Chapter 5 : Animal Models of Pneumococcal Colonization

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This chapter describes existing animal models of colonization and reviews what these various models have taught us about pneumococcal carriage. The most widely studied model of pneumococcal colonization is the mouse model. Infant rat models of pneumococcal colonization have also been described in several studies. A buccal mucosa model was also developed to evaluate the impact of ambient temperatures on pneumococcal colonization. The major limitation of chinchilla model is the relative lack of chinchilla reagents, as well as the expense. Animal models have provided information about the contribution of specific bacterial components to colonization, the first step in the pathogenesis of all pneumococcal disease. Different laboratories have used different mouse strains, which often vary in susceptibility to pneumococcal colonization and disease. Murine models of pneumococcal colonization have helped enhance the understanding of acquired immunity to pneumococcal colonization. The infant rat model of intralitter spread was used to evaluate the impact of systemic anticapsular antibodies in the prevention of pneumococcal colonization. The administration of bacterial polysaccharide immune globulin (from hyperimmune sera obtained from adults immunized with pneumococcal, type b, and meningococcal pure-polysaccharide vaccines) to infant rats reduced the likelihood of pneumococcal colonization by about 50%. The development of purified-protein vaccines or whole-cell vaccines, however, faces several challenges, including the inherent difficulty of studying colonization in animals that normally do not carry in their respiratory trees.

Citation: Malley R, Weiser J. 2008. Animal Models of Pneumococcal Colonization, p 59-66. In Siber G, Klugman K, Mäkelä P (ed), Pneumococcal Vaccines. ASM Press, Washington, DC. doi: 10.1128/9781555815820.ch5
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