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Chapter 5 : Automation and Design of the Clinical Microbiology Laboratory

Authors: Christopher D. Doern, Martin Holfelder
Content Type: Reference
Publication Year: 2015

Category: Clinical Microbiology

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Abstract:

This chapter discusses laboratory design and the growing impact of laboratory automation. The chapter is divided into two sections. The first section addresses more traditional considerations of laboratory design such as staffing, workflow, laboratory location, and the role of efficiency programs such as lean and Six Sigma. The second section discusses currently available systems for automating the clinical microbiology laboratory. Individual technologies for specimen processing, organism identification, and susceptibility testing as well as total lab automation are covered. Important considerations for purchase and utilization are outlined to aid readers in deciding which technology is right for their lab and how best to approach implementation.

Citation: Doern C, Holfelder M. 2015. Automation and Design of the Clinical Microbiology Laboratory, p 44-53. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch5
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Automation and Design of the Clinical Microbiology Laboratory
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Citation: Doern C, Holfelder M. 2015. Automation and Design of the Clinical Microbiology Laboratory, p 44-53. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch5
/content/10.1128/9781555817381.mcm11.ch05.ch05.fig001
FIGURE 1

InoqulA magnetic bead. doi:10.1128/9781555817381.ch5.f1

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Image of FIGURE 1
FIGURE 1

InoqulA magnetic bead. doi:10.1128/9781555817381.ch5.f1

Citation: Doern C, Holfelder M. 2015. Automation and Design of the Clinical Microbiology Laboratory, p 44-53. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch5
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FIGURE 2

PREVI Isola-inoculated agar plate. doi:10.1128/9781555817381.ch5.f2

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Image of FIGURE 2
FIGURE 2

PREVI Isola-inoculated agar plate. doi:10.1128/9781555817381.ch5.f2

Citation: Doern C, Holfelder M. 2015. Automation and Design of the Clinical Microbiology Laboratory, p 44-53. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch5
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FIGURE 3

BD-Kiestra TLA with barcoding, inoculation (InoqulA) (depth, 93.5 cm; width, 417.2 cm; weight, 770 kg), and incubation (ReadA) (depth, 164.0 cm; width, 87 cm; weight, 430–730 kg). doi:10.1128/9781555817381.ch5.f3

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10.1128/9781555817381/9781555817381.ch5.f3.gif
Image of FIGURE 3
FIGURE 3

BD-Kiestra TLA with barcoding, inoculation (InoqulA) (depth, 93.5 cm; width, 417.2 cm; weight, 770 kg), and incubation (ReadA) (depth, 164.0 cm; width, 87 cm; weight, 430–730 kg). doi:10.1128/9781555817381.ch5.f3

Citation: Doern C, Holfelder M. 2015. Automation and Design of the Clinical Microbiology Laboratory, p 44-53. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch5
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FIGURE 4

FMLA, bioMérieux, smart incubation unit (depth, 145.7 cm; width, 128.0 cm; weight, 850 to 950 kg); PREVI Isola (depth, 90.7 cm; width, 144.5 cm; weight, 298 kg). doi:10.1128/9781555817381.ch5.f4

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10.1128/9781555817381/9781555817381.ch5.f4.gif
Image of FIGURE 4
FIGURE 4

FMLA, bioMérieux, smart incubation unit (depth, 145.7 cm; width, 128.0 cm; weight, 850 to 950 kg); PREVI Isola (depth, 90.7 cm; width, 144.5 cm; weight, 298 kg). doi:10.1128/9781555817381.ch5.f4

Citation: Doern C, Holfelder M. 2015. Automation and Design of the Clinical Microbiology Laboratory, p 44-53. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch5
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FIGURE 5

WASPLab, Copan: WASP (depth, 119.1 cm; width, 194.9 cm; weight, 700 kg); double incubator (depth, 83.0 cm; width, 174.0 cm; weight, 700 kg); imaging system (depth, 121.3 cm; width, 37.5 cm; weight, 250 kg). doi:10.1128/9781555817381.ch5.f5

