Molecular Comparison of Isolates from a Recurring Outbreak of Legionnaires’ Disease Spanning 22 Years

Robert F. Benson; Claressa E. Lucas; Ellen W. Brown; Karen D. Cowgill; Barry S. Fields

doi:10.1128/9781555815660.ch37

Chapter 37 : Molecular Comparison of Isolates from a Recurring Outbreak of Legionnaires’ Disease Spanning 22 Years

Authors: Robert F. Benson¹, Claressa E. Lucas², Ellen W. Brown³, Karen D. Cowgill⁴, Barry S. Fields⁵

VIEW AFFILIATIONS HIDE AFFILIATIONS

Affiliations: 1: Respiratory Disease Branch, Centers for Disease Control and Prevention, 1600 Clifton Rd., Atlanta, GA 30333; 2: Respiratory Disease Branch, Centers for Disease Control and Prevention, 1600 Clifton Rd., Atlanta, GA 30333; 3: Respiratory Disease Branch, Centers for Disease Control and Prevention, 1600 Clifton Rd., Atlanta, GA 30333; 4: Seattle Biomedical Research Institute, 307 Westlake Ave N, Suite 500, Seattle WA 98109; 5: Respiratory Disease Branch, Centers for Disease Control and Prevention, 1600 Clifton Rd., Atlanta, GA 30333

Content Type: Monograph

Publication Year: 2006

Category: Bacterial Pathogenesis

Book DOI: 10.1128/9781555815660

Chapter DOI: 10.1128/9781555815660.ch37

MyBook is a cheap paperback edition of the original book and will be sold at uniform, low price.

Preview this chapter:

Molecular Comparison of Isolates from a Recurring Outbreak of Legionnaires’ Disease Spanning 22 Years, Page 1 of 2

< Previous page | Next page > /docserver/preview/fulltext/10.1128/9781555815660/9781555813901_Chap37-1.gif /docserver/preview/fulltext/10.1128/9781555815660/9781555813901_Chap37-2.gif

Abstract:

During outbreak investigations molecular analytical methods are necessary to discriminate among clinical isolates and to establish a link with environmental isolates. In addition to monoclonal antibodies used as an initial subtyping method, other methods employed have been pulse-field gel electrophoresis, amplified fragment length polymorphosim (AFLP), arbitrarily primed PCR, multilocus variable-number tandem repeat, and sequence-based typing (SBT). In the present study, the authors used SBT to study the relationships among clinical and environmental isolates of Legionella pneumophila isolated from the same hotel during a 22-year period. Water samples were collected from the hotel and cultured using standard procedures for the recovery of Legionella isolates from environmental sources. To determine the discriminatory power of the SBT procedure, eight isolates that share the same monoclonal antibody (MAb) subtype were included. These isolates represent four enzyme types based on previous studies using multilocus enzyme electrophoresis (MLEE). All isolates were tested with a panel of MAbs to determine their subtype. Isolates were compared by AFLP. Patient isolates and representative environmental isolates from both outbreaks were analyzed by a sequence-based typing scheme.

Citation: F. Benson R, E. Lucas C, W. Brown E, D. Cowgill K, S. Fields B. 2006. Molecular Comparison of Isolates from a Recurring Outbreak of Legionnaires’ Disease Spanning 22 Years, p 139-142. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), Legionella. ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch37

Highlighted Text: Show | Hide

Full text loading...

