Chapter 82 : Parainfluenza and Mumps Viruses

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The human parainfluenza viruses (HPIVs) and mumps virus are members of the family. HPIV-1, -2, and -3 are associated with upper respiratory tract infections in infants, children, and adults. Only respiratory syncytial virus (RSV) causes more lower respiratory tract infections in neonates and young infants than HPIV-3. The indirect fluorescent-antibody (IFA) staining test is appropriate for the same types of samples as for the direct fluorescent-antibody (DFA) staining kit, with the same smear preparation and fixation guidelines. Confirmatory testing is routinely completed by immuno-fluorescence techniques involving the use of HPIV MAbs in DFA or IFA assays, as described for HPIV antigen detection in clinical samples. An overview of the recommended protocol from the manufacturer of R-Mix is shown in "Parainfluenza Viruses" under "Isolation and Identification." As mumps virus infections in previously vaccinated individuals result in decreased levels of virus shedding into the buccal cavity, virus isolation may be difficult. Given the potential lack of serologic test specificity, virus isolation and RNA detection in clinical samples remain the most effective ways to confirm infection in unvaccinated individuals.

Citation: Leland D. 2011. Parainfluenza and Mumps Viruses, p 1347-1356. In Versalovic J, Carroll K, Funke G, Jorgensen J, Landry M, Warnock D (ed), Manual of Clinical Microbiology, 10th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816728.ch82
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Image of FIGURE 1

Immunofluorescence staining of HPIV-infected R-Mix Too and mumps virus-infected R-Mix cell cultures. R-Mix Too cells were infected with HPIV-1 (A), HPIV-2 (B), or HPIV-3 (C). (D) R-Mix cells infected with mumps virus. Magnification, ×200 (all). (A to C) Courtesy of Indiana Pathology Images; (D) courtesy of Diagnostic Hybrids, Inc., Athens, OH.

