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Chapter 3 : Phospholipid Fatty Acid Stable Isotope Probing Techniques in Microbial Ecology
Authors:
P. J. Maxfield1,
R. P. Evershed2
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Affiliations:
1: Organic Geochemistry Unit, Bristol Biogeochemistry Research Centre, School of Chemistry, University of Bristol, Cantock's Close, Bristol BS8 1TS, United Kingdom;
2: Organic Geochemistry Unit, Bristol Biogeochemistry Research Centre, School of Chemistry, University of Bristol, Cantock's Close, Bristol BS8 1TS, United Kingdom
Content Type: Monograph
Publication Year:
2011
Category: Environmental Microbiology; Applied and Industrial Microbiology
Book DOI:
10.1128/9781555816896
Chapter DOI:
10.1128/9781555816896.ch3
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Abstract:
This chapter reviews the methodologies used to present stable isotopically labeled substrates to microbial communities present in a wide range of environmental materials, and also discusses the wet chemical and instrumental methods used to determine compound-specific δ13C values of individual phospholipid fatty acid (PLFA). The different ways in which the δ 13C values obtained can be used to assess a variety of properties and activities of microbial communities in the environment are discussed in this chapter. The most widely used labeling techniques for PLFA- stable isotope probing (SIP) utilize 13C-enriched gases as substrates. The PLFA-SIP approach significantly extends conventional PLFA profiling methods by identifying PLFAs diagnostic of specific functional groups through their enhanced 13C signatures derived from the assimilation of applied 13C-substrate(s). In this respect, the approach has key resonances with current trends in the way that environmental microbial communities are being considered in terms of the functioning and stability of ecosystems, especially in relation to the importance of ecosystem services in the context of sustainable environments. The relative ease of preparing PLFA fatty acid methyl esters (FAMEs), combined with the high sensitivity of the gas chromatography combustion-isotope ratio mass spectrometry (GC-C-IRMS) method, makes PLFA-SIP an extremely robust methodology, which allows very large numbers of environmental samples to be studied.
Citation:
Maxfield P, Evershed R.
2011.
Phospholipid Fatty Acid Stable Isotope Probing Techniques in Microbial Ecology,
p 37-71.
In
Murrell J, Whiteley A (ed),
Stable Isotope Probing and Related Technologies.
ASM Press, Washington, DC.
doi: 10.1128/9781555816896.ch3
Key Concept Ranking
- Microbial Ecology
- 0.7300024
- Microbial Communities in Environment
- 0.51572007
- Acetyl Coenzyme A
- 0.50581396
- Cell Wall Components
- 0.42218503
- Bacterial Cell Wall
- 0.40975738
0.7300024
Figures
Phospholipid Fatty Acid Stable Isotope Probing Techniques in Microbial Ecology
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Citation:
Maxfield P, Evershed R.
2011.
Phospholipid Fatty Acid Stable Isotope Probing Techniques in Microbial Ecology,
p 37-71.
In
Murrell J, Whiteley A (ed),
Stable Isotope Probing and Related Technologies.
ASM Press, Washington, DC.
doi: 10.1128/9781555816896.ch3
/content/10.1128/9781555816896.ch03.ch03fig01
FIGURE 1.
General structure of a phospholipid. Sn denotes the C number position on the glycerol backbone. R1 and R2 represent long hydrocarbon chains. × represents one of the various substituents listed in
Table 1
.
10.1128/9781555816896/f0038-01_thmb.gif
10.1128/9781555816896/f0038-01.gif
FIGURE 1.
General structure of a phospholipid. Sn denotes the C number position on the glycerol backbone. R1 and R2 represent long hydrocarbon chains. × represents one of the various substituents listed in Table 1 .
Citation:
Maxfield P, Evershed R.
2011.
Phospholipid Fatty Acid Stable Isotope Probing Techniques in Microbial Ecology,
p 37-71.
In
Murrell J, Whiteley A (ed),
Stable Isotope Probing and Related Technologies.
ASM Press, Washington, DC.
doi: 10.1128/9781555816896.ch3
/content/10.1128/9781555816896.ch03.ch03fig02
FIGURE 2.
Pictorial representation of a cross section through a cell membrane displaying the phospholipid bilayer with integral (a) and peripheral (b) membrane proteins.
10.1128/9781555816896/f0038-02_thmb.gif
10.1128/9781555816896/f0038-02.gif
FIGURE 2.
