Vibrio parahaemolyticus

Kaori Izutsu; Tetsuya Iida

doi:10.1128/9781555816902.ch6

Chapter 6 : Vibrio parahaemolyticus

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Affiliations: 1: Laboratory of Genomic Research on Pathogenic Bacteria, International Research Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan; 2: Laboratory of Genomic Research on Pathogenic Bacteria, International Research Center for Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan

Content Type: Monograph

Publication Year: 2011

Category: Applied and Industrial Microbiology; Food Microbiology

Book DOI: 10.1128/9781555816902

Chapter DOI: 10.1128/9781555816902.ch6

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Vibrio parahaemolyticus, Page 1 of 2

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Abstract:

This chapter talks about Vibrio parahaemolyticus that can cause fatal septicemia in immune-compromised hosts. The Kanagawa phenomenon (KP) has long been recognized as an effective marker for discriminating pathogenic from nonpathogenic V. parahaemolyticus strains and is used extensively as a clinical indicator for the virulence of this bacterium. Thermostable direct hemolysin (TDH) is considered an important virulence factor for V. parahaemolyticus. Genes classified in transcriptional regulation have specific roles in response to environmental changes. The most notable finding of the genome sequencing of V. parahaemolyticus was the presence in the genome of two sets of genes for the type III secretion system (T3SS). T3SS2 may also be useful for novel preventive and therapeutic methods, for example, as a target for vaccines or drugs against V. parahaemolyticus infections. The novel preventive and therapeutic methods discussed here are highly selective for this pathogen and may therefore contribute to therapies that are distinct from conventional treatments based on the administration of antibiotics. Studies on V. parahaemolyticus have mainly focused on the role of virulence factors TDH and thermostable direct related hemolysin (TRH), but genome sequencing of this bacterium has shed light on novel aspects of this organism. A notable finding in this context is the discovery of functional T3SS genes in the genome of V. parahaemolyticus, which are not found in the genomes of cholera-toxin-producing V. cholerae and V. parahaemolyticus.

Citation: Izutsu K, Iida T. 2011. Vibrio parahaemolyticus, p 77-84. In Fratamico P, Liu Y, Kathariou S (ed), Genomes of Foodborne and Waterborne Pathogens. ASM Press, Washington, DC. doi: 10.1128/9781555816902.ch6

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Mobile Genetic Elements: 0.429683
Type III Secretion System: 0.42738754
Type VI Secretion System: 0.41416934

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Vibrio parahaemolyticus

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Figure 1

Schematic view of bacterial T3SS apparatus. Effector proteins (black and shaded circles) are translocated directly into the cytosol of target eukaryotic cells.

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Figure 1

Schematic view of bacterial T3SS apparatus. Effector proteins (black and shaded circles) are translocated directly into the cytosol of target eukaryotic cells.

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Figure 2

Schematic representation of the hypothetical evolutionary acquisition of a T3SS-related gene cluster in V. parahaemolyticus and V. cholerae. The shaded ellipses show the T3SS-related gene clusters. Bold lines represent the evolutionary process. Circles indicate the strains of V. parahaemolyticus and V. cholerae. Shaded circles indicate that the strains possess T3SSalpha or T3SSbeta. The broken lines indicate that the T3SS-related gene clusters or cholera toxin has been acquired by horizontal gene transfer while the organisms were evolving. Cited from reference 32 .

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Figure 2

References

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1. Ansaruzzaman, M.,, M. Lucas,, J. L. Deen,, N. A. Bhuiyan,, X. Y. Wang,, A. Safa,, M. Sultana,, A. Chowdhury,, G. B. Nair,, D. A. Sack,, L. von Seidlein,, M. K. Puri,, M. Ali,, C. L. Chaignat,, J. D. Clemens, and, A. Barreto. 2005. Pandemic serovars (O3:K6 and O4:K68) of Vibrio parahaemolyticus associated with diarrhea in Mozambique: spread of the pandemic into the African continent. J. Clin. Microbiol. 43: 2559– 2562.

2. Bej, A. K.,, D. P. Patterson,, C. W. Brasher,, M. C. Vickery,, D. D. Jones, and, C. A. Kaysner. 1999. Detection of total and hemolysin-producing Vibrio parahaemolyticus in shellfish using multiplex PCR amplification of tlh, tdh and trh. J. Microbiol. Methods 36: 215– 225.

