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Chapter 114 : Specimen Collection, Transport, and Processing: Mycology

Author: Karin L. McGowan
Content Type: Reference
Publication Year: 2015

Category: Clinical Microbiology

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Abstract:

Successful laboratory diagnosis of fungal infections requires attentiveness on the part of physicians and nurses, proper collection and transport of appropriate specimens, and comprehensive procedures in the laboratory. This chapter offers guidelines for specimen collection and transport, specimen handling, specimen pretreatment and processing in the laboratory, medium selection, and incubation of cultures.

Citation: McGowan K. 2015. Specimen Collection, Transport, and Processing: Mycology, p 1944-1954. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch114
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Specimen Collection, Transport, and Processing: Mycology
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Citation: McGowan K. 2015. Specimen Collection, Transport, and Processing: Mycology, p 1944-1954. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch114
/content/10.1128/9781555817381.mcm11.ch114.ch114.fig001
FIGURE 1

Growth of a dermatophyte () after 4 days of aerobic incubation at 30°C following toothbrush inoculation of Mycosel agar. Courtesy of Marilyn A. Leet, Children’s Hospital of Philadelphia, Philadelphia, PA. doi:10.1128/9781555817381.ch114.f1

10.1128/9781555817381/9781555817381.ch114.f1_thmb.gif
10.1128/9781555817381/9781555817381.ch114.f1.gif
Image of FIGURE 1
FIGURE 1

Growth of a dermatophyte () after 4 days of aerobic incubation at 30°C following toothbrush inoculation of Mycosel agar. Courtesy of Marilyn A. Leet, Children’s Hospital of Philadelphia, Philadelphia, PA. doi:10.1128/9781555817381.ch114.f1

Citation: McGowan K. 2015. Specimen Collection, Transport, and Processing: Mycology, p 1944-1954. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch114
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FIGURE 2

Early growth of from tissue piece embedded in Sabouraud dextrose agar with gentamicin incubated aerobically at 30°C. Courtesy of Marilyn A. Leet, Children’s Hospital of Philadelphia, Philadelphia, PA. doi:10.1128/9781555817381.ch114.f2

10.1128/9781555817381/9781555817381.ch114.f2_thmb.gif
10.1128/9781555817381/9781555817381.ch114.f2.gif
Image of FIGURE 2
FIGURE 2

Early growth of from tissue piece embedded in Sabouraud dextrose agar with gentamicin incubated aerobically at 30°C. Courtesy of Marilyn A. Leet, Children’s Hospital of Philadelphia, Philadelphia, PA. doi:10.1128/9781555817381.ch114.f2

Citation: McGowan K. 2015. Specimen Collection, Transport, and Processing: Mycology, p 1944-1954. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch114
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Tables

Specimen Collection, Transport, and Processing: Mycology
[com.pub2web.rdf.cci.facet.ContentItem[id=http://asm.metastore.ingenta.com/content/author/10.1128/9781555817381.mcm11.ch114-1,webId=/content/author/10.1128/9781555817381.mcm11.ch114-1,properties={foaf_givenname=Karin L., foaf_name=Karin L. McGowan, foaf_surname=McGowan}]]
Citation: McGowan K. 2015. Specimen Collection, Transport, and Processing: Mycology, p 1944-1954. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch114
/content/10.1128/9781555817381.mcm11.ch114.ch114.tab001
TABLE 1

Specimen collection and transport guidelines

Generic image for table
TABLE 1

Specimen collection and transport guidelines

Citation: McGowan K. 2015. Specimen Collection, Transport, and Processing: Mycology, p 1944-1954. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch114
/content/10.1128/9781555817381.mcm11.ch114.ch114.tab002
TABLE 2

Common clinical sites for laboratory recovery of pathogenic fungi

Generic image for table
TABLE 2

Common clinical sites for laboratory recovery of pathogenic fungi

Citation: McGowan K. 2015. Specimen Collection, Transport, and Processing: Mycology, p 1944-1954. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch114
/content/10.1128/9781555817381.mcm11.ch114.ch114.tab003
TABLE 3

Pretreatment of clinical specimens prior to plating

Generic image for table
TABLE 3

Pretreatment of clinical specimens prior to plating

Citation: McGowan K. 2015. Specimen Collection, Transport, and Processing: Mycology, p 1944-1954. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch114
/content/10.1128/9781555817381.mcm11.ch114.ch114.tab004
TABLE 4

Media suggested for the recovery of fungi from clinical specimens

Generic image for table
TABLE 4

Media suggested for the recovery of fungi from clinical specimens

Citation: McGowan K. 2015. Specimen Collection, Transport, and Processing: Mycology, p 1944-1954. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch114

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