Leptospira

Paul N. Levett

doi:10.1128/9781555816728.ch55

Chapter 55 : Leptospira

Author: Paul N. Levett

Content Type: Reference

Publication Year: 2011

Category: Clinical Microbiology

Book DOI: 10.1128/9781555816728

Chapter DOI: 10.1128/9781555816728.ch55

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Abstract:

The genus Leptospira is comprised of spiral-shaped bacteria with hooked ends. This genus, along with the genera Leptonema and Turneriella, makes up the family Leptospiraceae within the order Spirochaetales and class Spirochaetes of the proposed phylum Spirochaetes. The genus was formerly divided into two species: Leptospira interrogans, comprising all pathogenic strains, and L. biflexa, containing the saprophytic strains isolated from the environment. The molecularly based taxonomy of Leptospira necessitates the identification of isolates to both the species and serovar levels. Species identification is most practically done by sequence analysis, using rrs (16s rRNA), gyrB, rpoB, or secY. The definitive serologic investigation in leptospirosis remains the microscopic agglutination test (MAT), in which patients sera are reacted with live or killed antigen suspensions of leptospiral serovars. Leptospires are susceptible to many antimicrobial agents, including β-lactams, macrolides, tetracyclines, fluoroquinolones, and streptomycin. Despite recent advances in molecular detection and characterization of leptospires and in the development of rapid serologic tests, there are still relatively few laboratories throughout the world with the appropriate capabilities for Leptospira diagnostics.

Citation: Levett P. 2011. Leptospira, p 916-923. In Versalovic J, Carroll K, Funke G, Jorgensen J, Landry M, Warnock D (ed), Manual of Clinical Microbiology, 10th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816728.ch55

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Leptospira

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FIGURE 1

Phylogenetic relationships based on rrs (16S rRNA gene) sequences between the genera of the Leptospiraceae. The scale bar represents a 2% difference in sequence.

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FIGURE 1

Phylogenetic relationships based on rrs (16S rRNA gene) sequences between the genera of the Leptospiraceae. The scale bar represents a 2% difference in sequence.

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FIGURE 2

Scanning electron micrograph of leptospiral cells bound to a 0.2-µm-pore-size filter. Magnification, approximately ×3,500. Source: Public Health Image Library.

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FIGURE 2

Scanning electron micrograph of leptospiral cells bound to a 0.2-µm-pore-size filter. Magnification, approximately ×3,500. Source: Public Health Image Library.

References

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1. Adler, B.,, and S. Faine. 1978. The antibodies involved in the human immune response to leptospiral infection. J. Med. Microbiol. 11: 387– 400.

2. Ahmed, N.,, S. M. Devi,, L. Valverde Mde,, P. Vijayachari,, R. S. Machang’u,, W. A. Ellis,, and R. A. Hartskeerl. 2006. Multilocus sequence typing method for identification and genotypic classification of pathogenic Leptospira species. Ann. Clin. Microbiol. Antimicrob. 5: 28.

3. Alexander, A. D., 1986. Serological diagnosis of leptospirosis, p. 435– 439. In N. R. Rose,, H. Friedman,, and J. L. Fahey (ed.), Manual of Clinical Laboratory Immunology, 3rd ed. American Society for Microbiology, Washington, DC.

4. Bajani, M. D.,, D. A. Ashford,, S. L. Bragg,, C. W. Woods,, T. Aye,, R. A. Spiegel,, B. D. Plikaytis,, B. A. Perkins,, M. Phelan,, P. N. Levett,, and R. S. Weyant. 2003. Evaluation of four commercially available rapid serologic tests for diagnosis of leptospirosis. J. Clin. Microbiol. 41: 803– 809.

5. Brandão, A. P.,, E. D. Camargo,, E. D. da Silva,, M. V. Silva,, and R. V. Abrão. 1998. Macroscopic agglutination test for rapid diagnosis of human leptospirosis. J. Clin. Microbiol. 36: 3138– 3142.

6. Brenner, D. J.,, A. F. Kaufmann,, K. R. Sulzer,, A. G. Steigerwalt,, F. C. Rogers,, and R. S. Weyant. 1999. Further determination of DNA relatedness between serogroups and serovars in the family Leptospiraceae with a proposal for Leptospira alexanderi sp. nov. and four new Leptospira genomospecies. Int. J. Syst. Bacteriol. 49: 839– 858.

