Insect Cell Culture

Spiros N. Agathos

doi:10.1128/9781555816827.ch15

Chapter 15 : Insect Cell Culture

Author: Spiros N. Agathos

Content Type: Reference

Publication Year: 2010

Category: Applied and Industrial Microbiology

Book DOI: 10.1128/9781555816827

Chapter DOI: 10.1128/9781555816827.ch15

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Abstract:

The insect cell-baculovirus expression vector system (IC-BEVS) is a highly versatile system because it can express gene products of practically any origin (from bacteria to human tissue), and in contrast to most industrial mammalian cell culture systems, it is based on engineering only the vector and not the host cell line. As a result, the development time needed to progress from gene cloning to protein overproduction is much shorter. Compared to other biomanufacturing platforms, the IC-BEVS offers other significant advantages, including typically high product titers, a range of posttranslational modifications, and the possibility to express multimeric proteins or even several distinct proteins using the same vector. The Sf lines are adapted to suspension cultivation and are easily detached from T-flask (or other recipient) surfaces by gentle agitation without trypsinization. Small bench-scale culture involves volumes ranging from a total of a few milliliters in wells of multiple-well plates or T-flasks for adherent culture to shake flasks or spinners up to 250 ml for suspension culture. A number of bioreactor systems have been investigated and proven merits of bioreactors in terms of robustness and scalability. Batch culture remains the most common method for large scale IC-BEVS processing because of its inherent simplicity and flexibility in bioreactor equipment. The practice of insect cell culture is becoming more and more widespread as an essential component of the IC-BEVS, perhaps one of the most successful and far-reaching expression systems available today for the production of recombinant proteins and viral particles.

Citation: Agathos S. 2010. Insect Cell Culture, p 212-222. In Baltz R, Demain A, Davies J, Bull A, Junker B, Katz L, Lynd L, Masurekar P, Reeves C, Zhao H (ed), Manual of Industrial Microbiology and Biotechnology, Third Edition. ASM Press, Washington, DC. doi: 10.1128/9781555816827.ch15

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References

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Tables

Insect Cell Culture

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/content/10.1128/9781555816827.ch15.ch15tab01

TABLE 1

Formulation of YSD, a generic all-purpose serum-free medium for insect cell culture

TABLE 1

Formulation of YSD, a generic all-purpose serum-free medium for insect cell culture