Citation: Leland D. 2011. Parainfluenza and Mumps Viruses, p 1347-1356. In Versalovic J, Carroll K, Funke G, Jorgensen J, Landry M, Warnock D (ed), Manual of Clinical Microbiology, 10th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816728.ch82
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1. Ansari, S. A.,, V. S. Springthorpe,, S. A. Sattar,, S. Rivard,, and M. Rahman. 1991. Potential role of hands in the spread of respiratory viral infections: studies with human parainfluenza virus 3 and rhinovirus. J. Clin. Microbiol. 29: 2115 2119.
2. Bellini, W. J.,, J. P. Icenogle,, and J. L. Sever,. 2009. Measles, mumps, and rubella, p. 562 577. In S. Specter,, R. L. Hodinka,, S. A. Young,, and D. L. Wiedbrauk (ed.), Clinical Virology Manual, 4th ed. ASM Press, Washington, DC.
3. Bitsko, R. H.,, M. M. Cortese,, G. H. Dayan,, P.A. Rota,, L. Lowe,, S. C. Iversen,, and W. J. Bellini. 2008. Detection of RNA of mumps virus during an outbreak in a population with a high level of measles, mumps, and rubella vaccine coverage. J. Clin. Microbiol. 46: 1101 1103.
4. Boddicker, J. D.,, P. A. Rota,, T. Kreman,, A. Wangeman,, L. Lowe,, K. B. Hummel,, R. Thompson,, W. J. Bellini,, M. Pentella,, and L. E. DesJardin. 2007. Real-time reverse transcription-PCR assay for detection of mumps virus RNA in clinical specimens. J. Clin. Microbiol. 45: 2902 2908.
5. Boyd, J. F.,, and V. Vince-Ribaric. 1973. The examination of cerebrospinal fluid cells by fluorescent antibody staining to detect mumps antigen. Scand. J. Infect. Dis. 5: 7 15.
6. Cazacu, A. C.,, S. E. Chung,, J. Greer,, and G. J. Demmler. 2004. Comparison of the Directigen Flu A+B membrane enzyme immunoassay with viral culture for rapid detection of influenza A and B viruses in respiratory specimens. J. Clin. Microbiol. 42: 3707 3710.
7. Centers for Disease Control and Prevention. 1998. Measles, mumps, and rubella-vaccine use and strategies for elimination of measles, rubella, and congenital rubella syndrome and control of mumps: recommendation of the Advisory Committee on Immunization Practices (ACIP). Morb. Mortal. Wkly. Rep. 47( RR-8): 616.
8. Chakrabarti, S.,, K. E. Collingham,, K. Holder,, S. Oyaide,, D. Pillay,, and D. W. Milligan. 2000. Parainfluenza virus type 3 infections in hematopoietic stem cell transplant recipients: response to ribavirin therapy. Clin. Infect. Dis. 31: 1516 1518.
9. Chonmaitree, T.,, and K. J. Henrickson. 2000. Detection of respiratory viruses in the middle ear fluids of children with acute otitis media by multiplex reverse transcription: polymerase chain reaction assay. Pediatr. Infect. Dis. J. 19: 258 260.
10. Condorelli, F.,, G. Scalia,, A. Stivala,, R. Gallo,, A. Marino,, C. M. Battaglini,, and A. Castro. 1994. Detection of immunoglobulin G to measles virus, rubella virus, and mumps virus in serum samples and in microquantities of whole blood dried on filter paper. J. Virol. Methods 49: 25 36.
11. Davidkin, I.,, S. Jokinen,, A. Paananen,, P. Leinikki,, and H. Peltola. 2005. Etiology of mumps-like illnesses in children and adolescents vaccinated for measles, mumps, and rubella. J. Infect. Dis. 1919: 719 723.
12. Dayan, G. H.,, M. P. Quinlisk,, A. A. Parker,, A. E. Barskey,, M. L. Harris,, J. M. Hill Schwartz,, K. Hunt,, C. G. Finley,, D. P. Leschinsky,, A. L. O’Keefe,, J. Clayton,, L. K. Kightlinger,, E. G. Dietle,, J. Berg,, C. L. Kenyon,, S. T. Goldstein,, S. K. Stokley,, S. B. Redd,, P. A Rota,, J. Rota,, D. Bi,, S. W. Roush,, C. B. Bridges,, T. A. Santibanez,, U. Parashar,, W. J. Bellini,, and J. F. Seward. 2008. Recent resurgence of mumps in the United States. N. Engl. J. Med. 358: 1580 1589.
13. Dunn, J. J.,, R. D. Woolstenhulme,, J. Langer,, and K. C. Carroll. 2004. Sensitivity of respiratory virus culture when screening with R-Mix fresh cells. J. Clin. Microbiol. 42: 79 82.
