Chemokine and Chemokine Receptor Analysis

Benjamin D. Medoff; Andrew D. Luster

doi:10.1128/9781555815905.ch42

Chapter 42 : Chemokine and Chemokine Receptor Analysis

Authors: Benjamin D. Medoff, Andrew D. Luster

Content Type: Reference

Publication Year: 2006

Category: Immunology

Book DOI: 10.1128/9781555815905

Chapter DOI: 10.1128/9781555815905.ch42

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Abstract:

This chapter describes the methodological approaches to studying the role of chemokines and chemokine receptors in the physiology of immune and inflammatory responses. Chemokines share the common function of attracting leukocytes to sites of an inflammatory or immune response. The G protein-coupled cell surface receptors (GPCRs) as signal transducers of chemotaxis appear to be highly conserved in evolution, being present on amoebae and slime molds, and are involved in the signaling of chemotaxis in these simple eukaryotes. Activation of chemokine receptors is usually accompanied by a transient rise in the level of intracellular calcium. The attraction of leukocytes to sites of inflammation and infection is an essential component of the host response to disease. Chemokines and chemokine receptors have been shown to be an integral part of this process and have been implicated in the pathophysiology of many infectious diseases and inflammatory disorders. The recruited monocytes and T lymphocytes are thought to play a key role in the pathogenesis of atherosclerotic plaque, and thus the molecular signals that attract these cells into the lesions are likely important for lesion formation. The majority of cells recruited into the synovium are neutrophils and mononuclear leukocytes that presumably help propagate the inflammation and joint destruction. Chemokine expression in the lung or blood could be used as a reliable noninvasive marker of inflammation in the airways and thus would be of great value in the management of asthma.

Citation: Medoff B, Luster A. 2006. Chemokine and Chemokine Receptor Analysis, p 371-384. In Detrick B, Hamilton R, Folds J (ed), Manual of Molecular and Clinical Laboratory Immunology, 7th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815905.ch42

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Immune Receptors: 0.48924622
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Chemokine and Chemokine Receptor Analysis

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/content/10.1128/9781555815905.ch42.ch42fig01

FIGURE 1

Chemokine receptor signal transduction. Chemokine receptors are a subfamily of seven-transmembrane-spanning GPCRs. They are coupled to heterotrimeric G proteins of the Gi subclass, which are distinguished by their pertussis toxin sensitivity. Chemokine receptor activation leads to the stimulation of multiple signal transduction pathways, including the activation of PI 3-kinase (PI-3K) and phospholipase C, leading to the generation of inositol triphosphates, intracellular calcium release, and PKC activation. Chemokine signaling also induces the upregulation of integrin affinity and the activation of Rho, leading to cytoskeletal reorganization. Agonist-stimulated receptors also activate G protein receptor kinases, which leads to receptor phosphorylation, arrestin binding, G protein uncoupling (desensitization), and clathrin-mediated receptor endocy-tosis (internalization). LTB4, leukotriene B4; C5a, complement fragment 5a; PAF, platelet activating factor; cAMP cyclic AMP; PLC β2, phospholipase C β2; DAG, diacylglycerol.

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FIGURE 1

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FIGURE 2

Transmigration assay system. Positive, negative, and absent gradients of a chemokine are established in order to assess chemotaxis (movement towards a chemokine) and chemokinesis (random movement of cells in response to a chemokine in the absence of a gradient). Cells are plated into the upper chamber of the transwell system or Boyden chamber, and the proportion of migrating cells is determined by accurate counting of cells which migrate to the lower chamber. The upper and lower chambers are separated by a polycarbonate membrane of standard pore size (between 3 and 8 μm), dependent on the migrating cell type.

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FIGURE 2

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FIGURE 3

Digitized time lapse photography of cells moving in the presence of a gradient of a chemokine. Positive and negative gradients of a chemokine can be established in methylcellulose as previously described ( 52 ). Cells are plated into methylcellulose, and a gradient is established by inoculating the methylcellulose at a fixed point with the chemokine. Cells are then visualized migrating in response to the gradient by using time lapse video microscopy.

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FIGURE 3

References

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1. Abdi, R.,, T. K. Means,, T. Ito,, R. N. Smith,, N. Najafian,, M. Jurewicz,, V. Tchipachvili,, I. Charo,, H. Auchincloss, Jr.,, M. H. Sayegh, and , A. D. Luster. 2004. Differential role of CCR2 in islet and heart allograft rejection: tissue specificity of chemokine/chemokine receptor function in vivo. J. Immunol. 172: 767– 775.

2. Abdi, R.,, R. N. Smith,, L. Makhlouf,, N. Najafian,, A. D. Luster,, H. Auchincloss, Jr., and , M. H. Sayegh. 2002. The role of CC chemokine receptor 5 (CCR5) in islet allograft rejection. Diabetes 51: 2489– 2495.

3. Abi-Younes, S.,, A. Sauty,, F. Mach,, G. K. Sukhova,, P. Libby, and , A. D. Luster. 2000. The stromal cellderived factor-1 chemokine is a potent platelet agonist highly expressed in atherosclerotic plaques. Circ. Res. 86: 131– 138.

4. Ajuebor, M. N.,, M. G. Swain, and , M. Perretti. 2002. Chemokines as novel therapeutic targets in inflammatory diseases. Biochem. Pharmacol. 63: 1191– 1196.

5. Ali, S.,, A. C. Palmer,, B. Banerjee,, S. J. Fritchley, and , J. A. Kirby. 2000. Examination of the function of RANTES, MIP-1alpha, and MIP-1beta following interaction with heparinlike glycosaminoglycans. J. Biol. Chem. 275: 11721– 11727.

