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Chapter 18 : Effect of Defensins on Susceptibility to Infection at the Mucosal Surface

Authors: Janet M. Guthmiller1, Sophie Joly2
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Affiliations: 1: Department of Periodontics and Dows Institute for Dental Research, College of Dentistry, University of Iowa, Iowa City, IA 52242-1001; 2: Dows Institute of Dental Research, College of Dentistry, University of lowa, lowa City, IA 52242-1001
Content Type: Monograph
Publication Year: 2007

Category: Bacterial Pathogenesis

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Abstract:

Periodontal diseases are among the most common diseases of the oral cavity. Periodontal disease is the major cause of tooth loss in adults, and recently there has been increasing interest in the relationship of periodontal disease to important systemic diseases, such as cardiovascular disease and complications in pregnancy. Despite the importance of infection and colonization by bacteria and yeast in periodontal and candidal infections, the immune status of the host and the effectiveness of the host response are key determinants of disease susceptibility. The regulation of β-defensins by various mediators and their expression in oral tissues, salivary glands, and secretions suggest that β-defensins are in a prime position to defend epithelial cells and mucosa from infection and respond to inflammation in the periodontium. The complexity of multiple regulatory pathways involved in β-defensin expression and their physicochemical properties and structures supports their pluripotential functions. β-Defensin expression also appears to be regulated during inflammation in vivo. Unlike the results of in vitro studies demonstrating upregulation by inflammatory stimulants, such upregulation does not always occur in vivo. Understanding the basis for differential expression of β-defensins in the oral cavity will assist us in defining individuals at risk for periodontal diseases and infections.

Citation: Guthmiller J, Joly S. 2007. Effect of Defensins on Susceptibility to Infection at the Mucosal Surface, p 265-280. In Brogden K, Minion F, Cornick N, Stanton T, Zhang Q, Nolan L, Wannemuehler M (ed), Virulence Mechanisms of Bacterial Pathogens, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815851.ch18

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Effect of Defensins on Susceptibility to Infection at the Mucosal Surface
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Citation: Guthmiller J, Joly S. 2007. Effect of Defensins on Susceptibility to Infection at the Mucosal Surface, p 265-280. In Brogden K, Minion F, Cornick N, Stanton T, Zhang Q, Nolan L, Wannemuehler M (ed), Virulence Mechanisms of Bacterial Pathogens, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815851.ch18
/content/10.1128/9781555815851.ch18.ch18fig01
FIGURE 1
Inducibility of HBD-1, HBD-2, and HBD-3 mRNA expression by cytokines (subject 316). mRNA expression stimulated by various cytokines and combinations of cytokines is seen for HBD-1 (panel A) using reverse transcription-PCR ( ) and real-time PCR ( ) and real-time PCR analysis for HBD-2 (panel B) and HBD-3 (panel C). The results from the cell line presented are typical of those seen for three additional subjects in whose cells the cytokines were tested. Each of the defensins demonstrates significant upregulation by individual cytokines and synergistic expression with select combinations of cytokines. Molecular sizes in base pairs are presented on each side of the gel in panel A. The GAPDH (glyceraldehyde-3-phosphate dehydrogenase) gene (421 bp) was used as a control housekeeping gene. Numbers in parentheses represent the amount (nanograms) used for the stimulations when more than one concentration was used for a particular cytokine. Asterisks indicate data that were significantly ( < 0.05) different from the control (medium alone). Error bars represent the standard error of the mean of triplicate measures. C+, HBD-1 cDNA-positive control. Reprinted from reference with permission.
10.1128/9781555815851/f0268-01_thmb.gif
10.1128/9781555815851/f0268-01.gif
Image of FIGURE 1
FIGURE 1

Inducibility of HBD-1, HBD-2, and HBD-3 mRNA expression by cytokines (subject 316). mRNA expression stimulated by various cytokines and combinations of cytokines is seen for HBD-1 (panel A) using reverse transcription-PCR ( ) and real-time PCR ( ) and real-time PCR analysis for HBD-2 (panel B) and HBD-3 (panel C). The results from the cell line presented are typical of those seen for three additional subjects in whose cells the cytokines were tested. Each of the defensins demonstrates significant upregulation by individual cytokines and synergistic expression with select combinations of cytokines. Molecular sizes in base pairs are presented on each side of the gel in panel A. The GAPDH (glyceraldehyde-3-phosphate dehydrogenase) gene (421 bp) was used as a control housekeeping gene. Numbers in parentheses represent the amount (nanograms) used for the stimulations when more than one concentration was used for a particular cytokine. Asterisks indicate data that were significantly ( < 0.05) different from the control (medium alone). Error bars represent the standard error of the mean of triplicate measures. C+, HBD-1 cDNA-positive control. Reprinted from reference with permission.

Citation: Guthmiller J, Joly S. 2007. Effect of Defensins on Susceptibility to Infection at the Mucosal Surface, p 265-280. In Brogden K, Minion F, Cornick N, Stanton T, Zhang Q, Nolan L, Wannemuehler M (ed), Virulence Mechanisms of Bacterial Pathogens, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815851.ch18
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/content/10.1128/9781555815851.ch18.ch18fig02
FIGURE 2
Susceptibility of aerobic and anaerobic oral bacteria to HBD-2 and HBD-3. MICs, obtained from radial diffusion assays, of HBD-2 (A) and HBD-3 (B) for (), (), (), (), (), (), (), and () are shown. Values are means and standard deviations of triplicate assays.
10.1128/9781555815851/f0271-01_thmb.gif
10.1128/9781555815851/f0271-01.gif
Image of FIGURE 2
FIGURE 2

Susceptibility of aerobic and anaerobic oral bacteria to HBD-2 and HBD-3. MICs, obtained from radial diffusion assays, of HBD-2 (A) and HBD-3 (B) for (), (), (), (), (), (), (), and () are shown. Values are means and standard deviations of triplicate assays.