7. Brown, C. A.,, A. W. Roberts,, M. A. Miller,, D. A. David,, S. A. Brown,, C. A. Bolin,, J. Jarecki-Black,, C. E. Greene,, and D. Miller Liebl. 1996. Leptospira interrogans serovar grippotyphosa infection in dogs. J. Am. Vet. Med. Assoc. 209: 1265– 1267.

8. Brown, P. D.,, D. G. Carrington,, C. Gravekamp,, H. van de Kemp,, C. N. Edwards,, S. R. Jones,, P. R. Prussia,, S. Garriques,, W. J. Terpstra,, and P. N. Levett. 2003. Direct detection of leptospiral material in human postmortem samples. Res. Microbiol. 154: 581– 586.

9. Brown, P. D.,, C. Gravekamp,, D. G. Carrington,, H. Van de Kemp,, R. A. Hartskeerl,, C. N. Edwards,, C. O. R. Everard,, W. J. Terpstra,, and P. N. Levett. 1995. Evaluation of the polymerase chain reaction for early diagnosis of leptospirosis. J. Med. Microbiol. 43: 110– 114.

10. Centers for Disease Control and Prevention. 1997. Case definitions for infectious conditions under public health surveillance. MMWR Morb. Mortal. Wkly. Rep. 46( RR-10): 49.

11. Cerqueira, G. M.,, and M. Picardeau. 2009. A century of Leptospira strain typing. Infect. Genet. Evol. 9: 760– 768.

12. Chappel, R. J.,, M. G. Goris,, M. F. Palmer,, and R. A. Hartskeerl. 2004. Impact of proficiency testing on results of the microscopic agglutination test for diagnosis of leptospirosis. J. Clin. Microbiol. 42: 5484– 5488.

13. Cumberland, P.,, C. O. R. Everard,, J. G. Wheeler,, and P. N. Levett. 2001. Persistence of anti-leptospiral IgM, IgG and agglutinating antibodies in patients presenting with acute febrile illness in Barbados 1979-1989. Eur. J. Epidemiol. 17: 601– 608.

14. Cumberland, P. C.,, C. O. R. Everard,, and P. N. Levett. 1999. Assessment of the efficacy of the IgM enzyme-linked immunosorbent assay (ELISA) and microscopic agglutination test (MAT) in the diagnosis of acute leptospirosis. Am. J. Trop. Med. Hyg. 61: 731– 734.

15. Dikken, H.,, and E. Kmety. 1978. Serological typing methods of leptospires. Methods Microbiol. 11: 259– 307.

16. Edwards, C. N.,, and P. N. Levett. 2004. Prevention and treatment of leptospirosis. Expert Rev. Anti-Infect. Ther. 2: 293– 298.

17. Ellinghausen, H. C. 1983. Growth, cultural characteristics, and antibacterial sensitivity of Leptospira interrogans serovar hardjo. Cornell Vet. 73: 225– 239.

18. Ellinghausen, H. C.,, and W. G. McCullough. 1965. Nutrition of Leptospira pomona and growth of 13 other serotypes: fractionation of oleic albumin complex and a medium of bovine albumin and polysorbate 80. Am. J. Vet. Res. 26: 45– 51.

19. Faine, S.,, B. Adler,, C. Bolin,, and P. Perolat. 1999. Leptospira and Leptospirosis, 2nd ed. MedSci, Melbourne, Australia.

20. Fearnley, C.,, P. R. Wakeley,, J. Gallego-Beltran,, C. Dalley,, S. Williamson,, C. Gaudie,, and M. J. Woodward. 2008. The development of a real-time PCR to detect pathogenic Leptospira species in kidney tissue. Res. Vet. Sci. 85: 8– 16.

21. Feresu, S. B.,, C. A. Bolin,, H. van de Kemp,, and H. Korver. 1999. Identification of a serogroup Bataviae Leptospira strain isolated from an ox in Zimbabwe. Zentralbl. Bakteriol. 289: 19– 29.

22. Galloway, R. L.,, and P. N. Levett. 2010. Application and validation of PFGE for serovas identification of Leptospira clinical isolates. PLoS Neglected Trop. Dis. 4: e824.

23. Ganoza, C. A.,, M. A. Matthias,, D. Collins-Richards,, K. C. Brouwer,, C. B. Cunningham,, E. R. Segura,, R. H. Gilman,, E. Gotuzzo,, and J. M. Vinetz. 2006. Determining risk for severe leptospirosis by molecular analysis of environmental surface waters for pathogenic Leptospira. PLoS Med. 3: 1329– 1340.

