1887

The PARE Project: A Short Course-Based Research Project for National Surveillance of Antibiotic-Resistant Microbes in Environmental Samples

    Authors: Elizabeth A. Genné-Bacon1, Carol A. Bascom-Slack1,*
    VIEW AFFILIATIONS HIDE AFFILIATIONS
    Affiliations: 1: Center for Translational Science Education, Department of Medical Education, Tufts University School of Medicine, Boston, MA 02111
    AUTHOR AND ARTICLE INFORMATION AUTHOR AND ARTICLE INFORMATION
    • Received 13 March 2018 Accepted 18 September 2018 Published 31 October 2018
    • ©2018 Author(s). Published by the American Society for Microbiology.
    • [open-access] This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial-NoDerivatives 4.0 International license (https://creativecommons.org/licenses/by-nc-nd/4.0/ and https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode), which grants the public the nonexclusive right to copy, distribute, or display the published work.

    • Supplemental materials available at http://asmscience.org/jmbe
    • *Corresponding author. Mailing address: Center for Translational Science Education, Department of Medical Education, Tufts University School of Medicine, 136 Harrison Ave., Boston, MA 02111. Phone: 617-636-2479. Fax: 617-636-0375. E-mail: [email protected].
    Source: J. Microbiol. Biol. Educ. October 2018 vol. 19 no. 3 doi:10.1128/jmbe.v19i3.1603
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    Abstract:

    Course-based research experiences (CREs) have been proposed as an inclusive model to expose all students, including those at institutions without a strong research infrastructure, to research at an early stage. Converting an entire semester-long course can be time consuming for instructors and expensive for institutions, so we have developed a short CRE that can be implemented in a variety of life science course types. The Prevalence of Antibiotic Resistance in the Environment (PARE) project uses common microbiology methods and equipment to engage students in nationwide surveillance of environmental soil samples to document the prevalence of antibiotic-resistant bacteria. The project has been implemented at institutions ranging from community colleges to doctoral-granting institutions in 30 states plus Puerto Rico. Programmatic feedback was obtained from instructors over three iterations, and revisions were made based on this feedback. Student learning was measured by pre/post assessment in a subset of institutions. Outcomes indicate that students made significant gains in the project learning goals. Journal of Microbiology & Biology Education