Pictorial representation of a cross section through a cell membrane displaying the phospholipid bilayer with integral (a) and peripheral (b) membrane proteins.
Citation:
Maxfield P, Evershed R.
2011.
Phospholipid Fatty Acid Stable Isotope Probing Techniques in Microbial Ecology,
p 37-71.
In
Murrell J, Whiteley A (ed),
Stable Isotope Probing and Related Technologies.
ASM Press, Washington, DC.
doi: 10.1128/9781555816896.ch3
/content/10.1128/9781555816896.ch03.ch03fig03
FIGURE 3.
13CO2 pulse labeling plant microcosm (adapted from
Nakano-Hylander and Olsson, 2007
).
10.1128/9781555816896/f0042-01_thmb.gif
10.1128/9781555816896/f0042-01.gif
FIGURE 3.
13CO2 pulse labeling plant microcosm (adapted from Nakano-Hylander and Olsson, 2007 ).
Citation:
Maxfield P, Evershed R.
2011.
Phospholipid Fatty Acid Stable Isotope Probing Techniques in Microbial Ecology,
p 37-71.
In
Murrell J, Whiteley A (ed),
Stable Isotope Probing and Related Technologies.
ASM Press, Washington, DC.
doi: 10.1128/9781555816896.ch3
/content/10.1128/9781555816896.ch03.ch03fig04
FIGURE 4.
Static flux chamber (a) and continuous-flow flux chamber (b).
10.1128/9781555816896/f0043-01_thmb.gif
10.1128/9781555816896/f0043-01.gif
FIGURE 4.
Static flux chamber (a) and continuous-flow flux chamber (b).
Citation:
Maxfield P, Evershed R.
2011.
Phospholipid Fatty Acid Stable Isotope Probing Techniques in Microbial Ecology,
p 37-71.
In
Murrell J, Whiteley A (ed),
Stable Isotope Probing and Related Technologies.
ASM Press, Washington, DC.
doi: 10.1128/9781555816896.ch3
/content/10.1128/9781555816896.ch03.ch03fig05
FIGURE 5.
Freshwater sediment core incubation setup (adapted from
Deines et al., 2007
).
10.1128/9781555816896/f0044-01_thmb.gif
10.1128/9781555816896/f0044-01.gif
FIGURE 5.
Freshwater sediment core incubation setup (adapted from Deines et al., 2007 ).
Citation:
Maxfield P, Evershed R.
2011.
Phospholipid Fatty Acid Stable Isotope Probing Techniques in Microbial Ecology,
p 37-71.
In
Murrell J, Whiteley A (ed),
Stable Isotope Probing and Related Technologies.
ASM Press, Washington, DC.
doi: 10.1128/9781555816896.ch3
/content/10.1128/9781555816896.ch03.ch03fig06
FIGURE 6.
Continuous-flow gas diffusion core incubator schematic.
10.1128/9781555816896/f0044-02_thmb.gif
10.1128/9781555816896/f0044-02.gif
FIGURE 6.
Continuous-flow gas diffusion core incubator schematic.
Citation:
Maxfield P, Evershed R.
2011.
Phospholipid Fatty Acid Stable Isotope Probing Techniques in Microbial Ecology,
p 37-71.
In
Murrell J, Whiteley A (ed),
Stable Isotope Probing and Related Technologies.
ASM Press, Washington, DC.
doi: 10.1128/9781555816896.ch3
/content/10.1128/9781555816896.ch03.ch03fig07
FIGURE 7.
Partial gas chromatogram of the PLFA fraction of Bronydd Mawr upland grassland soil following derivatization with BF3 methanol. Separation was achieved using a Varian Factor Four VF23 ms (high cyanopropyl modified methyl polysiloxane) fused silica column (60 m by 0.32 mm internal diameter [ID]; 0.15-mm film thickness). The carrier gas was hydrogen, and the oven temperature was programmed from 50°C (held for 2 min) to 100°C at 15°C min-1, from 100 to 220°C at 4°C min-1, and from 220 to 240°C (held for 5 min) at 15°C min-1. PLFA assignments: 1 = C19 alkane, 2 = 14:0, 3 = i15:0, 4 = a15:0, 5 = 15:0, 6 = i16:0, 7 = 16:0, 8 = 16:1ω11, 9 = br17:0, 10 = 16:1ω7, 11 = i17:0 & 16:1ω5, 12 = a17:0, 13 = 17:1ω8, 14 = 17:0, 15 = 18:0, 16 = cyc19:0, 17 = 18:1ω9c, 18 = 18:1ω7c, 19 = 18:1ω5, 20 = 18:2ω3,6 (
Maxfield et al., 2006
).
