Color Plates
Category: Clinical Microbiology

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Figures

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Color Plate 1.
Detection of CMV antigens in infected MRC-5 cells. Following shell vial culture, cells are stained with fluorescently labeled antibodies which detect CMV immediate-early antigen. Original magnification, × 200.

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Color Plate 2.
Biopsy specimen from a patient with CMV colitis, showing a classic “owl-eye” intranuclear inclusion (arrow) and intracytoplasmic inclusions. The dense intranuclear inclusion with surrounding halo is formed when the mass of viral particles shrinks away from the nuclear membrane during fixation. While herpes simplex virus intranuclear inclusions can have a similar appearance, CMV is the only member of the family Herpesviridae that contains both intranuclear and intracytoplasmic inclusions. The section was stained with hematoxylin and eosin stain. Original magnification, × 1,000 (oil immersion). (Courtesy of Bobbi Pritt.)

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Color Plate 3.
Immunoperoxidase staining of CMV antigens in a biopsy from a patient with CMV colitis. Viral inclusions stain brown (blue counterstain). Original magnification, × 1,000 (oil immersion). (Courtesy of Bobbi Pritt.)

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Color Plate 4.
Induced BCBL-1 cells stained with HHV-8-negative sera (A) or HHV-8-positive sera (B).

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Color Plate 5.
Untreated BCBL-1 cells stained with HHV-8-negative sera (A) or anti-LANA-1 antibody (B).

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Color Plate 6.
Hematoxylin- and eosinstained section of liver showing reactive changes and intranuclear inclusions of adenovirus. (Photograph courtesy of Armed Forces Institute of Pathology, Washington, DC.)

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Color Plate 7.
Fluorescent yeast cells from blood culture bottles with a Gram stain positive for yeast following testing with species-specific PNA FISH probes. (Left) Green fluorescent cells after reaction with C. albicans-specific PNA FISH probe. (Right) Red fluorescent cells after reaction with C. glabrata-specific probe.

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Color Plate 8.
Gram-positive, filamentous, branching bacilli characteristic of the Nocardia species, as seen directly in a bronchoalveolar lavage specimen. Magnification, × 1,200.

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Color Plate 9.
MAFS-positive, filamentous, branching bacilli characteristic of the Nocardia species, as seen directly in a bronchoalveolar lavage specimen. Magnification, × 1,200.

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Color Plate 10.
Mycobacterial spindle cell pseudotumor in a woman with previous AIDS and M. avium infection. (A) Spindle cell proliferation (hematoxylin and eosin stain). Magnification, × 200. (B) Acid-fast bacilli (Ziehl-Neelsen stain). Magnification, × 400. (C) Macrophage origin of the spindle cells (immunohistochemical stain with marker CD68). Magnification, × 400. Courtesy of Michael Deavers, M.D.