Chapter 54 : Serologic and Molecular Diagnosis of Infection and Eradication

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The CagA protein, which is highly immunogenic, is one of the most widely studied virulence factors of . The cagA gene is one of the genes in the cag pathogenicity island. A variety of techniques are available for the diagnosis of infection. In this chapter the tests are discussed generically rather than listed specifically. In an attempt to eliminate the need for venipuncture, diagnostic tests have been developed for the detection of IgG and IgA antibodies in saliva and urine. Studies using urine-based enzyme-linked immunosorbent assay (ELISA) for detection of IgG antibodies against have been conducted. The most important factor in using immunological methods for identifying VacA-specific antibodies by ELISA is the antigen preparation used to detect circulating antibodies. The availability of recombinant VacA protein will help to make the VacA ELISA more widely available. In summary, ELISAs for detection of -specific antibodies are sensitive, specific, and cost-effective in untreated individuals. The major limitation of such tests is the lack of a rapid, clear serologic response to eradication of bacterial infection. Molecular tests have been developed which preclude culture and allow direct, sensitive detection of and specific virulence factors thereof in a variety of biological specimens. Much work remains to be done to standardize molecular assays so that they can be applied generally to the diagnosis of infection and eradication.

Citation: Dunn B, Phadnis S. 2006. Serologic and Molecular Diagnosis of Infection and Eradication, p 462-467. In Detrick B, Hamilton R, Folds J (ed), Manual of Molecular and Clinical Laboratory Immunology, 7th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815905.ch54
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1. Alarcon, T.,, A. E. Vega,, D. Domingo,, M. J. Martinez, and , M. Lopez-Brea. 2003. Clarithromycin resistance among Helicobacter pylori strains isolated from children: prevalence and study of mechanism of resistance by PCR-restriction fragment length polymorphism analysis. J. Clin. Microbiol. 41: 486499.
2. Bergy, B.,, P. Marchildon,, J. Peacock, and , F. Megraud. 2003. What is the role of serology in assessing Helicobacter pylori eradication? Aliment. Pharmacol. Ther. 18: 635639.
3. Chisholm, S. A.,, E. L. Teare,, B. Patel, and , R. J. Owen. 2002. Determination of Helicobacter pylori VacA allelic types by single-step multiplex PCR. Lett. Appl. Microbiol. 35: 4246.
4. Crone, J., and , B. D. Gold. 2004. Helicobacter pylori infection in pediatrics. Helicobacter 9(Suppl. 1): 4956.
5. Graham, D. Y.,, A. R. Opekun,, F. Hammoud,, Y. Yamaoka,, R. Reddy,, M. S. Osato, and , H. M. El-Zimaity. 2003. Studies regarding the mechanism of false negative urea breath tests with proton pump inhibitors. Am. J. Gastroenterol. 98: 10051009.
6. Hao, Q.,, Y. Li,, Z. J. Zhang,, Y. Liu, and , H. Gao. 2004. New mutation points in 23S rRNA gene associated with Helicobacter pylori resistance to clarithromycin in northeast China. World J. Gastroenterol. 10: 10751077.
7. Kabir, S. 2004. Detection of Helicobacter pylori DNA in feces and saliva by polymerase chain reaction: a review. Helicobacter 9: 115123.
8. Kobayashi, D.,, Y. Eishi,, T. Ohkusa,, T. Ishige,, T. Suzuki,, J. Minami,, T. Yamada,, T. Takizawa, and , M. Koike. 2002. Gastric mucosal density of Helicobacter pylori estimated by real-time PCR compared with results of urea breath test and histological grading. J. Med. Microbiol. 51: 305311.
9. Krah, A.,, S. Miehlke,, K. P. Pleissner,, U. Zimny-Arndt,, C. Kirsch,, N. Lehn,, T. F. Meyer,, P. R. Jungblut, and , T. Aebischer. 2004. Identification of candidate antigens for serologic detection of Helicobacter pylori-infected patients with gastric carcinoma. Int. J. Cancer 108: 456463.
10. Lehmann, F. S., and , C. Beglinger. 2003. Current role of Helicobacter pylori stool tests. Digestion 68: 119123.
11. Lehours, P.,, A. Ruskone-Fourmestraux,, A. Lavergne,, A. Cantet, and , F. Megraud. 2003. Which test to use to detect Helicobacter pylori infection in patients with low grade gastric mucosa-associated lymphoid tissue lymphoma? Am. J. Gastroenterol. 98: 291295.
12. Leodolter, A.,, U. Peitz,, M. P. Ebert,, K. Agha-Amiri, and , P. Malfertheiner. 2002. Comparison of two enzyme immunoassays for the assessment of Helicobacter pylori status in stool specimens after eradication therapy. Am. J. Gastroenterol. 97: 16821686.