14. Elizaga, J.,, E. Olavarria,, J. Apperley,, J. Goldman,, and K. Ward. 2001. Parainfluenza virus 3 infection after stem cell transplant: relevance to outcome of rapid diagnosis and ribavirin treatment. Clin. Infect. Dis. 32: 413 418.
15. Fan, J.,, and K. J. Henrickson. 1996. Rapid diagnosis of human parainfluenza virus type 1 infection by quantitative reverse transcription-PCR-enzyme hybridization assay. J. Clin. Microbiol. 34: 1914 1917.
16. Fan, J.,, K. J. Henrickson,, and L. L. Savatski. 1998. Rapid simultaneous diagnosis of infections with respiratory syncytial viruses A and B, influenza viruses A and B, and human parainfluenza virus types 1, 2, and 3 by multiplex quantitative reverse transcription-polymerase chain reaction-enzyme hybridization assay (Hexaplex). Clin. Infect. Dis. 26: 1397 1402.
17. Germann, D.,, M. Gorgievski,, A. Strohle,, and L. Matter. 1998. Detection of mumps virus in clinical specimens by rapid centrifugation culture and conventional tube cell culture. J. Virol. Methods 73: 59 64.
18. Griffin, M. R.,, F. J. Walker,, M. K. Iwane,, G. A. Weinberg,, M. A. Staat,, and D. D. Erdman. 2004. Epidemiology of respiratory infections in young children: insights from the new vaccine surveillance network. Pediatr. Infect. Dis. J. 23: S188 S192.
19. Henrickson, K. J. 2003. Parainfluenza viruses. Clin. Microbiol. Rev. 16: 242 264.
20. Henrickson, K. J. 2004. Advances in the laboratory diagnosis of viral respiratory disease. Pediatr. Infect. Dis. J. 23: S6 S10.
21. Henrickson, K. J. 2005. Cost-effective use of rapid diagnostic techniques in the treatment and prevention of viral respiratory infections. Pediatr. Ann. 34: 24 31.
22. Henrickson, K. J.,, S. Hoover,, K. S. Kehl,, and J. Weimin. 2004. National disease burden of respiratory viruses detected in children by polymerase chain reaction. Pediatr. Infect. Dis. J. 23: S11 S18.
23. Hohenthal, U.,, J. Nikoskelainer,, R. Vainionpaa,, R. Peltonen,, M. Routamaa,, M. Itala,, and P. Kotilainen. 2001. Parainfluenza virus type 3 infections in a hematology unit. Bone Marrow Transplant. 27: 295 300.
24. Iwane, M. K.,, K. M. Edwards,, P. G. Szilagyi,, F. J. Walker,, M. R. Griffin,, G. A. Weinberg,, C. Coulen,, K. A. Poehling,, L. P. Shone,, S. Balter,, C. B. Hall,, D. D. Erdman,, J. Wooten,, and B. Schwartz. 2004. Population-based surveillance for hospitalizations associated with respiratory syncytial virus, influenza virus, and parainfluenza viruses among young children. Pediatrics 113: 1758 1764.
25. Jin, L.,, Y. Feng,, R. Parry,, A. Cui,, and Y. Lu. 2007. Realtime PCR and its application to mumps rapid diagnosis. J. Med. Virol. 79: 1761 1767.
26. Kehl, S. C.,, K. J. Henrickson,, W. Hua,, and J. Fan. 2001. Evaluation of the Hexaplex assay for detection of respiratory viruses in children. J. Clin. Microbiol. 39: 1696 1701.
27. Knowles, W. A.,, and B. J. Cohen. 2001. Efficient isolation of mumps virus from a community outbreak using the marmoset lymphoblastoid cell line B95a. J. Virol. Methods 96: 93 96.
28. Landry, M. L.,, and D. Ferguson. 2000. SimulFluor respiratory screen for rapid detection of multiple respiratory viruses in clinical specimens by immunofluorescence staining. J. Clin. Microbiol. 38: 708 711.
29. Leland, D. S. 1996. Clinical Virology. W. B. Saunders, Co., Philadelphia, PA.
30. Leland, D. S., 2002. Measles and mumps, p. 683 686. In N. R. Rose,, R. G. Hamilton,, and B. Detrick (ed.), Manual of Clinical Laboratory Immunology, 6th ed. ASM Press, Washington, DC.
31. Leung, A. K.,, J. D. Kellner,, and D. W. Johnson. 2004. Viral croup: a current prospective. J. Pediatr. Health Care 18: 297 301.
32. Lindeman, J.,, W. K. Muller,, J. Versteeg,, G. T. A. M. Bots,, and A. C. B. Peters. 1974. Rapid diagnosis of meningoencephalitis, encephalitis. Immunofluorescent examination of fresh and in vitro cultured cerebrospinal fluid cells. Neurology 24: 143 148.