References & Citations

1. Alexander B, Foertsch J, Daffinrud S 2010 The spend a summer with a scientist program: an evaluation of program outcomes and the essential elements of success University of Madison-Wisconsin, LEAD Center Madison, WI
2. Gregerman SR, Lerner JS, von Hippel W, Jonides J, Nagda BA 1998 Undergraduate student–faculty research partnerships affect student retention Rev High Educ 22 55 72 10.1353/rhe.1998.0016 http://dx.doi.org/10.1353/rhe.1998.0016
3. Kremer JF, Bringle RG 1990 The effects of an intensive research experience on the careers of talented undergraduates J Res Dev Educ 24 1 5
4. Laursen SL, Hunter A, Seymour E, Thiry H, Melton G 2010 Undergraduate research in the sciences: engaging students in real science Jossey-Bass San Francisco, CA
5. Lopatto D 2004 Survey of undergraduate research experiences (SURE): first findings Cell Biol Educ 3 270 277 10.1187/cbe.04-07-0045 15592600 533131 http://dx.doi.org/10.1187/cbe.04-07-0045
6. Russell SH, Hancock MP, McCullough J, Roessner JD, Storey C 2005 Evaluation of NSF support for undergraduate research opportunities: survey of STEM graduates: draft final report SRI International Arlington, VA
7. Zydney AL, Bennett JS, Shahid A, Bauer KW 2002 Impact of undergraduate research experience in engineering J Engineer Educ 91 151 157 10.1002/j.2168-9830.2002.tb00687.x http://dx.doi.org/10.1002/j.2168-9830.2002.tb00687.x
8. President’s Council of Advisors on Science and Technology (PCAST) 2013 Engage to excel: producing one million additional college graduates with degrees in science, technology, engineering, and mathematics Executive Office of the President Washington, DC
9. National Academies of Sciences, Engineering, and Medicine 2015 Integrating discovery-based research into the undergraduate curriculum: report of a convocation The National Academies Press Washington, DC
10. Mader CM, Beck CW, Grillo WH, Hollowell GP, Hennington BS, Staub NL, Delesalle VA, Lello D, Merritt RB, Griffin GD, Bradford C, Mao J, Blumer LS, White SL 2017 Multiinstitutional, multidisciplinary study of the impact of course-based research experiences J Microbiol Biol Educ 18 10.1128/jmbe.v18i2.1317 http://dx.doi.org/10.1128/jmbe.v18i2.1317
11. Spell RM, Guinan JA, Miller KR, Beck CW 2014 Redefining authentic research experiences in introductory biology laboratories and barriers to their implementation CBE Life Sci Educ 13 102 110 10.1187/cbe.13-08-0169 24591509 3940451 http://dx.doi.org/10.1187/cbe.13-08-0169
12. Centers for Disease Control and Prevention 2013 Antibiotic resistance threats in the United States, 2013 Centers for Disease Control and Prevention, US Department of Health and Human Services
13. World Health Organization 2014 Antimicrobial resistance: global report on surveillance World Health Organization
14. Allen HK, Donato J, Wang HH, Cloud-Hansen KA, Davies J, Handelsman J 2010 Call of the wild: antibiotic resistance genes in natural environments Nat Rev Microbiol 8 251 259 10.1038/nrmicro2312 20190823 http://dx.doi.org/10.1038/nrmicro2312
15. Berendonk TU, Manaia CM, Merlin C, Fatta-Kassinos D, Cytryn E, Walsh F, Bürgmann H, Sørum H, Norström M, Pons MN 2015 Tackling antibiotic resistance: the environmental framework Nat Rev Microbiol 13 310 317 10.1038/nrmicro3439 25817583 http://dx.doi.org/10.1038/nrmicro3439
16. Kummerer K 2004 Resistance in the environment J Antimicrob Chemother 54 311 320 10.1093/jac/dkh325 15215223 http://dx.doi.org/10.1093/jac/dkh325
17. Walsh TR, Weeks J, Livermore DM, Toleman MA 2011 Dissemination of NDM-1 positive bacteria in the New Delhi environment and its implications for human health: an environmental point prevalence study Lancet Infect Dis 11 355 362 10.1016/S1473-3099(11)70059-7 21478057 http://dx.doi.org/10.1016/S1473-3099(11)70059-7
18. Walsh TR, Wu Y 2016 China bans colistin as a feed additive for animals Lancet Infect Dis 16 1102 1103 10.1016/S1473-3099(16)30329-2 27676338 http://dx.doi.org/10.1016/S1473-3099(16)30329-2
19. Wellington EM, Boxall AB, Cross P, Feil EJ, Gaze WH, Hawkey PM, Johnson-Rollings AS, Jones DL, Lee NM, Otten W 2013 The role of the natural environment in the emergence of antibiotic resistance in gram-negative bacteria Lancet Infect Dis 13 155 165 10.1016/S1473-3099(12)70317-1 23347633 http://dx.doi.org/10.1016/S1473-3099(12)70317-1
20. Giger W, Alder AC, Golet EM, Kohler HPE, McArdell CS, Molnar E, Siegrist H, Suter MJF 2003 Occurrence and fate of antibiotics as trace contaminants in wastewaters, sewage sludges, and surface waters CHIMIA Int J Chem 57 485 491 10.2533/000942903777679064 http://dx.doi.org/10.2533/000942903777679064
21. Kummerer K 2009 Antibiotics in the aquatic environment—a review—part II Chemosphere 75 435 441 10.1016/j.chemosphere.2008.12.006 http://dx.doi.org/10.1016/j.chemosphere.2008.12.006
22. Kümmerer K 2001 Drugs in the environment: emission of drugs, diagnostic aids and disinfectants into wastewater by hospitals in relation to other sources—a review Chemosphere 45 957 969 10.1016/S0045-6535(01)00144-8 http://dx.doi.org/10.1016/S0045-6535(01)00144-8
23. Ternes T, Joss A, Siegrist H 2004 Scrutinizing pharmaceuticals and personal care products in wastewater treatment Env Sci Technol 38 392A 399A 10.1021/es040639t http://dx.doi.org/10.1021/es040639t
24. Fick J, Söderström H, Lindberg RH, Phan C, Tysklind M, Larsson D 2009 Contamination of surface, ground, and drinking water from pharmaceutical production Env Toxicol Chem 28 2522 2527 10.1897/09-073.1 http://dx.doi.org/10.1897/09-073.1
25. Goel S 2015 Antibiotics in the environment: a review 19 42 Emerging micro-pollutants in the environment: occurrence, fate, and distribution ACS Symposium Series, vol. 1198 American Chemical Society
26. Kummerer K 2009 Antibiotics in the aquatic environment—a review—part I Chemosphere 75 417 434 10.1016/j.chemosphere.2008.11.086 http://dx.doi.org/10.1016/j.chemosphere.2008.11.086
27. Larsson DG, de Pedro C, Paxeus N 2007 Effluent from drug manufactures contains extremely high levels of pharmaceuticals J Hazard Mater 148 751 755 10.1016/j.jhazmat.2007.07.008 17706342 http://dx.doi.org/10.1016/j.jhazmat.2007.07.008
28. Li D, Yang M, Hu J, Zhang Y, Chang H, Jin F 2008 Determination of penicillin G and its degradation products in a penicillin production wastewater treatment plant and the receiving river Water Res 42 307 317 10.1016/j.watres.2007.07.016 http://dx.doi.org/10.1016/j.watres.2007.07.016
29. Sarmah AK, Meyer MT, Boxall AB 2006 A global perspective on the use, sales, exposure pathways, occurrence, fate and effects of veterinary antibiotics (VAs) in the environment Chemosphere 65 725 759 10.1016/j.chemosphere.2006.03.026 16677683 http://dx.doi.org/10.1016/j.chemosphere.2006.03.026
30. Doyle MP, Busta FF, Cords B, Davidson P, Hawke J, Hurd H, Isaacson R, Matthews K, Maurer J, Meng J 2006 Antimicrobial resistance: implications for the food system Compr Rev Food Sci Food Saf 5 71 137 10.1111/j.1541-4337.2006.00004.x http://dx.doi.org/10.1111/j.1541-4337.2006.00004.x
31. Berglund B 2015 Environmental dissemination of antibiotic resistance genes and correlation to anthropogenic contamination with antibiotics Infect Ecol Epidemiol 5 28564 10.3402/iee.v5.28564 26356096 4565060 http://dx.doi.org/10.3402/iee.v5.28564
32. Martinez JL 2012 Natural antibiotic resistance and contamination by antibiotic resistance determinants: the two ages in the evolution of resistance to antimicrobials Front Microbiol 3 1 10.3389/fmicb.2012.00001 22275914 3257838 http://dx.doi.org/10.3389/fmicb.2012.00001
33. Woolhouse M, Ward M, van Bunnik B, Farrar J 2015 Antimicrobial resistance in humans, livestock and the wider environment Philos Trans R Soc Lond B Biol Sci 370 20140083 10.1098/rstb.2014.0083 25918441 4424433 http://dx.doi.org/10.1098/rstb.2014.0083
34. Levy SB, FitzGerald GB, Macone AB 1976 Changes in intestinal flora of farm personnel after introduction of a tetracycline-supplemented feed on a farm New Engl J Med 295 583 588 10.1056/NEJM197609092951103 950974 http://dx.doi.org/10.1056/NEJM197609092951103
35. Birosova L, Mackulak T, Bodik I, Ryba J, Skubak J, Grabic R 2014 Pilot study of seasonal occurrence and distribution of antibiotics and drug resistant bacteria in wastewater treatment plants in Slovakia Sci Total Environ 490 440 444 10.1016/j.scitotenv.2014.05.030 24867706 http://dx.doi.org/10.1016/j.scitotenv.2014.05.030
36. Flores Ribeiro A, Bodilis J, Alonso L, Buquet S, Feuilloley M, Dupont JP, Pawlak B 2014 Occurrence of multi-antibiotic resistant Pseudomonas spp. in drinking water produced from karstic hydrosystems Sci Total Environ 490 370 378 10.1016/j.scitotenv.2014.05.012 24875257 http://dx.doi.org/10.1016/j.scitotenv.2014.05.012
37. Vaz-Moreira I, Nunes OC, Manaia CM 2012 Diversity and antibiotic resistance in Pseudomonas spp. from drinking water Sci Total Environ 426 366 374 10.1016/j.scitotenv.2012.03.046 22521167 http://dx.doi.org/10.1016/j.scitotenv.2012.03.046
38. Emmert EAB ASM Task Committee on Laboratory Biosafety 2013 Biosafety guidelines for handling microorganisms in the teaching laboratory: development and rationale J Microbiol Biol Educ 14 78 83 10.1128/jmbe.v14i1.531 23858356 3706168 http://dx.doi.org/10.1128/jmbe.v14i1.531

