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Bacterial Extracellular Polysaccharides in Biofilm Formation and Function

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  • Authors: Dominique H. Limoli1, Christopher J. Jones2, Daniel J. Wozniak3
  • Editors: Mahmoud Ghannoum4, Matthew Parsek5, Marvin Whiteley6, Pranab Mukherjee7
  • VIEW AFFILIATIONS HIDE AFFILIATIONS
    Affiliations: 1: Department of Microbial Infection and Immunity, Ohio State University, Columbus, OH 43210; 2: Department of Microbiology and Environmental Toxicology, University of California Santa Cruz, Santa Cruz, CA 95064; 3: Department of Microbial Infection and Immunity, Ohio State University, Columbus, OH 43210; 4: Case Western Reserve University, Cleveland, OH; 5: University of Washington, Seattle, WA; 6: University of Texas at Austin, Austin, TX; 7: Case Western Reserve University, Cleveland, OH
  • Source: microbiolspec June 2015 vol. 3 no. 3 doi:10.1128/microbiolspec.MB-0011-2014
  • Received 09 September 2014 Accepted 19 November 2014 Published 26 June 2015
  • Daniel J. Wozniak, daniel.wozniak@osumc.edu
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  • Abstract:

    Microbes produce a biofilm matrix consisting of proteins, extracellular DNA, and polysaccharides that is integral in the formation of bacterial communities. Historical studies of polysaccharides revealed that their overproduction often alters the colony morphology and can be diagnostic in identifying certain species. The polysaccharide component of the matrix can provide many diverse benefits to the cells in the biofilm, including adhesion, protection, and structure. Aggregative polysaccharides act as molecular glue, allowing the bacterial cells to adhere to each other as well as surfaces. Adhesion facilitates the colonization of both biotic and abiotic surfaces by allowing the bacteria to resist physical stresses imposed by fluid movement that could separate the cells from a nutrient source. Polysaccharides can also provide protection from a wide range of stresses, such as desiccation, immune effectors, and predators such as phagocytic cells and amoebae. Finally, polysaccharides can provide structure to biofilms, allowing stratification of the bacterial community and establishing gradients of nutrients and waste products. This can be advantageous for the bacteria by establishing a heterogeneous population that is prepared to endure stresses created by the rapidly changing environments that many bacteria encounter. The diverse range of polysaccharide structures, properties, and roles highlight the importance of this matrix constituent to the successful adaptation of bacteria to nearly every niche. Here, we present an overview of the current knowledge regarding the diversity and benefits that polysaccharide production provides to bacterial communities within biofilms.

  • Citation: Limoli D, Jones C, Wozniak D. 2015. Bacterial Extracellular Polysaccharides in Biofilm Formation and Function. Microbiol Spectrum 3(3):MB-0011-2014. doi:10.1128/microbiolspec.MB-0011-2014.

Key Concept Ranking

Bacterial Polysaccharides
0.4773688
Rcs Phosphorelay System
0.46907225
Type IV Pili
0.45289737
Urinary Tract Infections
0.42861962
Pseudomonas aeruginosa
0.4061699
0.4773688

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/content/journal/microbiolspec/10.1128/microbiolspec.MB-0011-2014
2015-06-26
2017-08-19

Abstract:

Microbes produce a biofilm matrix consisting of proteins, extracellular DNA, and polysaccharides that is integral in the formation of bacterial communities. Historical studies of polysaccharides revealed that their overproduction often alters the colony morphology and can be diagnostic in identifying certain species. The polysaccharide component of the matrix can provide many diverse benefits to the cells in the biofilm, including adhesion, protection, and structure. Aggregative polysaccharides act as molecular glue, allowing the bacterial cells to adhere to each other as well as surfaces. Adhesion facilitates the colonization of both biotic and abiotic surfaces by allowing the bacteria to resist physical stresses imposed by fluid movement that could separate the cells from a nutrient source. Polysaccharides can also provide protection from a wide range of stresses, such as desiccation, immune effectors, and predators such as phagocytic cells and amoebae. Finally, polysaccharides can provide structure to biofilms, allowing stratification of the bacterial community and establishing gradients of nutrients and waste products. This can be advantageous for the bacteria by establishing a heterogeneous population that is prepared to endure stresses created by the rapidly changing environments that many bacteria encounter. The diverse range of polysaccharide structures, properties, and roles highlight the importance of this matrix constituent to the successful adaptation of bacteria to nearly every niche. Here, we present an overview of the current knowledge regarding the diversity and benefits that polysaccharide production provides to bacterial communities within biofilms.

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FIGURE 1

Adapted representative chemical structures of polysaccharides which participate in biofilm formation including (A) polysaccharide intercellular adhesin (PIA), (B) Psl, (C) alginate, capsular polysaccharide (CPS) from (Di) and (Dii) , (E) levan, (F) cellulose, and (G) colanic acid. Brackets depict repeating units. doi:10.1128/microbiolspec.MB-0011-2014.f1

Source: microbiolspec June 2015 vol. 3 no. 3 doi:10.1128/microbiolspec.MB-0011-2014
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FIGURE 2

Colony phenotypes conferred upon expression or overexpression of PS by representative bacteria. (A) PS intercellular adhesion producing . Reprinted from ( 214 ) with permission from the publisher. (B) Pel producing Δ). Reprinted from ( 215 ) with permission from the publisher. (C) Psl producing Δ). Reprinted from ( 215 ) with permission from the publisher. (D) Alginate overproducing (). Not previously published. Credit: Daniel Wozniak. (E) Colanic acid producing . Reprinted from ( 216 ) with permission from the publisher. (F) VPS producing rugose variant of . Reprinted from ( 66 ) with permission from the publisher. (G) EPS producing . Reprinted from ( 217 ) with permission from the publisher. (H) Cellulose producing (). Reprinted from ( 218 ) with permission from the publisher. doi:10.1128/microbiolspec.MB-0011-2014.f2

Source: microbiolspec June 2015 vol. 3 no. 3 doi:10.1128/microbiolspec.MB-0011-2014
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TABLE 1

Summary of the cellular location, chemical composition, and functions of bacterial polysaccharides important for biofilm formation

Source: microbiolspec June 2015 vol. 3 no. 3 doi:10.1128/microbiolspec.MB-0011-2014

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