33. Madden, J. F.,, J. L. Burchette, Jr.,, and L. P. Hale. 2004. Pathology of parainfluenza virus infection in patients with congenital immunodeficiency syndromes. Hum. Pathol. 35: 594 603.
34. Marx, A.,, H. E. Gary, Jr.,, B. J. Marston,, D. D. Erdman,, R. F. Breiman,, T. J. Torok,, J. F. Plouffe,, T. M. File, Jr.,, and L. J. Anderson. 1999. Parainfluenza virus infection among adults hospitalized for lower respiratory tract infection. Clin. Infect. Dis. 29: 134 140.
35. McAdam, A. J.,, and A. H. Sharpe,. 2005. Infectious diseases, p. 343 414. In V. Kumar,, A. K. Abbas,, and N. Fausto (ed.), Robbins and Cotran Pathologic Basis of Disease, 7th ed. Elsevier Saunders, Philadelphia, PA.
36. Merante, F.,, S. Yaghoubian,, and R. Janeczko. 2007. Principles of the xTAG™ respiratory viral panel assay (RVP assay). J. Clin. Virol. 40( Suppl. 1): S31 S35.
37. Minnich, L. L.,, and G. C. Ray. 1987. Early testing of cell cultures for detection of hemadsorbing viruses. J. Clin. Microbiol. 25: 421 422.
38. Ono, N.,, H. Tatsuo,, Y. Hidaka,, T. Aoki,, H. Minagawa,, and Y. Yanagi. 2001. Measles viruses on throat swabs from measles patients use signaling-lymphocytic activation molecule (CDw150) but not CD46 as a cellular receptor. J. Virol. 75: 4399 4401.
39. Pabbaraju, K.,, K. L. Tokaryk,, S. Wong,, and J. D. Fox. 2008. Comparison of the Luminex xTAG respiratory viral panel with in-house nucleic acid amplification tests for diagnosis of respiratory virus infections. J. Clin. Microbiol. 46: 3056 3062.
40. Palacios, G.,, O. Jabado,, D. Cisterna,, F. DeOry,, N. Renwick,, J. E. Echevarria,, A. Castellanos,, M. Mosquera,, M. C. Friere,, R. H. Campos,, and W. I. Lipkin. 2005. Molecular identification of mumps virus genotypes from clinical samples: standardized method of analysis. J. Clin. Microbiol. 43: 1869 1878.
41. Poggio, G. P.,, D. Rodriguez,, D. Cisterna,, M. C. Freire,, and J. Cello. 2000. Nested PCR for rapid detection of mumps virus in cerebrospinal fluid from patients with neurological diseases. J. Clin. Microbiol. 38: 274 278.
42. Reina, J.,, F. Ballesteros,, M. Mari,, and M. Munar. 2001. Evaluation of different continuous cell lines in the isolation of mumps virus by the shell vial method from clinical samples. J. Clin. Pathol. 54: 924 926.
43. Reina, J.,, F. Ballesteros,, E. Ruiz de Gopegui,, M. Munar,, and M. Mari. 2003. Comparison between indirect immunofluorescence assay and shell vial culture for detection of mumps virus from clinical samples. J. Clin. Microbiol. 41: 5186 5187.
44. Shima, T.,, G. Yoshimoto,, A. Nonami,, S. Yoshida,, K. Kamezaki,, H. Iwasaki,, K. Takenaka,, T. Miyamoto,, N. Harada,, T. Teshima,, K. Akashi,, and K. Nagafuji. 2008. Successful treatment of parainfluenza virus 3 pneumonia with oral ribavirin and methylprednisolone in a bone marrow transplant recipient. Int. J. Hematol. 88: 336 340.
45. Templeton, K. E.,, S. A. Scheltinga,, M. F. C. Beersman,, A. C. M. Kroes,, and E. C. J. Claas. 2004. Rapid and sensitive method using multiplex real-time PCR for diagnosis of infections by influenza A and influenza B viruses, respiratory syncytial virus, and parainfluenza viruses 1, 2, 3, and 4. J. Clin. Microbiol. 42: 1564 1569.
46. Waner, J. L.,, and E. M. Swierkosz,. 2003. Mumps and parainfluenza viruses, p. 1368 1377. In P. R. Murray,, E. J. Baron,, J. H. Jorgensen,, M. A. Pfaller,, and R. H. Yolken (ed.), Manual of Clinical Microbiology, 8th ed., vol. 2. ASM Press, Washington, DC.


Generic image for table

Diagnostic methods for parainfluenza and mumps virus detection

IF, immunofluorescence.

Citation: Leland D. 2011. Parainfluenza and Mumps Viruses, p 1347-1356. In Versalovic J, Carroll K, Funke G, Jorgensen J, Landry M, Warnock D (ed), Manual of Clinical Microbiology, 10th Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816728.ch82

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