Rapid Methods for the Detection of Foodborne Pathogens: Current and Next-Generation Technologies

Peter Feng

doi:10.1128/9781555815912.ch43

Chapter 43 : Rapid Methods for the Detection of Foodborne Pathogens: Current and Next-Generation Technologies

Author: Peter Feng¹

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Affiliations: 1: Division of Microbiological Studies, U.S. Food and Drug Administration, College Park, MD 20740-3835

Content Type: Reference

Publication Year: 2007

Category: Applied and Industrial Microbiology; Food Microbiology

Book DOI: 10.1128/9781555815912

Chapter DOI: 10.1128/9781555815912.ch43

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Abstract:

The history of biotechnology reveals that scientific discoveries lead to technological advances and these are usually followed by a phase of research and development to find applications for the technologies. The authors are currently in another application phase, where new technologies such as real-time PCR (rtPCR), DNA microarrays, and biosensors are providing very sophisticated tools for use in diagnostics. This chapter therefore includes discussions on these "next-generation technologies," which will have an impact on the way one can test for pathogens and toxins in foods. All identification assays require a pure culture of the unknown bacteria, which is then identified most often by its biochemical characteristics. It is a lengthy, labor-intensive, and media-consuming process. The introduction of miniaturized biochemical kits, which provide a quick biochemical profile of bacteria at a great savings in cost, labor, and time, has simplified the process of bacterial identification and continues to be important in regulatory testing of foods. Many of the new methods use next-generation technologies such as rtPCR, biosensors, and DNA chips, which may be more rapid, more sensitive, and capable of multitarget testing and hence are well suited for use in screening large numbers of samples in compliance or food security surveillance programs. Furthermore, comparative evaluation by with standard methods or validation of rapid methods is critical to document their efficacy in detecting foodborne pathogens and toxins.

Citation: Feng P. 2007. Rapid Methods for the Detection of Foodborne Pathogens: Current and Next-Generation Technologies, p 911-934. In Doyle M, Beuchat L (ed), Food Microbiology: Fundamentals and Frontiers, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555815912.ch43

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Rapid Methods for the Detection of Foodborne Pathogens: Current and Next-Generation Technologies

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Figure 43.1

History of biotechnology. Modified from reference 29 .

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Figure 43.1

History of biotechnology. Modified from reference 29 .

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Figure 43.2

Conventional microbiological analysis of foods.

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Figure 43.2

Conventional microbiological analysis of foods.

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Tables

Rapid Methods for the Detection of Foodborne Pathogens: Current and Next-Generation Technologies

/content/10.1128/9781555815912.ch43.ch43tab01

Table 43.1

Partial list of miniaturized and automated identification kits for bacteria a

Table 43.1

Partial list of miniaturized and automated identification kits for bacteria a

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Table 43.2

Partial list of commercially available assays and specialty substrate media for detection of foodborne bacteria a

Table 43.2

Partial list of commercially available assays and specialty substrate media for detection of foodborne bacteria a

/content/10.1128/9781555815912.ch43.ch43tab03

Table 43.3

Partial list of commercially available nucleic acid-based assays for detection of foodborne bacterial pathogens a

Table 43.3

Partial list of commercially available nucleic acid-based assays for detection of foodborne bacterial pathogens a

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Table 43.4

Partial list of commercially available antibody-based assays for the detection of foodborne pathogens a

Table 43.4

Partial list of commercially available antibody-based assays for the detection of foodborne pathogens a

/content/10.1128/9781555815912.ch43.ch43tab05

Table 43.5

Partial listing of rapid methods for the detection of bacterial toxins a

Table 43.5

Partial listing of rapid methods for the detection of bacterial toxins a

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Table 43.6

Approximate detection sensitivity of various formats

Table 43.6

Approximate detection sensitivity of various formats