Temporal Gene Regulation during the Chlamydial Developmental Cycle

Ming Tan

doi:10.1128/9781555817329.ch7

Chapter 7 : Temporal Gene Regulation during the Chlamydial Developmental Cycle

Author: Ming Tan¹

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Affiliations: 1: Departments of Microbiology & Molecular Genetics, and Medicine, University of California, Irvine, CA 92697-4025

Content Type: Monograph

Publication Year: 2012

Category: Bacterial Pathogenesis

Book DOI: 10.1128/9781555817329

Chapter DOI: 10.1128/9781555817329.ch7

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Abstract:

This chapter reviews the mechanisms of temporal gene regulation utilized during the three main stages of the developmental cycle, as well as how transcription is silenced in elementary body (EB). It begins with midcycle because the majority of chlamydial genes are regulated during this stage of reticulate body (RB) growth and replication. The transcriptional regulation of midcycle genes, late genes, EBs, and early genes in sequence, mirroring the events that occur during replication, conversion of an RB into an EB, and then back again into an RB at the start of a new round of infection are discussed. Alternative sigma factors have important roles in the regulation of virulence gene expression in many bacteria. A study showed that a subset of early genes resemble midcycle genes in having promoters that are supercoiling responsive. There is emerging evidence that gene expression during the chlamydial developmental cycle is also regulated by small RNAs (sRNAs). Two complementary genome-wide studies have since identified many more sRNAs in C. trachomatis. Chlamydia spp. are likely to encode more sRNAs because neither study examined chlamydial RNA before 24 hpi, and both would therefore have missed sRNAs that are only present at earlier times.

Citation: Tan M. 2012. Temporal Gene Regulation during the Chlamydial Developmental Cycle, p 149-169. In Tan M, Bavoil P (ed), Intracellular Pathogens I: Chlamydiales. ASM Press, Washington, DC. doi: 10.1128/9781555817329.ch7

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Temporal Gene Regulation during the Chlamydial Developmental Cycle

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FIGURE 1

Proposed mechanisms for temporal regulation of gene expression during the chlamydial developmental cycle. doi:10.1128/9781555817329.ch7.f1

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FIGURE 1

Proposed mechanisms for temporal regulation of gene expression during the chlamydial developmental cycle. doi:10.1128/9781555817329.ch7.f1

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Tables

Temporal Gene Regulation during the Chlamydial Developmental Cycle

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TABLE 1

Chlamydial factors for which there are data to support a role in regulating temporal gene expression during the developmental cycle

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10.1128/9781555817329/Chapter7_Table1.gif

TABLE 1

Chlamydial factors for which there are data to support a role in regulating temporal gene expression during the developmental cycle