3. Blake, P. A.,, R. E. Weaver, and, D. G. Hollis. 1980. Diseases of humans (other than cholera) caused by vibrios. Annu. Rev. Microbiol. 34: 341– 367.

4. Boyd, E. F.,, A. L. Cohen,, L. M. Naughton,, D. W. Ussery,, T. T. Binnewies,, O. C. Stine, and, M. A. Parent. 2008. Molecular analysis of the emergence of pandemic Vibrio parahaemolyticus. BMC Microbiol. 8: 110.

5. Brasher, C. W.,, A. DePaola,, D. D. Jones, and, A. K. Bej. 1998. Detection of microbial pathogens in shellfish with multiplex PCR. Curr. Microbiol. 37: 101– 107.

6. Cabrera-Garcia, M. E.,, C. Vazquez-Salinas, and, E. I. Quinones-Ramirez. 2004. Serologic and molecular characterization of Vibrio parahaemolyticus strains isolated from seawater and fish products of the Gulf of Mexico. Appl. Environ. Microbiol. 70: 6401– 6406.

7. Chen, C. Y.,, K. M. Wu,, Y. C. Chang,, C. H. Chang,, H. C. Tsai,, T. L. Liao,, Y. M. Liu,, H. J. Chen,, A. B. Shen,, J. C. Li,, T. L. Su,, C. P. Shao,, C. T. Lee,, L. I. Hor, and, S. F. Tsai. 2003. Comparative genome analysis of Vibrio vulnificus, a marine pathogen. Genome Res. 13: 2577– 2587.

8. Choi, S. W.,, C. H. Park,, T. M. Silva,, E. I. Zaenker, and, R. L. Guerrant. 1996. To culture or not to culture: fecal lactoferrin screening for inflammatory bacterial diarrhea. J. Clin. Micro-biol. 34: 928– 932.

9. Chowdhury, N. R.,, S. Chakraborty,, T. Ramamurthy,, M. Nishibuchi,, S. Yamasaki,, Y. Takeda, and, G. B. Nair. 2000. Molecular evidence of clonal Vibrio parahaemolyticus pandemic strains. Emerg. Infect. Dis. 6: 631– 636.

10. Chowdhury, N. R.,, O. C. Stine,, J. G. Morris, and, G. B. Nair. 2004. Assessment of evolution of pandemic Vibrio parahaemolyticus by multilocus sequence typing. J. Clin. Microbiol. 42: 1280– 1282.

11. Daniels, N. A.,, L. MacKinnon,, R. Bishop,, S. Altekruse,, B. Ray,, R. M. Hammond,, S. Thompson,, S. Wilson,, N. H. Bean,, P. M. Griffin, and, L. Slutsker. 2000. Vibrio parahaemolyticus infections in the United States, 1973-1998. J. Infect. Dis. 181: 1661– 1666.

12. DePaola, A.,, C. A. Kaysner,, J. Bowers, and, D. W. Cook. 2000. Environmental investigations of Vibrio parahaemolyticus in oysters after outbreaks in Washington, Texas, and New York (1997 and 1998). Appl. Environ. Microbiol. 66: 4649– 4654.

13. Dziejman, M.,, D. Serruto,, V. C. Tam,, D. Sturtevant,, P. Diraphat,, S. M. Faruque,, M. H. Rahman,, J. F. Heidelberg,, J. Decker,, L. Li,, K. T. Montgomery,, G. Grills,, R. Kucher-lapati, and, J. J. Mekalanos. 2005. Genomic characterization of non-O1, non-O139 Vibrio cholerae reveals genes for a type III secretion system. Proc. Natl. Acad. Sci. USA. 102: 3465– 3470.

14. Fabbri, A.,, L. Falzano,, C. Frank,, G. Donelli,, P. Matarrese,, F. Raimondi,, A. Fasano, and, C. Fiorentini. 1999. Vibrio parahaemolyticus thermostable direct hemolysin modulates cy-toskeletal organization and calcium homeostasis in intestinal cultured cells. Infect. Immun. 67: 1139– 1148.