24. Garrity, G. M.,, and J. G. Holt,. 2001. The road map to the Manual, p. 119– 166. In D. R. Boone,, R. W. Castenholtz,, and G. M. Garrity(ed.), Bergey’s Manual of Systematic Bacteriology, 2nd ed., vol. 1. Springer-Verlag, New York, NY.

25. Gelman, S. S.,, A. V. Gundlapalli,, D. Hale,, A. Croft,, M. Hindiyeh,, and K. C. Carroll. 2002. Spotting the spirochete: rapid diagnosis of leptospirosis in two returned travelers. J. Travel Med. 9: 165– 167.

26. Goldstein, S. F.,, and N. W. Charon. 1988. Motility of the spirochete Leptospira. Cell Motil. Cytoskeleton 9: 101– 110.

27. Gussenhoven, G. C.,, M. A. W. G. van der Hoorn,, M. G. A. Goris,, W. J. Terpstra,, R. A. Hartskeerl,, B. W. Mol,, C. W. van Ingen,, and H. L. Smits. 1997. LEPTO dipstick, a dipstick assay for detection of Leptospira-specific immunoglobulin M antibodies in human sera. J. Clin. Microbiol. 35: 92– 97.

28. Herrmann, J. L.,, E. Bellenger,, P. Perolat,, G. Baranton,, and I. Saint Girons. 1992. Pulsed-field gel electrophoresis of NotI digests of leptospiral DNA: a new rapid method of serovar identification. J. Clin. Microbiol. 30: 1696– 1702.

29. Johnson, R. C.,, and S. Faine,. 1984. Leptospira, p. 62– 67. In N. R. Krieg, and J. G. Holt (ed.), Bergey’s Manual of Systematic Bacteriology, vol. 1. Williams & Wilkins, Baltimore, MD.

30. Johnson, R. C.,, and V. G. Harris. 1967. Differentiation of pathogenic and saprophytic leptospires. 1. Growth at low temperatures. J. Bacteriol. 94: 27– 31.

31. Kmety, E.,, and H. Dikken. 1993. Classification of the species Leptospira interrogans and history of its serovars. University Press Groningen, Groningen, The Netherlands.

32. Ko, A. I.,, C. Goarant,, and M. Picardeau. 2009. Leptospira: the dawn of the molecular genetics era for an emerging zoonotic pathogen. Nat. Rev. Microbiol. 7: 736– 747.

33. La Scola, B.,, L. T. M. Bui,, G. Baranton,, A. Khamis,, and D. Raoult. 2006. Partial rpoB gene sequencing for identification of Leptospira species. FEMS Microbiol. Lett. 263: 142– 147.

34. Lau, C.,, L. Smythe,, and P. Weinstein. 2010. Leptospirosis: an emerging disease in travellers. Travel Med. Infect. Dis. 8: 33– 39.

35. Levett, P. N., 2007. Leptospira, p. 963– 970. In P. R. Murray,, E. J. Baron,, J. H. Jorgensen,, M. L. Landry,, and M. A. Pfaller (ed.), Manual of Clinical Microbiology, 9th ed. ASM Press, Washington, DC.

36. Levett, P. N. 2001. Leptospirosis. Clin. Microbiol. Rev. 14: 296– 326.

37. Levett, P. N. 2007. Sequence-based typing of Leptospira: epidemiology in the genomic era. PLoS Neglected Trop. Dis. 1: e120.

38. Levett, P. N. 2003. Usefulness of serologic analysis as a predictor of the infecting serovar in patients with severe leptospirosis. Clin. Infect. Dis. 36: 447– 452.

39. Levett, P. N.,, S. L. Branch,, C. U. Whittington,, C. N. Edwards,, and H. Paxton. 2001. Two methods for rapid serological diagnosis of acute leptospirosis. Clin. Diagn. Lab. Immunol. 8: 349– 351.

40. Levett, P. N.,, R. E. Morey,, R. Galloway,, A. G. Steigerwalt,, and W. A. Ellis. 2005. Reclassification of Leptospira parva Hovind-Hougen et al. 1982 as Turneriella parva gen. nov., comb. nov. Int. J. Syst. Evol. Microbiol. 55: 1497– 1499.

41. Levett, P. N.,, R. E. Morey,, R. L. Galloway,, D. E. Turner,, A. G. Steigerwalt,, and L. W. Mayer. 2005. Detection of pathogenic leptospires by real-time quantitative PCR. J. Med. Microbiol. 54: 45– 49.