Citation: Guthmiller J, Joly S. 2007. Effect of Defensins on Susceptibility to Infection at the Mucosal Surface, p 265-280. In Brogden K, Minion F, Cornick N, Stanton T, Zhang Q, Nolan L, Wannemuehler M (ed), Virulence Mechanisms of Bacterial Pathogens, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815851.ch18
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References

/content/book/10.1128/9781555815851.ch18
1. Ali, R. S.,, A. Falconer,, M. Ikram,, C. E. Bissett,, R. Cerio, and, A. G. Quinn. 2001. Expression of the peptide antibiotics human beta defensin-1 and human beta defensin-2 in normal human skin. J. Investig. Dermatol. 117: 106– 111.
2. Baroni, A.,, M. Orlando,, G. Donnarumma,, P. Farro,, M. R. Iovene,, M. A. Tufano, and, E. Buommino. 2006. Toll-like receptor 2 (TLR2) mediates intracellular signalling in human keratinocytes in response to Malassezia furfur. Arch. Dermatol. Res. 297: 280– 288.
3. Becker, M. N.,, G. Diamond,, M. W. Verghese, and, S. H. Randell. 2000. CD14-dependent lipopolysaccharide-induced beta-defensin-2 expression in human tracheobronchial epithelium. J. Biol. Chem. 275: 29731– 29736.
4. Beisswenger, C.,, K. Kandler,, C. Hess,, H. Garn,, K. Felgentreff,, M. Wegmann,, H. Renz,, C. Vogelmeier, and, R. Bals. 2006. Allergic air-way inflammation inhibits pulmonary antibacterial host defense. J. Immunol. 177: 1833– 1837.
5. Bianchi, M.,, C. Meng, and, L. B. Ivashkiv. 2000. Inhibition of IL-2-induced Jak-STAT signaling by glucocorticoids. Proc. Natl. Acad. Sci. USA 97: 9573– 9578.
6. Birchler, T.,, R. Seibl,, K. Buchner,, S. Loeliger,, R. Seger,, J. P. Hossle,, A. Aguzzi, and, R. P. Lauener. 2001. Human Toll-like receptor 2 mediates induction of the antimicrobial peptide human beta-defensin 2 in response to bacterial lipoprotein. Eur. J. Immunol. 31: 3131– 3137.
7. Bissell, J.,, S. Joly,, G. K. Johnson,, C. C. Organ,, D. Dawson,, P. B. McCray, Jr., and, J. M. Guth-miller. 2004. Expression of beta-defensins in gingival health and in periodontal disease. J. Oral Pathol. Med. 33: 278– 285.
8. Boggess, K. A.,, J. D. Beck,, A. P. Murtha,, K. Moss, and, S. Offenbacher. 2006. Maternal periodontal disease in early pregnancy and risk for a small-for-gestational-age infant. Am. J. Obstet. Gynecol. 194: 1316– 1322.
9. Boniotto, M.,, M. H. Hazbon,, W. J. Jordan,, G. P. Lennon,, J. Eskdale,, D. Alland, and, G. Gallagher. 2004. Novel hairpin-shaped primer assay to study the association of the −44 single-nucleotide polymorphism of the DEFB1 gene with early-onset periodontal disease. Clin. Diagn. Lab. Immunol. 11: 766– 769.
10. Boniotto, M.,, M. Ventura,, J. Eskdale,, S. Crovella, and, G. Gallagher. 2004. Evidence for duplication of the human defensin gene DEFB4 in chromosomal region 8p22-23 and implications for the analysis of SNP allele distribution. Genet. Test. 8: 325– 327.
11. Boughan, P. K.,, R. H. Argent,, M. Body-Malapel,, J. H. Park,, K. E. Ewings,, A. G. Bowie,, S. J. Ong,, S. J. Cook,, O. E. Sorensen,, B. A. Manzo,, N. Inohara,, N. J. Klein,, G. Nunez,, J. C. Atherton, and, M. Bajaj-Elliott. 2006. Nucleotide-binding oligomerization domain-1 and epidermal growth factor receptor: critical regulators of beta-defensins during Helicobacter pylori infection. J. Biol. Chem. 281: 11637– 11648.
12. Braida, L.,, M. Boniotto,, A. Pontillo,, P. A. Tovo,, A. Amoroso, and, S. Crovella. 2004. A single-nucleotide polymorphism in the human beta-defensin 1 gene is associated with HIV-1 infection in Italian children. AIDS 18: 1598– 1600.
13. Brissette, C. A.,, L. G. Simonson, and, S. A. Lukehart. 2004. Resistance to human beta-defensins is common among oral treponemes. Oral Microbiol. Immunol. 19: 403– 407.
14. Brogden, K. A.,, M. Heidari,, R. E. Sacco,, D. Palmquist,, J. M. Guthmiller,, G. K. Johnson,, H. P. Jia,, B. F. Tack, and, P. B. McCray. 2003. Defensin-induced adaptive immunity in mice and its potential in preventing periodontal disease. Oral Microbiol. Immunol. 18: 95– 99.
15. Challacombe, S. J. 1994. Immunologic aspects of oral candidiasis. Oral Surg. Oral Med. Oral Pathol. 78: 202– 210.
16. Chong, K. T.,, L. Xiang,, X. Wang,, E. L. Jun,, L. F. Xi, and, J. M. Schweinfurth. 2006. High level expression of human epithelial beta-defensins (hBD-1, 2 and 3) in papillomavirus induced lesions. Virol. J. 3: 75.
17. Chung, W. O., and, B. A. Dale. 2004. Innate immune response of oral and foreskin keratinocytes: utilization of different signaling pathways by various bacterial species. Infect. Immun. 72: 352– 358.
18. Chung, W. O.,, S. R. Hansen,, D. Rao, and, B. A. Dale. 2004. Protease-activated receptor signaling increases epithelial antimicrobial peptide expression. J. Immunol. 173: 5165– 5170.
