1887

Demonstrating Principles of Metabolism through Herbicide Treatment of Microalgae

    Author: Betsy Leverett1
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    Affiliations: 1: Department of Chemistry and Biochemistry, University of the Incarnate Word, San Antonio, TX 78209
    AUTHOR AND ARTICLE INFORMATION AUTHOR AND ARTICLE INFORMATION
    Source: J. Microbiol. Biol. Educ. October 2017 vol. 18 no. 3 doi:10.1128/jmbe.v18i3.1326
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    Abstract:

    An undergraduate laboratory experiment is described which illustrates blockade of a metabolic pathway and the potential environmental impacts of some herbicides. Students use a spectrophotometric assay to examine branched chain amino acid (BCAA) depletion in microalgae cultures treated with sulfosulfuron, an herbicide and known inhibitor of BCAA biosynthesis. Students also compare the growth of microalgae with and without sulfosulfuron treatment. The experiment provides student training in basic cell culture, biochemical assay techniques, and the use of a microplate spectrophotometer.

Key Concept Ranking

Amino Acid Synthesis
0.484282
Freshwater Algae
0.4706
Amino Acids
0.46481505
0.484282

References & Citations

1. Wallert MA, Foster JD, Scholnick DA, Olmschenk SM, Kuehn BJ, Provost JJ2001Kinetic analysis of glucose-6-phosphatase: an investigative approach to carbohydrate metabolism and kineticsBiochem Molec Biol Educ29199203
2. Giles BJ, Matsche Z, Egeland RD, Reed RA, Morioka SS, Taber RL1999An in vivo 13C NMR analysis of the anaerobic yeast metabolism of 1-13C-glucoseJ Chem Educ761564156610.1021/ed076p1564 http://dx.doi.org/10.1021/ed076p1564
3. Razeghifard R, Chiafair CE, Giarikos DG2014A P450 metabolism experiment for undergraduate biochemistry laboratoriesJ Chem Educ91114114410.1021/ed400425d http://dx.doi.org/10.1021/ed400425d
4. Whitcomb CE1999An introduction to ALS-inhibiting herbicidesToxicol Ind Health1523123910.1191/07482339967884659210188205 http://dx.doi.org/10.1191/074823399678846592
5. Nyström B, Björnsäter B, Blanck H1999Effects of sulfonylurea herbicides on non-target aquatic microorganisms: growth inhibition of micro-algaeAquatic Toxicol4792210.1016/S0166-445X(99)00007-7 http://dx.doi.org/10.1016/S0166-445X(99)00007-7
6. Fairchild JF, Ruessler DS, Haverland PS, Carlson AR1997Comparative sensitivity of Selenastrum capricornutum and Lemna minor to sixteen herbicidesArch Environ Contamin Toxicol32435335710.1007/s002449900196 http://dx.doi.org/10.1007/s002449900196
7. Postma PR, Miron TL, Olivieri G, Barbosa MJ, Wijffels RH, Eppink MH2015Mild disintegration of the green microalgae Chlorella vulgaris using bead millingBioresour Technol18429730410.1016/j.biortech.2014.09.033 http://dx.doi.org/10.1016/j.biortech.2014.09.033
8. Li H, Zhu D, Hyatt BA, Malik FM, Biehl ER, Hua L2009Cloning, protein sequence clarification, and substrate specificity of a leucine dehydrogenase from Bacillus sphaericus ATCC4525Appl Biochem Biotechnol15834335110.1007/s12010-008-8304-2 http://dx.doi.org/10.1007/s12010-008-8304-2
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/content/journal/jmbe/10.1128/jmbe.v18i3.1326
2017-10-30
2017-11-19

Abstract:

An undergraduate laboratory experiment is described which illustrates blockade of a metabolic pathway and the potential environmental impacts of some herbicides. Students use a spectrophotometric assay to examine branched chain amino acid (BCAA) depletion in microalgae cultures treated with sulfosulfuron, an herbicide and known inhibitor of BCAA biosynthesis. Students also compare the growth of microalgae with and without sulfosulfuron treatment. The experiment provides student training in basic cell culture, biochemical assay techniques, and the use of a microplate spectrophotometer.

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FIGURE 1

The biosynthesis of isoleucine, valine, and leucine (branched-chain amino acids, BCAAs), and inhibition by sulfosulfuron.

Source: J. Microbiol. Biol. Educ. October 2017 vol. 18 no. 3 doi:10.1128/jmbe.v18i3.1326
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