6. Baggiolini, M. 1998. Chemokines and leukocyte traffic. Nature 392: 565– 568.

7. Becknew, S. 1997. G-protein activation by chemokines. Methods Enzymol. 298: 309– 326.

8. Belperio, J. A.,, M. D. Burdick,, M. P. Keane,, Y. Y. Xue,, J. P. Lynch III,, B. L. Daugherty,, S. L. Kunkel, and , R. M. Strieter. 2000. The role of the CC chemokine, RANTES, in acute lung allograft rejection. J. Immunol. 165: 461– 472.

9. Belperio, J. A.,, M. P. Keane,, M. D. Burdick,, J. P. Lynch III,, Y. Y. Xue,, A. Berlin,, D. J. Ross,, S. L. Kunkel,, I. F. Charo, and , R. M. Strieter. 2001. Critical role for the chemokine MCP-1/CCR2 in the pathogenesis of bronchiolitis obliterans syndrome. J. Clin. Investig. 108: 547– 556.

10. Belperio, J. A.,, M. P. Keane,, M. D. Burdick,, J. P. Lynch III,, Y. Y. Xue,, K. Li,, D. J. Ross, and , R. M. Strieter. 2002. Critical role for CXCR3 chemokine biology in the pathogenesis of bronchiolitis obliterans syndrome. J. Immunol. 169: 1037– 1049.

11. Belperio, J. A.,, M. P. Keane,, M. D. Burdick,, J. P. Lynch III,, D. A. Zisman,, Y Y Xue,, K. Li,, A. Ardehali,, D. J. Ross, and , R. M. Strieter. 2003. Role of CXCL9/CXCR3 chemokine biology during pathogenesis of acute lung allograft rejection. J. Immunol. 171: 4844– 4852.

12. Berkhout, T. A.,, H. M. Sarau,, K. Moores,, J. R. White,, N. Elshourbagy,, E. Appelbaum,, R. J. Reape,, M. Brawner,, J. Makwana,, J. J. Foley,, D. B. Schmidt,, C. Imburgia,, D. McNulty,, J. Matthews,, K. O’Donnell,, D. O’Shannessy,, M. Scott,, P. H. Groot, and , C. Macphee. 1997. Cloning, in vitro expression, and functional characterization of a novel human CC chemokine of the monocyte chemotactic protein (MCP) family (MCP-4) that binds and signals through the CC chemokine receptor 2B. J. Biol. Chem. 272: 16404– 16413.

13. Bird, J. E.,, M. R. Giancarli,, T. Kurihara,, M. C. Kowala,, M. T. Valentine,, P. H. Gitlitz,, D. G. Pandya,, M. H. French, and , S. K. Durham. 2000. Increased severity of glomerulonephritis in C-C chemokine receptor 2 knockout mice. Kidney Int. 57: 129– 136.

14. Black, D. 2000. Endothelial cell chemotaxis assays. Methods Mol. Biol. 138: 121– 127.

15. Bleul, C. C.,, R. C. Fuhlbrigge,, J. M. Casasnovas,, A. Aiuti, and , T. A. Springer. 1996. A highly efficacious lymphocyte chemoattractant, stromal cell-derived factor 1 (SDF-1). J. Exp. Med. 184: 1101– 1119.

16. Bonecchi, R.,, G. Bianchi,, P. P. Bordignon,, D. D’Ambrosio,, R. Lang,, A. Borsatti,, S. Sozzani,, P. Allavena,, P. A. Gray,, A. Mantovani, and , F. Sinigaglia. 1998. Differential expression of chemokine receptors and chemotactic responsiveness of type 1 T helper cells (Th1s) and Th2s. J. Exp. Med. 187: 129– 134.

17. Boring, L.,, J. Gosling,, S. W. Chensue,, S. L. Kunkel,, R. V. Farese, Jr.,, H. E. Broxmeyer, and , I. F. Charo. 1997. Impaired monocyte migration and reduced type 1 (Th1) cytokine responses in C-C chemokine receptor 2 knockout mice. J. Clin. Investig. 100: 2552– 2561.

18. Boring, L.,, J. Gosling,, M. Cleary, and , I. F. Charo. 1998. Decreased lesion formation in CCR2—/—mice reveals a role for chemokines in the initiation of atherosclerosis. Nature 394: 894– 897.

19. Boyden, S. 1962. The chemotactic effect of mixtures of antibody and antigen on polymorphonuclear leucocytes. J. Exp. Med. 115: 453– 466.

20. Buser, R., and , A. E. Proudfoot. 2000. Calcium mobilization. Methods Mol. Biol. 138: 143– 148.

21. Busse, W. W., and , R. F. Lemanske, Jr. 2001. Asthma. N. Engl. J. Med. 344: 350– 362.

22. Cacalano, G.,, J. Lee,, K. Kikly,, A. M. Ryan,, S. Pitts-Meek,, B. Hultgren,, W. I. Wood, and , M. W. Moore. 1994. Neutrophil and B cell expansion in mice that lack the murine IL-8 receptor homolog. Science 265: 682– 684.

23. Carbone, F. R.,, S. J. Sterry,, J. Butler,, S. Rodda, and , M. W. Moore. 1992. T cell receptor alpha-chain pairing determines the specificity of residue 262 within the Kb-restricted, ovalbumin257-264 determinant. Int. Immunol. 4: 861– 867.

24. Cobbold, P. H., and , T. J. Rink. 1987. Fluorescence and bioluminescence measurement of cytoplasmic free calcium. Biochem. J. 248: 313– 328.

25. Das, A. M.,, M. N. Ajuebor,, R. J. Flower,, M. Perretti, and , S. R. McColl. 1999. Contrasting roles for RANTES and macrophage inflammatory protein-1 alpha (MIP-1 alpha) in a murine model of allergic peritonitis. Clin. Exp. Immunol. 117: 223– 229.

26. de Lemos, J. A.,, D. A. Morrow,, M. S. Sabatine,, S. A. Murphy,, C. M. Gibson,, E. M. Antman,, C. H. McCabe,, C. P. Cannon, and , E. Braunwald. 2003. Association between plasma levels of monocyte chemoattractant protein-1 and long-term clinical outcomes in patients with acute coronary syndromes. Circulation 107: 690– 695.

