Molecular Detection of Respiratory Viruses

Richard S. Buller; Max Q. Arens

doi:10.1128/9781555816834.ch39

Chapter 39 : Molecular Detection of Respiratory Viruses

Authors: Richard S. Buller¹, Max Q. Arens²

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Affiliations: 1: Department of Pediatrics, Washington University School of Medicine, St. Louis, MO 63110; 2: Department of Pediatrics, Washington University School of Medicine, St. Louis, MO 63110

Content Type: Reference

Publication Year: 2011

Category: Clinical Microbiology; Bacterial Pathogenesis

Book DOI: 10.1128/9781555816834

Chapter DOI: 10.1128/9781555816834.ch39

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Abstract:

This chapter deals with current assays that are commercially available or noncommercial assays that have been well characterized in peer-reviewed literature. Methods for the molecular detection of respiratory viruses have, depending on the capabilities and resources available to the laboratory, a variety of potential clinical applications. Molecular amplification assays for the detection of rhinovirus RNA in clinical specimens often target the 5’ NCR, which contains sequences conserved across the 100 rhinovirus serotypes. In addition to advances in technology, another important step for the future will be the performance of clinical trials to prove the clinical usefulness of respiratory virus panel (RVP) results. Laboratorians and physicians will have to come to terms with co- and multiple infections. Molecular detection of respiratory viruses is currently a rapidly changing field and will likely continue to be for the near future. The good news is that more information about the many and varied causes of respiratory illness are currently acquired and that this information will be an important part of the puzzle in advancing human health.

Citation: Buller R, Arens M. 2011. Molecular Detection of Respiratory Viruses, p 604-630. In Persing D, Tenover F, Tang Y, Nolte F, Hayden R, van Belkum A (ed), Molecular Microbiology. ASM Press, Washington, DC. doi: 10.1128/9781555816834.ch39

Key Concept Ranking

Upper Respiratory Tract Infections: 0.5453491
Lower Respiratory Tract Infections: 0.54085356
Human respiratory syncytial virus: 0.5403188
Human parainfluenza virus 1: 0.48972037
Human parainfluenza virus 2: 0.48972037
Human parainfluenza virus 4: 0.48972037

0.5453491

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References

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Tables

Molecular Detection of Respiratory Viruses

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/content/10.1128/9781555816834.ch39.ch39tab01

TABLE 1

Taxonomy of viruses causing human respiratory disease

TABLE 1

Taxonomy of viruses causing human respiratory disease

/content/10.1128/9781555816834.ch39.ch39tab02

TABLE 2

Distribution of types among the human adenovirus species

TABLE 2

Distribution of types among the human adenovirus species

/content/10.1128/9781555816834.ch39.ch39tab03

TABLE 3

Nucleic acid amplification assays for the detection of adenoviruses

TABLE 3

Nucleic acid amplification assays for the detection of adenoviruses

/content/10.1128/9781555816834.ch39.ch39tab04

TABLE 4

Nucleic amplification assays for the detection of influenza viruses

TABLE 4

Nucleic amplification assays for the detection of influenza viruses

/content/10.1128/9781555816834.ch39.ch39tab05

TABLE 5

Nucleic acid amplification assays for the detection of RSV

TABLE 5

Nucleic acid amplification assays for the detection of RSV

/content/10.1128/9781555816834.ch39.ch39tab06

TABLE 6

Nucleic acid amplification assays for the detection of HMPV

TABLE 6

Nucleic acid amplification assays for the detection of HMPV