1887

Demonstrating Principles of Metabolism through Herbicide Treatment of Microalgae

    Author: Betsy Leverett1
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    Affiliations: 1: Department of Chemistry and Biochemistry, University of the Incarnate Word, San Antonio, TX 78209
    AUTHOR AND ARTICLE INFORMATION AUTHOR AND ARTICLE INFORMATION
    Source: J. Microbiol. Biol. Educ. October 2017 vol. 18 no. 3 doi:10.1128/jmbe.v18i3.1326
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    Abstract:

    An undergraduate laboratory experiment is described which illustrates blockade of a metabolic pathway and the potential environmental impacts of some herbicides. Students use a spectrophotometric assay to examine branched chain amino acid (BCAA) depletion in microalgae cultures treated with sulfosulfuron, an herbicide and known inhibitor of BCAA biosynthesis. Students also compare the growth of microalgae with and without sulfosulfuron treatment. The experiment provides student training in basic cell culture, biochemical assay techniques, and the use of a microplate spectrophotometer.

Key Concept Ranking

Amino Acid Synthesis
0.484282
Freshwater Algae
0.4706
Amino Acids
0.46481505
0.484282

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/content/journal/jmbe/10.1128/jmbe.v18i3.1326
2017-10-30
2018-07-16

Abstract:

An undergraduate laboratory experiment is described which illustrates blockade of a metabolic pathway and the potential environmental impacts of some herbicides. Students use a spectrophotometric assay to examine branched chain amino acid (BCAA) depletion in microalgae cultures treated with sulfosulfuron, an herbicide and known inhibitor of BCAA biosynthesis. Students also compare the growth of microalgae with and without sulfosulfuron treatment. The experiment provides student training in basic cell culture, biochemical assay techniques, and the use of a microplate spectrophotometer.

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FIGURE 1

The biosynthesis of isoleucine, valine, and leucine (branched-chain amino acids, BCAAs), and inhibition by sulfosulfuron.

Source: J. Microbiol. Biol. Educ. October 2017 vol. 18 no. 3 doi:10.1128/jmbe.v18i3.1326
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