Chapter 31 : Large-Scale Production of Microorganisms

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This chapter highlights the selection and design of equipment and facilities to achieve a safe work environment. There are few absolutes that can be applied across the board, since the decisions to be made are dependent on the risk assessment of the organism and the processes used. Fortunately, similar equipment and facility design criteria are used, so there are common biosafety principles that can be utilized which are discussed herein. Primary containment is provided by the equipment utilized and the use of appropriate biosafety practices, administrative practices, and personal protective equipment. In general, all of the standard and special practices and safety equipment identified in (BMBL), along with the recommendations in the NIH recombinant-DNA guidelines, are applicable to large-scale processes. Bioreactors used for growth of microorganisms and those used for cell culture can share many attributes. A biological safety cabinet (BSC) can be utilized for smaller equipment that does not generate much turbulence. In some instances, BSC manufacturers, or other specialty equipment fabricators, can make specialized containment devices for specific equipment. The facility design and construction provide the secondary containment that protects people outside of the immediate work area, both in other parts of the facility and in the community at large.

Citation: Cipriano M. 2006. Large-Scale Production of Microorganisms, p 561-577. In Fleming D, Hunt D (ed), Biological Safety. ASM Press, Washington, DC. doi: 10.1128/9781555815899.ch31
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17. World Health Organization. 2004. Laboratory Biosafety Manual. 3rd ed. World Health Organization, Geneva, Switzerland.

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