1887

Demonstrating Principles of Metabolism through Herbicide Treatment of Microalgae

    Author: Betsy Leverett1
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    Affiliations: 1: Department of Chemistry and Biochemistry, University of the Incarnate Word, San Antonio, TX 78209
    AUTHOR AND ARTICLE INFORMATION AUTHOR AND ARTICLE INFORMATION
    Source: J. Microbiol. Biol. Educ. October 2017 vol. 18 no. 3 doi:10.1128/jmbe.v18i3.1326
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    Abstract:

    An undergraduate laboratory experiment is described which illustrates blockade of a metabolic pathway and the potential environmental impacts of some herbicides. Students use a spectrophotometric assay to examine branched chain amino acid (BCAA) depletion in microalgae cultures treated with sulfosulfuron, an herbicide and known inhibitor of BCAA biosynthesis. Students also compare the growth of microalgae with and without sulfosulfuron treatment. The experiment provides student training in basic cell culture, biochemical assay techniques, and the use of a microplate spectrophotometer.

Key Concept Ranking

Amino Acid Synthesis
0.484282
Freshwater Algae
0.4706
Amino Acids
0.46481505
0.484282

References & Citations

1. Wallert MA, Foster JD, Scholnick DA, Olmschenk SM, Kuehn BJ, Provost JJ 2001 Kinetic analysis of glucose-6-phosphatase: an investigative approach to carbohydrate metabolism and kinetics Biochem Molec Biol Educ 29 199 203
2. Giles BJ, Matsche Z, Egeland RD, Reed RA, Morioka SS, Taber RL 1999 An in vivo 13C NMR analysis of the anaerobic yeast metabolism of 1- 13C-glucose J Chem Educ 76 1564 1566 10.1021/ed076p1564 http://dx.doi.org/10.1021/ed076p1564
3. Razeghifard R, Chiafair CE, Giarikos DG 2014 A P450 metabolism experiment for undergraduate biochemistry laboratories J Chem Educ 91 1 141 144 10.1021/ed400425d http://dx.doi.org/10.1021/ed400425d
4. Whitcomb CE 1999 An introduction to ALS-inhibiting herbicides Toxicol Ind Health 15 231 239 10.1191/074823399678846592 10188205 http://dx.doi.org/10.1191/074823399678846592
5. Nyström B, Björnsäter B, Blanck H 1999 Effects of sulfonylurea herbicides on non-target aquatic microorganisms: growth inhibition of micro-algae Aquatic Toxicol 47 9 22 10.1016/S0166-445X(99)00007-7 http://dx.doi.org/10.1016/S0166-445X(99)00007-7
6. Fairchild JF, Ruessler DS, Haverland PS, Carlson AR 1997 Comparative sensitivity of Selenastrum capricornutum and Lemna minor to sixteen herbicides Arch Environ Contamin Toxicol 32 4 353 357 10.1007/s002449900196 http://dx.doi.org/10.1007/s002449900196
7. Postma PR, Miron TL, Olivieri G, Barbosa MJ, Wijffels RH, Eppink MH 2015 Mild disintegration of the green microalgae Chlorella vulgaris using bead milling Bioresour Technol 184 297 304 10.1016/j.biortech.2014.09.033 http://dx.doi.org/10.1016/j.biortech.2014.09.033
8. Li H, Zhu D, Hyatt BA, Malik FM, Biehl ER, Hua L 2009 Cloning, protein sequence clarification, and substrate specificity of a leucine dehydrogenase from Bacillus sphaericus ATCC4525 Appl Biochem Biotechnol 158 343 351 10.1007/s12010-008-8304-2 http://dx.doi.org/10.1007/s12010-008-8304-2

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2017-10-30
2019-05-25

Abstract:

An undergraduate laboratory experiment is described which illustrates blockade of a metabolic pathway and the potential environmental impacts of some herbicides. Students use a spectrophotometric assay to examine branched chain amino acid (BCAA) depletion in microalgae cultures treated with sulfosulfuron, an herbicide and known inhibitor of BCAA biosynthesis. Students also compare the growth of microalgae with and without sulfosulfuron treatment. The experiment provides student training in basic cell culture, biochemical assay techniques, and the use of a microplate spectrophotometer.

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FIGURE 1

The biosynthesis of isoleucine, valine, and leucine (branched-chain amino acids, BCAAs), and inhibition by sulfosulfuron.

Source: J. Microbiol. Biol. Educ. October 2017 vol. 18 no. 3 doi:10.1128/jmbe.v18i3.1326
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