10.1128/9781555816896/f0048-01_thmb.gif
10.1128/9781555816896/f0048-01.gif
FIGURE 7.
Partial gas chromatogram of the PLFA fraction of Bronydd Mawr upland grassland soil following derivatization with BF3 methanol. Separation was achieved using a Varian Factor Four VF23 ms (high cyanopropyl modified methyl polysiloxane) fused silica column (60 m by 0.32 mm internal diameter [ID]; 0.15-mm film thickness). The carrier gas was hydrogen, and the oven temperature was programmed from 50°C (held for 2 min) to 100°C at 15°C min-1, from 100 to 220°C at 4°C min-1, and from 220 to 240°C (held for 5 min) at 15°C min-1. PLFA assignments: 1 = C19 alkane, 2 = 14:0, 3 = i15:0, 4 = a15:0, 5 = 15:0, 6 = i16:0, 7 = 16:0, 8 = 16:1ω11, 9 = br17:0, 10 = 16:1ω7, 11 = i17:0 & 16:1ω5, 12 = a17:0, 13 = 17:1ω8, 14 = 17:0, 15 = 18:0, 16 = cyc19:0, 17 = 18:1ω9c, 18 = 18:1ω7c, 19 = 18:1ω5, 20 = 18:2ω3,6 ( Maxfield et al., 2006 ).
Citation:
Maxfield P, Evershed R.
2011.
Phospholipid Fatty Acid Stable Isotope Probing Techniques in Microbial Ecology,
p 37-71.
In
Murrell J, Whiteley A (ed),
Stable Isotope Probing and Related Technologies.
ASM Press, Washington, DC.
doi: 10.1128/9781555816896.ch3
/content/10.1128/9781555816896.ch03.ch03fig08
FIGURE 8.
Partial gas chromatogram of the PLFA fraction of Bronydd Mawr upland grassland soil following derivatization with DMDS to separate and identify the unsaturated PLFAs. Separation was achieved with a Chrompack CPSIL5-CB (100% dimethyl polysiloxane) fused silica column (50 m by 0.32 mm ID; 0.12 mm film thickness). The carrier gas was hydrogen and the oven was programmed from 40°C (held for 2 min) to 150°C at 12°C min-1, then 150 to 260°C (held for 5 min) at 4°C min-1. PLFA assignments: 1 = i15:0, 2 = a15:0, 3 = i16:0, 4 = 16:0, 5 = br17:0, 6 = i17:0, 7 = a17:0, 8 = 17:0, 9 = 18:0, 10 = 19:0, 11 = cyc19:0, 12 = 18:1ω13, 13 = 15:1ω11, 14 = 16:1ω12, 15 = 18:1ω7, 16 = 16:1ω11 & 16:1ω9, 17 = 16:1ω7, 18 = 16:1ω5, 19 = 17:1ω8, 20 = 18:1ω9c, 21 = 18:1ω7c, 22 = 18:1ω5, 23 = 19:1ω6, 24 = 19:1ω7. All unsaturated compounds were analyzed as DMDS derivatives.
10.1128/9781555816896/f0049-01_thmb.gif
10.1128/9781555816896/f0049-01.gif
FIGURE 8.
Partial gas chromatogram of the PLFA fraction of Bronydd Mawr upland grassland soil following derivatization with DMDS to separate and identify the unsaturated PLFAs. Separation was achieved with a Chrompack CPSIL5-CB (100% dimethyl polysiloxane) fused silica column (50 m by 0.32 mm ID; 0.12 mm film thickness). The carrier gas was hydrogen and the oven was programmed from 40°C (held for 2 min) to 150°C at 12°C min-1, then 150 to 260°C (held for 5 min) at 4°C min-1. PLFA assignments: 1 = i15:0, 2 = a15:0, 3 = i16:0, 4 = 16:0, 5 = br17:0, 6 = i17:0, 7 = a17:0, 8 = 17:0, 9 = 18:0, 10 = 19:0, 11 = cyc19:0, 12 = 18:1ω13, 13 = 15:1ω11, 14 = 16:1ω12, 15 = 18:1ω7, 16 = 16:1ω11 & 16:1ω9, 17 = 16:1ω7, 18 = 16:1ω5, 19 = 17:1ω8, 20 = 18:1ω9c, 21 = 18:1ω7c, 22 = 18:1ω5, 23 = 19:1ω6, 24 = 19:1ω7. All unsaturated compounds were analyzed as DMDS derivatives.
