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Chapter 13 : in Pork, Beef, Poultry, and Egg

Authors: B. Malorny1, A. Bhunia2, H. J. M. Aarts3, C. Löfström4, J. Hoorfar5
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Affiliations: 1: Federal Institute for Risk Assessment, National Reference Laboratory for Salmonella, Diedersdorfer Weg 1, D-12277 Berlin, Germany; 2: Department of Food Science, Purdue University, 745 Agriculture Mall Dr., West Lafayette, IN 47907-1160; 3: Laboratory for Zoonoses and Environmental Microbiology (LZO), National Institute for Public Health (RIVM)—Centre for Infectious Disease Control, P.O. Box 1, 3720 BA, Bilthoven, The Netherlands; 4: National Food Institute, Technical University of Denmark, Mørkhøj Bygade 19, DK-2860 Søborg, Denmark; 5: National Food Institute, Technical University of Denmark, Mørkhøj Bygade 19, DK-2860 Søborg, Denmark
Content Type: Monograph
Publication Year: 2011

Category: Applied and Industrial Microbiology; Food Microbiology

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Abstract:

The contribution of the various foods and their categories to the occurrence of food-borne cases of human salmonellosis varies between countries depending on the prevalence of different serovars in food animals and in their various food production chains, as well as consumption habits and food preparation practices. Most contaminated eggs have on the shell surface only, and therefore, eggs are usually sanitized by a variety of methods and agents. Meat is another important source of food-borne salmonellosis, with poultry and pork implicated more often than beef and lamb. Methods to sequester target pathogenic bacteria from interfering food components and to concentrate them in small volumes are needed to enable the efficient application of rapid detection and identification methods. For rapid detection of in food including meat and eggs, three basic analytical principles are applied in practice: modified traditional culture methods, immunological methods, and nucleic acid-based methods.

Citation: Malorny B, Bhunia A, Aarts H, Löfström C, Hoorfar J. 2011. in Pork, Beef, Poultry, and Egg, p 179-194. In Hoorfar J (ed), Rapid Detection, Characterization, and Enumeration of Foodborne Pathogens. ASM Press, Washington, DC. doi: 10.1128/9781555817121.ch13
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Salmonella in Pork, Beef, Poultry, and Egg
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Citation: Malorny B, Bhunia A, Aarts H, Löfström C, Hoorfar J. 2011. in Pork, Beef, Poultry, and Egg, p 179-194. In Hoorfar J (ed), Rapid Detection, Characterization, and Enumeration of Foodborne Pathogens. ASM Press, Washington, DC. doi: 10.1128/9781555817121.ch13
/content/10.1128/9781555817121.ch13.ch13fig01
FIGURE 1
Comparison of quantitative real-time PCR and mini-MPN technique. Steps of procedures are displayed for real-time PCR (right) and mini-MPN (left).
10.1128/9781555817121/f0188-01_thmb.gif
10.1128/9781555817121/f0188-01.gif
Image of FIGURE 1
FIGURE 1

Comparison of quantitative real-time PCR and mini-MPN technique. Steps of procedures are displayed for real-time PCR (right) and mini-MPN (left).

Citation: Malorny B, Bhunia A, Aarts H, Löfström C, Hoorfar J. 2011. in Pork, Beef, Poultry, and Egg, p 179-194. In Hoorfar J (ed), Rapid Detection, Characterization, and Enumeration of Foodborne Pathogens. ASM Press, Washington, DC. doi: 10.1128/9781555817121.ch13
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Tables

Salmonella in Pork, Beef, Poultry, and Egg
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Citation: Malorny B, Bhunia A, Aarts H, Löfström C, Hoorfar J. 2011. in Pork, Beef, Poultry, and Egg, p 179-194. In Hoorfar J (ed), Rapid Detection, Characterization, and Enumeration of Foodborne Pathogens. ASM Press, Washington, DC. doi: 10.1128/9781555817121.ch13
/content/10.1128/9781555817121.ch13.ch13tab01
TABLE 1
Prevalence of in table eggs, poultry meat, and pork
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TABLE 1

Prevalence of in table eggs, poultry meat, and pork

Citation: Malorny B, Bhunia A, Aarts H, Löfström C, Hoorfar J. 2011. in Pork, Beef, Poultry, and Egg, p 179-194. In Hoorfar J (ed), Rapid Detection, Characterization, and Enumeration of Foodborne Pathogens. ASM Press, Washington, DC. doi: 10.1128/9781555817121.ch13
/content/10.1128/9781555817121.ch13.ch13tab02
TABLE 2
PCR reagents and cycle conditions for blockcyclers of typical real-time PCR
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TABLE 2

PCR reagents and cycle conditions for blockcyclers of typical real-time PCR

Citation: Malorny B, Bhunia A, Aarts H, Löfström C, Hoorfar J. 2011. in Pork, Beef, Poultry, and Egg, p 179-194. In Hoorfar J (ed), Rapid Detection, Characterization, and Enumeration of Foodborne Pathogens. ASM Press, Washington, DC. doi: 10.1128/9781555817121.ch13
/content/10.1128/9781555817121.ch13.ch13tab03
TABLE 3
Reagents, cycling conditions, and fragment analyzer run parameters used in the MLVA-PCR for serovar Typhimurium or serovar Enteritidis
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TABLE 3

Reagents, cycling conditions, and fragment analyzer run parameters used in the MLVA-PCR for serovar Typhimurium or serovar Enteritidis

Citation: Malorny B, Bhunia A, Aarts H, Löfström C, Hoorfar J. 2011. in Pork, Beef, Poultry, and Egg, p 179-194. In Hoorfar J (ed), Rapid Detection, Characterization, and Enumeration of Foodborne Pathogens. ASM Press, Washington, DC. doi: 10.1128/9781555817121.ch13

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