Chapter 84 : Bringing the Genome of to Life: the Transcriptional Program during Infection of

MyBook is a cheap paperback edition of the original book and will be sold at uniform, low price.

Preview this chapter:
Zoom in

Bringing the Genome of to Life: the Transcriptional Program during Infection of , Page 1 of 2

| /docserver/preview/fulltext/10.1128/9781555815660/9781555813901_Chap84-1.gif /docserver/preview/fulltext/10.1128/9781555815660/9781555813901_Chap84-2.gif


A whole-genome microarray was constructed which contained 3,823 gene-specific oligonucleotides representing every gene predicted in the strain Paris genome plus each loci specific to strains Lens (302 genes) and Philadelphia. In this short article, some results of the in vivo transcriptional program of the three strains during their life cycles in the natural eukaryotic host will be presented. A biphasic life cycle is clearly evident from the transcriptional data, with sets of replicative phase (RP) genes upregulated at the earlier time point and transmissive phase (TP) genes upregulated at the later time points. The global transcriptional programs of all three strains were very similar, indicating that common regulatory mechanisms govern their life cycles. When switching from a fast-growing bacterium to a motile, highly infectious microbe, the pathogen fundamentally alters its transcriptional program. Among the most significantly upregulated genes in the TP, the authors identified four two-component systems, six LysR-family members, four additional transcriptional regulators [CpxR, PaiA-, MoxR and TetR-like], and two integration host factors. The findings provide a valuable resource and conceptual framework to increase knowledge of the life cycle and the host functions exploited during infection of amoebae and macrophages.

Citation: Brüggemann H, Hagman A, Sismeiro O, Dillies M, Heuner K, Steinert M, Gouyette C, Coppée J, Buchrieser C. 2006. Bringing the Genome of to Life: the Transcriptional Program during Infection of , p 347-350. In Cianciotto N, Kwaik Y, Edelstein P, Fields B, Geary D, Harrison T, Joseph C, Ratcliff R, Stout J, Swanson M (ed), . ASM Press, Washington, DC. doi: 10.1128/9781555815660.ch84
Highlighted Text: Show | Hide
Loading full text...

Full text loading...


1. Cazalet, C.,, C. Rusniok,, H. Bruggemann,, N. Zidane,, A. Magnier,, L. Ma,, M. Tichit,, S. Jarraud,, C. Bouchier,, F. Vandenesch,, F. Kunst,, J. Etienne,, P. Glaser, and, C. Buchrieser. 2004. Evidence in the Legionella pneumophila genome for exploitation of host cell functions and high genome plasticity. Nat. Genet. 36: 11651173.
2. Chen, J.,, K. S. de Felipe,, M. Clarke,, H. Lu,, O. R. Anderson,, G. Segal, and, H. A. Shuman. 2004. Legionella effectors that promote nonlytic release from protozoa. Science 303: 13581361.
3. Chien, M.,, I. Morozova,, S. Shi,, H. Sheng,, J. Chen,, S. M. Gomez,, G. Asamani,, K. Hill,, J. Nuara,, M. Feder,, J. Rineer,, J. J. Greenberg,, V. Steshenko,, S. H. Park,, B. Zhao,, E. Teplits-kaya,, J. R. Edwards,, S. Pampou,, A. Georg-hiou,, I. C. Chou,, W. Iannuccilli,, M. E. Ulz,, D. H. Kim,, A. Geringer-Sameth,, C. Golds-berry,, P. Morozov,, S. G. Fischer,, G. Segal,, X. Qu,, A. Rzhetsky,, P. Zhang,, E. Cayanis,, P. J. De Jong,, J. Ju,, S. Kalachikov,, H. A. Shuman, and, J. J. Russo. 2004. The genomic sequence of the accidental pathogen Legionella pneumophila. Science 305: 19661968.
4. Cirillo, S. L.,, J. Lum, and, J. D. Cirillo. 2000. Identification of novel loci involved in entry by Legionella pneumophila. Microbiology 146: 13451359.
5. Heuner, K., and, M. Steinert. 2003. The flagel-lum of Legionella pneumophila and its link to the expression of the virulent phenotype. Int. J. Med. Microbiol. 293: 133143.
6. Luo, Z. Q., and, R. R. Isberg. 2004. Multiple substrates of the Legionella pneumophila Dot/Icm system identified by interbacterial protein transfer. Proc. Natl. Acad. Sci. USA 101: 841846.
7. Molofsky, A. B., and, M. S. Swanson. 2004. Differentiate to thrive: lessons from the Legionella pneumophila life cycle. Mol. Microbiol. Online publication date: 4-Jun-2004 .
8. Nagai, H., and, C. R. Roy. 2003. Show me the substrates: modulation of host cell function by type IV secretion systems. Cell. Microbiol. 5: 373383.
9. Romling, U.,, M. Gomelsky, and, M. Y. Galperin. 2005. C-di-GMP: the dawning of a novel bacterial signalling system. Mol. Microbiol. 57: 629639.
10. Shohdy, N.,, J. A. Efe,, S. D. Emr, and, H. A. Shuman. 2005. Pathogen effector protein screening in yeast identifies Legionella factors that interfere with membrane trafficking. Proc. Natl. Acad. Sci. USA 102: 48664871.

This is a required field
Please enter a valid email address
Please check the format of the address you have entered.
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error