Chapter 3 : Lactobacilli

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This chapter discusses biology of effector organism such as lactobacilli for probiotic and replacement therapy. It talks about metabolism, nutritional requirements, natural habitats, and taxonomy of lactobacilli. The genus has been studied extensively and is now a well-characterized genus in the lactic acid bacteria (LAB) group, which is composed of more than 100 recognized species. The genus is of interest for a number of reasons: its long history of safe use in the fermentation and preservation of traditional foods (dairy, meat, and vegetable products) and more recently its incorporation in functional probiotic foods, as well as its ubiquitous presence in human and animal microbiotas, especially in the gastrointestinal and genitourinary tracts (where it dominates the vaginal microbiota). However, the main interest is undoubtedly its role as a probiotic and as a biotherapeutic agent in some clinical situations. spp. are among the most frequent and better characterized microorganisms used as a probiotic. Important considerations in the choice of a probiotic include safety, functional aspects, and technological aspects. Probiotics are being commercially developed for both human and animal consumption, especially in the poultry and aquaculture industries. Their application constitutes alternatives to antibiotics, as well as acting as prophylactics, in particular in the prevention of gastrointestinal infections. Some commercially probiotic products containing lactobacilli are listed in the chapter. The chapter talks about the use of lactobacilli in food, industrial, and medical applications.

Citation: Barbés C. 2008. Lactobacilli, p 19-33. In Versalovic J, Wilson M (ed), Therapeutic Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555815462.ch3
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1. Antonio,, M. A. D., S. E. Hawes, and, S. L. Hillier. 1999. The identification of vaginal Lactobacillus species and the demographic and microbiologic characteristics of women colonized by these species. J. Infect. Dis. 180: 19501956.
2. Barbés, C. 2001. Microbiota and gastrointestinal system. Rev. Esp. Enferm. Dig. 93: 328330.
3. Beijerinck,, M. W. 1901. Anhäufungsversuche mit Ureumbakterien: Ureumspaltung durch Urease und durch Katabolismus. Zentbl. Bakteriol. Parasitenkd. Infektkrankh. Hyg. Abt. 2 7: 3361.
4. Boris, S.,, J. E. Suárez,, F. Vázquez, and, C. Barbés. 1998. Adherence of human vaginal lactobacilli to vaginal epithelial cells and interaction with uropathogens. Infect. Immun. 66: 19851989.
5. Boris, S., and, C. Barbés. 2000. Role played by lactobacilli in controlling the population of vaginal pathogens. Microbes Infect. 2: 543546.
6. Boyd,, M. A.,, M. A., D. Antonio, and, S. L. Hillier. 2005. Comparison of API 50 CH strips of whole-chromosomal DNA probes for identification of Lactobacillus species. J. Clin. Microbiol. 43: 53095311.
7. Brown, A., C., and, A. Valiere. 2004. Probiotics and medical nutrition therapy. Nutr. Clin. Care 7: 5658.
8. Caglar, E.,, B. Kargul, and, I. Tanboga. 2005. Bacteriotherapy and probiotics’ role on oral health. Oral Dis. 11: 131137.
9. Collins,, M. D.,, U. Rodrigues,, C. Ash,, M. Aguirre,, J. A., E. Farrow,, A. Martinez-Murcia,, B. A. Phillips,, A. M. Williams, and, S. Wallbanks. 1991. Phylogenetic analysis of the Genus Lactobacillus and related lactic acid bacteria as determined by reverse transcriptase sequencing of 16S rRNA. FEMS Microbiol. Lett. 77: 512.
10. Dairy Council of California. 2000. Probiotics—friendly bacteria with a host of benefits. Dairy Council of California, Sacramento, CA. http:www//dairycouncilofca.org/PDFs/probiotics.pdf.
11. Dellaglio, F., and, G. E. Felis. 2005. Taxonomy of lactobacilli and bifidobacteria, p. 25–49. In G. W. Tannock (ed.), Probiotics and Prebiotics: Scientific Aspects. Academic Press, Norfolk, United Kingdom.
12. De Vuyst, L., and, B. Degeest. 1999. Heteropolysaccharides from lactic acid bacteria. FEMS Microbiol. Rev. 23: 153157.
