Chapter 38 : Sequence-Based Typing of as an Aid in Investigation of Hospital-Acquired Legionnaires’ Disease

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Sequence-Based Typing of as an Aid in Investigation of Hospital-Acquired Legionnaires’ Disease, Page 1 of 2

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The sequence-based typing (SBT) method was used to determine the allelic profiles of three sporadic clinical isolates as well as seven environmental isolates of serogroup 6, isolated at the Bacteriology Laboratory Unit of the Karolinska University Hospital in 2004. The SBT may prove to be an effective aid for the epidemiological investigation of nosocomial Legionnaires’ disease (LD). species, especially is a well-known cause of nosocomially acquired pneumonia, occurring frequently in immunocompromised subjects. The SBT method was used in a study to determine the allelic profiles of three serogroup 6 clinical isolates from suspected cases of nosocomial LD as well as seven environmental serogroup 6 isolates collected from drinking water sources in the vicinity of the patients being investigated. The aim of this study was to correlate the nosocomial infections to the environmental strain in the water systems of the hospital. Interestingly, the four control isolates collected from other hospitals in Sweden shared the same SBT profile, a finding that can be explained by the possible limited genomic heterogeneity within serogroup 6 compared to serogroup 1. Further typing of a larger number of serogroup 6 isolates is needed to validate this theory.

Citation: Fendukly F, Bernander S. 2006. Sequence-Based Typing of as an Aid in Investigation of Hospital-Acquired Legionnaires’ Disease, p 143-145. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch38
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