10.1128/9781555817381/9781555817381.ch5.f5_thmb.gif
10.1128/9781555817381/9781555817381.ch5.f5.gif
Image of FIGURE 5
FIGURE 5

WASPLab, Copan: WASP (depth, 119.1 cm; width, 194.9 cm; weight, 700 kg); double incubator (depth, 83.0 cm; width, 174.0 cm; weight, 700 kg); imaging system (depth, 121.3 cm; width, 37.5 cm; weight, 250 kg). doi:10.1128/9781555817381.ch5.f5

Citation: Doern C, Holfelder M. 2015. Automation and Design of the Clinical Microbiology Laboratory, p 44-53. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch5
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References

/content/book/10.1128/9781555817381.mcm11.ch05
1. US Department of Health and Human Services. 2009. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. Centers for Disease Control and Prevention, Atlanta, GA.
2. Baselski V,, Carey R,, Clarridge J,, Weissfeld A. 2005. Survey of Clinical Microbiology Laboratory Workloads, Productivity Rates and Staffing Vacancies. American Society for Microbiology, Washington, DC.
3. Jones JL,, Lopez A,, Wahlquist SP,, Nadle J,, Wilson M. 2004. Survey of clinical laboratory practices for parasitic diseases. Clin Infect Dis 38( Suppl 3): S198– S202.
4. Versalovic J,, Funke G,, Jorgensen J,, Landry ML,, Warnock D. 2011. Manual of Clinical Microbiology, 10th ed, vol 1. ASM Press, Washington, DC.
5. Campos J. 2012. Lean lab in action. MLO Med Lab Obs 44: 25– 29.
6. American Society of Clinical Pathologists. 1996. Board of Registry Study Guide. Clinical Laboratory Certification Examinations, 4th ed. American Society of Clinical Pathologists, Chicago, IL.
7. Wimmer JL,, Long SW,, Cernoch P,, Land GA,, Davis JR,, Musser JM,, Olsen RJ. 2012. Strategy for rapid identification and antibiotic susceptibility testing of gram-negative bacteria directly recovered from positive blood cultures using the Bruker MALDI Biotyper and the BD Phoenix system. J Clin Microbiol 50: 2452– 2454.
8. Tan KE,, Ellis BC,, Lee R,, Stamper PD,, Zhang SX,, Carroll KC. 2012. Prospective evaluation of a matrix-assisted laser desorption ionization–time of flight mass spectrometry system in a hospital clinical microbiology laboratory for identification of bacteria and yeasts: a bench-by-bench study for assessing the impact on time to identification and cost-effectiveness. J Clin Microbiol 50: 3301– 3308.
9. Patel R. 2013. Matrix-assisted laser desorption ionization–time of flight mass spectrometry in clinical microbiology. Clin Infect Dis 57: 564– 572.
10. Still M. 2013. MTRA, bitte melden. Dtsch Arzteblatt 110: 105– 106.
11. Mazzocato P,, Savage C,, Brommels M,, Aronsson H,, Thor J. 2010. Lean thinking in healthcare: a realist review of the literature. Qual Saf Health Care 19: 376– 382.
12. Riebling N,, Condon S,, Gopen D. 2004. Toward error free lab work. Six Sigma Forum Mag 4:6.
13. Rutledge J,, Xu M,, Simpson J. 2010. Application of the Toyota production system improves core laboratory operations. Am J Clin Pathol 133: 24– 31.
14. Carlson RO,, Amirahmadi F,, Hernandez JS. 2012. A primer on the cost of quality for improvement of laboratory and pathology specimen processes. Am J Clin Pathol 138: 347– 354.
15. Llopis MA,, Trujillo G,, Llovet MI,, Tarres E,, Ibarz M,, Biosca C,, Ruiz R,, Kirchner MJ,, Alvarez V,, Busquets G,, Domenech MV,, Figueres C,, Minchinela J,, Pastor RM,, Perich C,, Ricos C,, Sansalvador M,, Palmada MS. 2011. Quality indicators and specifications for key analytical-extranalytical processes in the clinical laboratory. Five years’ experience using the Six Sigma concept. Clin Chem Lab Med 49: 463– 470.