13. Donohue,, D. C.,, S. Salminen, and, P. Marteau. 1998. Safety of probiotic bacteria, p. 369–384. In S. Salminen and, A. von Wright (ed.), Lactic Acid Bacteria. Microbiology and Functional Aspects. Marcel Dekker, Inc., New York, NY.
14. Dykes,, G. A., and, A. von Holy. 1994. Strain typing in the genus Lactobacillus. Lett. Appl. Microbiol. 19: 6366.
15. Euzéby,, J. P. 2007. List of procaryotic names with standing in nomenclature. http://www.bacterio.cict.fr/twothousand/twothousandsix.htlm.
16. Famularo, G.,, M. Pieluigi,, R. Coccia,, P. Mastroiacovo, and, C. De Simone. 2001. Microecology, bacterial vaginosis and probiotics: perspectives for bacteriotherapy. Med. Hyp. 56: 421430.
17. Fasoli,, F.,, M. Marzotto,, L. Rizzotti,, F. Rossi,, F. Dellaglio, and, S. Torriani. 2003. Bacterial composition of commercial probiotic products as evaluated by PCR-DGGE analysis. Int. J. Food Microbiol. 82: 5970.
18. Fernández,, M. F.,, S. Boris, and, C. Barbés. 2003. Applications of probiotic human lactobacilli, p. 293–301. In S. G. Pandalai (ed.), Recent Research Developments in Infection and Immunity, vol. 1. Transworld Research Network, Fort P. O. Kerala, India.
19. Fuller, R. 1989. Probiotics in man and animals. J. Appl. Bacteriol. 66: 365378.
20. Garrity,, G. M.,, J. A. Bell, and, T. G. Lilbum. 2004. Taxonomic outline of the Prokaryotes. Bergey’s Manual of Systematic Bacteriology, 2nd ed. Release 5.0. Springer-Verlag, New York, NY.
21. Geis, A. 2003. Perspectives of genetic engineering of bacteria used in food fermentations, p. 100–118. In K. J. Heller (ed.), Genetically Engineered Food and Methods of Detection. Wiley-VCH, Weinheim, Germany.
22. Gill,, H. S. 1998. Stimulation of the immune system by lactic cultures. Int. Dairy J. 8: 535544.
23. Gionchetti, P.,, F. Rizzello, and, M. Campieri. 2002. Probiotics in gastroenterology. Curr. Opin. Gastroenterol. 17: 331335.
24. Gismondo,, M. R.,, L. Drago, and, A. Lombardi. 1999. Review of probiotics available to modified gastrointestinal flora. Int. J. Antimicrob. Agents 12: 287292.
25. Gorbach,, S. L. 2000. Probiotics and gastrointestinal health. Am. J. Gastroenterol. 95(Suppl. 1): S2–S4.
26. Guarino, A. 1998. Effects of probiotics in children with cystic fibrosis. Gastroenterol. Int. 11(Suppl.): 91.
27. Hammes,, W. P., and, C. Hertel. 2003. The genera Lactobacillus and Carnobacterium. In The Prokaryotes: An Evolving Electronic Resource for the Microbiological Community, 3rd ed. Springer-Verlag. New York, NY. http://link.springer.ny.com/link/service/books/10125/. Accessed 10 April 2007.
28. Hilton, E., and, P. Rindos. 1995. Lactobacillus GG vaginal suppositories and vaginitis. J. Clin. Microbiol. 33:1433.
29. Ho,, P. S.,, J. Kwang, and, Y. K. Lee. 2005. Intragastric administration of Lactobacillus casei expressing transmissible gastroenteritis coronavirus spike glycoprotein induced specific antibody production. Vaccine 23: 13351342.
30. Holzapfel,, W. H.,, P. Haberer,, R. Geisen,, J. Björkroth, and, U. Schillinger. 2001. Taxonomy and important features of probiotic microorganisms in food and nutrition. Am. J. Clin. Nutr. 73(Suppl.): S365S373.
31. Hughes,, V. L., and, S. L. Hillier. 1990. Microbiologic characteristics of Lactobacillus products used for colonization of the vagina. Obstet. Gynecol. 75: 244248.
32. Huys,, G.,, M. Vancanneyt,, K. D’Haene,, V. Vankerckhoven,, H. Goossens, and, J. Swings. 2006. Accuracy of species identity of commercial bacterial cultures intended for probiotic or nutritional use. Res. Microbiol. 157: 803810.