19. Cole, A. M. 2006. Innate host defense of human vaginal and cervical mucosae. Curr. Top. Microbiol. Immunol. 306: 199– 230.
20. Crovella, S.,, N. Antcheva,, I. Zelezetsky,, M. Boniotto,, S. Pacor,, M. V. Verga Falzacappa, and, A. Tossi. 2005. Primate beta-defensins structure, function and evolution. Curr. Protein Pept. Sci. 6: 7– 21.
21. Dale, B. A. 2002. Periodontal epithelium: a newly recognized role in health and disease. Periodontol. 2000 30: 70– 78.
22. Dale, B. A.,, J. R. Kimball,, S. Krisanaprakornkit,, F. Roberts,, M. Robinovitch,, R. O’Neal,, E. V. Valore,, T. Ganz,, G. M. Anderson, and, A. Weinberg. 2001. Localized antimicrobial peptide expression in human gingiva. J. Periodontal Res. 36: 285– 294.
23. Dale, B. A., and, S. Krisanaprakornkit. 2001. Defensin antimicrobial peptides in the oral cavity. J. Oral Pathol. Med. 30: 321– 327.
24. Dale, B. A.,, R. Tao,, J. R. Kimball, and, R. J. Jurevic. 2006. Oral antimicrobial peptides and biological control of caries. BMC Oral Health 6 ( Suppl. 1) : S13.
25. Darveau, R. P.,, A. Tanner, and, R. C. Page. 1997. The microbial challenge in periodontitis. Periodontol. 2000 14: 12– 32.
26. Diamond, D. L.,, J. R. Kimball,, S. Krisanaprakornkit,, T. Ganz, and, B. A. Dale. 2001. Detection of beta-defensins secreted by human oral epithelial cells. J. Immunol. Methods 256: 65– 76.
27. Diamond, G.,, D. E. Jones, and, C. L. Bevins. 1993. Airway epithelial cells are the site of expression of a mammalian antimicrobial peptide gene. Proc. Natl. Acad. Sci. USA 90: 4596– 4600.
28. Diamond, G.,, V. Kaiser,, J. Rhodes,, J. P. Russell, and, C. L. Bevins. 2000. Transcriptional regulation of beta-defensin gene expression in tracheal epithelial cells. Infect. Immun. 68: 113– 119.
29. Dowd, S. E., and, H. Ishizaki. 2006. Microarray based comparison of two Escherichia coli O157: H7 lineages. BMC Microbiol. 6: 30.
30. Duits, L. A.,, M. Rademaker,, B. Ravensbergen,, M. A. van Sterkenburg,, E. van Strijen,, P. S. Hiemstra, and, P. H. Nibbering. 2001. Inhibition of hBD-3, but not hBD-1 and hBD-2, mRNA expression by corticosteroids. Biochem. Biophys. Res. Commun. 280: 522– 525.
31. Dunsche, A.,, Y. Acil,, H. Dommisch,, R. Siebert,, J. M. Schroder, and, S. Jepsen. 2002. The novel human beta-defensin-3 is widely expressed in oral tissues. Eur. J. Oral Sci. 110: 121– 124.
32. Dunsche, A.,, Y. Acil,, R. Siebert,, J. Harder,, J. M. Schroder, and, S. Jepsen. 2001. Expression profile of human defensins and antimicrobial proteins in oral tissues. J. Oral Pathol. Med. 30: 154– 158.
33. Fellermann, K.,, D. E. Stange,, E. Schaeffeler,, H. Schmalzl,, J. Wehkamp,, C. L. Bevins,, W. Reinisch,, A. Teml,, M. Schwab,, P. Lichter,, B. Radlwimmer, and, E. F. Stange. 2006. A chromosome 8 gene-cluster polymorphism with low human beta-defensin 2 gene copy number predisposes to Crohn disease of the colon. Am. J. Hum. Genet. 79: 439– 448.
34. Fellermann, K.,, J. Wehkamp,, K. R. Herrlinger, and, E. F. Stange. 2003. Crohn’s disease: a defensin deficiency syndrome? Eur. J. Gastroenterol. Hepatol. 15: 627– 634.
35. Feng, Z.,, B. Jiang,, J. Chandra,, M. Ghannoum,, S. Nelson, and, A. Weinberg. 2005. Human beta-defensins: differential activity against candidal species and regulation by Candida albicans. J. Dent. Res. 84: 445– 450.
36. Fernie-King, B. A.,, D. J. Seilly, and, P. J. Lachmann. 2006. Inhibition of antimicrobial peptides by group A streptococci: SIC and DRS. Biochem. Soc. Trans. 34: 273– 275.
37. Feucht, E. C.,, C. L. DeSanti, and, A. Weinberg. 2003. Selective induction of human beta-defensin mRNAs by Actinobacillus actinomycetemcomitans in primary and immortalized oral epithelial cells. Oral Microbiol. Immunol. 18: 359– 363.
38. Fidel, P. L., Jr. 2002. Immunity to Candida. Oral Dis. 8( Suppl. 2) : 69– 75.
39. Frye, M.,, J. Bargon, and, R. Gropp. 2001. Expression of human beta-defensin-1 promotes differentiation of keratinocytes. J. Mol. Med. 79: 275– 282.
40. Ganz, T. 2003. Defensins: antimicrobial peptides of innate immunity. Nat. Rev. Immunol. 3: 710– 720.
41. Ganz, T., and, R. I. Lehrer. 1998. Antimicrobial peptides of vertebrates. Curr. Opin. Immunol. 10: 41– 44.
42. Ganz, T., and, R. I. Lehrer. 1995. Defensins. Pharmacol. Ther. 66: 191– 205.
43. Garcia, J. R.,, F. Jaumann,, S. Schulz,, A. Krause,, J. Rodriguez-Jimenez,, U. Forssmann,, K. Adermann,, E. Kluver,, C. Vogelmeier,, D. Becker,, R. Hedrich,, W. G. Forssmann, and, R. Bals. 2001. Identification of a novel, multi-functional beta-defensin (human beta-defensin 3) with specific antimicrobial activity. Its interaction with plasma membranes of Xenopus oocytes and the induction of macrophage chemoattraction. Cell Tissue Res. 306: 257– 264.
