Streptococcus

Barbara Spellerberg; Claudia Brandt

doi:10.1128/9781555816728.ch20

Chapter 20 : Streptococcus

Authors: Barbara Spellerberg, Claudia Brandt

Content Type: Reference

Publication Year: 2011

Category: Clinical Microbiology

Book DOI: 10.1128/9781555816728

Chapter DOI: 10.1128/9781555816728.ch20

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Abstract:

This chapter presents the information and the identification schemes which adhere in many aspects to the phenotypic classification system. In a study of the genus Streptococcus based on sequence comparisons of small-subunit (16S) rRNA genes, five species groups of viridans group streptococci were demonstrated in addition to the pyogenic group (beta-hemolytic, large-colony formers. Streptococci can cause infections in humans and in many different animal species including mammals and fish. Reflecting the ongoing changes in the epidemiology of group B streptococcal disease, the highest attack rates were observed in patients less than 1 year and adults greater than 65 years of age. The predominant reservoir for S. dysgalactiae subsp. equisimilis strains is the human host, and transmission usually occurs among humans. A rapid method for the detection of S.pyogenes in pharyngeal specimens is based on a single-stranded chemiluminescent nucleic acid probe assay to identify specific rRNA sequences. It is important to distinguish Streptococcus from Enterococcus prior to L-pyrrolidonyl-β-naphthylamide (PYR) testing, and strains of other related genera may be PYR positive (including the genera Abiotrophia, Aerococcus, Enterococcus, Gemella, and Lactococcus). The VP test can be performed for the identification of beta-hemolytic streptococci. In the majority of cases, typing of streptococci has no immediate clinical or therapeutic consequences. It is most often performed by reference laboratories for the purposes of epidemiologic studies and the evaluation of vaccine efficacy. Determination of streptococcal antibodies is indicated for the diagnosis of poststreptococcal disease.

Citation: Spellerberg B, Brandt C. 2011. Streptococcus, p 331-349. In Versalovic J, Carroll K, Funke G, Jorgensen J, Landry M, Warnock D (ed), Manual of Clinical Microbiology, 10th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816728.ch20

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Streptococcus

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FIGURE 1

Colony morphology of selected streptococci. (A) S. pyogenes strain ATCC12344; (B) clinical isolate of S. agalactiae; (C) clinical isolate of S. dysgalactiae subsp. equisimilis (Lancefield group G); (D) mixed culture of S. anginosus (ATCC12395, open arrow) and clinical isolate of S. dysgalactiae subsp. equisimilis (closed arrow) (note the difference in colony size); (E) clinical isolate of S. pneumoniae (note the central depression of the colonies); (F) clinical isolate of S. pneumoniae (note the mucoid appearance of colonies).

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FIGURE 1

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FIGURE 2

CAMP factor test. An arrowhead-shaped zone of hemolysis in the zone of the S. aureus beta-hemolysin is shown. (A) Clinical isolate of a weakly beta-hemolytic S. agalactiae strain. (B) Beta-hemolytic S. agalactiae strain O90R. (C) Nonhemolytic S. agalactiae strain R268.

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FIGURE 2

References

/content/book/10.1128/9781555816728.chap20

1. Arbique, J. C.,, C. Poyart,, P. Trieu-Cuot,, G. Quesne,, M. D. G. S. Carvalho,, A. G. Steigerwalt,, R. E. Morey,, D. Jackson,, R. J. Davidson,, and R. R. Facklam. 2004. Accuracy of phenotypic and genotypic testing for identification of Streptococcus pneumoniae and description of Streptococcus pseudopneumoniae sp. nov. J. Clin. Microbiol. 42: 4686– 4696.

2. Austrian, R. 1976. The quellung reaction, a neglected microbiologic technique. Mt. Sinai J. Med. 43: 699– 709.

3. Ayoub, E. M.,, B. Nelson,, S. T. Shulman,, D. J. Barrett,, J. D. Campbell,, G. Armstrong,, J. Lovejoy,, G. H. Angoff,, and S. Rockenmacher. 2003. Group A streptococcal antibodies in subjects with or without rheumatic fever in areas with high or low incidences of rheumatic fever. Clin. Diagn. Lab. Immunol. 10: 886– 890.

4. Barnham, M.,, T. J. Thornton,, and K. Lange. 1983. Nephritis caused by Streptococcus zooepidemicus (Lancefield group C). Lancet i: 945– 948.

5. Beall, B.,, R. R. Facklam,, J. A. Elliott,, A. R. Franklin,, T. Hoenes,, D. Jackson,, L. Laclaire,, T. Thompson,, and R. Viswanathan. 1998. Streptococcal emm types associated with T-agglutination types and the use of conserved emm gene restriction fragment patterns for subtyping group A streptococci. J. Med. Microbiol. 47: 893– 898.

6. Beck, M.,, R. Frodl,, and G. Funke. 2008. Comprehensive study of strains previously designated Streptococcus bovis consecutively isolated from human blood cultures and emended description of Streptococcus gallolyticus and Streptococcus infantarius subsp. coli. J. Clin. Microbiol. 46: 2966– 2972.

7. Beighton, D.,, J. M. Hardie,, and R. A. Whiley. 1991. A scheme for the identification of viridans streptococci. J. Med. Microbiol. 35: 367– 372.

8. Bekal, S.,, C. Gaudreau,, R. A. Laurence,, E. Simoneau,, and L. Raynal. 2006. Streptococcus pseudoporcinus sp. nov., a novel species isolated from the genitourinary tract of women. J. Clin. Microbiol. 44: 2584– 2586.

9. Benson, J. A.,, and P. Ferrieri. 2001. Rapid pulsed-field gel electrophoresis method for group B streptococcus isolates. J. Clin. Microbiol. 39: 3006– 3008.

10. Benson, J. A.,, A. E. Flores,, C. J. Baker,, S. L. Hillier,, and P. Ferrieri. 2002. Improved methods for typing nontypeable isolates of group B streptococci. Int. J. Med. Microbiol. 292: 37– 42.