27. del Pozo, M. A.,, M. Vicente-Manzanares,, R. Tejedor,, J. M. Serrador, and , F. Sanchez-Madrid. 1999. Rho GTPases control migration and polarization of adhesion molecules and cytoskeletal ERM components in T lymphocytes. Eur. J. Immunol. 29: 3609– 3620.

28. De Perrot, M.,, Y. Sekine,, S. Fischer,, T. K. Waddell,, K. McRae,, M. Liu,, D. A. Wigle, and , S. Keshavjee. 2002. Interleukin-8 release during early reperfusion predicts graft function in human lung transplantation. Am. J. Respir. Crit. Care Med. 165: 211– 215.

29. De Perrot, M.,, K. Young,, Y. Imai,, M. Liu,, T. K. Waddell,, S. Fischer,, L. Zhang, and , S. Keshavjee. 2003. Recipient T cells mediate reperfusion injury after lung transplantation in the rat. J. Immunol. 171: 4995– 5002.

30. De Sanctis, G. T.,, J. A. MacLean,, S. Qin,, W. W. Wolyniec,, H. Grasemann,, C. N. Yandava,, A. Jiao,, T. Noonan,, J. Stein-Streilein,, F. H. Green, and , J. M. Drazen. 1999. Interleukin-8 receptor modulates IgE production and B-cell expansion and trafficking in allergen-induced pulmonary inflammation. J. Clin. Investig. 103: 507– 515.

31. DeVries, M. E.,, K. A. Hosiawa,, C. M. Cameron,, S. E. Bosinger,, D. Persad,, A. A. Kelvin,, J. C. Coombs,, H. Wang,, R. Zhong,, M. J. Cameron, and , D. J. Kelvin. 2003. The role of chemokines and chemokine receptors in alloantigen-independent and alloantigen-dependent transplantation injury. Semin. Immunol. 15: 33– 48.

32. DeVries, M. E.,, L. Ran, and , D. J. Kelvin. 1999. On the edge: the physiological and pathophysiological role of chemokines during inflammatory and immunological responses. Semin. Immunol. 11: 95– 104.

33. Dryer, L. 2000. Evolution of odorant receptors. Bioessays 22: 803– 810.

34. Dustin, M. L., and , A. C. Chan. 2000. Signaling takes shape in the immune system. Cell 103: 283– 294.

35. Eitner, F.,, Y. Cui,, K. L. Hudkins, and , C. E. Alpers. 1998. Chemokine receptor (CXCR4) mRNA-expressing leukocytes are increased in human renal allograft rejection. Transplantation 66: 1551– 1557.

36. Falk, W.,, R. H. Goodwin, Jr., and , E. J. Leonard. 1980. A 48-well micro chemotaxis assembly for rapid and accurate measurement of leukocyte migration. J. Immunol. Methods 33: 239– 247.

37. Gao, W.,, P. S. Topham,, J. A. King,, S. T. Smiley,, V. Csizmadia,, B. Lu,, C. J. Gerard, and , W. W. Hancock. 2000. Targeting of the chemokine receptor CCR1 suppresses development of acute and chronic cardiac allograft rejection. J. Clin. Investig. 105: 35– 44.

38. Garlichs, C. D.,, S. John,, A. Schmeisser,, S. Eskafi,, C. Stumpf,, M. Karl,, M. Goppelt-Struebe,, R. Schmieder, and , W. G. Daniel. 2001. Upregulation of CD40 and CD40 ligand (CD154) in patients with moderate hypercholes-terolemia. Circulation 104: 2395– 2400.

39. Garred, P.,, H. O. Madsen,, J. Petersen,, H. Marquart,, T. M. Hansen,, S. Freiesleben Sorensen,, B. Volck,, A. Svejgaard, and , V. Andersen. 1998. CC chemokine receptor 5 polymorphism in rheumatoid arthritis. J. Rheumatol. 25: 1462– 1465.

40. Gerszten, R. E.,, F. Mach,, A. Sauty,, A. Rosenzweig, and , A. D. Luster. 2000. Chemokines, leukocytes, and atherosclerosis. J. Lab. Clin. Med. 136: 87– 92.

41. Goddard, S.,, A. Williams,, C. Morland,, S. Qin,, R. Gladue,, S. G. Hubscher, and , D. H. Adams. 2001. Differential expression of chemokines and chemokine receptors shapes the inflammatory response in rejecting human liver transplants. Transplantation 72: 1957– 1967.

42. Gong, J. H.,, L. G. Ratkay,, J. D. Waterfield, and , I. Clark-Lewis. 1997. An antagonist of monocyte chemoattractant protein 1 (MCP-1) inhibits arthritis in the MRL-lpr mouse model. J. Exp. Med. 186: 131– 137.

43. Grynkiewicz, G.,, M. Poenie, and , R. Y. Tsien. 1985. A new generation of Ca 2+ indicators with greatly improved fluorescence properties. J. Biol. Chem. 260: 3440– 3450.

44. Gu, L.,, Y. Okada,, S. K. Clinton,, C. Gerard,, G. K. Sukhova,, P. Libby, and , B. J. Rollins. 1998. Absence of monocyte chemoattractant protein-1 reduces atherosclerosis in low density lipoprotein receptor-deficient mice. Mol. Cell 2: 275– 281.

45. Hachicha, M.,, P. Rathanaswami,, T. J. Schall, and , S. R. McColl. 1993. Production of monocyte chemotactic protein-1 in human type B synoviocytes. Synergistic effect of tumor necrosis factor alpha and interferon-gamma. Arthritis Rheum. 36: 26– 34.