42. Lourdault, K.,, F. Aviat,, and M. Picardeau. 2009. Use of quantitative real-time PCR for studying the dissemination of Leptospira interrogans in the guinea pig infection model of leptospirosis. J. Med. Microbiol. 58: 648– 655.

43. Matthias, M. A.,, J. N. Ricaldi,, M. Cespedes,, M. M. Diaz,, R. L. Galloway,, M. Saito,, A. G. Steigerwalt,, K. P. Patra,, C. V. Ore,, E. Gotuzzo,, R. H. Gilman,, P. N. Levett,, and J. M. Vinetz. 2008. Human leptospirosis caused by a new, antigenically unique Leptospira associated with a Rattus species reservoir in the Peruvian Amazon. PLoS Neglected Trop. Dis. 2: e213.

44. McCrumb, F. R.,, J. L. Stockard,, C. R. Robinson,, L. H. Turner,, D. G. Levis,, C. W. Maisey,, M. F. Kelleher,, C. A. Gleiser,, and J. E. Smadel. 1957. Leptospirosis in Malaya. I. Sporadic cases among military and civilian personnel. Am. J. Trop. Med. Hyg. 6: 238– 256.

45. Merien, F.,, G. Baranton,, and P. Pérolat. 1995. Comparison of polymerase chain reaction with microagglutination test and culture for diagnosis of leptospirosis. J. Infect. Dis. 172: 281– 285.

46. Merien, F.,, D. Portnoi,, P. Bourhy,, F. Charavay,, A. Berlioz-Arthaud,, and G. Baranton. 2005. A rapid and quantitative method for the detection of Leptospira species in human leptospirosis. FEMS Microbiol. Lett. 249: 139– 147.

47. Monahan, A. M.,, I. S. Miller,, and J. E. Nally. 2009. Leptospirosis: risks during recreational activities. J. Appl. Microbiol. 107: 707– 716.

48. Morey, R. E.,, R. L. Galloway,, S. L. Bragg,, A. G. Steigerwalt,, L. W. Mayer,, and P. N. Levett. 2006. Species-specific identification of Leptospiraceae by 16S rRNA gene sequencing. J. Clin. Microbiol. 44: 3510– 3516.

49. Murray, C. K.,, and D. R. Hospenthal. 2004. Broth microdilution susceptibility testing for Leptospira spp. Antimicrob. Agents Chemother. 48: 1548– 1552.

50. Oie, S.,, K. Hironaga,, A. Koshiro,, H. Konishi,, and Z. Yoshii. 1983. In vitro susceptibilities of five Leptospira strains to 16 antimicrobial agents. Antimicrob. Agents Chemother. 24: 905– 908.

51. Palaniappan, R. U.,, Y. F. Chang,, C. F. Chang,, M. J. Pan,, C. W. Yang,, P. Harpending,, S. P. McDonough,, E. Dubovi,, T. Divers,, J. Qu,, and B. Roe. 2005. Evaluation of lig-based conventional and real time PCR for the detection of pathogenic leptospires. Mol. Cell. Probes 19: 111– 117.

52. Palmer, M. F.,, and W. J. Zochowski. 2000. Survival of leptospires in commercial blood culture systems revisited. J. Clin. Pathol. 53: 713– 714.

53. Panaphut, T.,, S. Domrongkitchaiporn,, A. Vibhagool,, B. Thinkamrop,, and W. Susaengrat. 2003. Ceftriaxone compared with sodium penicillin G for treatment of severe leptospirosis. Clin. Infect. Dis. 36: 1507– 1513.

54. Ressner, R. A.,, M. E. Griffith,, M. L. Beckius,, G. Pimentel,, R. S. Miller,, K. Mende,, S. L. Fraser,, R. L. Galloway,, D. R. Hospenthal,, and C. K. Murray. 2008. Antimicrobial susceptibilities of geographically diverse clinical human isolates of Leptospira. Antimicrob. Agents Chemother. 52: 2750– 2754.

55. Ribeiro, M. A.,, C. S. N. Assis,, and E. C. Romero. 1994. Serodiagnosis of human leptospirosis employing immunodominant antigen. Serodiagn. Immunother. Infect. Dis. 6: 140– 144.

56. Romero, E. C.,, C. R. Caly,, and P. H. Yasuda. 1998. The persistence of leptospiral agglutinins titers in human sera diagnosed by the microscopic agglutination test. Rev. Inst. Med. Trop. São Paulo 40: 183– 184.