33. Jin,, L.,, L. Tao,, S. I. Pavlova,, J. S. So,, N. Kiwanuka,, Z. Namukwaya,, B. A. Saberbein, and, M. Wawer. 2007. Species diversity and relative abundance of vaginal lactic acid bacteria from women in Uganda and Korea. J. Appl. Microbiol. 102: 11071115.
34. Johnson,, J. L.,, C. F. Phelps,, C. S. Cummins,, J. London, and, F. Gasser. 1980. Taxonomy of the Lactobacillus acidophilus group. Int. Syst. Bacteriol. 30: 5368.
35. Kalliomaki, M.,, S. Salminen,, T. Poussa,, H. Arvilommi, and, E. Isolauri. 2003. Probiotics and prevention of atopic disease: a 4 year follow up of a randomised placebo-controlled trial. Lancet 361: 18691871.
36. Kandler,, O.,, and N. Weiss. 1986. Regular non-sporing Gram-positive rods, p. 1208–1234. In P. H. Sneath,, N. Mair,, M. E. Sharpe, and, J. G. Holt (ed.), Bergey’s Manual of Systematic Bacteriology, vol. 2. William and Wilkins, Baltimore, MD.
37. Kaur,, I. P.,, K. Chopra, and, A. Saini. 2002. Probiotics: potential pharmaceutical applications. Eur. J. Pharm. Sci. 15: 19.
38. Land,, M. H.,, K. Rouster-Stevens,, C. R. Woods,, M. L. Cannon,, J. Cnota, and, A. K. Shetty. 2005. Lactobacillus sepsis associated with probiotic therapy. Pediatrics 115: 178181.
39. Lidbeck, A., and, C. E. Nord. 1993. Lactobacilli and normal human anaerobic microflora. Clin. Infect. Dis. 16:S181–S187.
40. Lidbeck, A.,, C. E. Nord,, J. A. Gustafsson, and, J. Rafter. 1992. Lactobacilli, anticarcinogenic activities and human intestinal microflora. Eur. J. Cancer Prev. 1: 341353.
41. Limdi,, J. K.,, C. O’Neill, and, J. McLaughlin. 2006. Do probiotics have a therapeutic role in gastroenterology? World J. Gastroenterol. 12: 54475457.
42. Maassen,, C. B.,, W. J. Boersma,, C. Holten-Neelen,, E. Claassen, and, J. D. Laman. 2003. Growth phase of orally administered Lactobacillus strains differentially affects IgG1/IGg2a ratio for soluble antigens: implications for vaccine development. Vaccine 21: 27512757.
43. Mackay,, A. D.,, M. B. Taylor,, C. C. Kibbler, and, J. M. T. Hamilton-Miller. 1999. Lactobacillus endocarditis caused by a probiotic organism. Clin. Microbiol. Infect. 5: 290292.
44. Mäkinen,, A. M., and, M. Bigret. 1998. Safety of probiotic bacteria, p. 73-102. In S. Salminen and, A. von Wright (ed.), Lactic Acid Bacteria. Microbiology and Functional Aspects. Marcel Dekker, Inc., New York, NY.
45. Malin, M.,, P. Verronen,, H. Mykkanen,, S. Salminen, and, E. Isolauri. 1996. Increased bacterial urease activity in faeces in juvenile chronic arthritis: evidence of altered intestinal microflora. Br. J. Rheumatol. 35: 689694.
46. Marteau,, P. R. 2002. Probiotics in clinical conditions. Clin. Rev. Allergy. Immunol. 22: 255274.
47. Marteau,, P. R.,, M. de Brese,, C. J. Cellier, and, J. Schrezenmeir. 2001. Protection from gastrointestinal diseases with the use of probiotics. Am. J. Clin. Nutr. 73(Suppl.): S430S436.
48. Martin,, H. L.,, B. A. Richardson,, P. M. Nyange,, L. Lavreys,, S. L. Hillier,, B. Chohan,, K. Mandaliya,, J. O. Ndinya-Achola,, J. Bwayo, and, J. Kreiss. 1999. Vaginal lactobacilli, microbial flora, and risk of human immunodeficiency virus type 1 and sexually transmitted disease acquisition. J. Infect. Dis. 180: 18631868.