46. Halloran, M. M.,, J. M. Woods,, R. M. Strieter,, Z. Szekanecz,, M. V. Volin,, S. Hosaka,, G. K. Haines III,, S. L. Kunkel,, M. D. Burdick,, A. Walz, and , A. E. Koch. 1999. The role of an epithelial neutrophil-activating pep-tide-78-like protein in rat adjuvant-induced arthritis. J. Immunol. 162: 7492– 7500.

47. Hancock, W. W.,, B. Lu,, W. Gao,, V. Csizmadia,, K. Faia,, J. A. King,, S. T. Smiley,, M. Ling,, N. P. Gerard, and , C. Gerard. 2000. Requirement of the chemokine receptor CXCR3 for acute allograft rejection. J. Exp. Med. 192: 1515– 1520.

48. Hanto, D. W.,, U. T. Hopt,, R. Hoffman, and , R. L. Simmons. 1982. Lymphocyte recruitment, regional blood flow, and vascular permeability at sites of allogeneic cellular interactions. J. Immunol. 129: 2437– 2443.

49. Hanto, D. W.,, U. T. Hopt,, R. Hoffman, and , R. L. Simmons. 1982. Recruitment of unsensitized circulating lymphocytes to sites of allogeneic cellular interactions. Transplantation 33: 541– 546.

50. Harigai, M.,, M. Hara,, T. Yoshimura,, E. J. Leonard,, K. Inoue, and , S. Kashiwazaki. 1993. Monocyte chemoattractant protein-1 (MCP-1) in inflammatory joint diseases and its involvement in the cytokine network of rheumatoid synovium. Clin. Immunol. Immunopathol. 69: 83– 91.

51. Haskell, C. A.,, W. W. Hancock,, D. J. Salant,, W. Gao,, V. Csizmadia,, W. Peters,, K. Faia,, O. Fituri,, J. B. Rottman, and , I. F. Charo. 2001. Targeted deletion of CX(3)CR1 reveals a role for fractalkine in cardiac allograft rejection. J. Clin. Investig. 108: 679– 688.

52. Hirsch, E.,, V. L. Katanaev,, C. Garlanda,, O. Azzolino,, L. Pirola,, L. Silengo,, S. Sozzani,, A. Mantovani,, F. Altruda, and , M. P. Wymann. 2000. Central role for G protein-coupled phosphoinositide 3-kinase gamma in inflammation. Science 287: 1049– 1053.

53. Horuk, R. (ed.). 1997. Methods in Enzymology, vol. 288. Chemokine Receptors. Academic Press, San Diego, Calif.

54. Hosaka, S.,, T. Akahoshi,, C. Wada, and , H. Kondo. 1994. Expression of the chemokine superfamily in rheumatoid arthritis. Clin. Exp. Immunol. 97: 451– 457.

55. Jaffer, F. A., and , R. Weissleder. 2004. Seeing within: molecular imaging of the cardiovascular system. Circ. Res. 94: 433– 445.

56. Jourdan, P.,, J. P. Vendrell,, M. F. Huguet,, M. Segondy,, J. Bousquet,, J. Pene, and , H. Yssel. 2000. Cytokines and cell surface molecules independently induce CXCR4 expression on CD4+ CCR7+ human memory T cells. J. Immunol. 165: 716– 724.

57. Kao, J.,, J. Kobashigawa,, M. C. Fishbein,, W. R. MacLellan,, M. D. Burdick,, J. A. Belperio, and , R. M. Strieter. 2003. Elevated serum levels of the CXCR3 chemokine ITAC are associated with the development of transplant coronary artery disease. Circulation 107: 1958– 1961.

58. Khan, I. A.,, J. A. MacLean,, F. S. Lee,, L. Casciotti,, E. DeHaan,, J. D. Schwartzman, and , A. D. Luster. 2000. IP-10 is critical for effector T cell trafficking and host survival in Toxoplasma gondii infection. Immunity 12: 483– 494.

59. Kim, J. Y.,, J. A. Borleis, and , P. N. Devreotes. 1998. Switching of chemoattractant receptors programs development and morphogenesis in Dictyostelium: receptor subtypes activate common responses at different agonist concentrations. Dev. Biol. 197: 117– 128.

60. Koch, A. E.,, S. L. Kunkel,, L. A. Harlow,, D. D. Mazarakis,, G. K. Haines,, M. D. Burdick,, R. M. Pope, and , R. M. Strieter. 1994. Macrophage inflammatory protein-1 alpha: a novel chemotactic cytokine for macrophages in rheumatoid arthritis. J. Clin. Investig. 93: 921– 928.

61. Koch, A. E.,, S. L. Kunkel,, L. A. Harlow,, D. D. Mazarakis,, G. K. Haines,, M. D. Burdick,, R. M. Pope,, A. Walz, and , R. M. Strieter. 1994. Epithelial neutrophil activating peptide-78: a novel chemotactic cytokine for neutrophils in arthritis. J. Clin. Investig. 94: 1012– 1018.

62. Koch, A. E.,, S. L. Kunkel,, M. R. Shah,, S. Hosaka,, M. M. Halloran,, G. K. Haines,, M. D. Burdick,, R. M. Pope, and , R. M. Strieter. 1995. Growth-related gene product alpha: a chemotactic cytokine for neutrophils in rheumatoid arthritis. J. Immunol. 155: 3660– 3666.

63. Koch, A. E.,, M. V. Volin,, J. M. Woods,, S. L. Kunkel,, M. A. Connors,, L. A. Harlow,, D. C. Woodruff,, M. D. Burdick, and , R. M. Strieter. 2001. Regulation of angiogenesis by the C-X-C chemokines interleukin-8 and epithelial neutrophil activating peptide 78 in the rheumatoid joint. Arthritis Rheum. 44: 31– 40.

64. Krukemeyer, M. G.,, J. Moeller,, L. Morawietz,, B. Rudolph,, U. Neumann,, T. Theruvath,, P. Neuhaus, and , V. Krenn. 2004. Description of B lymphocytes and plasma cells, complement, and chemokines/receptors in acute liver allograft rejection. Transplantation 78: 65– 70.