Citation:
Maxfield P, Evershed R.
2011.
Phospholipid Fatty Acid Stable Isotope Probing Techniques in Microbial Ecology,
p 37-71.
In
Murrell J, Whiteley A (ed),
Stable Isotope Probing and Related Technologies.
ASM Press, Washington, DC.
doi: 10.1128/9781555816896.ch3
/content/10.1128/9781555816896.ch03.ch03fig09
FIGURE 9.
Mass spectra of monounsaturated C18 fatty acid DMDS derivatives (18:1ω7 and 18:1ω9) extracted from Bronydd Mawr upland grassland soil. The fragment ions denoted D, E, and F are used to determine the position of the double bonds in the original PLFA.
10.1128/9781555816896/f0050-01_thmb.gif
10.1128/9781555816896/f0050-01.gif
FIGURE 9.
Mass spectra of monounsaturated C18 fatty acid DMDS derivatives (18:1ω7 and 18:1ω9) extracted from Bronydd Mawr upland grassland soil. The fragment ions denoted D, E, and F are used to determine the position of the double bonds in the original PLFA.
Citation:
Maxfield P, Evershed R.
2011.
Phospholipid Fatty Acid Stable Isotope Probing Techniques in Microbial Ecology,
p 37-71.
In
Murrell J, Whiteley A (ed),
Stable Isotope Probing and Related Technologies.
ASM Press, Washington, DC.
doi: 10.1128/9781555816896.ch3
/content/10.1128/9781555816896.ch03.ch03fig10
FIGURE 10.
Mass spectra of picolinyl esters of (a) straight-chain 17:0 fatty acid and (b) 10Me16:0 fatty acid. Note loss of ion at m/z 262 in panel b, associated with branching at position 7 on the aliphatic C chain.
10.1128/9781555816896/f0052-01_thmb.gif
10.1128/9781555816896/f0052-01.gif
FIGURE 10.
Mass spectra of picolinyl esters of (a) straight-chain 17:0 fatty acid and (b) 10Me16:0 fatty acid. Note loss of ion at m/z 262 in panel b, associated with branching at position 7 on the aliphatic C chain.
Citation:
Maxfield P, Evershed R.
2011.
Phospholipid Fatty Acid Stable Isotope Probing Techniques in Microbial Ecology,
p 37-71.
In
Murrell J, Whiteley A (ed),
Stable Isotope Probing and Related Technologies.
ASM Press, Washington, DC.
doi: 10.1128/9781555816896.ch3
/content/10.1128/9781555816896.ch03.ch03fig11
FIGURE 11.
Origin of major fragment ions in the EI mass spectra of picolinyl esters of PLFAs methyl esters.
10.1128/9781555816896/f0052-02_thmb.gif
10.1128/9781555816896/f0052-02.gif
FIGURE 11.
Origin of major fragment ions in the EI mass spectra of picolinyl esters of PLFAs methyl esters.
Citation:
Maxfield P, Evershed R.
2011.
Phospholipid Fatty Acid Stable Isotope Probing Techniques in Microbial Ecology,
p 37-71.
In
Murrell J, Whiteley A (ed),
Stable Isotope Probing and Related Technologies.
ASM Press, Washington, DC.
doi: 10.1128/9781555816896.ch3
/content/10.1128/9781555816896.ch03.ch03fig12
FIGURE 12.
Generalized schematic of a GC-C-IRMS configured for determining δ13C values of individual compounds. Inset (a) details the optimized connection of the fused silica capillary to the combustion reactor. Mixtures of compounds are separated by GC; combusted online, generating CO2 and H2O; H2O is removed; and the CO2 is introduced into an MS equipped with a triple collector comprising three Faraday cups monitoring simultaneously m/z 44, 45, and 46, corresponding to 12C16O2, 13C16O2, and 12C18O16O, respectively. The output currents are amplified and integrated to allow calculation of δ13C values. Reference CO2 and FAs of known δ13C values are utilized to monitor instrument performance and standardize determinations.