Figures

Molecular Comparison of Isolates from a Recurring Outbreak of Legionnaires’ Disease Spanning 22 Years

[com.pub2web.rdf.cci.facet.ContentItem[id=http://asm.metastore.ingenta.com/content/author/10.1128/9781555815660.ch37-1,webId=/content/author/10.1128/9781555815660.ch37-1,properties={foaf_givenname=Robert, foaf_name=Robert F. Benson, foaf_surname=F. Benson, pub_isAffiliatedWith=[com.pub2web.rdf.cci.facet.ContentItem[id=http://asm.metastore.ingenta.com/content/affiliation/10.1128/9781555815660.ch37-aff1]]}], com.pub2web.rdf.cci.facet.ContentItem[id=http://asm.metastore.ingenta.com/content/author/10.1128/9781555815660.ch37-2,webId=/content/author/10.1128/9781555815660.ch37-2,properties={foaf_givenname=Claressa, foaf_name=Claressa E. Lucas, foaf_surname=E. Lucas, pub_isAffiliatedWith=[com.pub2web.rdf.cci.facet.ContentItem[id=http://asm.metastore.ingenta.com/content/affiliation/10.1128/9781555815660.ch37-aff2]]}], com.pub2web.rdf.cci.facet.ContentItem[id=http://asm.metastore.ingenta.com/content/author/10.1128/9781555815660.ch37-3,webId=/content/author/10.1128/9781555815660.ch37-3,properties={foaf_givenname=Ellen, foaf_name=Ellen W. Brown, foaf_surname=W. Brown, pub_isAffiliatedWith=[com.pub2web.rdf.cci.facet.ContentItem[id=http://asm.metastore.ingenta.com/content/affiliation/10.1128/9781555815660.ch37-aff3]]}], com.pub2web.rdf.cci.facet.ContentItem[id=http://asm.metastore.ingenta.com/content/author/10.1128/9781555815660.ch37-4,webId=/content/author/10.1128/9781555815660.ch37-4,properties={foaf_givenname=Karen, foaf_name=Karen D. Cowgill, foaf_surname=D. Cowgill, pub_isAffiliatedWith=[com.pub2web.rdf.cci.facet.ContentItem[id=http://asm.metastore.ingenta.com/content/affiliation/10.1128/9781555815660.ch37-aff4]]}], com.pub2web.rdf.cci.facet.ContentItem[id=http://asm.metastore.ingenta.com/content/author/10.1128/9781555815660.ch37-5,webId=/content/author/10.1128/9781555815660.ch37-5,properties={foaf_givenname=Barry, foaf_name=Barry S. Fields, foaf_surname=S. Fields, pub_isAffiliatedWith=[com.pub2web.rdf.cci.facet.ContentItem[id=http://asm.metastore.ingenta.com/content/affiliation/10.1128/9781555815660.ch37-aff5]]}]]

/content/10.1128/9781555815660.ch37.ch37fig01

FIGURE 1

Genetic relatedness of St. Croix patient isolates from 1981 and 2003 and reference Legionella pneumophila serogroup 1 MAb 1,2,3 pattern.

10.1128/9781555815660/f0140-01_thmb.gif

10.1128/9781555815660/f0140-01.gif

FIGURE 1

Genetic relatedness of St. Croix patient isolates from 1981 and 2003 and reference Legionella pneumophila serogroup 1 MAb 1,2,3 pattern.

References

/content/book/10.1128/9781555815660.ch37

1. Aurell, H.,, J. Etienne,, F. Forey,, M. Reyrolle,, P. Girardo,, P. Farge,, B. Decludt,, C. Campese,, F. Vandenesch, and, S. Jarraud. 2003. Legionella pneumophila serogroup 1 strain Paris: endemic distribution throughout France. J. Clin. Microbiol. 41: 3320– 3322.

2. Aurell, H.,, P. Farge,, H. Meugnier,, M. Gouy,, F. Foey,, G. Lina,, F. Vandenesch,, J. Etienne, and, S. Jarraud. 2005. Clinical and environmental isolates of Legionella pneumophila serogroup 1 cannot be distinguished by sequence analysis of two surface protein genes and three housekeeping genes. Appl. Environ. Microbiol. 71: 282– 289.

