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Chapter 12 : Gel Electrophoresis

Editors: Helen Kreuzer1, Adrianne Massey2
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Affiliations: 1: Pacific Northwest National Laboratory, Richland,Washington; 2: A. Massey & Associates, Chapel Hill, North Carolina
Content Type: Textbook
Publication Year: 2008

Category: Microbial Genetics and Molecular Biology; Applied and Industrial Microbiology

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Gel Electrophoresis, Page 1 of 2

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Abstract:

is a reading and paper activity that introduces electrophoresis. Students should already be familiar with the activity of restriction enzymes through the activity DNA Scissors. This lesson provides enough background for students to continue with activities (hybridization analysis, DNA sequencing, DNA fingerprinting) that require them to know something about the process. It is also an excellent introduction if you plan to conduct electrophoresis in your classroom. The activity is self-explanatory. Students can read the handout and do the exercises. The students should be shown as much real material (or pictures) as one can: gel boxes, power supplies, an old gel, or a photograph of one. Relate the items to their activity and reading.

Citation: Kreuzer H, Massey A. 2008. Gel Electrophoresis, p 225-229. In Molecular Biology and Biotechnology: A Guide for Teachers, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816100.ch12

Key Concept Ranking

Agarose Gel Electrophoresis
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DNA Restriction Enzymes
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Gel Electrophoresis
Citation: Kreuzer H, Massey A. 2008. Gel Electrophoresis, p 225-229. In Molecular Biology and Biotechnology: A Guide for Teachers, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816100.ch12
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Citation: Kreuzer H, Massey A. 2008. Gel Electrophoresis, p 225-229. In Molecular Biology and Biotechnology: A Guide for Teachers, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816100.ch12
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Figure 12.1

Casting an agarose gel. (A) To make a gel, hot liquid agarose solution is poured into a casting tray (any shallow container) and the comb is put in place. (B) After the agarose cools and hardens, the comb is removed, leaving behind pits in the gel called sample wells. Samples are loaded into the wells prior to electrophoresis.

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10.1128/9781555816100/fig12-1.gif
Image of Figure 12.1
Figure 12.1

Casting an agarose gel. (A) To make a gel, hot liquid agarose solution is poured into a casting tray (any shallow container) and the comb is put in place. (B) After the agarose cools and hardens, the comb is removed, leaving behind pits in the gel called sample wells. Samples are loaded into the wells prior to electrophoresis.

Citation: Kreuzer H, Massey A. 2008. Gel Electrophoresis, p 225-229. In Molecular Biology and Biotechnology: A Guide for Teachers, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816100.ch12
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Figure 12.2

In electrophoresis, the gel is placed in a tank of salt solution, and an electric current is applied. The DNA migrates toward the positive pole.

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10.1128/9781555816100/fig12-2.gif
Image of Figure 12.2
Figure 12.2

In electrophoresis, the gel is placed in a tank of salt solution, and an electric current is applied. The DNA migrates toward the positive pole.

Citation: Kreuzer H, Massey A. 2008. Gel Electrophoresis, p 225-229. In Molecular Biology and Biotechnology: A Guide for Teachers, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816100.ch12
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Figure 12.3

A scientist is using a micropipette to load a DNA sample into an agarose gel. The gel is in an electrophoresis chamber full of buffer. The power supply for the chamber is on the laboratory bench behind the chamber.

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Image of Figure 12.3
Figure 12.3

A scientist is using a micropipette to load a DNA sample into an agarose gel. The gel is in an electrophoresis chamber full of buffer. The power supply for the chamber is on the laboratory bench behind the chamber.

Citation: Kreuzer H, Massey A. 2008. Gel Electrophoresis, p 225-229. In Molecular Biology and Biotechnology: A Guide for Teachers, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816100.ch12
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Figure 12.4

In electrophoresis races, the small DNA always wins!

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Image of Figure 12.4
Figure 12.4

In electrophoresis races, the small DNA always wins!

Citation: Kreuzer H, Massey A. 2008. Gel Electrophoresis, p 225-229. In Molecular Biology and Biotechnology: A Guide for Teachers, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816100.ch12
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/content/10.1128/9781555816100.chap12a.fig12-5
Figure 12.5

Gel electrophoresis is used to separate products of restriction digestion. (A) Restriction map, with fragment sizes in base pairs. (B) View of gel after electrophoresis.

10.1128/9781555816100/fig12-5_thmb.gif
10.1128/9781555816100/fig12-5.gif
Image of Figure 12.5
Figure 12.5

Gel electrophoresis is used to separate products of restriction digestion. (A) Restriction map, with fragment sizes in base pairs. (B) View of gel after electrophoresis.

Citation: Kreuzer H, Massey A. 2008. Gel Electrophoresis, p 225-229. In Molecular Biology and Biotechnology: A Guide for Teachers, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816100.ch12
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References

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