57. Segura, E. R.,, C. A. Ganoza,, K. Campos,, J. N. Ricaldi,, S. Torres,, H. Silva,, M. J. Cespedes,, M. A. Matthias,, M. A. Swancutt,, R. Lopez Linan,, E. Gotuzzo,, H. Guerra,, R. H. Gilman,, and J. M. Vinetz. 2005. Clinical spectrum of pulmonary involvement in leptospirosis in a region of endemicity, with quantification of leptospiral burden. Clin. Infect. Dis. 40: 343– 351.

58. Slack, A.,, M. Symonds,, M. Dohnt,, C. Harris,, D. Brookes,, and L. Smythe. 2007. Evaluation of a modified Taqman assay detecting pathogenic Leptospira spp. against culture and Leptospira-specific IgM enzyme-linked immunosorbent assay in a clinical environment. Diagn. Microbiol. Infect. Dis. 57: 361– 366.

59. Slack, A. T.,, T. Kalambaheti,, M. L. Symonds,, M. F. Dohnt,, R. L. Galloway,, A. G. Steigerwalt,, W. Chaicumpa,, G. Bunyaraksyotin,, S. Craig,, B. J. Harrower,, and L. D. Smythe. 2008. Leptospira wolffii sp. nov., isolated from a human with suspected leptospirosis in Thailand. Int. J. Syst. Evol. Microbiol. 58: 2305– 2308.

60. Slack, A. T.,, S. Khairani-Bejo,, M. L. Symonds,, M. F. Dohnt,, R. L. Galloway,, A. G. Steigerwalt,, A. R. Bahaman,, S. Craig,, B. J. Harrower,, and L. D. Smythe. 2009. Leptospira kmetyi sp. nov., isolated from an environmental source in Malaysia. Int. J. Syst. Evol. Microbiol. 59: 705– 708.

61. Slack, A. T.,, M. L. Symonds,, M. F. Dohnt,, and L. D. Smythe. 2006. Identification of pathogenic Leptospira species by conventional or real-time PCR and sequencing of the DNA gyrase subunit B encoding gene. BMC Microbiol. 6: 95.

62. Smits, H. L.,, C. K. Eapen,, S. Sugathan,, M. Kuriakose,, M. H. Gasem,, C. Yersin,, D. Sasaki,, B. Pujianto,, M. Vestering,, T. H. Abdoel,, and G. C. Gussenhoven. 2001. Lateral-flow assay for rapid serodiagnosis of human leptospirosis. Clin. Diagn. Lab. Immunol. 8: 166– 169.

63. Smits, H. L.,, M. A. van Der Hoorn,, M. G. Goris,, G. C. Gussenhoven,, C. Yersin,, D. M. Sasaki,, W. J. Terpstra,, and R. A. Hartskeerl. 2000. Simple latex agglutination assay for rapid serodiagnosis of human leptospirosis. J. Clin. Microbiol. 38: 1272– 1275.

64. Smythe, L. D.,, I. L. Smith,, G. A. Smith,, M. F. Dohnt,, M. L. Symonds,, L. J. Barnett,, and D. B. McKay. 2002. A quantitative PCR (TaqMan) assay for pathogenic Leptospira spp. BMC Infect. Dis. 2: 13.

65. Stallman, N. D. 1987. International Committee on Systematic Bacteriology Subcommittee on the Taxonomy of Leptospira. Minutes of the meeting, 5 and 6 September 1986, Manchester, England. Int. J. Syst. Bacteriol. 37: 472– 473.

66. Stimson, A. M. 1907. Note on an organism found in yellow-fever tissue. Public Health Rep. 22: 541.

67. Stoddard, R. A.,, J. E. Gee,, P. P. Wilkins,, K. McCaustland,, and A. R. Hoffmaster. 2009. Detection of pathogenic Leptospira spp. through TaqMan polymerase chain reaction targeting the LipL32 gene. Diagn. Microbiol. Infect. Dis. 64: 247– 255.

68. Suputtamongkol, Y.,, K. Niwattayakul,, C. Suttinont,, K. Losuwanaluk,, R. Limpaiboon,, W. Chierakul,, V. Wuthiekanun,, S. Triengrim,, M. Chenchittikul,, and N. J. White. 2004. An open, randomized, controlled trial of penicillin, doxycycline, and cefotaxime for patients with severe leptospirosis. Clin. Infect. Dis. 39: 1417– 1424.