11. Bergeron, M. G.,, D. Ke,, C. Menard,, F. J. Picard,, M. Gagnon,, M. Bernier,, M. Ouellette,, P. H. Roy,, S. Marcoux,, and W. D. Fraser. 2000. Rapid detection of group B streptococci in pregnant women at delivery. N. Engl. J. Med. 343: 175– 179.

12. Bisno, A. L. 2001. Acute pharyngitis. N. Engl. J. Med. 344: 205– 211.

13. Bisno, A. L.,, M. A. Gerber,, J. M. Gwaltney, Jr.,, E. L. Kaplan,, R. H. Schwartz, et al. 2002. Practice guidelines for the diagnosis and management of group A streptococcal pharyngitis. Clin. Infect. Dis. 35: 113– 125.

14. Block, T.,, E. Munson,, A. Culver,, K. Vaughan,, and J. E. Hryciuk. 2008. Comparison of carrot broth- and selective Todd-Hewitt broth-enhanced PCR protocols for real-time detection of Streptococcus agalactiae in prenatal vaginal/anorectal specimens. J. Clin. Microbiol. 46: 3615– 3620.

15. Bochud, P. Y.,, T. Calandra,, and P. Francioli. 1994. Bacteremia due to viridans streptococci in neutropenic patients: a review. Am. J. Med. 97: 256– 264.

16. Brandt, C. M.,, G. Haase,, N. Schnitzler,, R. Zbinden,, and R. Lutticken. 1999. Characterization of blood culture isolates of Streptococcus dysgalactiae subsp. equisimilis possessing Lancefield’s group A antigen. J. Clin. Microbiol. 37: 4194– 4197.

17. Brandt, C. M.,, and B. Spellerberg. 2009. Human infections due to Streptococcus dysgalactiae subspecies equisimilis. Clin. Infect. Dis. 49: 766– 772.

18. Carvalho Mda, G.,, M. L. Tondella,, K. McCaustland,, L. Weidlich,, L. McGee,, L. W. Mayer,, A. Steigerwalt,, M. Whaley,, R. R. Facklam,, B. Fields,, G. Carlone,, E. W. Ades,, R. Dagan,, and J. S. Sampson. 2007. Evaluation and improvement of real-time PCR assays targeting lytA, ply, and psaA genes for detection of pneumococcal DNA. J. Clin. Microbiol. 45: 2460– 2466.

19. Caufield, P. W.,, G. R. Cutter,, and A. P. Dasanayake. 1993. Initial acquisition of mutans streptococci by infants: evidence for a discrete window of infectivity. J. Dent. Res. 72: 37– 45.

20. Centers for Disease Control and Prevention. 2008. Invasive pneumococcal disease in children 5 years after conjugate vaccine introduction—eight states, 1998-2005. MMWR Morb. Mortal. Wkly. Rep. 57: 144– 148.

21. Chapin, K. C.,, P. Blake,, and C. D. Wilson. 2002. Performance characteristics and utilization of rapid antigen test, DNA probe, and culture for detection of group A streptococci in an acute care clinic. J. Clin. Microbiol. 40: 4207– 4210.

22. Chuard, C.,, and L. B. Reller. 1998. Bile-esculin test for presumptive identification of enterococci and streptococci: effects of bile concentration, inoculation technique, and incubation time. J. Clin. Microbiol. 36: 1135– 1136.

23. Cunningham, M. W. 2000. Pathogenesis of group A streptococcal infections. Clin. Microbiol. Rev. 13: 470– 511.

24. Dahesh, S.,, M. E. Hensler,, N. M. Van Sorge,, R. E. Gertz, Jr.,, S. Schrag,, V. Nizet,, and B. W. Beall. 2008. Point mutation in the group B streptococcal pbp2x gene conferring decreased susceptibility to beta-lactam antibiotics. Antimicrob. Agents Chemother. 52: 2915– 2918.

25. Davidson, R.,, R. Cavalcanti,, J. L. Brunton,, D. J. Bast,, J. C. de Azavedo,, P. Kibsey,, C. Fleming,, and D. E. Low. 2002. Resistance to levofloxacin and failure of treatment of pneumococcal pneumonia. N. Engl. J. Med. 346: 747– 750.

26. Davies, H. D.,, A. McGeer,, B. Schwartz,, K. Green,, D. Cann,, A. E. Simor,, D. E. Low, et al. 1996. Invasive group A streptococcal infections in Ontario, Canada. N. Engl. J. Med. 335: 547– 554.

27. Davies, H. D.,, M. A. Miller,, S. Faro,, D. Gregson,, S. C. Kehl,, and J. A. Jordan. 2004. Multicenter study of a rapid molecular-based assay for the diagnosis of group B Streptococcus colonization in pregnant women. Clin. Infect. Dis. 39: 1129– 1135.

28. Denys, G. A.,, and R. B. Carey. 1992. Identification of Streptococcus pneumoniae with a DNA probe. J. Clin. Microbiol. 30: 2725– 2727.

29. Desjardins, M.,, K. L. Delgaty,, K. Ramotar,, C. Seetaram,, and B. Toye. 2004. Prevalence and mechanisms of erythromycin resistance in group A and group B Streptococcus: implications for reporting susceptibility results. J. Clin. Microbiol. 42: 5620– 5623.

30. Devriese, L. A.,, P. Vandamme,, B. Pot,, M. Vanrobaeys,, K. Kersters,, and F. Haesebrouck. 1998. Differentiation between Streptococcus gallolyticus strains of human clinical and veterinary origins and Streptococcus bovis strains from the intestinal tracts of ruminants. J. Clin. Microbiol. 36: 3520– 3523.

31. Doern, G. V.,, M. J. Ferraro,, A. B. Brueggemann,, and K. L. Ruoff. 1996. Emergence of high rates of antimicrobial resistance among viridans group streptococci in the United States. Antimicrob. Agents Chemother. 40: 891– 894.