16. Vanker N,, van Wyk J,, Zemlin AE,, Erasmus RT. 2010. A Six Sigma approach to the rate and clinical effect of registration errors in a laboratory. J Clin Pathol 63: 434– 437.
17. Gras JM,, Philippe M. 2007. Application of the Six Sigma concept in clinical laboratories: a review. Clin Chem Lab Med 45: 789– 796.
18. Stankovic AK,, Romeo P. 2007. The role of in vitro diagnostic companies in reducing laboratory error. Clin Chem Lab Med 45: 781– 788.
19. Coskun A. 2007. Six Sigma and laboratory consultation. Clin Chem Lab Med 45: 121– 123.
20. Persoon TJ,, Zaleski S,, Frerichs J. 2006. Improving preanalytic processes using the principles of lean production (Toyota production system). Am J Clin Pathol 125: 16– 25.
21. Hawker CD. 2007. Laboratory automation: total and subtotal. Clin Lab Med 27: 749– 770, vi.
22. Tilton RC,, Ryan RW. 1978. Evaluation of an automated agar plate streaker. J Clin Microbiol 7: 298– 304.
23. Mischnik A,, Mieth M,, Busch CJ,, Hofer S,, Zimmermann S. 2012. First evaluation of automated specimen inoculation for wound swab samples by use of the Previ Isola system compared to manual inoculation in a routine laboratory: finding a cost-effective and accurate approach. J Clin Microbiol 50: 2732– 2736.
24. Bourbeau PP,, Swartz BL. 2009. First evaluation of the WASP, a new automated microbiology plating instrument. J Clin Microbiol 47: 1101– 1106.
25. Jones G,, Matthews R,, Cunningham R,, Jenks P. 2011. Comparison of automated processing of flocked swabs with manual processing of fiber swabs for detection of nasal carriage of Staphylococcus aureus. J Clin Microbiol 49: 2717– 2718.
26. Glasson JH,, Guthrie LH,, Nielsen DJ,, Bethell FA. 2008. Evaluation of an automated instrument for inoculating and spreading samples onto agar plates. J Clin Microbiol 46: 1281– 1284.
27. Mulatero F,, Bonnardel V,, Micolaud C. 2011. The way forward for fast microbiology. Clin Microbiol Infect 17: 661– 667.
28. Bourbeau PP,, Ledeboer NA. 2013. Automation in clinical microbiology. J Clin Microbiol 51: 1658– 1665.
29. Dumitrescu O,, Dauwalder O,, Lina G. 2011. Present and future automation in bacteriology. Clin Microbiol Infect 17: 649– 650.
30. Matthews S,, Deutekom J. 2011. The future of diagnostic bacteriology. Clin Microbiol Infect 17: 651– 654.
31. Van Horn KG,, Audette CD,, Sebeck D,, Tucker KA. 2008. Comparison of the Copan ESwab system with two Amies agar swab transport systems for maintenance of microorganism viability. J Clin Microbiol 46: 1655– 1658.
32. Fontana C,, Favaro M,, Limongi D,, Pivonkova J,, Favalli C. 2009. Comparison of the eSwab collection and transportation system to an Amies gel transystem for Gram stain of clinical specimens. BMC Res Notes 2:244.
33. Greub G,, Prod’ hom G. 2011. Automation in clinical bacteriology: what system to choose? Clin Microbiol Infect 17:655–660.
34. Garcia LS. 2010. Clinical Microbiology Procedures Handbook, 3rd ed. ASM Press, Washington, DC.

Tables

Automation and Design of the Clinical Microbiology Laboratory
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Citation: Doern C, Holfelder M. 2015. Automation and Design of the Clinical Microbiology Laboratory, p 44-53. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch5
/content/10.1128/9781555817381.mcm11.ch05.ch05.tab001
TABLE 1

Automated inoculation systems

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TABLE 1

Automated inoculation systems

Citation: Doern C, Holfelder M. 2015. Automation and Design of the Clinical Microbiology Laboratory, p 44-53. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch5

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