Chapter 29 : Conclusions

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The limitation of agar plates to recover microorganisms from environmental samples was realized over a century ago by Winogradsky. Indeed, the great majority of the marine microbiota is elusive to growth on conventional laboratory media, probably due to the artificially high nutrient load of commercial media and incompatibility of the media with the environmental conditions. It is clear that there is still a widespread reliance on thiosulfate citrate bile salt sucrose (TCBS) agar for the recovery of vibrios, despite its formulation being specifically for cholera. Recent studies have shown that quantitative data obtained using TCBS parallel the molecular counts by quantitative PCR. Biofilm formation is an important feature in the ecology (and pathogenesis) of vibrios. The development of biofilms is under orchestrated and complex genetic control involving several independent loci. Phenotyping techniques will probably give place to molecular tools for the needed screening of massive numbers of isolates. Multilocus sequence analysis (MLSA) and whole-genome sequence data will also allow vibrio taxonomists to address questions concerning the species definition and concept, a highly debatable issue in the current prokaryotic taxonomy. The usefulness of genome-based approaches to taxonomy, using amino acid identity of MLSA data, may offer insights on vibrio systematics. Lipopolysaccharide and peptidoglycan monomer (PGM) molecules normally trigger antimicrobial responses by the innate immune system of animals, but in the specific case of and , the host seems to tolerate the benign infection.

Citation: Thompson F, Austin B, Swings J. 2006. Conclusions, p 409-. In Thompson F, Austin B, Swings J (ed), The Biology of Vibrios. ASM Press, Washington, DC. doi: 10.1128/9781555815714.ch29
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