32. Dowell, S. F.,, R. L. Garman,, G. Liu,, O. S. Levine,, and Y. H. Yang. 2001. Evaluation of Binax NOW, an assay for the detection of pneumococcal antigen in urine samples, performed among pediatric patients. Clin. Infect. Dis. 32: 824– 825.

33. Efstratiou, A. 2000. Group A streptococci in the 1990s. J. Antimicrob. Chemother. 45( Suppl.): 3– 12.

34. Enright, M. C.,, and B. G. Spratt. 1999. Multilocus sequence typing. Trends Microbiol. 7: 482– 487.

35. Enright, M. C.,, B. G. Spratt,, A. Kalia,, J. H. Cross,, and D. E. Bessen. 2001. Multilocus sequence typing of Streptococcus pyogenes and the relationships between emm type and clone. Infect. Immun. 69: 2416– 2427.

36. Facklam, R. 2002. What happened to the streptococci: overview of taxonomic and nomenclature changes. Clin. Microbiol. Rev. 15: 613– 630.

37. Facklam, R.,, J. Elliott,, N. Pigott,, and A. R. Franklin. 1995. Identification of Streptococcus porcinus from human sources. J. Clin. Microbiol. 33: 385– 388.

38. Facklam, R.,, J. Elliott,, L. Shewmaker,, and A. Reingold. 2005. Identification and characterization of sporadic isolates of Streptococcus iniae isolated from humans. J. Clin. Microbiol. 43: 933– 937.

39. Facklam, R. R.,, D. R. Martin,, M. Lovgren,, D. R. Johnson,, A. Efstratiou,, T. A. Thompson,, S. Gowan,, P. Kriz,, G. J. Tyrrell,, E. Kaplan,, and B. Beall. 2002. Extension of the Lancefield classification for group A streptococci by addition of 22 new M protein gene sequence types from clinical isolates: emm103 to emm124. Clin. Infect. Dis. 34: 28– 38.

40. Facklam, R. R. 1987. Specificity study of kits for detection of group A streptococci directly from throat swabs. J. Clin. Microbiol. 25: 504– 508.

40a. Facklam, R. R.,, and J. A. Washington II,. 1991. Streptococcus and related catalase-negative gram-positive cocci, p. 238– 257. In A. Balows,, W. J. Hausler, Jr.,, K. L. Herrmann,, H. D. Isenberg,, and H. J. Shadomy (ed.), Manual of Clinical Microbiology, 5th ed. American Society for Microbiology, Washington, DC.

41. Farley, M. 1995. Group B streptococcal infection in older patients. Spectrum of disease and management strategies. Drugs Aging 6: 293– 300.

42. Fischetti, V. A. 1989. Streptococcal M protein: molecular design and biological behavior. Clin. Microbiol. Rev. 2: 285– 314.

43. Gavin, P. J.,, R. B. Thomson, Jr.,, S. J. Horng,, and R. Yogev. 2003. Neonatal sepsis caused by Streptococcus bovis variant (biotype II/2): report of a case and review. J. Clin. Microbiol. 41: 3433– 3435.

44. Gavino, M.,, and E. Wang. 2007. A comparison of a new rapid real-time polymerase chain reaction system to traditional culture in determining group B streptococcus colonization. Am. J. Obstet. Gynecol. 197: 388.e1-4.

45. Gerber, M. A.,, L. L. Wright,, and M. F. Randolph. 1987. Streptozyme test for antibodies to group A streptococcal antigens. Pediatr. Infect. Dis. J. 6: 36– 40.

46. Glazunova, O. O.,, D. Raoult,, and V. Roux. 2006. Streptococcus massiliensis sp. nov., isolated from a patient blood culture. Int. J. Syst. Evol. Microbiol. 56: 1127– 1131.

47. Gorm Jensen, T.,, H. Bossen Konradsen,, and B. Bruun. 1999. Evaluation of the Rapid ID 32 Strep system. Clin. Microbiol. Infect. 5: 417– 423.

48. Greenberg, D.,, A. Broides,, I. Blancovich,, N. Peled,, N. Givon-Lavi,, and R. Dagan. 2004. Relative importance of nasopharyngeal versus oropharyngeal sampling for isolation of Streptococcus pneumoniae and Haemophilus influenzae from healthy and sick individuals varies with age. J. Clin. Microbiol. 42: 4604– 4609.

49. Gutierrez, F.,, M. Masia,, J. C. Rodriguez,, A. Ayelo,, B. Soldan,, L. Cebrian,, C. Mirete,, G. Royo,, and A. M. Hidalgo. 2003. Evaluation of the immunochromatographic Binax NOW assay for detection of Streptococcus pneumoniae urinary antigen in a prospective study of community-acquired pneumonia in Spain. Clin. Infect. Dis. 36: 286– 292.

50. Gygax, S. E.,, J. A. Schuyler,, L. E. Kimmel,, J. P. Trama,, E. Mordechai,, and M. E. Adelson. 2006. Erythromycin and clindamycin resistance in group B streptococcal clinical isolates. Antimicrob. Agents Chemother. 50: 1875– 1877.

51. Haanpera, M.,, J. Jalava,, P. Huovinen,, O. Meurman,, and K. Rantakokko-Jalava. 2007. Identification of alpha-hemolytic streptococci by pyrosequencing the 16S rRNA gene and by use of VITEK 2. J. Clin. Microbiol. 45: 762– 770.

52. Haidan, A.,, S. R. Talay,, M. Rohde,, K. S. Sriprakash,, B. J. Currie,, and G. S. Chhatwal. 2000. Pharyngeal carriage of group C and group G streptococci and acute rheumatic fever in an Aboriginal population. Lancet 356: 1167– 1169.

53. Hendley, J. O.,, M. A. Sande,, P. M. Stewart,, and J. M. Gwaltney, Jr. 1975. Spread of Streptococcus pneumoniae in families. I. Carriage rates and distribution of types. J. Infect. Dis. 132: 55– 61.

