novel genes in immune cells using RNA interference-mediated approaches. The SIFT activities are directly aligned with cell biology and immunology courses and serve to reinforce and expand on the foundations learned. Furthermore, the student-driven studies provide a sense of ownership and largely expose students to the breadth of experimental research approaches in the biological sciences. Finally, generation of original datasets supports the greater scientific community by contributing in assembly and delineation of the regulatory networks that govern immunity.

Key Concept Ranking

Immune Cells
0.6497925
B Cells
0.5248334
Immune Response
0.49406302
Western Blotting
0.46519327
0.6497925

References & Citations

1. Woodin T, Carter VC, Fletcher L2010Vision and change in biology undergraduate education, a call for action—initial responsesCBE Life Sci Educ9717310.1187/cbe.10-03-0044205163502879380 http://dx.doi.org/10.1187/cbe.10-03-0044
2. Kuldell NH2006How golden is silence? Teaching undergraduates the power and limits of RNA interferenceCBE Life Sci Educ524725410.1187/cbe.05-11-0124170122161618687 http://dx.doi.org/10.1187/cbe.05-11-0124
3. Heng TS, Painter MW2008The immunological genome project: networks of gene expression in immune cellsNat Immunol91091109410.1038/ni1008-109118800157 http://dx.doi.org/10.1038/ni1008-1091
4. Miller JC, Brown BD, Shay T, Gautier EL, Jojic V, Cohain A, Pandey G, Leboeuf M, Elpek KG, Helft J, Hashimoto D, Chow A, Price J, Greter M, Bogunovic M, Bellemare-Pelletier A, Frenette PS, Randolph GJ, Turley SJ, Merad M2012Deciphering the transcriptional network of the dendritic cell lineageNature Immunol1388889910.1038/ni.2370 http://dx.doi.org/10.1038/ni.2370
5. Shay T, Kang J2013Immunological genome project and systems immunologyTrends Immunol3460260910.1016/j.it.2013.03.004236319364615706 http://dx.doi.org/10.1016/j.it.2013.03.004
6. Elizondo DM, Andargie TE, Marshall KM, Zariwala AM, Lipscomb MW2016Dendritic cell expression of ADAM23 governs T cell proliferation and cytokine production through the alpha(v)beta(3) integrin receptorJ Leukocyte Biol10085586410.1189/jlb.2HI1115-525R http://dx.doi.org/10.1189/jlb.2HI1115-525R
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2017-04-21
2017-11-22

Abstract:

This report describes an innovative research education tool for student exposure and training in immunology. Utilizing the wealth of transcriptomics data provided through the immunological genome project, graduate and undergraduate students can engage in hands-on experimental studies to identify and rigorously characterize novel genes in immune cells using RNA interference-mediated approaches. The SIFT activities are directly aligned with cell biology and immunology courses and serve to reinforce and expand on the foundations learned. Furthermore, the student-driven studies provide a sense of ownership and largely expose students to the breadth of experimental research approaches in the biological sciences. Finally, generation of original datasets supports the greater scientific community by contributing in assembly and delineation of the regulatory networks that govern immunity.

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