49. Mehta,, A.,, J. Talwalkar,, C. V. Shetty,, and N. D. Motashaw. 1995. Microbial flora of the vagina, p. 1–7. In A. B. Onderdonk,, P. J. Heidt, and, V. C. Rusch (ed.), Microecology and Therapy, vol. 23. Institut für Mikroökologie, Herborn-Dill, Germany.
50. Mikelsaar, M.,, R. Mändar, and, E. Seppo. 1998. Lactic acid microflora in the human microbial ecosystem and its development, p. 279–342. In S. Salminen and Atte von Wright (ed.), Lactic Acid Bacteria: Microbiology and Functional Aspects, 2nd ed. Marcel Dekker, New York, NY.
51. Mitsuoka, T. 1992. Intestinal flora and aging. Nutr. Rev. 50: 438446.
52. Mohler,, R. W., and, C. P. Brown. 1933. Doderlein’s bacillus in the treatment of vaginitis. Am. J. Obstet. Gynecol. 25: 718723.
53. Morishita, T.,, Y. Deguchi,, M. Yajima,, T. Saskurai, and, T. Yura. 1981. Multiple nutritional requirements of lactobacilli: genetic lesions affecting amino acid biosynthetic pathways. J. Bacteriol. 148: 6471.
54. Nyirjesy, P.,, M. V. Weitz,, M. H. T. Grody, and, B. Lorber. 1997. Over-the-counter and alternative medicines in the treatment of chronic vaginal symptoms. Obstet. Gynecol. 90: 5053.
55. Penner, R.,, R. N. Fedorak, and, K. L. Madsen. 2005. Probiotics and nutraceuticals: non-medicinal treatments of gastrointestinal diseases. Curr. Opin. Pharmacol. 5: 596603.
56. Perdigon, G.,, R. Fuller, and, R. Raya. 2001. Lactic acid bacteria and their effect on the immune system. Curr. Issues Intest. Microbiol. 2: 2742.
57. Rautio,, M.,, H. Jousimies-Somer,, H. Kauma,, I. Pietarinen,, M. Saxelin,, S. Tynkkynen, and, M. Koskela. 1999. Liver abscess due to a Lactobacillus rhamnosus strain indistinguishable from L. rhamnosus strain GG. Clin. Infect. Dis. 28: 11601161.
58. Reid, G. 2001. Probiotic agents to protect the urogenital tract against infection. Am. J. Clin. Nutr. 73(Suppl.): S437S443.
59. Reid, G. 2002. Probiotics for urogenital health. Nutr. Clin. Care 5: 38.
60. Reid,, G.,, A. V. Bruce,, N. Fraser,, G. H. Heinemann,, J. Owen, and, B. Henning. 2001. Oral probiotics can resolve urogenital infections. FEMS Immunol. Med. Microbiol. 30: 4952.
61. Reid, G., and, J. A. Hammond. 2005. Probiotics. Some evidence for their effectiveness. Can. Fam. Phys. 51: 14871493.
62. Roberfroid,, M. B. 1998. Prebiotics and synbiotics: concepts and nutritional properties. Br. J. Nutr. 80:S197–S202.
63. Ross,, R. P.,, S. Morgan, and, C. Hill. 2002. Preservation and fermentation: past, present and future. Int. J. Food Microbiol. 79: 316.
64. Sakamoto,, I.,, M. Igarashi,, K. Kimura,, A. Takagi,, T. Miwa, and, Y. Koga. 2001. Suppressive effect of Lactobacillus gasseri OLL 2716 (LG21) on Helicobacter pylori infection in humans. J. Antimicrob. Chemother. 47: 709710.
65. Salminen, S.,, M. A. Deighton,, Y. Benno, and, S. L. Gorbach. 1998. Lactic acid bacteria in health and disease, p. 211–253. In S. Salminen and, A. von Wright (ed.), Lactic Acid Bacteria. Microbiology and Functional Aspects. Marcel Dekker, Inc., New York, NY.
66. Sanders,, M. E., and, J. H. Veld. 1999. Bringing a probiotic-containing functional food to the market: microbiological, product regulatory and labelling issues. Antonie Leeuwenhock 76: 293315.