69. Terpstra, W. J. 1992. Typing leptospira from the perspective of a reference laboratory. Acta Leidensia 60: 79– 87.

70. Thaipadungpanit, J.,, V. Wuthiekanun,, W. Chierakul,, L. D. Smythe,, W. Petkanchanapong,, R. Limpaiboon,, A. Apiwatanaporn,, A. T. Slack,, Y. Suputtamongkol,, N. J. White,, E. J. Feil,, N. P. Day,, and S. J. Peacock. 2007. A dominant clone of Leptospira interrogans associated with an outbreak of human leptospirosis in Thailand. PLoS Neglected Trop. Dis. 1: e56.

71. Turner, L. H. 1968. Leptospirosis II. Serology. Trans. R. Soc. Trop. Med. Hyg. 62: 880– 889.

72. Turner, L. H. 1970. Leptospirosis III. Maintenance, isolation and demonstration of leptospires. Trans. R. Soc. Trop. Med. Hyg. 64: 623– 646.

73. Uip, D. E.,, V. Amato Neto,, and M. S. Duarte. 1992. Diagnóstico precoce da leptospirose por demonstração de antígenos através de exame imuno-histoquímino em músculo da panturrilha. Rev. Inst. Med. Trop. São Paulo 34: 375– 381.

74. Victoria, B.,, A. Ahmed,, R. L. Zuerner,, N. Ahmed,, D. M. Bulach,, J. Quinteiro,, and R. A. Hartskeerl. 2008. Conservation of the S10-spc-α locus within otherwise highly plastic genomes provides phylogenetic insight into the genus Leptospira. PLoS One 3: e2752.

75. Vijayachari, P.,, A. P. Sugunan,, T. Umapathi,, and S. C. Sehgal. 2001. Evaluation of darkground microscopy as a rapid diagnostic procedure in leptospirosis. Indian J. Med. Res. 114: 54– 58.

76. Ward, M. P.,, L. F. Guptill,, A. Prahl,, and C. C. Wu. 2004. Serovar-specific prevalence and risk factors for leptospirosis among dogs: 90 cases (1997-2002). J. Am. Vet. Med. Assoc. 224: 1958– 1963.

77. Winslow, W. E.,, D. J. Merry,, M. L. Pirc,, and P. L. Devine. 1997. Evaluation of a commercial enzyme-linked immunosorbent assay for detection of immunoglobulin M antibody in diagnosis of human leptospiral infection. J. Clin. Microbiol. 35: 1938– 1942.

78. World Health Organization. 2003. Human Leptopirosis: Guidance for Diagnosis, Surveillance and Control. World Health Organization, Geneva, Switzerland.

79. World Health Organization. 1999. Leptospirosis worldwide, 1999. Wkly. Epidemiol. Rec. 74: 237– 242.

80. Wylie, J. A. H.,, and E. Vincent. 1947. The sensitivity of organisms of the genus Leptospira to penicillin and streptomycin. J. Pathol. Bacteriol. 59: 247– 254.

81. Yasuda, P. H.,, A. G. Steigerwalt,, K. R. Sulzer,, A. F. Kaufmann,, F. Rogers,, and D. J. Brenner. 1987. Deoxyribonucleic acid relatedness between serogroups and serovars in the family Leptospiraceae with proposals for seven new Leptospira species. Int. J. Syst. Bacteriol. 37: 407– 415.

82. Zaki, S. R.,, W.-J. Shieh, and The Epidemic Working Group. 1996. Leptospirosis associated with outbreak of acute febrile illness and pulmonary haemorrhage, Nicaragua, 1995. Lancet 347: 535.

Tables

Leptospira

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TABLE 1

Serogroups and selected serovars of Leptospira interroganssensu lato

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TABLE 1

Serogroups and selected serovars of Leptospira interroganssensu lato

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TABLE 2

Genospecies of Leptospira

a These species comprise the clade of pathogenic Leptospira species.

b Currently only nonpathogenic strains of these species are known.

c These species comprise the clade of Leptospira species intermediate between the pathogenic and nonpathogenic clades ( Fig. 1 ).

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TABLE 2

Genospecies of Leptospira

a These species comprise the clade of pathogenic Leptospira species.

b Currently only nonpathogenic strains of these species are known.

c These species comprise the clade of Leptospira species intermediate between the pathogenic and nonpathogenic clades ( Fig. 1 ).

/content/10.1128/9781555816728.chap55.tab55-3

TABLE 3

Leptospiral serovars found in multiple species a

a Based on data reported by Brenner et al. ( 6 ) and by Feresu et al. ( 21 ).

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TABLE 3

Leptospiral serovars found in multiple species a

a Based on data reported by Brenner et al. ( 6 ) and by Feresu et al. ( 21 ).