10.1128/9781555816896/f0053-01_thmb.gif
10.1128/9781555816896/f0053-01.gif
FIGURE 12.
Generalized schematic of a GC-C-IRMS configured for determining δ13C values of individual compounds. Inset (a) details the optimized connection of the fused silica capillary to the combustion reactor. Mixtures of compounds are separated by GC; combusted online, generating CO2 and H2O; H2O is removed; and the CO2 is introduced into an MS equipped with a triple collector comprising three Faraday cups monitoring simultaneously m/z 44, 45, and 46, corresponding to 12C16O2, 13C16O2, and 12C18O16O, respectively. The output currents are amplified and integrated to allow calculation of δ13C values. Reference CO2 and FAs of known δ13C values are utilized to monitor instrument performance and standardize determinations.
Citation:
Maxfield P, Evershed R.
2011.
Phospholipid Fatty Acid Stable Isotope Probing Techniques in Microbial Ecology,
p 37-71.
In
Murrell J, Whiteley A (ed),
Stable Isotope Probing and Related Technologies.
ASM Press, Washington, DC.
doi: 10.1128/9781555816896.ch3
/content/10.1128/9781555816896.ch03.ch03fig13
FIGURE 13.
The m/z 44 ion current (below) and instantaneous ratio of m/z 45/44 ions (above) recorded for the PLFAs extracted from a soil following incubation with 13CH4.PLFA assignments: 1 = C19 alkane, 2 = 14:0, 3 = i15:0, 4 = a15:0, 5 = 15:0, 6 = i16:0, 7 = 16:0, 8 = 16:1ω11, 9 = br17:0, 10 = 16:1ω7, 11 = i17:0 and 16:1ω5, 12 = a17:0, 13 = 17:1ω8, 14 = 17:0, 15 = i18:0, 16 = 18:0, 17 = cyc19:0, 18 = 18:1ω9c, 19 = 18:1ω7c, 20 = 18:1ω5, 21 = 18:2ω3,6.
10.1128/9781555816896/f0054-01_thmb.gif
10.1128/9781555816896/f0054-01.gif
FIGURE 13.
The m/z 44 ion current (below) and instantaneous ratio of m/z 45/44 ions (above) recorded for the PLFAs extracted from a soil following incubation with 13CH4.PLFA assignments: 1 = C19 alkane, 2 = 14:0, 3 = i15:0, 4 = a15:0, 5 = 15:0, 6 = i16:0, 7 = 16:0, 8 = 16:1ω11, 9 = br17:0, 10 = 16:1ω7, 11 = i17:0 and 16:1ω5, 12 = a17:0, 13 = 17:1ω8, 14 = 17:0, 15 = i18:0, 16 = 18:0, 17 = cyc19:0, 18 = 18:1ω9c, 19 = 18:1ω7c, 20 = 18:1ω5, 21 = 18:2ω3,6.
Citation:
Maxfield P, Evershed R.
2011.
Phospholipid Fatty Acid Stable Isotope Probing Techniques in Microbial Ecology,
p 37-71.
In
Murrell J, Whiteley A (ed),
Stable Isotope Probing and Related Technologies.
ASM Press, Washington, DC.
doi: 10.1128/9781555816896.ch3
/content/10.1128/9781555816896.ch03.ch03eq01
Untitled
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10.1128/9781555816896/e0055-01.gif
Untitled
Citation:
Maxfield P, Evershed R.
2011.
Phospholipid Fatty Acid Stable Isotope Probing Techniques in Microbial Ecology,
p 37-71.
In
Murrell J, Whiteley A (ed),
Stable Isotope Probing and Related Technologies.
ASM Press, Washington, DC.
doi: 10.1128/9781555816896.ch3
/content/10.1128/9781555816896.ch03.ch03eq02
Untitled
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10.1128/9781555816896/e0056-01.gif
Untitled
Citation:
Maxfield P, Evershed R.
2011.
Phospholipid Fatty Acid Stable Isotope Probing Techniques in Microbial Ecology,
p 37-71.
In
Murrell J, Whiteley A (ed),
Stable Isotope Probing and Related Technologies.
ASM Press, Washington, DC.
doi: 10.1128/9781555816896.ch3
/content/10.1128/9781555816896.ch03.ch03eq03
Untitled
10.1128/9781555816896/e0056-02_thmb.gif
10.1128/9781555816896/e0056-02.gif
Untitled
Citation:
Maxfield P, Evershed R.