54. Homer, K. A.,, L. Denbow,, R. A. Whiley,, and D. Beighton. 1993. Chondroitin sulfate depolymerase and hyaluronidase activities of viridans streptococci determined by a sensitive spectrophotometric assay. J. Clin. Microbiol. 31: 1648– 1651.

55. Hwang, M. N.,, and G. M. Ederer. 1975. Rapid hippurate hydrolysis method for presumptive identification of group B streptococci. J. Clin. Microbiol. 1: 114– 115.

56. Johnson, D. R.,, and E. L. Kaplan. 1993. A review of the correlation of T-agglutination patterns and M-protein typing and opacity factor production in the identification of group A streptococci. J. Med. Microbiol. 38: 311– 315.

57. Jones, N.,, J. F. Bohnsack,, S. Takahashi,, K. A. Oliver,, M. S. Chan,, F. Kunst,, P. Glaser,, C. Rusniok,, D. W. Crook,, R. M. Harding,, N. Bisharat,, and B. G. Spratt. 2003. Multilocus sequence typing system for group B streptococcus. J. Clin. Microbiol. 41: 2530– 2536.

58. Kaplan, E. L.,, C. D. Rothermel,, and D. R. Johnson. 1998. Antistreptolysin O and anti-deoxyribonuclease B titers: normal values for children ages 2 to 12 in the United States. Pediatrics 101: 86– 88.

59. Kawamura, Y.,, X. G. Hou,, F. Sultana,, H. Miura,, and T. Ezaki. 1995. Determination of 16S rRNA sequences of Streptococcus mitis and Streptococcus gordonii and phylogenetic relationships among members of the genus Streptococcus. Int. J. Syst. Bacteriol. 45: 406– 408.

60. Kawamura, Y.,, X. G. Hou,, Y. Todome,, F. Sultana,, K. Hirose,, S. E. Shu,, T. Ezaki,, and H. Ohkuni. 1998. Streptococcus peroris sp. nov. and Streptococcus infantis sp. nov., new members of the Streptococcus mitis group, isolated from human clinical specimens. Int. J. Syst. Bacteriol. 48( Pt.3): 921– 927.

61. Keith, E. R.,, R. G. Podmore,, T. P. Anderson,, and D. R. Murdoch. 2006. Characteristics of Streptococcus pseudopneumoniae isolated from purulent sputum samples. J. Clin. Microbiol. 44: 923– 927.

62. Kellner, J. D.,, O. G. Vanderkooi,, J. MacDonald,, D. L. Church,, G. J. Tyrrell,, and D. W. Scheifele. 2009. Changing epidemiology of invasive pneumococcal disease in Canada, 1998-2007: update from the Calgary-area Streptococcus pneumoniae research (CASPER) study. Clin. Infect. Dis. 49: 205– 212.

63. Kellogg, J. A. 1990. Suitability of throat culture procedures for detection of group A streptococci and as reference standards for evaluation of streptococcal antigen detection kits. J. Clin. Microbiol. 28: 165– 169.

64. Kikuchi, K.,, T. Enari,, K. Totsuka,, and K. Shimizu. 1995. Comparison of phenotypic characteristics, DNADNA hybridization results, and results with a commercial rapid biochemical and enzymatic reaction system for identification of viridans group streptococci. J. Clin. Microbiol. 33: 1215– 1222.

65. Kilian, M.,, L. Mikkelsen,, and J. Henrichsen. 1989. Taxonomic study of viridans streptococci: description of Streptococcus gordonii sp. nov. and emended descriptions of Streptococcus sanguis (White and Niven 1946), Streptococcus oralis (Bridge and Sneath 1982), and Streptococcus mitis (Andrews and Horder 1906). Int. J. Syst. Bacteriol. 39: 471– 484.

66. Kilian, M.,, K. Poulsen,, T. Blomqvist,, L. S. Havarstein,, M. Bek-Thomsen,, H. Tettelin,, and U. B. Sorensen. 2008. Evolution of Streptococcus pneumoniae and its close commensal relatives. PLoS One 3: e2683.

67. Kimura, K.,, S. Suzuki,, J. Wachino,, H. Kurokawa,, K. Yamane,, N. Shibata,, N. Nagano,, H. Kato,, K. Shibayama,, and Y. Arakawa. 2008. First molecular characterization of group B streptococci with reduced penicillin susceptibility. Antimicrob. Agents Chemother. 52: 2890– 2897.

68. Kirby, R.,, and K. L. Ruoff. 1995. Cost-effective, clinically relevant method for rapid identification of beta-hemolytic streptococci and enterococci. J. Clin. Microbiol. 33: 1154– 1157.

69. Klein, R. S.,, R. A. Recco,, M. T. Catalano,, S. C. Edberg,, J. I. Casey,, and N. H. Steigbigel. 1977. Association of Streptococcus bovis with carcinoma of the colon. N. Engl. J. Med. 297: 800– 802.

70. Kohler, W. 2007. The present state of species within the genera Streptococcus and Enterococcus. Int. J. Med. Microbiol. 297: 133– 150.

71. Kong, F.,, S. Gowan,, D. Martin,, G. James,, and G. L. Gilbert. 2002. Serotype identification of group B streptococci by PCR and sequencing. J. Clin. Microbiol. 40: 216– 226.

72. Kurzynski, T. A.,, and C. K. Meise. 1979. Evaluation of sulfamethoxazole-trimethoprim blood agar plates for recovery of group A streptococci from throat cultures. J. Clin. Microbiol. 9: 189– 193.

73. Lefevre, J. C.,, G. Faucon,, A. M. Sicard,, and A. M. Gasc. 1993. DNA fingerprinting of Streptococcus pneumoniae strains by pulsed-field gel electrophoresis. J. Clin. Microbiol. 31: 2724– 2728.

74. Malhotra-Kumar, S.,, S. Wang,, C. Lammens,, S. Chapelle,, and H. Goossens. 2003. Bacitracin-resistant clone of Streptococcus pyogenes isolated from pharyngitis patients in Belgium. J. Clin. Microbiol. 41: 5282– 5284.

