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16 The Genomes of and

Authors: Catherine M. Ronning1, William C. Nierman2
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Affiliations: 1: J. Craig Venter Institute, 9712 Medical Center Dr., Rockville, MD 20850; 2: J. Craig Venter Institute, 9712 Medical Center Dr., Rockville, MD 20850
Content Type: Monograph
Publication Year: 2008
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Abstract:

This chapter discusses and compares the genomic sequences of DK1622 (hereafter referred to as DK1622 or strain DK1622) and DW4/3-1 (hereafter referred to as DW4/3-1 or strain DW4/3-1), two related aerobic, fruiting-body-forming myxobacteria. The structure and complexity of the fruiting body are the primary characteristics distinguishing it from DK1622, as well as the production of the signaling pheromone stigmolone, which may be analogous to the DK1622 quorum-sensing A-signal. Having the genome sequence of both organisms will greatly facilitate research into these and other myxobacteria-specific phenotypic traits. The DK1622 genome, initially sequenced to 4.5 coverage by Monsanto, was completed at The Institute for Genomic Research by additional random Sequencing. Both auto- and manual annotation were performed on the closed genome. Unlike most other prokaryotes, the myxobacteria exhibit social behavior and multicellular development. Such complex behavior is correlated with their large genomes. Gliding motility has also been extensively studied in DK1622, which, rather than swimming, hunts by gliding over the soil surface as a coordinated aggregation of thousands of cells, an essential element of its complex lifestyle. Myxobacteria feed by lysing cells of other bacteria and yeasts. Bioactive compounds synthesized and secreted through secondary metabolite biosynthetic gene clusters may aid predation and inhibit competition. The control of development in and is likely to be very similar based on the presence of DW4/3-1 orthologs to the genes controlling development in strain DK1622.

Citation: Ronning C, Nierman W. 2008. 16 The Genomes of and , p 285-298. In Whitworth D (ed), Myxobacteria. ASM Press, Washington, DC. doi: 10.1128/9781555815677.ch16
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Tables

16 The Genomes of Myxococcus xanthus and Stigmatella aurantiaca
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Citation: Ronning C, Nierman W. 2008. 16 The Genomes of and , p 285-298. In Whitworth D (ed), Myxobacteria. ASM Press, Washington, DC. doi: 10.1128/9781555815677.ch16
/content/10.1128/9781555815677.ch16.ch16tab01
Table 1
Selected features of the genomes of DK1622 and DW4/3-1
Generic image for table
Table 1

Selected features of the genomes of DK1622 and DW4/3-1

Citation: Ronning C, Nierman W. 2008. 16 The Genomes of and , p 285-298. In Whitworth D (ed), Myxobacteria. ASM Press, Washington, DC. doi: 10.1128/9781555815677.ch16
/content/10.1128/9781555815677.ch16.ch16tab02
Table 2
Putative chemotaxis gene clusters identified in DK1622 and their DW4/3-1 orthologs, identified by the presence of genesa
Generic image for table
Table 2

Putative chemotaxis gene clusters identified in DK1622 and their DW4/3-1 orthologs, identified by the presence of genesa

Citation: Ronning C, Nierman W. 2008. 16 The Genomes of and , p 285-298. In Whitworth D (ed), Myxobacteria. ASM Press, Washington, DC. doi: 10.1128/9781555815677.ch16
/content/10.1128/9781555815677.ch16.ch16tab03
Table 3
Secondary metabolite biosynthetic gene clusters in and putative orthology (if present) in DW4/3-1
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Table 3

Secondary metabolite biosynthetic gene clusters in and putative orthology (if present) in DW4/3-1

Citation: Ronning C, Nierman W. 2008. 16 The Genomes of and , p 285-298. In Whitworth D (ed), Myxobacteria. ASM Press, Washington, DC. doi: 10.1128/9781555815677.ch16
/content/10.1128/9781555815677.ch16.ch16tab04
Table 4
Secondary metabolite biosynthetic gene clusters in DW4/3-1 and putative orthology (if present) in
Generic image for table
Table 4

Secondary metabolite biosynthetic gene clusters in DW4/3-1 and putative orthology (if present) in

Citation: Ronning C, Nierman W. 2008. 16 The Genomes of and , p 285-298. In Whitworth D (ed), Myxobacteria. ASM Press, Washington, DC. doi: 10.1128/9781555815677.ch16
/content/10.1128/9781555815677.ch16.ch16tab05
Table 5
Gas vesicle genes identified in DK1622 and putative orthologs in DW4/3-1a
Generic image for table
Table 5

Gas vesicle genes identified in DK1622 and putative orthologs in DW4/3-1a

Citation: Ronning C, Nierman W. 2008. 16 The Genomes of and , p 285-298. In Whitworth D (ed), Myxobacteria. ASM Press, Washington, DC. doi: 10.1128/9781555815677.ch16

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