67. Schleifer,, K. H., and, W. Ludwig. 1995. Phylogeny of the Genus Lactobacillus and related genera. Syst. Appl. Microbiol. 18: 461467.
68. Schrezenmeir, J., and, M. de Vrese. 2001. Probiotics, prebiotics and symbiotics, approaching a definition. Am. J. Clin. Nutr. 73(Suppl.): S361S364.
69. Schultz, M., and, R. B. Sartor. 2000. Probiotics and inflammatory bowel diseases. Am. J. Gastroenterol. 95(Suppl. 1): S19–S21.
70. Seegers,, J. F. 2002. Lactobacilli as live vaccine delivery vectors: progress and prospects. Trends Biotechnol. 20: 508515.
71. Senok,, A. C.,, A. Y. Ismaeel, and, G. A. Botta. 2005. Probiotics: facts and myths. Clin. Mirobiol. Infect. 11: 958966.
72. Shareck, J.,, Y. Choi,, B. Lee, and, C. B. Miguez. 2004. Cloning vectors based on cryptic plasmids isolated from lactic acid bacteria: their characteristics and potential applications in biotechnology. Crit. Rev. Biotechnol. 24: 155208.
73. Sharpe,, M. E. 1981. The genus Lactobacillus, p. 1653–1679. In M. P. Starr,, H. Stolp,, H. G. Trüper,, A. Balows, and, H. G. Schlegel (ed.), The Prokaryotes. Springer-Verlag, Berlin, Germany.
74. Shetty,, A. K., and, C. R. Woods. 2005. Lactobacillus sepsis associated with probiotic therapy: in reply. Pediatrics 116: 517518.
75. Sieber, R., and, U.-T. Dietz. 1998. Lactobacillus acidophilus and yogurt in the prevention and therapy of bacterial vaginosis. Int. Dairy J. 8: 599607.
76. Song,, Y.,, N. Kato,, Y. Matsumiya,, C. Liu,, H. Kato, and, K. Watanabe. 1999. Identification of and hydrogen peroxide production by fecal and vaginal lactobacilli isolated from Japanese women and newborn infants. J. Clin. Microbiol. 37: 30623064.
77. Tagg,, J. R.,, A. S. Dajani, and, L. W. Watanabe. 1976. Bacteriocins of gram-positive bacteria. Bacteriol. Rev. 40: 722756.
78. Takano, T. 1998. Milk derived peptides and hypertension reduction. Int. Dairy J. 8: 375378.
79. Vallor,, A. C.,, M. A. D. Antonio,, S. E. Hawes, and, S. L. Hillier. 2001. Factors associated with acquisition of, or persistent colonization by, vaginal lactobacilli: role of hydrogen peroxide production. J. Infect. Dis. 184: 14311436.
80. Vanderhoof,, J. A. 2001. Probiotics: future directions. Am. J. Clin. Nutr. 115:2S–5S.
81. Velraeds, M.,, H. Van Der Mei,, G. Reid, and, H. Busscher. 1996. Inhibition of initial adhesion of uropathogenic Enterococcus faecalis by biosurfactants from Lactobacillus isolates. Appl. Environ. Microbiol. 62: 19581963.
82. Viljanen,, M.,, E. Savilahti,, T. Haahtela,, K. Juntunen-Backman,, R. Korpela,, T. Poussa,, T. Tuure, and, M. Kuitunen. 2005. Probiotics in the treatment of atopic eczema/dermatitis syndrome in infants: a double-blind placebo controlled trial. Allergy 60: 494500.
83. Yeung,, P. S. M.,, M. E. Sanders,, C. L. Kitts,, R. Cano, and, P. S. Tong. 2002. Species-specific identification of commercial probiotic strains. J. Dairy Sci. 85: 10391051.


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Table 1

Phylogenetic groups of the genus

Citation: Barbés C. 2008. Lactobacilli, p 19-33. In Versalovic J, Wilson M (ed), Therapeutic Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555815462.ch3
Generic image for table
Table 2

Some commercially probiotic products containing lactobacilli

Citation: Barbés C. 2008. Lactobacilli, p 19-33. In Versalovic J, Wilson M (ed), Therapeutic Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555815462.ch3

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