65. Kullich, W. C., and , G. Klein. 1998. High levels of macrophage inflammatory protein-1alpha correlate with prolactin in female patients with active rheumatoid arthritis. Clin. Rheumatol. 17: 263– 264.

66. Kunkel, S. L. 1999. Through the looking glass: the diverse in vivo activities of chemokines. J. Clin. Investig. 104: 1333– 1334.

67. Kurashima, K.,, N. Mukaida,, M. Fujimura,, J. M. Schroder,, T. Matsuda, and , K. Matsushima. 1996. Increase of chemokine levels in sputum precedes exacerbation of acute asthma attacks. J. Leukoc. Biol. 59: 313– 316.

68. Kuziel, W. A.,, S. J. Morgan,, T. C. Dawson,, S. Griffin,, O. Smithies,, K. Ley, and , N. Maeda. 1997. Severe reduction in leukocyte adhesion and monocyte extravasation in mice deficient in CC chemokine receptor 2. Proc. Natl. Acad. Sci. USA 94: 12053– 12058.

69. Lamkhioued, B.,, E. A. Garcia-Zepeda,, S. Abi-Younes,, H. Nakamura,, S. Jedrzkiewicz,, L. Wagner,, P. M. Renzi,, Z. Allakhverdi,, C. Lilly,, Q. Hamid, and , A. D. Luster. 2000. Monocyte chemoattractant protein (MCP)-4 expression in the airways of patients with asthma. Induction in epithelial cells and mononuclear cells by proinflammatory cytokines. Am. J. Respir. Crit. Care Med. 162: 723– 732.

70. Laudanna, C.,, J. J. Campbell, and , E. C. Butcher. 1996. Role of Rho in chemoattractant-activated leukocyte adhesion through integrins. Science 271: 981– 983.

71. Leung, T. F.,, G. W. Wong,, F. W. Ko,, C. W. Lam, and , T. F. Fok. 2004. Increased macrophage-derived chemokine in exhaled breath condensate and plasma from children with asthma. Clin. Exp. Allergy 34: 786– 791.

72. Libby, P., and , P. M. Ridker. 2004. Inflammation and atherosclerosis: role of C-reactive protein in risk assessment. Am. J. Med. 116 (Suppl. 6A): 9S– 16S.

73. Loetscher, M.,, P. Loetscher,, N. Brass,, E. Meese, and , B. Moser. 1998. Lymphocyte-specific chemokine receptor CXCR3: regulation, chemokine binding and gene localization. Eur. J. Immunol. 28: 3696– 3705.

74. Lu, B.,, B. J. Rutledge,, L. Gu,, J. Fiorillo,, N. W. Lukacs,, S. L. Kunkel,, R. North,, C. Gerard, and , B. J. Rollins. 1998. Abnormalities in monocyte recruitment and cytokine expression in monocyte chemoattractant protein 1-deficient mice. J. Exp. Med. 187: 601– 608.

75. Lukacs, N. W.,, A. Berlin,, D. Schols,, R. T. Skerlj, and , G. J. Bridger. 2002. AMD3100, a CxCR4 antagonist, attenuates allergic lung inflammation and airway hyperreactivity. Am. J. Pathol. 160: 1353– 1360.

76. Lukacs, N. W.,, D. M. Prosser,, M. Wiekowski,, S. A. Lira, and , D. N. Cook. 2001. Requirement for the chemokine receptor CCR6 in allergic pulmonary inflammation. J. Exp. Med. 194: 551– 555.

77. Lukacs, N. W.,, R. M. Strieter,, S. W. Chensue, and , S. L. Kunkel. 1996. Activation and regulation of chemokines in allergic airway inflammation. J. Leukoc. Biol. 59: 13– 17.

78. Luster, A. D.,, S. Abi-Younes, and , A. Tager. 2000. Chemokines and disease, p. 339– 382. In M. E. Rothenberg (ed.), Chemokines in Allergic Disease. Marcel Dekker AG, New York, N.Y.

79. Luster, A. D.,, R. D. Cardiff,, J. A. MacLean,, K. Crowe, and , R. D. Granstein. 1998. Delayed wound healing and disorganized neovascularization in transgenic mice expressing the IP-10 chemokine. Proc. Assoc. Am. Physicians 110: 183– 196.

80. Luster, A. D., and , A. M. Tager. 2004. T-cell trafficking in asthma: lipid mediators grease the way. Nat. Rev. Immunol. 4: 711– 724.

81. Ma, Q.,, D. Jones,, P. R. Borghesani,, R. A. Segal,, T. Nagasawa,, T. Kishimoto,, R. T. Bronson, and , T. A. Springer. 1998. Impaired B-lymphopoiesis, myelopoiesis, and derailed cerebellar neuron migration in CXCR4- and SDF-1-deficient mice. Proc. Natl. Acad. Sci. USA 95: 9448– 9453.

82. Mach, F.,, A. Sauty,, A. S. Iarossi,, G. K. Sukhova,, K. Neote,, P. Libby, and , A. D. Luster. 1999. Differential expression of three T lymphocyte-activating CXC chemokines by human atheroma-associated cells. J. Clin. Investig. 104: 1041– 1050.

83. Matsui, T.,, T. Akahoshi,, R. Namai,, A. Hashimoto,, Y. Kurihara,, M. Rana,, A. Nishimura,, H. Endo,, H. Kitasato,, S. Kawai,, K. Takagishi, and , H. Kondo. 2001. Selective recruitment of CCR6-expressing cells by increased production of MIP-3 alpha in rheumatoid arthritis. Clin. Exp. Immunol. 125: 155– 161.

84. Matthews, A. N.,, D. S. Friend,, N. Zimmermann,, M. N. Sarafi,, A. D. Luster,, E. Pearlman,, S. E. Wert, and , M. E. Rothenberg. 1998. Eotaxin is required for the baseline level of tissue eosinophils. Proc. Natl. Acad. Sci. USA 95: 6273– 6278.

