Chapter 10 : Prevention and Control of Laboratory-Acquired Infections

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This chapter talks about laboratory biosafety and its goals that include prevention of laboratory-acquired infections in workers and accidental releases of live agents which can potentially endanger and have severe negative impact on humans, animals, and plants. Laboratory safety involves all aspects of the laboratory cycle, starting from before microorganisms arrive in the facility and continuing through the training of personnel, the establishment and monitoring of safe working practices, the proper use of reagents, materials, and equipment, the safe storage and transport of agents, and ultimately the terminal sterilization and destruction of microorganisms. Attitudes and work habits are considered to be important contributing factors to laboratory accidents according to a matched case-control study. Scalpels, needles, broken glass, and other sharps are commonly associated with wound injuries and laboratory-acquired infections. Hand washing is a useful technique to stop the transmission of microorganisms and acquisition of infection in medical laboratories. Gloves can provide an important barrier within the laboratory, provided that they are used appropriately. Immunization provides protection against some laboratory acquired infectious diseases but should be considered secondary to mental alertness and good laboratory practices. Finally, personal protective equipment, including M95 respirators, should be considered for additional protection.

Citation: Noble M. 2011. Prevention and Control of Laboratory-Acquired Infections, p 132-142. In Versalovic J, Carroll K, Funke G, Jorgensen J, Landry M, Warnock D (ed), Manual of Clinical Microbiology, 10th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816728.ch10
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Generic image for table

Risk-based classification of microorganisms

Based on reference . This table is presented as a guide only. It should not be considered complete or consistent within all jurisdictions. Specific national requirements may differ from the information presented in this table.

Citation: Noble M. 2011. Prevention and Control of Laboratory-Acquired Infections, p 132-142. In Versalovic J, Carroll K, Funke G, Jorgensen J, Landry M, Warnock D (ed), Manual of Clinical Microbiology, 10th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816728.ch10
Generic image for table

Laboratory design requirements for biosafety level 2 laboratories

Adapted from references and .

Citation: Noble M. 2011. Prevention and Control of Laboratory-Acquired Infections, p 132-142. In Versalovic J, Carroll K, Funke G, Jorgensen J, Landry M, Warnock D (ed), Manual of Clinical Microbiology, 10th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816728.ch10
Generic image for table

Standard microbiology practices for all laboratories (biosafety level 2 and higher)

Adapted from references and .

Citation: Noble M. 2011. Prevention and Control of Laboratory-Acquired Infections, p 132-142. In Versalovic J, Carroll K, Funke G, Jorgensen J, Landry M, Warnock D (ed), Manual of Clinical Microbiology, 10th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816728.ch10
Generic image for table

Laboratory safety audits required by ISO 15190:2003

See reference .

Citation: Noble M. 2011. Prevention and Control of Laboratory-Acquired Infections, p 132-142. In Versalovic J, Carroll K, Funke G, Jorgensen J, Landry M, Warnock D (ed), Manual of Clinical Microbiology, 10th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816728.ch10
Generic image for table

Electronic information sources and resources

Citation: Noble M. 2011. Prevention and Control of Laboratory-Acquired Infections, p 132-142. In Versalovic J, Carroll K, Funke G, Jorgensen J, Landry M, Warnock D (ed), Manual of Clinical Microbiology, 10th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816728.ch10

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