13. Leodolter, A.,, D. Vaira,, F. Bazzoli,, K. Schutze,, A. Hirschl,, F. Megraud, and , P. Malfertheiner. 2003. European multicentre validation trial of two new non-invasive tests for the detection of Helicobacter pylori antibodies: urine-based ELISA and rapid urine test. Aliment. Pharmacol. Ther. 18: 927931.
14. Makristathis, A.,, A. M. Hirschl,, P. Lehours, and , F. Megraud. 2004. Diagnosis of Helicobacter pylori infection. Helicobacter 9(Suppl. 1): 714.
15. Malfertheiner, P.,, F. Megraud,, C. O’Morain, and the European Helicobacter pylori Study Group (EHPSG). 2002. Current concepts in the management of Helicobacter pylori infection—the Maastricht 2-2000 Consensus Report. Aliment. Pharmacol. Ther. 16: 167180.
16. Matsumura, M.,, Y. Hikiba,, K. Ogura,, G. Togo,, I. Tsukuda,, K. Ushikawa,, Y. Shiratori, and , M. Omata. 2001. Rapid detection of mutations in the 23S rRNA gene of Helicobacter pylori that confers resistance to clarithromycin treatment to the bacterium. J. Clin. Microbiol. 39: 691695.
17. Megraud, F. 2001. Impact of Helicobacter pylori virulence in the outcome of gastroduodenal diseases: lessons from the microbiologist. Dig. Dis. 19: 99103.
18. Monteiro, L.,, B. Bergey,, N. Gras, and , F. Megraud. 2002. Evaluation of the performance of the Helico Blot 2.1 as a tool to investigate the virulence properties of Helicobacter pylori. Clin. Microbiol. Infect. 8: 676679.
19. National Institutes of Health Consensus Conference. 1994. Helicobacter pylori in peptic ulcer disease. JAMA 272: 6569.
20. Oleastro, M.,, A. Menard,, A. Santos,, H. Lamouliatte,, L. Monteiro,, P. Barthelemy, and , F. Megraud. 2003. Realtime PCR assay for rapid and accurate detection of point mutations conferring resistance to clarithromycin in Helicobacter pylori. J. Clin. Microbiol. 41: 397402.
21. Park, C. Y.,, Y. K. Cho,, T. Kodama,, H. M. El-Zimaity,, M. S. Osato,, D. Y. Graham, and , Y. Yamaoka. 2002. New serological assay for detection of putative Helicobacter pylori virulence factors. J. Clin. Microbiol. 40: 47534756.
22. Perez-Perez, G. I.,, R. M. Peek,, J. C. Atherton,, M. J. Blaser, and , T. L. Cover. 1999. Detection of anti-VacA antibody response in serum and gastric juice samples using types 1/ma and s2/m2 Helicobacter pylori VacA antigens. Clin. Diagn. Lab. Immunol. 6: 489493.
23. Perri, F.,, G. Manes,, M. Neri,, D. Vaira, and , G. Nardone. 2002. Helicobacter pylori antigen stool test and 13C-urea breath test in patients after eradication treatments. Am. J. Gastroenterol. 97: 27562762.
24. Rautelin, H.,, P. Lehours, and , F. Megraud. 2003. Diagnosis of Helicobacter pylori infection. Helicobacter 8(Suppl. 1): 1320.
25. Ribeiro, M. L.,, M. M. Gerrits,, Y. H. Benvengo,, M. Berning,, A. P. Godoy,, E. J. Kuipers,, S. Mendonca,, A. H. van Vliet,, J. Pedrazzoli, Jr., and , J. G. Kusters. 2004. Detection of high-level tetracycline resistance in clinical isolates of Helicobacter pylori using PCR-RFLP. FEMS Immunol. Med. Microbiol. 40: 5761.
26. Segal, E. D.,, J. Cha,, J. Lo,, S. Falkow, and , L. S. Tompkins. 1999. Altered states: involvement of phosphorylated CagA in the induction of host cellular growth changes by Helicobacter pylori. Proc. Natl. Acad. Sci. USA 96: 1455914564.
27. Shimizu, T.,, Y. Yarita,, H. Haruna,, K. Kaneko,, Y. Yamashiro,, R. Gupta,, A. Anazawa, and , K. Suzuki. 2003. Urine-based enzyme-linked immunosorbent assay for the detection of Helicobacter pylori antibodies in children. J. Paediatr. Child Health 39: 606610.
28. Sicinschi, L. A.,, P. Correa,, L. E. Bravo, and , B. G. Schneider. 2003. A positive assay for identification of cagA negative strains of Helicobacter pylori. J. Microbiol. Methods 55: 625633.
29. Sokic-Milutinovic, T.,, T. Wex,, V. Todorovic,, T. Milosavljevic, and , P. Malfertheiner. 2004. Anti-CagA and Anti-VacA antibodies in Helicobacter pylori-infected patients with and without peptic ulcer disease in Serbia and Montenegro. Scand. J. Gastroenterol. 3: 222226.


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Diagnostic tests for detection of

Citation: Dunn B, Phadnis S. 2006. Serologic and Molecular Diagnosis of Infection and Eradication, p 462-467. In Detrick B, Hamilton R, Folds J (ed), Manual of Molecular and Clinical Laboratory Immunology, 7th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815905.ch54

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