Chapter 53 : Immunologic Methods for Diagnosis of Infections Caused by Diarrheagenic and

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The families and encompass a heterogeneous group of gram-negative organisms. The organisms chosen for discussion in this chapter are recognized as major public health concerns because of their significant contribution to morbidity and mortality in both children and adults in less developed and industrialized countries. Cytotoxicity is usually examined under the microscope or can be quantified by colorimetric methods. Positive samples should be retested in the presence of Shiga toxins (Stx)-specific antibodies to confirm the specificity of the cytotoxic effect. The optimal concentration of antigen should be determined by standard enzyme-linked immunosorbent assay (ELISA) checkerboard titration, coating plates with different amounts of antigen and testing them with known positive and negative control sera during the standardization of the assay. Diagnosis of acute enterotoxigenic (ETEC) infections requires the detection of labile toxin (LT), ST, or toxin genes in isolated strains. Typical enteropathogenic (EPEC) strains carry both a chromosomally encoded (the locus of enterocyte effacement (LEE), which mediates a complex signal transduction phenotype on the target epithelial cell, and the EPEC adherence factor plasmid (EAF), which encodes the principal EPEC adhesin.

Citation: Pasetti M, Nataro J, Levine M. 2006. Immunologic Methods for Diagnosis of Infections Caused by Diarrheagenic and , p 448-461. In Detrick B, Hamilton R, Folds J (ed), Manual of Molecular and Clinical Laboratory Immunology, 7th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815905.ch53
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