3. Benson, R. F.,, B. S. Fields,, A. Benin,, H. Craddock, and, R. E. Besser. 2002. Application of amplified fragment length polymorphism analysis to subtyping of Legionella pneumophila serogroup 6, p. 243– 247. In R. Marre,, Y. Abu Kwaik,, C. Bartlett,, N. P. Cianciotto,, B. S. Fields,, M. Frosch,, J. Hacker,, P. C. Luck (ed.). Legionella. ASM Press, Washington, D.C.

4. Darelid, J.,, S. Bernander,, K. Jacobson, and, S. Lofgren. 2004. The presence of a specific genotype of Legionella pneumophila serogroup 1 in a hospital and municipal water distribution system over a 12-year period. Scand. J. Infect. Dis. 36: 417– 423.

5. Gaia, V.,, N. K. Fry,, B. Afshar,, P. C. Luck, et al. 2005. Consensus sequence-based scheme for epidemiological typing of clinical and environmental isolates of Legionella pneumophila. J. Clin. Microbiol. 43: 2047– 2052.

6. Kumar, S.,, K. Tamura, and, M. Nei. 2004. MEGA3: Integrated software for molecular evolutionary genetics analysis and sequence alignment. Briefings Bioinformatics 5: 150– 163.

7. Pruckler, J. M.,, L. A. Mermel,, R. F. Benson,, C. Giorgio,, P. K. Cassiday,, R. F. Breiman,, C. G. Whitney, and, B. S. Fields. 1995. Comparison of Legionella pneumophila isolates by arbitrarily primed PCR and pulsed-field gel electrophoresis: analysis from seven epidemic investigations. J. Clin. Microbiol. 33: 2872– 2875.

8. Rangel-Frausto, M. S.,, P. Rhomberg,, R. J. Hollis,, M. A. Pfaller,, R. P. Wenzel,, C. M. Helms, and, L. A. Herwaldt. 1999. Persistence of Legionella pneumophila in a hospital’s water system: a 13-year survey. Infect. Control. Hosp. Epidemiol. 20: 793– 797.

9. Selander, R. K.,, R. M. McKinney,, T. S. Whittam, W. F. Bibb,, D. J. Brenner,, F. S. Nolte, and, P. E. Pattison. 1985. Genetic structure of populations of Legionella pneumophila J. Bacteriol. 163: 1021– 1037.

Tables

Molecular Comparison of Isolates from a Recurring Outbreak of Legionnaires’ Disease Spanning 22 Years

/content/10.1128/9781555815660.ch37.ch37tab01

TABLE 1

SBT for isolates obtained from St. Croix, U.S.Virgin Islands during 1981 and 2003 epidemic investigations and reference strains

TABLE 1

SBT for isolates obtained from St. Croix, U.S.Virgin Islands during 1981 and 2003 epidemic investigations and reference strains

Press Catalog

View Latest ASM Press Catalog

Tools

Add to My Favorites
You must be logged in to use this functionality

Export citations
- BibT_EX
- Endnote
- Zotero
- Plain Text
- RefWorks
- Mendeley
Recommend to Library

These fields are mandatory:
*

Librarian details Name: *

Email: *

Your details Name: *

Email: *

Department: *

Why are you recommending this title?

Select reason:
I need to refer to this publication frequently

This publication is an essential resource for my studies/research

I'll refer my students to this publication

I'm an author/editor/contributor to this publication

I'm a member of the publication's editorial board

Other:

Legionella" />
eventtype:PERSONALISATION;jsessionid:phpbiAOf2u3Caz2Cl9T2DL-E.asmlive-10-241-2-96;itemid:http://asm.metastore.ingenta.com/content/book/10.1128/9781555815660.ch37;timestamp:1619180094079

Invalid site public key

Invalid site private key

Invalid request cookie

Incorrect. Try again.

Unabled to verify parameters

Could not contact recaptcha for validation

I am not a robot *

1160694123

Legionella — Recommend this title to your library

Thank you
Your recommendation has been sent to your librarian.
Request Permissions

Access Key

Free content
Open access content
Subscribed content