85. Medoff, B. D.,, A. Sauty,, A. M. Tager,, J. A. Maclean,, R. N. Smith,, A. Mathew,, J. H. Dufour, and , A. D. Luster. 2002. IFN-gamma-inducible protein 10 (CXCL10) contributes to airway hyperreactivity and airway inflammation in a mouse model of asthma. J. Immunol. 168: 5278– 5286.

86. Miotto, D.,, P. Christodoulopoulos,, R. Olivenstein,, R. Taha,, L. Cameron,, A. Tsicopoulos,, A. B. Tonnel,, O. Fahy,, J. J. Lafitte,, A. D. Luster,, B. Wallaert,, C. E. Mapp, and , Q. Hamid. 2001. Expression of IFN-gamma-inducible protein; monocyte chemotactic proteins 1, 3, and 4; and eotaxin in TH1- and TH2-mediated lung diseases. J. Allergy. Clin. Immunol. 107: 664– 670.

87. Mombaerts, P.,, F. Wang,, C. Dulac,, R. Vassar,, S. K. Chao,, A. Nemes,, M. Mendelsohn,, J. Edmondson, and , R. Axel. 1996. The molecular biology of olfactory perception. Cold Spring Harbor Symp. Quant. Biol. 61: 135– 145.

88. Moshfegh, A.,, G. Hallden, and , J. Lundahl. 1999. Methods for simultaneous quantitative analysis of eosinophil and neutrophil adhesion and transmigration. Scand.J. Immunol. 50: 262– 269.

89. Nagasawa, T.,, S. Hirota,, K. Tachibana,, N. Takakura,, S. Nishikawa,, Y. Kitamura,, N. Yoshida,, H. Kikutani, and , T. Kishimoto. 1996. Defects of B-cell lymphopoiesis and bone-marrow myelopoiesis in mice lacking the CXC chemokine PBSF/SDF-1. Nature 382: 635– 658.

90. Nanki, T.,, K. Hayashida,, H. S. El-Gabalawy,, S. Suson,, K. Shi,, H. J. Girschick,, S. Yavuz, and , P. E. Lipsky. 2000. Stromal cell-derived factor-1-CXC chemokine receptor 4 interactions play a central role in CD4+ T cell accumulation in rheumatoid arthritis synovium. J. Immunol. 165: 6590– 6598.

91. Nelken, N. A.,, S. R. Coughlin,, D. Gordon, and , J. N. Wilcox. 1991. Monocyte chemoattractant protein-1 in human atheromatous plaques. J. Clin. Investig. 88: 1121– 1127.

92. Parent, C. A.,, B. J. Blacklock,, W. M. Froehlich,, D. B. Murphy, and , P. N. Devreotes. 1998. G protein signaling events are activated at the leading edge of chemotactic cells. Cell 95: 81– 91.

93. Patel, D. D.,, J. P. Zachariah, and , L. P. Whichard. 2001. CXCR3 and CCR5 ligands in rheumatoid arthritis synovium. Clin. Immunol. 98: 39– 45.

94. Piemonti, L.,, G. Calori,, A. Mercalli,, G. Lattuada,, P. Monti,, M. P. Garancini,, F. Costantino,, G. Ruotolo,, L. Luzi, and , G. Perseghin. 2003. Fasting plasma leptin, tumor necrosis factor-α receptor 2, and monocyte chemoattracting protein 1 concentration in a population of glucose-tolerant and glucose-intolerant women: impact on cardiovascular mortality. Diabetes Care 26: 2883– 2889.

95. Ponath, P.,, N. Kassam, and , S. Qin. 2000. Monoclonal antibodies to chemokine receptors. Methods Mol. Biol: 138: 231– 242.

96. Power, C. A. 2003. Knock out models to dissect chemokine receptor function in vivo. J. Immunol. Methods 273: 73– 82.

97. Poznansky, M. C.,, I. T. Olszak,, R. Foxall,, R. H. Evans,, A. D. Luster, and , D. T. Scadden. 2000. Active movement of T cells away from a chemokine. Nat. Med. 6: 543– 548.

98. Rathanaswami, P.,, M. Hachicha,, M. Sadick,, T. J. Schall, and , S. R. McColl. 1993. Expression of the cytokine RANTES in human rheumatoid synovial fibroblasts. Differential regulation of RANTES and interleukin-8 genes by inflammatory cytokines. J. Biol. Chem. 268: 5834– 5839.

99. Reape, T. J.,, K. Rayner,, C. D. Manning,, A. N. Gee,, M. S. Barnette,, K. G. Burnand, and , P. H. Groot. 1999. Expression and cellular localization of the CC chemokines PARC and ELC in human atherosclerotic plaques. Am. J. Pathol. 154: 365– 374.

100. Reynaud-Gaubert, M.,, V. Marin,, X. Thirion,, C. Farnarier,, P. Thomas,, M. Badier,, P. Bongrand,, R. Giudicelli, and , P. Fuentes. 2002. Upregulation of chemokines in bronchoalveolar lavage fluid as a predictive marker of post-transplant airway obliteration. J. Heart Lung Transplant. 21: 721– 730.

101. Richards, K. L., and , J. McCullough. 1984. A modified microchamber method for chemotaxis and chemokinesis. Immunol. Commun. 13: 49– 62.

102. Rimaniol, A. C.,, S. J. Till,, G. Garcia,, F. Capel,, V. Godot,, K. Balabanian,, I. Durand-Gasselin,, E. M. Varga,, G. Simonneau,, D. Emilie,, S. R. Durham, and , M. Humbert. 2003. The CX3C chemokine fractalkine in allergic asthma and rhinitis. J. Allergy Clin. Immunol. 112: 1139– 1146.