2011.
Phospholipid Fatty Acid Stable Isotope Probing Techniques in Microbial Ecology,
p 37-71.
In
Murrell J, Whiteley A (ed),
Stable Isotope Probing and Related Technologies.
ASM Press, Washington, DC.
doi: 10.1128/9781555816896.ch3
/content/10.1128/9781555816896.ch03.ch03eq04
Untitled
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Untitled
Citation:
Maxfield P, Evershed R.
2011.
Phospholipid Fatty Acid Stable Isotope Probing Techniques in Microbial Ecology,
p 37-71.
In
Murrell J, Whiteley A (ed),
Stable Isotope Probing and Related Technologies.
ASM Press, Washington, DC.
doi: 10.1128/9781555816896.ch3
/content/10.1128/9781555816896.ch03.ch03eq05
Untitled
10.1128/9781555816896/e0056-04_thmb.gif
10.1128/9781555816896/e0056-04.gif
Untitled
Citation:
Maxfield P, Evershed R.
2011.
Phospholipid Fatty Acid Stable Isotope Probing Techniques in Microbial Ecology,
p 37-71.
In
Murrell J, Whiteley A (ed),
Stable Isotope Probing and Related Technologies.
ASM Press, Washington, DC.
doi: 10.1128/9781555816896.ch3
/content/10.1128/9781555816896.ch03.ch03eq06
Untitled
10.1128/9781555816896/e0056-05_thmb.gif
10.1128/9781555816896/e0056-05.gif
Untitled
Citation:
Maxfield P, Evershed R.
2011.
Phospholipid Fatty Acid Stable Isotope Probing Techniques in Microbial Ecology,
p 37-71.
In
Murrell J, Whiteley A (ed),
Stable Isotope Probing and Related Technologies.
ASM Press, Washington, DC.
doi: 10.1128/9781555816896.ch3
/content/10.1128/9781555816896.ch03.ch03fig14
FIGURE 14.
Concentrations of the total extracted PLFAs and comparison of the 13C-label concentration incorporated into each PLFA as nanograms of 13C per gram of soil dry weight following 17 to 18 weeks incubation under 2 ppmv 13CH4 for Bronydd Mawr NCaPK, CaPK, and Nil-graze soils. Error bars represent ±1 standard deviation (Maxfield et al., 2008a).
10.1128/9781555816896/f0057-01_thmb.gif
10.1128/9781555816896/f0057-01.gif
FIGURE 14.
Concentrations of the total extracted PLFAs and comparison of the 13C-label concentration incorporated into each PLFA as nanograms of 13C per gram of soil dry weight following 17 to 18 weeks incubation under 2 ppmv 13CH4 for Bronydd Mawr NCaPK, CaPK, and Nil-graze soils. Error bars represent ±1 standard deviation (Maxfield et al., 2008a).
Citation:
Maxfield P, Evershed R.
2011.
Phospholipid Fatty Acid Stable Isotope Probing Techniques in Microbial Ecology,
p 37-71.
In
Murrell J, Whiteley A (ed),
Stable Isotope Probing and Related Technologies.
ASM Press, Washington, DC.
doi: 10.1128/9781555816896.ch3
/content/10.1128/9781555816896.ch03.ch03fig15
FIGURE 15.
Comparison of Tenerife Andisol 13C-labeled PLFA distributions with published PLFA compositions of pure cultures of methanotrophic bacteria (
Bowman et al., 1993
;
Dedysh, 2002
;
Bodelier et al., 2009
). The PLFA compositions employed were the mole percentages of the PLFAs of pure cultures and the labeled PLFAs extracted from the soils. A hierarchical tree was produced by cluster analysis performed with the R v2.8.1 statistical package. Bootstrap probabilities were based on 1,000 repetitions (
Maxfield et al., 2009
).
10.1128/9781555816896/f0064-01_thmb.gif
10.1128/9781555816896/f0064-01.gif
FIGURE 15.
Comparison of Tenerife Andisol 13C-labeled PLFA distributions with published PLFA compositions of pure cultures of methanotrophic bacteria ( Bowman et al., 1993 ; Dedysh, 2002 ; Bodelier et al., 2009 ). The PLFA compositions employed were the mole percentages of the PLFAs of pure cultures and the labeled PLFAs extracted from the soils. A hierarchical tree was produced by cluster analysis performed with the R v2.8.1 statistical package. Bootstrap probabilities were based on 1,000 repetitions ( Maxfield et al., 2009 ).
