Chapter 3 : Chromogenic and Accelerated Cultural Methods

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Analysis of food samples by cultural and enrichment techniques remains an integral part of the examination of food for the presence or enumeration of foodborne pathogens. Enrichment methods have afforded the ability to detect as few as one cell per 500 g of food. In addition, enrichment techniques aid in the recovery of injured bacteria, which are often present in foods in a stressed condition because some foods may lack the optimal nutrients essential for bacterial growth, the food may present an environment not conducive to growth of the microorganisms, or the bacteria may be damaged during processing of the food. In this regard, the enrichment process provides an optimal growth medium and a period of time that can be effectively used by injured/stressed microorganisms to perform repair of cellular damage, allowing for resuscitation of the bacterial cells. Additionally, enrichment processes provide an opportunity for target bacterial cells to proliferate, while the growth of competing background microflora is truncated. This chapter describes many differential and selective media, which have been developed in response to the presence of bacterial pathogens capable of causing illness in humans, by employing unique phenotypic characteristics of these microorganisms to develop detection platforms based on cultural approaches. The selective media includes enterohemorrhagic (EHEC) media, media, media, media, media, and enumeration media. Several groups have investigated the use of a single broth to simultaneously enrich the concentrations of several bacterial species, followed by downstream detection.

Citation: Goodridge L, Bisha B. 2011. Chromogenic and Accelerated Cultural Methods, p 47-61. In Hoorfar J (ed), Rapid Detection, Characterization, and Enumeration of Foodborne Pathogens. ASM Press, Washington, DC. doi: 10.1128/9781555817121.ch3
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Examples of representative accelerated cultural methods for pre-enrichment/enrichment, plating, and confirmation of select foodborne pathogens

Citation: Goodridge L, Bisha B. 2011. Chromogenic and Accelerated Cultural Methods, p 47-61. In Hoorfar J (ed), Rapid Detection, Characterization, and Enumeration of Foodborne Pathogens. ASM Press, Washington, DC. doi: 10.1128/9781555817121.ch3

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