75. Mandell, L. A.,, J. G. Bartlett,, S. F. Dowell,, T. M. File, Jr.,, D. M. Musher,, and C. Whitney. 2003. Update of practice guidelines for the management of community-acquired pneumonia in immunocompetent adults. Clin. Infect. Dis. 37: 1405– 1433.

76. Mandell, L. A.,, R. G. Wunderink,, A. Anzueto,, J. G. Bartlett,, G. D. Campbell,, N. C. Dean,, S. F. Dowell,, T. M. File, Jr.,, D. M. Musher,, M. S. Niederman,, A. Torres,, and C. G. Whitney. 2007. Infectious Diseases Society of America/American Thoracic Society consensus guidelines on the management of community-acquired pneumonia in adults. Clin. Infect. Dis. 44( Suppl. 2): S27– S72.

77. Martin, D. R.,, J. M. Stanhope,, and L. A. Finch. 1977. Delayed culture of group-A streptococci: an evaluation of variables in methods of examining throat swabs. J. Med. Microbiol. 10: 249– 253.

78. Mein, J.,, and G. Lum. 1999. CSF bacterial antigen detection tests offer no advantage over Gram’s stain in the diagnosis of bacterial meningitis. Pathology 31: 67– 69.

79. Messmer, T. O.,, J. S. Sampson,, A. Stinson,, B. Wong,, G. M. Carlone,, and R. R. Facklam. 2004. Comparison of four polymerase chain reaction assays for specificity in the identification of Streptococcus pneumoniae. Diagn. Microbiol. Infect. Dis. 49: 249– 254.

80. Mihaila-Amrouche, L.,, A. Bouvet,, and J. Loubinoux. 2004. Clonal spread of emm type 28 isolates of Streptococcus pyogenes that are multiresistant to antibiotics. J. Clin. Microbiol. 42: 3844– 3846.

81. Moisi, J. C.,, S. K. Saha,, A. G. Falade,, B. M. Njanpop- Lafourcade,, J. Oundo,, A. K. Zaidi,, S. Afroj,, R. A. Bakare,, J. K. Buss,, R. Lasi,, J. Mueller,, A. A. Odekanmi,, L. Sangare,, J. A. Scott,, M. D. Knoll,, O. S. Levine,, and B. D. Gessner. 2009. Enhanced diagnosis of pneumococcal meningitis with use of the Binax NOW immunochromatographic test of Streptococcus pneumoniae antigen: a multisite study. Clin. Infect. Dis. 48( Suppl. 2): S49– S56.

82. Mora, M.,, G. Bensi,, S. Capo,, F. Falugi,, C. Zingaretti,, A. G. Manetti,, T. Maggi,, A. R. Taddei,, G. Grandi,, and J. L. Telford. 2005. Group A Streptococcus produce pilus-like structures containing protective antigens and Lancefield T antigens. Proc. Natl. Acad. Sci. USA 102: 15641– 15646.

83. Murdoch, D. R.,, R. T. Laing,, G. D. Mills,, N. C. Karalus,, G. I. Town,, S. Mirrett,, and L. B. Reller. 2001. Evaluation of a rapid immunochromatographic test for detection of Streptococcus pneumoniae antigen in urine samples from adults with community- acquired pneumonia. J. Clin. Microbiol. 39: 3495– 3498.

84. Murdoch, D. R.,, and L. B. Reller. 2001. Antimicrobial susceptibilities of group B streptococci isolated from patients with invasive disease: 10-year perspective. Antimicrob. Agents Chemother. 45: 3623– 3624.

85. Murray, P. R.,, A. D. Wold,, C. A. Schreck,, and J. A. Washington II. 1976. Effects of selective media and atmosphere of incubation on the isolation of group A streptococci. J. Clin. Microbiol. 4: 54– 56.

86. Musser, J. M.,, B. M. Gray,, P. M. Schlievert,, and M. E. Pichichero. 1992. Streptococcus pyogenes pharyngitis: characterization of strains by multilocus enzyme genotype, M and T protein serotype, and pyrogenic exotoxin gene probing. J. Clin. Microbiol. 30: 600– 603.

87. Musser, J. M.,, V. Kapur,, J. Szeto,, X. Pan,, D. S. Swanson,, and D. R. Martin. 1995. Genetic diversity and relationships among Streptococcus pyogenes strains expressing serotype M1 protein: recent intercontinental spread of a subclone causing episodes of invasive disease. Infect. Immun. 63: 994– 1003.

88. Pergola, V.,, G. Di Salvo,, G. Habib,, J. F. Avierinos,, E. Philip,, J. M. Vailloud,, F. Thuny,, J. P. Casalta,, P. Ambrosi,, M. Lambert,, A. Riberi,, A. Ferracci,, T. Mesana,, D. Metras,, J. R. Harle,, P. J. Weiller,, D. Raoult,, and R. Luccioni. 2001. Comparison of clinical and echocardiographic characteristics of Streptococcus bovis endocarditis with that caused by other pathogens. Am. J. Cardiol. 88: 871– 875.

89. Peterson, L. R.,, and R. B. Thomson, Jr. 1999. Use of the clinical microbiology laboratory for the diagnosis and management of infectious diseases related to the oral cavity. Infect. Dis. Clin. N. Am. 13: 775– 795.

90. Pires, R.,, D. Rolo,, R. Mato,, J. Feio de Almeida,, C. Johansson,, B. Henriques-Normark,, A. Morais,, A. Brito- Avo,, J. Goncalo-Marques,, and I. Santos-Sanches. 2009. Resistance to bacitracin in Streptococcus pyogenes from oropharyngeal colonization and noninvasive infections in Portugal was caused by two clones of distinct virulence genotypes. FEMS Microbiol. Lett. 296: 235– 240.

91. Podbielski, A.,, O. Blankenstein,, and R. Lutticken. 1994. Molecular characterization of the cfb gene encoding group B streptococcal CAMP-factor. Med. Microbiol. Immunol. ( Berlin) 183: 239– 256.