44. Garcia, J. R.,, A. Krause,, S. Schulz,, F. J. Rodriguez-Jimenez,, E. Kluver,, K. Adermann,, U. Forssmann,, A. Frimpong-Boateng,, R. Bals, and, W. G. Forssmann. 2001. Human beta-defensin 4: a novel inducible peptide with a specific salt-sensitive spectrum of antimicrobial activity. FASEB J. 15: 1819– 1821.
45. Genco, R.,, S. Offenbacher, and, J. Beck. 2002. Periodontal disease and cardiovascular disease: epidemiology and possible mechanisms. J. Am. Dent. Assoc. 133(Suppl) : 14S– 22S.
46. Goldman, M. J.,, G. M. Anderson,, E. D. Stolzenberg,, U. P. Kari,, M. Zasloff, and, J. M. Wilson. 1997. Human beta-defensin-1 is a salt-sensitive antibiotic in lung that is inactivated in cystic fibrosis. Cell 88: 553– 560.
47. Guthmiller, J. M.,, K. G. Vargas,, R. Srikantha,, L. L. Schomberg,, P. L. Weistroffer,, P. B. McCray, Jr., and, B. F. Tack. 2001. Susceptibilities of oral bacteria and yeast to mammalian cathelicidins. Antimicrob. Agents Chemother. 45: 3216– 3219.
48. Harder, J.,, J. Bartels,, E. Christophers, and, J. M. Schroder. 2001. Isolation and characterization of human beta-defensin-3, a novel human inducible peptide antibiotic. J. Biol. Chem. 276: 5707– 5713.
49. Harder, J.,, J. Bartels,, E. Christophers, and, J. M. Schroder. 1997. A peptide antibiotic from human skin. Nature 387: 861.
50. Harder, J.,, U. Meyer-Hoffert,, L. M. Teran,, L. Schwichtenberg,, J. Bartels,, S. Maune, and, J. M. Schroder. 2000. Mucoid Pseudomonas aeruginosa, TNF-alpha, and IL-1beta, but not IL-6, induce human beta-defensin-2 in respiratory epithelia. Am. J. Respir. Cell Mol. Biol. 22: 714– 721.
51. Harder, J.,, U. Meyer-Hoffert,, K. Wehkamp,, L. Schwichtenberg, and, J. M. Schroder. 2004. Differential gene induction of human beta-defensins (hBD-1, -2, -3, and -4) in keratinocytes is inhibited by retinoic acid. J. Investig. Dermatol. 123: 522– 529.
52. Hertz, C. J.,, Q. Wu,, E. M. Porter,, Y. J. Zhang,, K. H. Weismuller,, P. J. Godowski,, T. Ganz,, S. H. Randell, and, R. L. Modlin. 2003. Activation of Toll-like receptor 2 on human tracheobronchial epithelial cells induces the antimicrobial peptide human beta defensin-2. J. Immunol. 171: 6820– 6826.
53. Hollox, E. J.,, J. A. Armour, and, J. C. Barber. 2003. Extensive normal copy number variation of a beta-defensin antimicrobial-gene cluster. Am. J. Hum. Genet. 73: 591– 600.
54. Howell, M. D.,, M. Boguniewicz,, S. Pastore,, G. Girolomoni, and, D. Y. Leung. 2006. Mechanism of HBD-3 deficiency in atopic dermatitis. Clin. Immunol. 121: 332– 338.
55. Howell, M. D.,, N. Novak,, T. Bieber,, S. Pastore,, G. Girolomoni,, M. Boguniewicz,, J. Streib,, C. Wong,, R. L. Gallo, and, D. Y. Leung. 2005. Interleukin-10 downregulates anti-microbial peptide expression in atopic dermatitis. J. Investig. Dermatol. 125: 738– 745.
56. Hu, R. C.,, Y. J. Xu,, Z. X. Zhang,, W. Ni, and, S. X. Chen. 2004. Correlation of HDEFB1 polymorphism and susceptibility to chronic obstructive pulmonary disease in Chinese Han population. Chin. Med. J. 117: 1637– 1641.
57. Hughes, A. L. 1994. The evolution of functionally novel proteins after gene duplication. Proc. R. Soc. Lond. B 256: 119– 124.
58. Islam, D.,, L. Bandholtz,, J. Nilsson,, H. Wigzell,, B. Christensson,, B. Agerberth, and, G. Gudmundsson. 2001. Downregulation of bactericidal peptides in enteric infections: a novel immune escape mechanism with bacterial DNA as a potential regulator. Nat. Med. 7: 180– 185.
59. Jang, B. C.,, K. J. Lim,, J. H. Paik,, Y. K. Kwon,, S. W. Shin,, S. C. Kim,, T. Y. Jung,, T. K. Kwon,, J. W. Cho,, W. K. Baek,, S. P. Kim,, M. H. Suh, and, S. I. Suh. 2004. Up-regulation of human beta-defensin 2 by interleukin-1beta in A549 cells: involvement of PI3K, PKC, p38 MAPK, JNK, and NF-kappaB. Biochem. Biophys. Res. Commun. 320: 1026– 1033.
60. Jia, H. P.,, B. C. Schutte,, A. Schudy,, R. Linz-meier,, J. M. Guthmiller,, G. K. Johnson,, B. F. Tack,, J. P. Mitros,, A. Rosenthal,, T. Ganz, and, P. B. McCray, Jr. 2001. Discovery of new human beta-defensins using a genomics-based approach. Gene 263: 211– 218.
61. Joly, S.,, C. Maze,, P. B. McCray, Jr., and, J. M. Guthmiller. 2004. Human beta-defensins 2 and 3 demonstrate strain-selective activity against oral microorganisms. J. Clin. Microbiol. 42: 1024– 1029.
62. Joly, S.,, C. C. Organ,, G. K. Johnson,, P. B. McCray, and, J. M. Guthmiller. 2005. Correlation between beta-defensin expression and induction profiles in gingival keratinocytes. Mol. Immunol. 42: 1073– 1084.
