Chapter 19 : Biofilm Formation in

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This chapter focuses on biofilm formation by the pathogenic fungus . Biofilms are surface-associated microbial communities surrounded by an extracellular matrix. The chapter examines the steps of biofilm formation, from the genes known to function in biofilm development, the cell-cell communication within the biofilm, the environmental responses that contribute to biofilm formation, the drug resistance of biofilms, and experimental techniques used to study biofilms. Cell wall genes and adhesins provide mechanisms that promote biofilm initiation, and the transcription factors that regulate their expression couple biofilm initiation with internal and external signals. The study of transcription factors has laid the framework for gene regulatory networks. While much is now known, detailed testing of other known adherence factors, cell wall proteins, transcription factors, kinases, and others is required to identify more genes involved in biofilm formation, and the elucidation of upstream regulation of these factors will allow for greater insights into the signaling events important for biofilm development. Quorum sensing governs functions as diverse as bioluminescence and virulence. It has a vital role in bacterial biofilm dynamics, and its role in biofilms is now beginning to be understood. While major challenges lie ahead to further refine the gene regulatory networks and correlate them to in vivo results, it is nonetheless an exciting time for the field of biofilm formation.

Citation: Finkel J, Mitchell A. 2012. Biofilm Formation in , p 299-315. In Calderone R, Clancy C (ed), and Candidiasis, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817176.ch19
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Image of FIGURE 1

The three different morphologies of cells. (A) Yeast form cells comprise either a single oval cell or an oval mother cell and smaller daughter cells (seen attached to most cells). (B) Pseudohyphal cells, abnormal elongated budding cells, can contain multiple buds. (C) Hyphal cells are large oval cells with long narrow continuous germ tube protrusions. doi:10.1128/9781555817176.ch19.f1

Citation: Finkel J, Mitchell A. 2012. Biofilm Formation in , p 299-315. In Calderone R, Clancy C (ed), and Candidiasis, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817176.ch19
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Image of FIGURE 2

Schematic diagram of the different steps of in vitro biofilm development. (A) In the first step, yeast form cells adhere to the substrate. (B) In the second step, the cells multiply and accumulate. Germ tubes begin to form, and extracellular matrix production begins. (C) In the third step, the biofilm enlarges, with increased cell density, yeast cells, and hyphal cells. Extracellular matrix production increases, enveloping the biofilm. (D) In the final step, yeast cells are dispersed to further colonize the surrounding environment. doi:10.1128/9781555817176.ch19.f2

Citation: Finkel J, Mitchell A. 2012. Biofilm Formation in , p 299-315. In Calderone R, Clancy C (ed), and Candidiasis, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817176.ch19
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Image of FIGURE 3

Graphic representation of known pathways involved in biofilm formation. The first three steps of biofilm formation are presented. Arrows with a plus sign represent positive genetic control. “T” bars with a minus sign represent inhibitory action by the protein(s) on the particular step. Those proteins not associated with a particular pathway are presented as follows in the category that best represents their hypothesized function: adhesion, Gin4, Ire1, and Och1; biomass, Als2, Pep12, Pmt1, and Ume6; filamentation, Cbk1, Efg1, Flo8, Kem1, Mds3, Mkc1, and Suv3; and rescue of biofilm defect, Als3, Als5, Als7, Als9, Czf1, and Ece1. doi:10.1128/9781555817176.ch19.f3

Citation: Finkel J, Mitchell A. 2012. Biofilm Formation in , p 299-315. In Calderone R, Clancy C (ed), and Candidiasis, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817176.ch19
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Genes that govern biofilm formation

Citation: Finkel J, Mitchell A. 2012. Biofilm Formation in , p 299-315. In Calderone R, Clancy C (ed), and Candidiasis, Second Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817176.ch19

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