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Chapter 14 : Transcription-Mediated Amplification Assay for Detection of in Samples from Patients with Community-Acquired Pneumonia

Authors: Marie K. Hudspeth1, Kathleen Clark-Dickey2, Elizabeth M. Marlowe3, Laura G. Schindler4, Karen Campbell5, James T. Summersgill6
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Affiliations: 1: Gen-Probe Inc., San Diego, CA 92121; 2: Gen-Probe Inc., San Diego, CA 92121; 3: Gen-Probe Inc., San Diego, CA 92121; 4: Division of Infectious Diseases, Department of Medicine, University of Louisville School of Medicine, Louisville, KY 40292; 5: Division of Infectious Diseases, Department of Medicine, University of Louisville School of Medicine, Louisville, KY 40292; 6: Division of Infectious Diseases, Department of Medicine, University of Louisville School of Medicine, Louisville, KY 40292
Content Type: Monograph
Publication Year: 2006

Category: Bacterial Pathogenesis

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Transcription-Mediated Amplification Assay for Detection of in Samples from Patients with Community-Acquired Pneumonia, Page 1 of 2

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Abstract:

Diagnosis of acute in patients with community-acquired pneumonia can be time-consuming and difficult to confirm using current diagnostic tests. A research prototype assay using Gen-Probe's proprietary transcription-mediated amplification (TMA) technology was evaluated for detection of in throat swabs from patients with documented community-acquired pneumonia. The TMA assay procedure includes target capture, TMA, and the hybridization protection assay. Amplified methods are generally more sensitive than other laboratory methods, particularly for fastidious organisms such as . Therefore, in addition to the initial TMA assay that targets a specific region of rRNA, another target was utilized in an alternate region of rRNA as a confirmatory TMA-based assay.

Citation: Hudspeth M, Clark-Dickey K, Marlowe E, Schindler L, Campbell K, Summersgill J. 2006. Transcription-Mediated Amplification Assay for Detection of in Samples from Patients with Community-Acquired Pneumonia, p 53-54. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch14
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References

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1. Ramirez, J. A.,, S. Ahkee,, A. Tolentino,, R. D. Miller and, J.T. Summersgill. 1996. Diagnosis of Legionella pneumophila, Mycoplasma pneumoniae, or Chlamydia pneumoniae lower respiratory infection using the polymerase chain reaction on a single throat swab. Diagn. Microbiol. Infect. Dis. 24: 7– 14.

Tables

Transcription-Mediated Amplification Assay for Detection of Legionella pneumophila in Samples from Patients with Community-Acquired Pneumonia
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Citation: Hudspeth M, Clark-Dickey K, Marlowe E, Schindler L, Campbell K, Summersgill J. 2006. Transcription-Mediated Amplification Assay for Detection of in Samples from Patients with Community-Acquired Pneumonia, p 53-54. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch14
/content/10.1128/9781555815660.ch14.ch14tab01
TABLE 1
Results of PCR and TMA L. assays on community-acquired pneumonia patient samples
Generic image for table
TABLE 1

Results of PCR and TMA L. assays on community-acquired pneumonia patient samples

Citation: Hudspeth M, Clark-Dickey K, Marlowe E, Schindler L, Campbell K, Summersgill J. 2006. Transcription-Mediated Amplification Assay for Detection of in Samples from Patients with Community-Acquired Pneumonia, p 53-54. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch14

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