63. Jurevic, R. J.,, M. Bai,, R. B. Chadwick,, T. C. White, and, B. A. Dale. 2003. Single-nucleotide polymorphisms (SNPs) in human beta-defensin 1: high-throughput SNP assays and association with Candida carriage in type I diabetics and nondiabetic controls. J. Clin. Microbiol. 41: 90– 96.
64. Jurevic, R. J.,, P. Chrisman,, L. Mancl,, R. Livingston, and, B. A. Dale. 2002. Single-nucleotide polymorphisms and haplotype analysis in beta-defensin genes in different ethnic populations. Genet. Test. 6: 261– 269.
65. Kao, C. Y.,, Y. Chen,, P. Thai,, S. Wachi,, F. Huang,, C. Kim,, R. W. Harper, and, R. Wu. 2004. IL-17 markedly up-regulates beta-defensin-2 expression in human airway epithelium via JAK and NF-kappaB signaling pathways. J. Immunol. 173: 3482– 3491.
66. Karabinis, A.,, C. Hill,, B. Leclercq,, C. Tancrede,, D. Baume, and, A. Andremont. 1988. Risk factors for candidemia in cancer patients: a case-control study. J. Clin. Microbiol. 26: 429– 432.
67. Klotman, M. E., and, T. L. Chang. 2006. Defensins in innate antiviral immunity. Nat. Rev. Immunol. 6: 447– 456.
68. Krisanaprakornkit, S.,, J. R. Kimball, and, B. A. Dale. 2002. Regulation of human beta-defensin-2 in gingival epithelial cells: the involvement of mitogen-activated protein kinase pathways, but not the NF-kappaB transcription factor family. J. Immunol. 168: 316– 324.
69. Krisanaprakornkit, S.,, J. R. Kimball,, A. Weinberg,, R. P. Darveau,, B. W. Bainbridge, and, B. A. Dale. 2000. Inducible expression of human beta-defensin 2 by Fusobacterium nucleatum in oral epithelial cells: multiple signaling pathways and role of commensal bacteria in innate immunity and the epithelial barrier. Infect. Immun. 68: 2907– 2915.
70. Krisanaprakornkit, S.,, A. Weinberg,, C. N. Perez, and, B. A. Dale. 1998. Expression of the peptide antibiotic human beta-defensin 1 in cultured gingival epithelial cells and gingival tissue. Infect. Immun. 66: 4222– 4228.
71. Lamkin, M. S., and, F. G. Oppenheim. 1993. Structural features of salivary function. Crit. Rev. Oral Biol. Med. 4: 251– 259.
72. Levy, H.,, B. A. Raby,, S. Lake,, K. G. Tantisira,, D. Kwiatkowski,, R. Lazarus,, E. K. Silverman,, B. Richter,, W. T. Klimecki,, D. Vercelli,, F. D. Martinez, and, S. T. Weiss. 2005. Association of defensin beta-1 gene polymorphisms with asthma. J. Allergy Clin. Immunol. 115: 252– 258.
73. Linzmeier, R.,, C. H. Ho,, B. V. Hoang, and, T. Ganz. 1999. A 450-kb contig of defensin genes on human chromosome 8p23. Gene 233: 205– 211.
74. Linzmeier, R. M., and, T. Ganz. 2006. Copy number polymorphisms are not a common feature of innate immune genes. Genomics 88: 122– 126.
75. Linzmeier, R. M., and, T. Ganz. 2005. Human defensin gene copy number polymorphisms: comprehensive analysis of independent variation in alpha- and beta-defensin regions at 8p22-p23. Genomics 86: 423– 430.
76. Liu, L.,, L. Wang,, H. P. Jia,, C. Zhao,, H. H. Heng,, B. C. Schutte,, P. B. McCray, Jr., and, T. Ganz. 1998. Structure and mapping of the human beta-defensin HBD-2 gene and its expression at sites of inflammation. Gene 222: 237– 244.
77. Lu, Q.,, J. A. Jayatilake,, L. P. Samaranayake, and, L. Jin. 2006. Hyphal invasion of Candida albicans inhibits the expression of human beta-defensins in experimental oral candidiasis. J. Investig. Dermatol. 126: 2049– 2056.
78. Lu, Q.,, L. Jin,, R. P. Darveau, and, L. P. Samaranayake. 2004. Expression of human beta-defensins-1 and -2 peptides in unresolved chronic periodontitis. J. Periodontal Res. 39: 221– 227.
79. MacRedmond, R.,, C. Greene,, C. C. Taggart,, N. McElvaney, and, S. O’Neill. 2005. Respiratory epithelial cells require Toll-like receptor 4 for induction of human beta-defensin 2 by lipopolysaccharide. Respir. Res. 6: 116.
80. Mathews, M.,, H. P. Jia,, J. M. Guthmiller,, G. Losh,, S. Graham,, G. K. Johnson,, B. F. Tack, and, P. B. McCray, Jr. 1999. Production of beta-defensin antimicrobial peptides by the oral mucosa and salivary glands. Infect. Immun. 67: 2740– 2745.
81. McDermott, A. M.,, R. L. Redfern,, B. Zhang,, Y. Pei,, L. Huang, and, R. J. Proske. 2003. Defensin expression by the cornea: multiple signalling pathways mediate IL-1beta stimulation of hBD-2 expression by human corneal epithelial cells. Investig. Ophthalmol. Vis. Sci. 44: 1859– 1865.
82. Menzies, B. E., and, A. Kenoyer. 2006. Signal transduction and nuclear responses in Staphylococcus aureus-induced expression of human β-defensin 3 in skin keratinocytes. Infect. Immun. 74: 6847– 6854.
83. Morrison, G.,, F. Kilanowski,, D. Davidson, and, J. Dorin. 2002. Characterization of the mouse beta defensin 1, Defb1, mutant mouse model. Infect. Immun. 70: 3053– 3060.
84. Moser, C.,, D. J. Weiner,, E. Lysenko,, R. Bals,, J. N. Weiser, and, J. M. Wilson. 2002. β-Defensin 1 contributes to pulmonary innate immunity in mice. Infect. Immun. 70: 3068– 3072.