92. Pokorski, S. J.,, E. A. Vetter,, P. C. Wollan,, and F. R. Cockerill III. 1994. Comparison of Gen-Probe Group A streptococcus Direct Test with culture for diagnosing streptococcal pharyngitis. J. Clin. Microbiol. 32: 1440– 1443.

93. Poyart, C.,, C. Pierre,, G. Quesne,, B. Pron,, P. Berche,, and P. Trieu-Cuot. 1997. Emergence of vancomycin resistance in the genus Streptococcus: characterization of a vanB transferable determinant in Streptococcus bovis. Antimicrob. Agents Chemother. 41: 24– 29.

94. Poyart, C.,, G. Quesne,, S. Coulon,, P. Berche,, and P. Trieu-Cuot. 1998. Identification of streptococci to species level by sequencing the gene encoding the manganese-dependent superoxide dismutase. J. Clin. Microbiol. 36: 41– 47.

95. Poyart, C.,, A. Tazi,, H. Reglier-Poupet,, A. Billoet,, N. Tavares,, J. Raymond,, and P. Trieu-Cuot. 2007. Multiplex PCR assay for rapid and accurate capsular typing of group B streptococci. J. Clin. Microbiol. 45: 1985– 1988.

96. Ratner, H. B.,, L. S. Weeks,, and C. W. Stratton. 1986. Evaluation of spot CAMP test for identification of group B streptococci. J. Clin. Microbiol. 24: 296– 297.

97. Regev-Yochay, G.,, M. Raz,, R. Dagan,, N. Porat,, B. Shainberg,, E. Pinco,, N. Keller,, and E. Rubinstein. 2004. Nasopharyngeal carriage of Streptococcus pneumoniae by adults and children in community and family settings. Clin. Infect. Dis. 38: 632– 639.

98. Richter, S. S.,, K. P. Heilmann,, C. L. Dohrn,, F. Riahi,, S. E. Beekmann,, and G. V. Doern. 2008. Accuracy of phenotypic methods for identification of Streptococcus pneumoniae isolates included in surveillance programs. J. Clin. Microbiol. 46: 2184– 2188.

99. Richter, S. S.,, K. P. Heilmann,, C. L. Dohrn,, F. Riahi,, S. E. Beekmann,, and G. V. Doern. 2009. Changing epidemiology of antimicrobial-resistant Streptococcus pneumoniae in the United States, 2004-2005. Clin. Infect. Dis. 48: e23– e33.

100. Rosa-Fraile, M.,, J. Rodriguez-Granger,, M. Cueto-Lopez,, A. Sampedro,, E. B. Gaye,, J. M. Haro,, and A. Andreu. 1999. Use of Granada medium to detect group B streptococcal colonization in pregnant women. J. Clin. Microbiol. 37: 2674– 2677.

101. Ruoff, K. L.,, S. I. Miller,, C. V. Garner,, M. J. Ferraro,, and S. B. Calderwood. 1989. Bacteremia with Streptococcus bovis and Streptococcus salivarius: clinical correlates of more accurate identification of isolates. J. Clin. Microbiol. 27: 305– 308.

102. Rurangirwa, F. R.,, C. A. Teitzel,, J. Cui,, D. M. French,, P. L. McDonough,, and T. Besser. 2000. Streptococcus didelphis sp. nov., a streptococcus with marked catalase activity isolated from opossums (Didelphis virginiana) with suppurative dermatitis and liver fibrosis. Int. J. Syst. Evol. Microbiol. 50( Pt. 2): 759– 765.

103. Schlegel, L.,, F. Grimont,, E. Ageron,, P. A. Grimont,, and A. Bouvet. 2003. Reappraisal of the taxonomy of the Streptococcus bovis/Streptococcus equinus complex and related species: description of Streptococcus gallolyticus subsp. gallolyticus subsp. nov., S. gallolyticus subsp. macedonicus subsp. nov. and S. gallolyticus subsp. pasteurianus subsp. nov. Int. J. Syst. Evol. Microbiol. 53: 631– 645.

104. Schlegel, L.,, F. Grimont,, M. D. Collins,, B. Regnault,, P. A. Grimont,, and A. Bouvet. 2000. Streptococcus infantarius sp. nov., Streptococcus infantarius subsp. infantarius subsp. nov. and Streptococcus infantarius subsp. coli subsp. nov., isolated from humans and food. Int. J. Syst. Evol. Microbiol. 50( Pt. 4): 1425– 1434.

105. Schrag, S.,, R. Gorwitz,, K. Fultz-Butts,, and A. Schuchat. 2002. Prevention of perinatal group B streptococcal disease. Revised guidelines from CDC. MMWR Recommend. Rep. 51: 1– 22.

106. Schrag, S. J.,, S. Zywicki,, M. M. Farley,, A. L. Reingold,, L. H. Harrison,, L. B. Lefkowitz,, J. L. Hadler,, R. Danila,, P. R. Cieslak,, and A. Schuchat. 2000. Group B streptococcal disease in the era of intrapartum antibiotic prophylaxis. N. Engl. J. Med. 342: 15– 20.

107. Schuchat, A.,, T. Hilger,, E. Zell,, M. M. Farley,, A. Reingold,, L. Harrison,, L. Lefkowitz,, R. Danila,, K. Stefonek,, N. Barrett,, D. Morse,, and R. Pinner. 2001. Active bacterial core surveillance of the emerging infections program network. Emerg. Infect. Dis. 7: 92– 99.

108. Shet, A.,, and E. L. Kaplan. 2002. Clinical use and interpretation of group A streptococcal antibody tests: a practical approach for the pediatrician or primary care physician. Pediatr. Infect. Dis. J. 21:42- 46, 427– 430.

109. Skoff, T. H.,, M. M. Farley,, S. Petit,, A. S. Craig,, W. Schaffner,, K. Gershman,, L. H. Harrison,, R. Lynfield,, J. Mohle-Boetani,, S. Zansky,, B. A. Albanese,, K. Stefonek,, E. R. Zell,, D. Jackson,, T. Thompson,, and S. J. Schrag. 2009. Increasing burden of invasive group B streptococcal disease in nonpregnant adults, 1990-2007. Clin. Infect. Dis. 49: 85– 92.