Citation:
Maxfield P, Evershed R.
2011.
Phospholipid Fatty Acid Stable Isotope Probing Techniques in Microbial Ecology,
p 37-71.
In
Murrell J, Whiteley A (ed),
Stable Isotope Probing and Related Technologies.
ASM Press, Washington, DC.
doi: 10.1128/9781555816896.ch3
/content/10.1128/9781555816896.ch03.ch03fig16
FIGURE 16.
Landfill soil PLFA δ13C values following up to 27 days of incubation under 5,000 ppmv 13CH4 (1% enriched 13C) indicating primary (a), secondary (b), and tertiary (c) C assimilation (
Dildar, 2010
).
10.1128/9781555816896/f0066-01_thmb.gif
10.1128/9781555816896/f0066-01.gif
FIGURE 16.
Landfill soil PLFA δ13C values following up to 27 days of incubation under 5,000 ppmv 13CH4 (1% enriched 13C) indicating primary (a), secondary (b), and tertiary (c) C assimilation ( Dildar, 2010 ).
Citation:
Maxfield P, Evershed R.
2011.
Phospholipid Fatty Acid Stable Isotope Probing Techniques in Microbial Ecology,
p 37-71.
In
Murrell J, Whiteley A (ed),
Stable Isotope Probing and Related Technologies.
ASM Press, Washington, DC.
doi: 10.1128/9781555816896.ch3
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Tables
Phospholipid Fatty Acid Stable Isotope Probing Techniques in Microbial Ecology
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Citation:
Maxfield P, Evershed R.
2011.
Phospholipid Fatty Acid Stable Isotope Probing Techniques in Microbial Ecology,
p 37-71.
In
Murrell J, Whiteley A (ed),
Stable Isotope Probing and Related Technologies.
ASM Press, Washington, DC.
doi: 10.1128/9781555816896.ch3
/content/10.1128/9781555816896.ch03.ch03tab01
TABLE 1
The structure and distribution of major phospholipids
a
TABLE 1
The structure and distribution of major phospholipids a
Citation:
Maxfield P, Evershed R.
2011.
Phospholipid Fatty Acid Stable Isotope Probing Techniques in Microbial Ecology,
p 37-71.
In
Murrell J, Whiteley A (ed),
Stable Isotope Probing and Related Technologies.
ASM Press, Washington, DC.
doi: 10.1128/9781555816896.ch3
/content/10.1128/9781555816896.ch03.ch03tab02
TABLE 2
Nomenclature of some naturally occurring fatty acids
TABLE 2
Nomenclature of some naturally occurring fatty acids
Citation:
Maxfield P, Evershed R.
2011.
Phospholipid Fatty Acid Stable Isotope Probing Techniques in Microbial Ecology,
p 37-71.
In
Murrell J, Whiteley A (ed),
Stable Isotope Probing and Related Technologies.
ASM Press, Washington, DC.
doi: 10.1128/9781555816896.ch3
/content/10.1128/9781555816896.ch03.ch03tab03
TABLE 3
Classes of PLFAs associated with particular taxonomic or functional groups of microorganisms
a
TABLE 3
Classes of PLFAs associated with particular taxonomic or functional groups of microorganisms a
Citation:
Maxfield P, Evershed R.
2011.
Phospholipid Fatty Acid Stable Isotope Probing Techniques in Microbial Ecology,
p 37-71.
In
Murrell J, Whiteley A (ed),
Stable Isotope Probing and Related Technologies.
ASM Press, Washington, DC.
doi: 10.1128/9781555816896.ch3
/content/10.1128/9781555816896.ch03.ch03tab04
TABLE 4
Summary of PLFA 13C-labeling studies of environmental microbial communities
TABLE 4
Summary of PLFA 13C-labeling studies of environmental microbial communities
Citation:
Maxfield P, Evershed R.
2011.
Phospholipid Fatty Acid Stable Isotope Probing Techniques in Microbial Ecology,
p 37-71.
In
Murrell J, Whiteley A (ed),
Stable Isotope Probing and Related Technologies.
ASM Press, Washington, DC.
doi: 10.1128/9781555816896.ch3