85. Motzkus, D.,, S. Schulz-Maronde,, A. Heitland,, A. Schulz,, W. G. Forssmann,, M. Jubner, and, E. Maronde. 2006. The novel beta-defensin DEFB123 prevents lipopolysaccharide-mediated effects in vitro and in vivo. FASEB J. 20: 1701– 1702.
86. Niyonsaba, F.,, M. Hirata,, H. Ogawa, and, I. Nagaoka. 2003. Epithelial cell-derived antibacterial peptides human beta-defensins and cathelicidin: multifunctional activities on mast cells. Curr. Drug Targets Inflamm. Allergy 2: 224– 231.
87. Nomura, I.,, E. Goleva,, M. D. Howell,, Q. A. Hamid,, P. Y. Ong,, C. F. Hall,, M. A. Darst,, B. Gao,, M. Boguniewicz,, J. B. Travers, and, D. Y. Leung. 2003. Cytokine milieu of atopic dermatitis, as compared to psoriasis, skin prevents induction of innate immune response genes. J. Immunol. 171: 3262– 3269.
88. Odds, F. C. 1997. Mycology in oral pathology. Acta Stomatol. Belg. 94: 75– 80.
89. Offenbacher, S.,, K. A. Boggess,, A. P. Murtha,, H. L. Jared,, S. Lieff,, R. G. McKaig,, S. M. Mauriello,, K. L. Moss, and, J. D. Beck. 2006. Progressive periodontal disease and risk of very preterm delivery. Obstet. Gynecol. 107: 29– 36.
90. Ogushi, K.,, A. Wada,, T. Niidome,, N. Mori,, K. Oishi,, T. Nagatake,, A. Takahashi,, H. Asakura,, S. Makino,, H. Hojo,, Y. Nakahara,, M. Ohsaki,, T. Hatakeyama,, H. Aoyagi,, H. Kurazono,, J. Moss, and, T. Hirayama. 2001. Salmonella enteritidis FliC (flagella filament protein) induces human beta-defensin-2 mRNA production by Caco-2 cells. J. Biol. Chem. 276: 30521– 30526.
91. Olczak, T.,, W. Simpson,, X. Liu, and, C. A. Genco. 2005. Iron and heme utilization in Porphyromonas gingivalis. FEMS Microbiol. Rev. 29: 119– 144.
92. O’Neil, D. A.,, S. P. Cole,, E. Martin-Porter,, M. P. Housley,, L. Liu,, T. Ganz, and, M. F. Kagnoff. 2000. Regulation of human beta-defensins by gastric epithelial cells in response to infection with Helicobacter pylori or stimulation with interleukin-1. Infect. Immun. 68: 5412– 5415.
93. O’Neil, D. A.,, E. M. Porter,, D. Elewaut,, G. M. Anderson,, L. Eckmann,, T. Ganz, and, M. F. Kagnoff. 1999. Expression and regulation of the human beta-defensins hBD-1 and hBD-2 in intestinal epithelium. J. Immunol. 163: 6718– 6724.
94. Ong, P. Y.,, T. Ohtake,, C. Brandt,, I. Strickland,, M. Boguniewicz,, T. Ganz,, R. L. Gallo, and, D. Y. Leung. 2002. Endogenous antimicrobial peptides and skin infections in atopic dermatitis. N. Engl. J. Med. 347: 1151– 1160.
95. Ouhara, K.,, H. Komatsuzawa,, H. Shiba,, Y. Uchida,, T. Kawai,, K. Sayama,, K. Hashimoto,, M. A. Taubman,, H. Kurihara, and, M. Sugai. 2006. Actinobacillus actinomycetemcomitans outer membrane protein 100 triggers innate immunity and production of beta-defensin and the 18-kilo-dalton cationic antimicrobial protein through the fibronectin-integrin pathway in human gingival epithelial cells. Infect. Immun. 74: 5211– 5220.
96. Ouhara, K.,, H. Komatsuzawa,, S. Yamada,, H. Shiba,, T. Fujiwara,, M. Ohara,, K. Sayama,, K. Hashimoto,, H. Kurihara, and, M. Sugai. 2005. Susceptibilities of periodontopathogenic and cariogenic bacteria to antibacterial peptides, β-defensins and LL37, produced by human epithelial cells. J. Antimicrob. Chemother. 55: 888– 896.
97. Pisano, E.,, T. Cabras,, C. Montaldo,, V. Piras,, R. Inzitari,, C. Olmi,, M. Castagnola, and, I. Messana. 2005. Peptides of human gingival crevicular fluid determined by HPLC-ESI-MS. Eur. J. Oral Sci. 113: 462– 468.
98. Pivarcsi, A.,, I. Nagy,, A. Koreck,, K. Kis,, A. Kenderessy-Szabo,, M. Szell,, A. Dobozy, and, L. Kemeny. 2005. Microbial compounds induce the expression of pro-inflammatory cytokines, chemokines and human beta-defensin-2 in vaginal epithelial cells. Microbes Infect. 7: 1117– 1127.
99. Premratanachai, P.,, S. Joly,, G. K. Johnson,, P. B. McCray, Jr.,, H. P. Jia, and, J. M. Guth-miller. 2004. Expression and regulation of novel human beta-defensins in gingival keratinocytes. Oral Microbiol. Immunol. 19: 111– 117.
100. Proud, D.,, S. P. Sanders, and, S. Wiehler. 2004. Human rhinovirus infection induces air-way epithelial cell production of human beta-defensin 2 both in vitro and in vivo. J. Immunol. 172: 4637– 4645.
101. Richardson, M. D. 2005. Changing patterns and trends in systemic fungal infections. J. Anti-microb. Chemother. 56( Suppl. 1) : i5– i11.
102. Sahly, H.,, S. Schubert,, J. Harder,, P. Rautenberg,, U. Ullmann,, J. Schroder, and, R. Podschun. 2003. Burkholderia is highly resistant to human beta-defensin 3. Antimicrob. Agents Chemother. 47: 1739– 1741.
103. Schenkels, L. C.,, E. C. Veerman, and, A. V. Nieuw Amerongen. 1995. Biochemical composition of human saliva in relation to other mucosal fluids. Crit. Rev. Oral Biol. Med. 6: 161– 175.