15. Fujino, T.,, Y. Okuno,, D. Nakada,, A. Aoyama,, K. Fukai,, T. Mukai, and, T. Ueho. 1953. On the bacteriological examination of shirasu-food poisoning. Med. J. Osaka Univ. 4: 299– 304.

16. Gonzalez-Escalona, N.,, V. Cachicas,, C. Acevedo,, M. L. Rio-seco,, J. A. Vergara,, F. Cabello,, J. Romero, and, R. T. Espejo. 2005. Vibrio parahaemolyticus diarrhea, Chile, 1998 and 2004. Emerg. Infect. Dis. 11: 129– 131.

17. Grant, I. R., H. J. Ball, and, M. T. Rowe. 1998. Isolation of Mycobacterium paratuberculosis from milk by immunomagnetic separation. Appl. Environ. Microbiol. 64: 3153– 3158.

18. Han, H.,, H. C. Wong,, B. Kan,, Z. Guo,, X. Zeng,, S. Yin,, X. Liu,, R. Yang,, D. Zhou. 2008. Genome plasticity of Vibrio parahaemolyticus: microevolution of the ‘pandemic group.’ BMC Genomics 9: 570.

19. Heidelberg, J. F.,, J. A. Eisen,, W. C. Nelson,, R. A. Clayton,, M. L. Gwinn,, R. J. Dodson,, D. H. Haft,, E. K. Hickey,, J. D. Peterson,, L. Umayam,, S. R. Gill,, K. E. Nelson,, T. D. Read,, H. Tettelin,, D. Richardson,, M. D. Ermolaeva,, J. Vamathevan,, S. Bass,, H. Qin,, I. Dragoi,, P. Sellers,, L. McDonald,, T. Utterback,, R. D. Fleishmann,, W. C. Nierman,, O. White,, S. L. Salzberg,, H. O. Smith,, R. R. Colwell,, J. J. Mekalanos,, J. C. Venter, and, C. M. Fraser. 2000. DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae. Nature 406: 477– 483.

20. Honda, T.,, Y. Ni, and, T. Miwatani. 1988. Purification and characterization of a hemolysin produced by a clinical isolate of Kanagawa phenomenon-negative Vibrio parahaemolyticus and related to the thermostable direct hemolysin. Infect. Immun. 56: 961– 965.

21. Honda, T., and, T. Iida. 1993. The pathogenicity of Vibrio parahaemolyticus and the role of the thermostable direct haemolysin and related haemolysins. Rev. Med. Microbiol. 4: 106– 113.

22. Hueck, C. J. 1998. Type III protein secretion systems in bacterial pathogens of animals and plants. Microbiol. Mol. Biol. Rev. 62: 379– 433.

23. Iguchi, T.,, S. Kondo, and, K. Hisatsune. 1995. Vibrio parahaemolyticus O serotypes from O1 to o13 all produce R-type lipopolysaccharide: SDS-PAGE and compositional sugar analysis. FEMS Microbiol. Lett. 130: 287– 292.

24. Izutsu, K.,, K. Kurokawa,, K. Tashiro,, S. Kuhara,, T. Hayashi,, T. Honda, and, T. Iida. 2008. Comparative genomic analysis using microarray demonstrates a strong correlation between the presence of the 80-kilobase pathogenicity island and pathogenicity in Kanagawa phenomenon-positive Vibrio parahaemolyticus strains. Infect. Immun. 76: 1016– 1023.

25. Janda, J. M.,, C. Powers,, R. G. Bryant, and, S. L. Abbott. 1988. Current perspectives on the epidemiology and pathogenesis of clinically significant Vibrio spp. Clin. Microbiol. Rev. 1: 245– 267.

26. Joseph, S.W.,, R. R. Colwell, and, J. B. Kaper. 1982. Vibrio parahaemolyticus and related halophilic Vibrios. Crit. Rev. Microbiol. 10: 77– 124.

27. Khare, S.,, T. A. Ficht,, R. L. Santos,, J. Romano,, A. R. Ficht,, S. Zhang,, I. R. Grant,, M. Libal,, D. Hunter, and, L. G. Adams. 2004. Rapid and sensitive detection of Mycobacterium avium subsp. paratuberculosis in bovine milk and feces by a combination of immunomagnetic bead separation-conventional PCR and real-time PCR. J. Clin. Microbiol. 42: 1075– 1081.