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2018-10-31
2019-04-21

Abstract:

Course-based research experiences (CREs) have been proposed as an inclusive model to expose all students, including those at institutions without a strong research infrastructure, to research at an early stage. Converting an entire semester-long course can be time consuming for instructors and expensive for institutions, so we have developed a short CRE that can be implemented in a variety of life science course types. The Prevalence of Antibiotic Resistance in the Environment (PARE) project uses common microbiology methods and equipment to engage students in nationwide surveillance of environmental soil samples to document the prevalence of antibiotic-resistant bacteria. The project has been implemented at institutions ranging from community colleges to doctoral-granting institutions in 30 states plus Puerto Rico. Programmatic feedback was obtained from instructors over three iterations, and revisions were made based on this feedback. Student learning was measured by pre/post assessment in a subset of institutions. Outcomes indicate that students made significant gains in the project learning goals. Journal of Microbiology & Biology Education

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Figures

Image of FIGURE 1

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FIGURE 1

Overview of the PARE methods. PARE = prevalence of antibiotic resistance in the environment.

Source: J. Microbiol. Biol. Educ. October 2018 vol. 19 no. 3 doi:10.1128/jmbe.v19i3.1603
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Image of FIGURE 2

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FIGURE 2

Sample data—serial dilution plating sets. A) Nutrient agar. The top photo shows the entire plating series. The bottom images show individual plates with diverse colony morphology rendering accurate enumeration difficult. B) MacConkey agar. The bottom images show the more uniform colony morphology of MacConkey agar.

Source: J. Microbiol. Biol. Educ. October 2018 vol. 19 no. 3 doi:10.1128/jmbe.v19i3.1603
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Image of FIGURE 3

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FIGURE 3

Student learning after the PARE module. Bars represent average scores with standard mean error. Pre and post tests are identical, and scores are out of 13.25 possible points. A paired -test reveals a significant ( < 0.0001) effect of the PARE experience on post test scores. = 43, **** < 0.0001. PARE = prevalence of antibiotic resistance in the environment.

Source: J. Microbiol. Biol. Educ. October 2018 vol. 19 no. 3 doi:10.1128/jmbe.v19i3.1603
Download as Powerpoint

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