104. Schonwetter, B. S.,, E. D. Stolzenberg, and, M. A. Zasloff. 1995. Epithelial antibiotics induced at sites of inflammation. Science 267: 1645– 1648.
105. Schroder, J. M., and, J. Harder. 1999. Human beta-defensin-2. Int. J. Biochem. Cell Biol. 31: 645– 651.
106. Schutte, B. C.,, J. P. Mitros,, J. A. Bartlett,, J. D. Walters,, H. P. Jia,, M. J. Welsh,, T. L. Casavant, and, P. B. McCray, Jr. 2002. Discovery of five conserved beta-defensin gene clusters using a computational search strategy. Proc. Natl. Acad. Sci. USA 99: 2129– 2133.
107. Selsted, M. E.,, S. S. Harwig,, T. Ganz,, J. W. Schilling, and, R. I. Lehrer. 1985. Primary structures of three human neutrophil defensins. J. Clin. Investig. 76: 1436– 1439.
108. Semple, C. A.,, M. Rolfe, and, J. R. Dorin. 2003. Duplication and selection in the evolution of primate beta-defensin genes. Genome Biol. 4: R31.
109. Singh, P. K.,, H. P. Jia,, K. Wiles,, J. Hessel-berth,, L. Liu,, B. A. Conway,, E. P. Greenberg,, E. V. Valore,, M. J. Welsh,, T. Ganz,, B. F. Tack, and, P. B. McCray, Jr. 1998. Production of beta-defensins by human airway epithelia. Proc. Natl. Acad. Sci. USA 95: 14961– 14966.
110. Singh, P. K.,, B. F. Tack,, P. B. McCray, Jr., and, M. J. Welsh. 2000. Synergistic and additive killing by antimicrobial factors found in human airway surface liquid. Am. J. Physiol. Lung Cell. Mol. Physiol. 279: L799– L805.
111. Sorensen, O. E.,, J. B. Cowland,, K. Theil-gaard-Monch,, L. Liu,, T. Ganz, and, N. Borregaard. 2003. Wound healing and expression of antimicrobial peptides/polypeptides in human keratinocytes, a consequence of common growth factors. J. Immunol. 170: 5583– 5589.
112. Sorensen, O. E.,, D. R. Thapa,, A. Rosenthal,, L. Liu,, A. A. Roberts, and, T. Ganz. 2005. Differential regulation of beta-defensin expression in human skin by microbial stimuli. J. Immunol. 174: 4870– 4879.
113. Starner, T. D.,, W. E. Swords,, M. A. Apicella, and, P. B. McCray, Jr. 2002. Susceptibility of nontypeable Haemophilus influenzae to human beta-defensins is influenced by lipooligosaccharide acylation. Infect. Immun. 70: 5287– 5289.
114. Sugawara, Y.,, A. Uehara,, Y. Fujimoto,, S. Kusumoto,, K. Fukase,, K. Shibata,, S. Sugawara,, T. Sasano, and, H. Takada. 2006. Toll-like receptors, NOD1, and NOD2 in oral epithelial cells. J. Dent. Res. 85: 524– 529.
115. Supp, D. M.,, A. C. Karpinski, and, S. T. Boyce. 2004. Expression of human beta-defensins HBD-1, HBD-2, and HBD-3 in cultured keratinocytes and skin substitutes. Burns 30: 643– 648.
116. Taggart, C. C.,, C. M. Greene,, S. G. Smith,, R. L. Levine,, P. B. McCray, Jr.,, S. O’Neill, and, N. G. McElvaney. 2003. Inactivation of human beta-defensins 2 and 3 by elastolytic cathepsins. J. Immunol. 171: 931– 937.
117. Taguchi, Y., and, H. Imai. 2006. Expression of beta-defensin-2 in human gingival epithelial cells in response to challenge with Porphyromonas gingivalis in vitro. J. Periodontal Res. 41: 334– 339.
118. Takahashi, A.,, A. Wada,, K. Ogushi,, K. Maeda,, T. Kawahara,, K. Mawatari,, H. Kurazono,, J. Moss,, T. Hirayama, and, Y. Nakaya. 2001. Production of beta-defensin-2 by human colonic epithelial cells induced by Salmonella enteritidis flagella filament structural protein. FEBS Lett. 508: 484– 488.
119. Tao, R.,, R. J. Jurevic,, K. K. Coulton,, M. T. Tsutsui,, M. C. Roberts,, J. R. Kimball,, N. Wells,, J. Berndt, and, B. A. Dale. 2005. Salivary antimicrobial peptide expression and dental caries experience in children. Antimicrob. Agents Chemother. 49: 3883– 3888.
120. Tarver, A. P.,, D. P. Clark,, G. Diamond,, J. P. Russell,, H. Erdjument-Bromage,, P. Tempst,, K. S. Cohen,, D. E. Jones,, R. W. Sweeney,, M. Wines,, S. Hwang, and, C. L. Bevins. 1998. Enteric beta-defensin: molecular cloning and characterization of a gene with inducible intestinal epithelial cell expression associated with Cryptosporidium parvum infection. Infect. Immun. 66: 1045– 1056.
121. Urban, C. F.,, U. Reichard,, V. Brinkmann, and, A. Zychlinsky. 2006. Neutrophil extracellular traps capture and kill Candida albicans yeast and hyphal forms. Cell. Microbiol. 8: 668– 676.
122. Valore, E. V.,, C. H. Park,, A. J. Quayle,, K. R. Wiles,, P. B. McCray, Jr., and, T. Ganz. 1998. Human beta-defensin-1: an antimicrobial peptide of urogenital tissues. J. Clin. Investig. 101: 1633– 1642.
123. Vankeerberghen, A.,, H. Nuytten,, K. Dierickx,, M. Quirynen,, J. J. Cassiman, and, H. Cuppens. 2005. Differential induction of human beta-defensin expression by periodontal commensals and pathogens in periodontal pocket epithelial cells. J. Periodontol. 76: 1293– 1303.
