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Biofilm Development and Its Genetic Control

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  • Authors: Jigar V. Desai1, Aaron P. Mitchell2
  • Editors: Mahmoud Ghannoum3, Matthew Parsek4, Marvin Whiteley5, Pranab Mukherjee6
  • VIEW AFFILIATIONS HIDE AFFILIATIONS
    Affiliations: 1: Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213; 2: Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213; 3: Case Western Reserve University, Cleveland, OH; 4: University of Washington, Seattle, WA; 5: University of Texas at Austin, Austin, TX; 6: Case Western Reserve University, Cleveland, OH
  • Source: microbiolspec June 2015 vol. 3 no. 3 doi:10.1128/microbiolspec.MB-0005-2014
  • Received 06 August 2014 Accepted 04 September 2014 Published 05 June 2015
  • Aaron Mitchell, apm1@cmu.edu
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  • Abstract:

    The fungus is a major source of device-associated infection because of its capacity for biofilm formation. It is part of the natural mucosal flora and thus has access to available niches that can lead to infection. In this chapter we discuss the major properties of biofilms and the insight that has been gleaned from their genetic determinants. Our specific areas of focus include biofilm structure and development, cell morphology and biofilm formation, biofilm-associated gene expression, the cell surface and adherence, the extracellular matrix, biofilm metabolism, and biofilm drug resistance.

  • Citation: Desai J, Mitchell A. 2015. Biofilm Development and Its Genetic Control. Microbiol Spectrum 3(3):MB-0005-2014. doi:10.1128/microbiolspec.MB-0005-2014.

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Cell Wall Proteins
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/content/journal/microbiolspec/10.1128/microbiolspec.MB-0005-2014
2015-06-05
2017-09-23

Abstract:

The fungus is a major source of device-associated infection because of its capacity for biofilm formation. It is part of the natural mucosal flora and thus has access to available niches that can lead to infection. In this chapter we discuss the major properties of biofilms and the insight that has been gleaned from their genetic determinants. Our specific areas of focus include biofilm structure and development, cell morphology and biofilm formation, biofilm-associated gene expression, the cell surface and adherence, the extracellular matrix, biofilm metabolism, and biofilm drug resistance.

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FIGURE 1

Confocal micrographic images of a biofilm. These images present a biofilm grown in yeast extract-peptone-dextrose medium at 37°C. The sample was prepared by embedding and staining with Alexafluor 594-conjugated Concanavalin A, using a procedure modified from reference 83 . (A) Side projection view. Hyphae are clearly visible in the upper portion of the biofilm, as are aggregates of brightly stained extracellular material. A color scale bar represents the 270-micron depth and indicates the pseudocolor scale used for apical projections. (B) Apical projection of basal (substrate-proximal) 50-micron region. A yeast cell layer is evident from the substrate level (red) to 50 microns above the substrate (blue). A few hyphae or pseudohyphae are visible as well. Some amorphous extracellular material is apparent. (C) Apical projection of the entire biofilm. Hyphae are visible above the basal layer, extending from ∼150 microns (green) to 270 microns (red) above the substrate. Yeast cells are seen in clusters at the ends of hyphae. (D) Three-dimensional reconstruction of the biofilm sample. Hyphae at the top of the biofilm are readily visible above the dense basal region. doi:10.1128/microbiolspec.MB-0005-2014.f1

Source: microbiolspec June 2015 vol. 3 no. 3 doi:10.1128/microbiolspec.MB-0005-2014
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