103. Riviere, C.,, F. Subra,, K. Cohen-Solal,, V. Cordette-Lagarde,, R. Letestu,, C. Auclair,, W. Vainchenker, and , F. Louache. 1999. Phenotypic and functional evidence for the expression of CXCR4 receptor during megakaryocytopoiesis. Blood 93: 1511– 1523.

104. Robinson, E.,, E. C. Keystone,, T. J. Schall,, N. Gillett, and , E. N. Fish. 1995. Chemokine expression in rheumatoid arthritis (RA): evidence of RANTES and macrophage inflammatory protein (MIP)-1 beta production by synovial T cells. Clin. Exp. Immunol. 101: 398– 407.

105. Robinson, L. A.,, C. Nataraj,, D. W. Thomas,, D. N. Howell,, R. Griffiths,, V. Bautch,, D. D. Patel,, L. Feng, and , T. M. Coffman. 2000. A role for fractalkine and its receptor (CX3CR1) in cardiac allograft rejection. J. Immunol. 165: 6067– 6072.

106. Rollins, B. J. 1997. Chemokines. Blood 90: 909– 928.

107. Ross, D. J.,, A. M. Cole,, D. Yoshioka,, A. K. Park,, J. A. Belperio,, H. Laks,, R. M. Strieter,, J. P. Lynch III,, B. Kubak,, A. Ardehali, and , T. Ganz. 2004. Increased bronchoalveolar lavage human beta-defensin type 2 in bronchiolitis obliterans syndrome after lung transplantation. Transplantation 78: 1222– 1224.

108. Rossi, D., and , A. Zlotnik. 2000. The biology of chemokines and their receptors. Annu. Rev. Immunol. 18: 217– 242.

109. Rot, A. 1993. Neutrophil attractant/activation protein-1 (interleukin-8) induces in vitro neutrophil migration by haptotactic mechanism. Eur. J. Immunol. 23: 303– 306.

110. Rothenberg, M. E. 2000. Chemokine knockout mice. Methods. Mol. Biol. 138: 253– 257.

111. Rothenberg, M. E.,, J. A. MacLean,, E. Pearlman,, A. D. Luster, and , P. Leder. 1997. Targeted disruption of the chemokine eotaxin partially reduces antigen-induced tissue eosinophilia. J. Exp. Med. 185: 785– 790.

112. Ruth, J. H.,, M. V. Volin,, G. K. Haines III,, D. C. Woodruff,, K. J. Katschke, Jr.,, J. M. Woods,, C. C. Park,, J. C. Morel, and , A. E. Koch. 2001. Fractalkine, a novel chemokine in rheumatoid arthritis and in rat adjuvant-induced arthritis. Arthritis Rheum. 44: 1568– 1581.

113. Salazar-Mather, T. P.,, J. S. Orange, and , C. A. Biron. 1998. Early murine cytomegalovirus (MCMV) infection induces liver natural killer (NK) cell inflammation and protection through macrophage inflammatory protein 1alpha (MIP-1alpha)-dependent pathways. J. Exp. Med. 187: 1– 14.

114. Sallusto, F.,, C. R. Mackay, and , A. Lanzavecchia. 2000. The role of chemokine receptors in primary, effector, and memory immune responses. Annu. Rev. Immunol. 18: 593– 620.

115. Sanchez-Madrid, F., and , M. A. del Pozo. 1999. Leukocyte polarization in cell migration and immune interactions. EMBO J. 18: 501– 511.

116. Servant, G.,, O. D. Weiner,, P. Herzmark,, T. Balla,, J. W. Sedat, and , H. R. Bourne. 2000. Polarization of chemoattractant receptor signaling during neutrophil chemotaxis. Science 287: 1037– 1040.

117. Shepherd, D. M.,, E. A. Dearstyne, and , N. I. Kerkvliet. 2000. The effects of TCDD on the activation of ovalbumin (OVA)-specific DO11.10 transgenic CD4(+) T cells in adoptively transferred mice. Toxicol. Sci. 56: 340– 350.

118. Shin, W. S.,, A. Szuba, and , S. G. Rockson. 2002. The role of chemokines in human cardiovascular pathology: enhanced biological insights. Atherosclerosis 160: 91– 102.

119. Simson, L., and , P. S. Foster. 2000. Chemokine and cytokine cooperativity: eosinophil migration in the asthmatic response. Immunol. Cell Biol. 78: 415– 422.

120. Snowden, N.,, A. Hajeer,, W. Thomson, and , B. Ollier. 1994. RANTES role in rheumatoid arthritis. Lancet 343: 547– 548.

121. Sozzani, S.,, P. Allavena,, G. D’Amico,, W. Luini,, G. Bianchi,, M. Kataura,, T. Imai,, O. Yoshie,, R. Bonecchi, and , A. Mantovani. 1998. Differential regulation of chemokine receptors during dendritic cell maturation: a model for their trafficking properties. J. Immunol. 161: 1083– 1086.

122. Szekanecz, Z.,, J. Kim, and , A. E. Koch. 2003. Chemokines and chemokine receptors in rheumatoid arthritis. Semin. Immunol. 15: 15– 21.

123. Taha, R. A.,, S. Laberge,, Q. Hamid, and , R. Olivenstein. 2001. Increased expression of the chemoattractant cytokines eotaxin, monocyte chemotactic protein-4, and interleukin-16 in induced sputum in asthmatic patients. Chest 120: 595– 601.

124. Tanaka, Y.,, D. H. Adams,, S. Hubscher,, H. Hirano,, U. Siebenlist, and , S. Shaw. 1993. T-cell adhesion induced by proteoglycan-immobilized cytokine MIP-1 beta. Nature 361: 79– 82.

125. Tanaka, Y.,, D. H. Adams, and , S. Shaw. 1993. Proteoglycans on endothelial cells present adhesion-inducing cytokines to leukocytes. Immunol. Today 14: 111– 115.