28. Makino, K.,, K. Oshima,, K. Kurokawa,, K. Yokoyama,, T. Uda,, K. Tagomori,, Y. Iijima,, M. Najima,, M. Nakano,, A. Ya-mashita,, Y. Kubota,, S. Kimura,, T. Yasunaga,, T. Honda,, H. Shinagawa,, M. Hattori, and, T. Iida. 2003. Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism distinct from that of V. cholerae. Lancet 361: 743– 749.

29. Martinez-Urtaza, J.,, A. Lozano-Leon,, A. DePaola,, M. Ishiba-shi,, K. Shimada,, M. Nishibuchi, and, E. Liebana. 2004. Characterization of pathogenic Vibrio parahaemolyticus isolates from clinical sources in Spain and comparison with Asian and North American pandemic isolates. J. Clin. Microbiol. 42: 4672– 4678.

30. Matsumoto, C.,, J. Okuda,, M. Ishibashi,, M. Iwanaga,, P. Garg,, T. Rammamurthy,, H. C. Wong,, A. Depaola,, Y. B. Kim,, M. J. Albert, and, M. Nishibuchi. 2000. Pandemic spread of an 0 3:K6 clone of Vibrio parahaemolyticus and emergence of related strains evidenced by arbitrarily primed PCR and toxRS sequence analyses. J. Clin. Microbiol. 38: 578– 585.

31. Naim, R.,, I. Yanagihara,, T. Iida, and, T. Honda. 2001. Vibrio parahaemolyticus thermostable direct hemolysin can induce an apoptotic cell death in Rat-1 cells from inside and outside of the cells. FEMS Microbiol. Lett. 195: 237– 244.

32. Nair, G. B.,, T. Ramamurthy,, S. K. Bhattacharya,, B. Dutta,, Y. Takeda, and, D. A. Sack. 2007. Global dissemination of Vibrio parahaemolyticus serotype 0 3:K6 and its serovariants. Clin. Microbiol. Rev. 20: 39– 48.

33. Nishibuchi, M., and, J. B. Kaper. 1995. Thermostable direct hemolysin gene of Vibrio parahaemolyticus: a virulence gene acquired by a marine bacterium. Infect. Immun. 63: 2093– 2099.

34. Okada, K.,, T. Iida,, K. Kita-Tsukamoto, and, T. Honda. 2005. Vibrios commonly possess two chromosomes. J. Bacteriol. 187: 752– 757.

35. Okada, N.,, T. Iida,, K. S. Park,, N. Goto,, T. Yasunaga,, H. Hiyoshi,, S. Matsuda,, T. Kodama, and, T. Honda. 2009. Identification and characterization of a novel type III secretion system in trh-positive Vibrio parahaemolyticus strain TH3996 reveal genetic lineage and diversity of pathogenic machinery beyond the species level. Infect. Immun. 77: 904– 913.

36. Okuda, J.,, M. Ishibashi,, E. Hayakawa,, T. Nishino,, Y. Takeda,, A. K. Mukhopadhyay,, S. Garg,, S. K. Bhattacharya,, G. B. Nair, and, M. Nishibuchi. 1997. Emergence of a unique O3:K6 clone of Vibrio parahaemolyticus in Calcutta, India, and isolation of strains from the same clonal group from Southeast Asian travelers arriving in Japan. J. Clin. Microbiol. 35: 3150– 3155.

37. Okura, M.,, R. Osawa,, E. Arakawa,, J. Terajima, and, H. Wa-tanabe. 2005. Identification of Vibrio parahaemolyticus pandemic group-specific DNA sequence by genomic subtraction. J Clin Microbiol. 43: 3533– 3536.

38. Olsvik, O.,, T. Popovic,, E. Skjerve,, K. S. Cudjoe,, E. Hornes,, J. Ugelstad, and, M. Uhlen. 1994. Magnetic separation techniques in diagnostic microbiology. Clin. Microbiol. Rev. 7: 43– 54.

39. Park, K. S.,, T. Iida,, Y. Yamaichi,, T. Oyagi,, K. Yamamoto, and, T. Honda. 2000. Genetic characterization of DNA region containing the trh and ure genes of Vibrio parahaemolyticus. Infect. Immun. 68: 5742– 5748.