110. Slotved, H. C.,, M. Kaltoft,, I. C. Skovsted,, M. B. Kerrn,, and F. Espersen. 2004. Simple, rapid latex agglutination test for serotyping of pneumococci (Pneumotest-Latex). J. Clin. Microbiol. 42: 2518– 2522.

111. Smith, D. J. 2002. Dental caries vaccines: prospects and concerns. Crit. Rev. Oral Biol. Med. 13: 335– 349.

112. Stevens, D. L. 2001. Invasive streptococcal infections. J. Infect. Chemother. 7: 69– 80.

113. Summanen, P. H.,, M. C. Rowlinson,, J. Wooton,, and S. M. Finegold. 2009. Evaluation of genotypic and phenotypic methods for differentiation of the members of the Anginosus group streptococci. Eur. J. Clin. Microbiol. Infect. Dis. 28: 1123– 1128.

114. Swedo, S. E.,, H. L. Leonard,, B. B. Mittleman,, A. J. Allen,, J. L. Rapoport,, S. P. Dow,, M. E. Kanter,, F. Chapman,, and J. Zabriskie. 1997. Identification of children with pediatric autoimmune neuropsychiatric disorders associated with streptococcal infections by a marker associated with rheumatic fever. Am. J. Psychiatry 154: 110– 112.

115. Thinkhamrop, J.,, S. Limpongsanurak,, M. R. Festin,, S. Daly,, A. Schuchat,, P. Lumbiganon,, E. Zell,, T. Chipato,, A. A. Win,, M. J. Perilla,, J. E. Tolosa,, and C. G. Whitney. 2003. Infections in international pregnancy study: performance of the optical immunoassay test for detection of group B streptococcus. J. Clin. Microbiol. 41: 5288– 5290.

116. Thomas, J. G. 1994. Routine CSF antigen detection for agents associated with bacterial meningitis: another point of view. Clin. Microbiol. Newsl. 16: 89– 95.

117. Thornsberry, C.,, D. F. Sahm,, L. J. Kelly,, I. A. Critchley,, M. E. Jones,, A. T. Evangelista,, and J. A. Karlowsky. 2002. Regional trends in antimicrobial resistance among clinical isolates of Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis in the United States: results from the TRUST Surveillance Program, 1999-2000. Clin. Infect. Dis. 34( Suppl. 1): S4– S16.

118. Tong, H.,, X. Gao,, and X. Dong. 2003. Streptococcus oligofermentans sp. nov., a novel oral isolate from caries-free humans. Int. J. Syst. Evol. Microbiol. 53: 1101– 1104.

119. Uhl, J. R.,, S. C. Adamson,, E. A. Vetter,, C. D. Schleck,, W. S. Harmsen,, L. K. Iverson,, P. J. Santrach,, N. K. Henry,, and F. R. Cockerill. 2003. Comparison of Light- Cycler PCR, rapid antigen immunoassay, and culture for detection of group A streptococci from throat swabs. J. Clin. Microbiol. 41: 242– 249.

120. Vandamme, P.,, B. Pot,, E. Falsen,, K. Kersters,, and L. A. Devriese. 1996. Taxonomic study of Lancefield streptococcal groups C, G, and L (Streptococcus dysgalactiae) and proposal of S. dysgalactiae subsp. equisimilis subsp. nov. Int. J. Syst. Bacteriol. 46: 774– 781.

121. van’t Wout, J. W.,, and H. A. Bijlmer. 2005. Bacteremia due to Streptococcus gallolyticus, or the perils of revised nomenclature in bacteriology. Clin. Infect. Dis. 40: 1070– 1071.

122. Vieira, V.,, L. Teixeira,, V. Zahner,, H. Momen,, R. Facklam,, A. Steigerwalt,, D. Brenner,, and A. Castro. 1998. Genetic relationships among the different phenotypes of Streptococcus dysgalactiae strains. Int. J. Syst. Bacteriol. 48: 1231– 1243.

123. Votava, M.,, M. Tejkalova,, M. Drabkova,, V. Unzeitig,, and I. Braveny. 2001. Use of GBS media for rapid detection of group B streptococci in vaginal and rectal swabs from women in labor. Eur. J. Clin. Microbiol. Infect. Dis. 20: 120– 122.

124. Weinstein, M. P.,, K. P. Klugman,, and R. N. Jones. 2009. Rationale for revised penicillin susceptibility breakpoints versus Streptococcus pneumoniae: coping with antimicrobial susceptibility in an era of resistance. Clin. Infect. Dis. 48: 1596– 1600.

125. Weinstein, M. R.,, M. Litt,, D. A. Kertesz,, P. Wyper,, D. Rose,, M. Coulter,, A. McGeer,, R. Facklam,, C. Ostach,, B. M. Willey,, A. Borczyk,, D. E. Low, et al. 1997. Invasive infections due to a fish pathogen, Streptococcus iniae. N. Engl. J. Med. 337: 589– 594.

126. Welch, D. F.,, D. Hensel,, D. Pickett,, and S. Johnson. 1991. Comparative evaluation of selective and nonselective culture techniques for isolation of group A beta-hemolytic streptococci. Am. J. Clin. Pathol. 95: 587– 590.

127. West, P. W.,, H. A. Foster,, Q. Electricwala,, and A. Alex. 1996. Comparison of five methods for the determination of arginine hydrolysis by viridans streptococci. J. Med. Microbiol. 45: 501– 504.

128. Whiley, R. A.,, and D. Beighton. 1991. Emended descriptions and recognition of Streptococcus constellatus, Streptococcus intermedius, and Streptococcus anginosus as distinct species. Int. J. Syst. Bacteriol. 41: 1– 5.