126. Tenscher, K.,, B. Metzner,, E. Schopf,, J. Norgauer, and , W. Czech. 1996. Recombinant human eotaxin induces oxygen radical production, Ca(2+)-mobilization, actin reorganization, and CD11b upregulation in human eosinophils via a pertussis toxin-sensitive heterotrimeric guanine nucleotide-binding protein. Blood 88: 3195– 3199.

127. Tesch, G. H.,, A. Schwarting,, K. Kinoshita,, H. Y. Lan,, B. J. Rollins, and , V. R. Kelley. 1999. Monocyte chemoattractant protein-1 promotes macrophage-mediated tubular injury, but not glomerular injury, in nephrotoxic serum nephritis. J. Clin. Investig. 103: 73– 80.

128. Traynor, T. R.,, W. A. Kuziel,, G. B. Toews, and , G. B. Huffnagle. 2000. CCR2 expression determines T1 versus T2 polarization during pulmonary Cryptococcus neoformans infection. J. Immunol. 164: 2021– 2027.

129. Tsien, R. Y. 1980. New calcium indicators and buffers with high selectivity against magnesium and protons: design, synthesis, and properties of prototype structures. Biochemistry 19: 2396– 2404.

130. Tsien, R. Y.,, T. J. Rink, and , M. Poenie. 1985. Measurement of cytosolic free Ca 2+ in individual small cells using fluorescence microscopy with dual excitation wavelengths. Cell Calcium 6: 145– 157.

131. van Es, S.,, D. Wessels,, D. R. Soll,, J. Borleis, and , P. N. Devreotes. 2001. Tortoise, a novel mitochondrial protein, is required for directional responses of Dictyostelium in chemotactic gradients. J. Cell Biol. 152: 621– 632.

132. Waldmann, T. A. 1991. Monoclonal antibodies in diagnosis and therapy. Science 252: 1657– 1662.

133. Wang, N.,, I. Tabas,, R. Winchester,, S. Ravalli,, L. E. Rabbani, and , A. Tall. 1996. Interleukin 8 is induced by cholesterol loading of macrophages and expressed by macrophage foam cells in human atheroma. J. Biol. Chem. 271: 8837– 8842.

134. Wank, S. A. 1998. G protein-coupled receptors in gastrointestinal physiology. I. CCK receptors: an exemplary family. Am. J. Physiol. 274: G607– G613.

135. Weiner, O. D.,, G. Servant,, M. D. Welch,, T. J. Mitchison,, J. W. Sedat, and , H. R. Bourne. 1999. Spatial control of actin polymerization during neutrophil chemotaxis. Nat. Cell. Biol. 1: 75– 81.

136. Weissleder, R., and , U. Mahmood. 2001. Molecular imaging. Radiology 219: 316– 333.

137. Wells, T. N.,, C. A. Power, and , A. E. Proudfoot. 1998. Definition, function and pathophysiological significance of chemokine receptors. Trends Pharmacol. Sci. 19: 376– 380.

138. Westlin, W. F.,, J. M. Kiely, and , M. A. Gimbrone, Jr. 1992. Interleukin-8 induces changes in human neutrophil actin conformation and distribution: relationship to inhibition of adhesion to cytokine-activated endothelium. J. Leukoc. Biol. 52: 43– 51.

139. Wilcox, J. N.,, N. A. Nelken,, S. R. Coughlin,, D. Gordon, and , T. J. Schall. 1994. Local expression of inflammatory cytokines in human atherosclerotic plaques. J. Atheroscler. Thromb. 1 (Suppl. 1): S10– S13.

140. Winter, P. M.,, A. M. Morawski,, S. D. Caruthers,, R. W. Fuhrhop,, H. Zhang,, T. A. Williams,, J. S. Allen,, E. K. Lacy,, J. D. Robertson,, G. M. Lanza, and , S. A. Wickline. 2003. Molecular imaging of angiogenesis in early-stage atherosclerosis with alpha(v)beta3-integrin-targeted nanoparticles. Circulation 108: 2270– 2274.

141. Xie, H.,, Y. C. Lim,, F. W. Luscinskas, and , A. H. Lichtman. 1999. Acquisition of selectin binding and peripheral homing properties by CD4(+) and CD8(+) T cells. J. Exp. Med. 189: 1765– 1776.

142. Ying, S., and , A. B. Kay. 2000. Chemokines in allergic asthma, p. 383– 401. In M. E. Rothenberg (ed.), Chemokines in Allergic Disease. Marcel Dekker AG, New York, N.Y.

143. Zhao, D. X.,, Y. Hu,, G. G. Miller,, A. D. Luster,, R. N. Mitchell, and , P. Libby. 2002. Differential expression of the IFN-gamma-inducible CXCR3-binding chemokines, IFN-inducible protein 10, monokine induced by IFN, and IFN-inducible T cell alpha chemoattractant in human cardiac allografts: association with cardiac allograft vasculopathy and acute rejection. J. Immunol. 169: 1556– 1560.

144. Zlotnik, A., and , O. Yoshie. 2000. Chemokines: a new classification system and their role in immunity. Immunity 12: 121– 127.

Tables

Chemokine and Chemokine Receptor Analysis

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TABLE 1

Chemokines and receptors

TABLE 1

Chemokines and receptors

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TABLE 2

Chemokines identified in human atherosclerotic lesions

TABLE 2

Chemokines identified in human atherosclerotic lesions

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TABLE 3

Chemokines and chemokine receptors with importance in RA based on human and animal studies ( 122 )

TABLE 3

Chemokines and chemokine receptors with importance in RA based on human and animal studies ( 122 )

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TABLE 4

Chemokines and chemokine receptors with importance in asthma based on human and animal studies ( 142 )

TABLE 4

Chemokines and chemokine receptors with importance in asthma based on human and animal studies ( 142 )

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TABLE 5

Chemokines and chemokine receptors with importance in transplantation based on human and animal studies ( 31 )

TABLE 5

Chemokines and chemokine receptors with importance in transplantation based on human and animal studies ( 31 )