40. Park, K. S.,, T. Ono,, M. Rokuda,, M. H. Jang,, K. Okada,, T. Iida, and, T. Honda. 2004. Functional characterization of two type III secretion systems of Vibrio parahaemolyticus. Infect. Immun. 72: 6659– 6665.

41. Park, K. S.,, T. Ono,, M. Rokuda,, M. H. Jang,, T. Iida, and, T. Honda. 2004. Cytotoxicity and enterotoxicity of the thermostable direct hemolysin-deletion mutants of Vibrio parahaemolyticus. Microbiol. Immunol. 48: 313– 318.

42. Raimondi, F.,, J. P. Kao,, C. Fiorentini,, A. Fabbri,, G. Donelli,, N. Gasparini,, A. Rubino, and, A. Fasano. 2000. Enterotoxicity and cytotoxicity of Vibrio parahaemolyticus thermostable direct hemolysin in vitro systems. Infect. Immun. 68: 3180– 3185.

43. Ruby, E. G.,, M. Urbanowski,, J. Campbell,, A. Dunn,, M. Faini,, R. Gunsalus,, P. Lostroh,, C. Lupp,, J. McCann,, D. Millikan,, A. Schaefer,, E. Stabb,, A. Stevens,, K. Visick,, C. Whistler, and, E. P. Greenberg. 2005. Complete genome sequence of Vibrio fis-cheri: a symbiotic bacterium with pathogenic congeners. Proc. Natl. Acad. Sci. USA 102: 3004– 3009.

44. Sakazaki, R.,, K. Tamura,, T. Kato,, Y. Obara,, S. Yamai, and, K. Hobo. 1968. Studies of the enteropathogenic, facultatively halophilic bacteria, Vibrio parahaemolyticus. III. Entero-pathogenicity. Jpn. J. Med. Sci. Biol. 21: 325– 331.

45. Takahashi, A.,, T. Iida,, R. Naim,, Y. Naykaya, and, T. Honda. 2001. Chloride secretion induced by thermostable direct hae-molysin of Vibrio parahaemolyticus depends on colonic cell maturation. J. Med. Microbiol. 50: 870– 878.

46. Tang, G. Q.,, T. Iida,, K. Yamamoto, and, T. Honda. 1995. Ca2+-independent cytotoxicity of Vibrio parahaemolyticus thermostable direct hemolysin (TDH) on Intestine 407, a cell line derived from human embryonic intestine. FEMS Microbiol. Lett. 134: 233– 238.

47. Tang, G.,, T. Iida,, H. Inoue,, M. Yutsudo,, K. Yamamoto, and, T. Honda. 1997. A mutant cell line resistant to Vibrio parahaemolyticus thermostable direct hemolysin (TDH): its potential in identification of putative receptor for TDH. Biochim. Bio-phys. Acta 1360: 277– 282.

48. Trucksis, M.,, J. Michalski,, Y. K. Deng,, J. B. Kaper. 1998. The Vibrio cholerae genome contains two unique circular chromosomes. Proc. Natl. Acad. Sci. USA 95: 14464- 14469.

49. Twedt, R. M., and, R. M. Novelli. 1971. Modified selective and differential isolation medium for Vibrio parahaemolyticus. Appl. Microbiol. 22: 593– 599.

50. Wong, H. C.,, S. H. Liu,, T. K. Wang,, C. L. Lee,, C. S. Chiou,, D. P. Liu,, M. Nishibuchi, and, B. K. Lee. 2000. Characteristics of Vibrio parahaemolyticus O3:K6 from Asia. Appl. Environ. Microbiol. 66: 3981– 3986.

51. Xu, M.,, K. Yamamoto, and, T. Honda. 1994. Construction and characterization of an isogenic mutant of Vibrio parahaemolyticus having a deletion in the thermostable direct hemolysin-related hemolysin gene (trh). J. Bacteriol. 176: 4757– 4760.

52. Yamaichi, Y.,, T. Iida,, K. S. Park,, K. Yamamoto, and, T. Honda. 1999. Physical and genetic map of the genome of Vibrio parahaemolyticus: presence of two chromosomes in Vibrio species. Mol. Microbiol. 31: 1513– 1521.