129. Whiley, R. A.,, H. Fraser,, J. M. Hardie,, and D. Beighton. 1990. Phenotypic differentiation of Streptococcus intermedius, Streptococcus constellatus, and Streptococcus anginosus strains within the “Streptococcus milleri group.” J. Clin. Microbiol. 28: 1497– 1501.

130. Whiley, R. A.,, L. M. Hall,, J. M. Hardie,, and D. Beighton. 1999. A study of small-colony, beta-haemolytic, Lancefield group C streptococci within the anginosus group: description of Streptococcus constellatus subsp. pharyngis subsp. nov., associated with the human throat and pharyngitis. Int. J. Syst. Bacteriol. 49: 1443– 1449.

131. Willcox, M. D.,, H. Zhu,, and K. W. Knox. 2001. Streptococcus australis sp. nov., a novel oral streptococcus. Int. J. Syst. Evol. Microbiol. 51: 1277– 1281.

Tables

Streptococcus

/content/10.1128/9781555816728.chap20.tab20-1

TABLE 1

Phenotypic characteristics of beta-hemolytic streptococci a

a Symbols and abbreviations: +, positive; −, negative; v, variable.

b S. dysgalactiae subsp. dysgalactiae is alpha-hemolytic on sheep blood agar plates.

c S. equi subsp. zooepidemicus, S. canis, and S. porcinus are primarily animal pathogens that are only rarely isolated from humans.

d Species included in the S. anginosus group can be beta-hemolytic, alpha-hemolytic, or nonhemolytic on sheep blood agar plates.

e Large colony size refers to colonies >0.5 mm after 24 h of incubation, whereas small colony size is <0.5 mm.

f Presence of the enzyme pyrrolidonyl aminopeptidase.

g CAMP factor reaction (cohemolysis in the presence of the Staphylococcus aureus beta-hemolysin)

h Voges-Proskauer test (formation of acetoin from glucose fermentation).

i Parenthetical entries indicate hosts from which the organism is rarely isolated.

10.1128/9781555816728/tab20-1_thmb.gif

10.1128/9781555816728/tab20-1.gif

TABLE 1

Phenotypic characteristics of beta-hemolytic streptococci a

a Symbols and abbreviations: +, positive; −, negative; v, variable.

b S. dysgalactiae subsp. dysgalactiae is alpha-hemolytic on sheep blood agar plates.

c S. equi subsp. zooepidemicus, S. canis, and S. porcinus are primarily animal pathogens that are only rarely isolated from humans.

d Species included in the S. anginosus group can be beta-hemolytic, alpha-hemolytic, or nonhemolytic on sheep blood agar plates.

e Large colony size refers to colonies >0.5 mm after 24 h of incubation, whereas small colony size is <0.5 mm.

f Presence of the enzyme pyrrolidonyl aminopeptidase.

g CAMP factor reaction (cohemolysis in the presence of the Staphylococcus aureus beta-hemolysin)

h Voges-Proskauer test (formation of acetoin from glucose fermentation).

i Parenthetical entries indicate hosts from which the organism is rarely isolated.

/content/10.1128/9781555816728.chap20.tab20-2

TABLE 2

Phenotypic characteristics of major streptococcal groups a

a Symbols and abbreviations: +, positive; −, negative; v, variable.

b This group comprises the species S. mitis, S. sanguinis, S. parasanguinis, S. gordonii, S. cristatus, S. oralis, S. infantis, S. peroris, S. australis, S. sinensis, S. orisratti, S. oligofermentans, S. massiliensis. S. sanguinis, S. parasanguinis, S. gordonii, and S. cristatus are arginine hydrolysis positive; other species from the S. mitis group are arginine hydrolysis negative.

c S. anginosus, S. constellatus, and S. intermedius belong to the S. anginosus group.

d The S. mutans group includes S. mutans, S. sobrinus, and the following species rarely isolated from humans: S. criceti, S. ratti, and S. downei.

e The S. salivarius group contains S. salivarius, S. vestibularis, and S. thermophilus. S. salivarius is variable, S. vestibularis is positive, and S. thermophilus is negative for urea hydrolysis.

f The S. bovis group now includes S. equinus, S. gallolyticus, S. infantarius, and S. alactolyticus. S. gallolyticus subsp. gallolyticus is positive for the acidification of mannitol; the other species from the S. bovis group are negative.

10.1128/9781555816728/tab20-2_thmb.gif

10.1128/9781555816728/tab20-2.gif

TABLE 2

Phenotypic characteristics of major streptococcal groups a

a Symbols and abbreviations: +, positive; −, negative; v, variable.

c S. anginosus, S. constellatus, and S. intermedius belong to the S. anginosus group.

d The S. mutans group includes S. mutans, S. sobrinus, and the following species rarely isolated from humans: S. criceti, S. ratti, and S. downei.

e The S. salivarius group contains S. salivarius, S. vestibularis, and S. thermophilus. S. salivarius is variable, S. vestibularis is positive, and S. thermophilus is negative for urea hydrolysis.

/content/10.1128/9781555816728.chap20.tab20-3

TABLE 3

Phenotypic characteristics of streptococcal species of the S. anginosus group a

a Symbols and abbreviations: +, positive; –, negative; v, variable. Data are from Summanen et al. ( 113 ), Facklam ( 36 ), and Whiley et al. ( 129 ).

b The species S. constellatus subsp. pharyngis is β-D-fucosidase, β-D-acetylglucosaminidase, β-D-acetylgalactosaminidase, and β-D-glucosidase positive, in contrast to S. constellatus subsp. constellatus, which is negative for these activities.

c Voges-Proskauer test (formation of acetoin from glucose fermentation).

10.1128/9781555816728/tab20-3_thmb.gif

10.1128/9781555816728/tab20-3.gif

TABLE 3

Phenotypic characteristics of streptococcal species of the S. anginosus group a

a Symbols and abbreviations: +, positive; –, negative; v, variable. Data are from Summanen et al. ( 113 ), Facklam ( 36 ), and Whiley et al. ( 129 ).

c Voges-Proskauer test (formation of acetoin from glucose fermentation).