124. Vankeerberghen, A.,, O. Scudiero,, K. De Boeck,, M. Macek, Jr.,, P. F. Pignatti,, N. Van Hul,, H. Nuytten,, F. Salvatore,, G. Castaldo,, D. Zemkova,, V. Vavrova,, J. J. Cassiman, and, H. Cuppens. 2005. Distribution of human beta-defensin polymorphisms in various control and cystic fibrosis populations. Genomics 85: 574– 581.
125. Voss, E.,, J. Wehkamp,, K. Wehkamp,, E. F. Stange,, J. M. Schroder, and, J. Harder. 2006. NOD2/CARD15 mediates induction of the antimicrobial peptide human beta-defensin-2. J. Biol. Chem. 281: 2005– 2011.
126. Wada, A.,, N. Mori,, K. Oishi,, H. Hojo,, Y. Nakahara,, Y. Hamanaka,, M. Nagashima,, I. Sekine,, K. Ogushi,, T. Niidome,, T. Nagatake,, J. Moss, and, T. Hirayama. 1999. Induction of human beta-defensin-2 mRNA expression by Helicobacter pylori in human gastric cell line MKN45 cells on cag pathogenicity island. Biochem. Biophys. Res. Commun. 263: 770– 774.
127. Wang, X.,, Z. Zhang,, J. P. Louboutin,, C. Moser,, D. J. Weiner, and, J. M. Wilson. 2003. Airway epithelia regulate expression of human beta-defensin 2 through Toll-like receptor 2. FASEB J. 17: 1727– 1729.
128. Wehkamp, J.,, K. Fellermann,, K. R. Herrlinger,, S. Baxmann,, K. Schmidt,, B. Schwind,, M. Duchrow,, C. Wohlschlager,, A. C. Feller, and, E. F. Stange. 2002. Human beta-defensin 2 but not beta-defensin 1 is expressed preferentially in colonic mucosa of inflammatory bowel disease. Eur. J. Gastroenterol. Hepatol. 14: 745– 752.
129. Wehkamp, J.,, K. Fellermann, and, E. F. Stange. 2005. Human defensins in Crohn’s disease. Chem. Immunol. Allergy 86: 42– 54.
130. Wehkamp, J.,, M. Schmid,, K. Fellermann, and, E. F. Stange. 2005. Defensin deficiency, intestinal microbes, and the clinical phenotypes of Crohn’s disease. J. Leukoc. Biol. 77: 460– 465.
131. Wehkamp, J.,, K. Schmidt,, K. R. Herrlinger,, S. Baxmann,, S. Behling,, C. Wohlschlager,, A. C. Feller,, E. F. Stange, and, K. Fellermann. 2003. Defensin pattern in chronic gastritis: HBD-2 is differentially expressed with respect to Helicobacter pylori status. J. Clin. Pathol. 56: 352– 357.
132. Wehkamp, K.,, L. Schwichtenberg,, J. M. Schroder, and, J. Harder. 2006. Pseudomonas aeruginosa- and IL-1beta-mediated induction of human beta-defensin-2 in keratinocytes is controlled by NF-kappaB and AP-1. J. Investig. Dermatol. 126: 121– 127.
133. Wohlfahrt, J. C.,, T. Wu,, J. S. Hodges,, J. E. Hinrichs, and, B. S. Michalowicz. 2006. No association between selected candidate gene polymorphisms and severe chronic periodontitis. J. Periodontol. 77: 426– 436.
134. Xiong, X.,, P. Buekens,, W. D. Fraser,, J. Beck, and, S. Offenbacher. 2006. Periodontal disease and adverse pregnancy outcomes: a systematic review. BJOG 113: 135– 143.
135. Yang, D.,, A. Biragyn,, D. M. Hoover,, J. Lubkowski, and, J. J. Oppenheim. 2004. Multiple roles of antimicrobial defensins, cathelicidins, and eosinophil-derived neurotoxin in host defense. Annu. Rev. Immunol. 22: 181– 215.
136. Yang, D.,, O. Chertov, and, J. J. Oppenheim. 2001. The role of mammalian antimicrobial peptides and proteins in awakening of innate host defenses and adaptive immunity. Cell. Mol. Life Sci. 58: 978– 989.
137. Zaalouk, T. K.,, M. Bajaj-Elliott,, J. T. George, and, V. McDonald. 2004. Differential regulation of beta-defensin gene expression during Cryptosporidium parvum infection. Infect. Immun. 72: 2772– 2779.
138. Zhao, C.,, I. Wang, and, R. I. Lehrer. 1996. Widespread expression of beta-defensin hBD-1 in human secretory glands and epithelial cells. FEBS Lett. 396: 319– 322.

Tables

Effect of Defensins on Susceptibility to Infection at the Mucosal Surface
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Citation: Guthmiller J, Joly S. 2007. Effect of Defensins on Susceptibility to Infection at the Mucosal Surface, p 265-280. In Brogden K, Minion F, Cornick N, Stanton T, Zhang Q, Nolan L, Wannemuehler M (ed), Virulence Mechanisms of Bacterial Pathogens, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815851.ch18
/content/10.1128/9781555815851.ch18.ch18tab01
TABLE 1
Differential induction of HBD-1, HBD-2, and HBD-3 by IL-1β, IFN-γ, and TNF-α as assessed by real-time PCR
Generic image for table
TABLE 1

Differential induction of HBD-1, HBD-2, and HBD-3 by IL-1β, IFN-γ, and TNF-α as assessed by real-time PCR

Citation: Guthmiller J, Joly S. 2007. Effect of Defensins on Susceptibility to Infection at the Mucosal Surface, p 265-280. In Brogden K, Minion F, Cornick N, Stanton T, Zhang Q, Nolan L, Wannemuehler M (ed), Virulence Mechanisms of Bacterial Pathogens, Fourth Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815851.ch18

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