Chapter 27 : Listeria and Erysipelothrix

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and are Gram-positive, rod-shaped bacteria. They occur naturally in a variety of animal species and the environment. is the causative agent of listeriosis, which manifests mainly as septicemia or meningitis. People with an underlying condition, pregnant women, and individuals ≥60 years of age have a markedly increased risk of acquiring listeriosis. can enter the food chain in various manners, and since it is able to grow even at temperatures below 10°C, it is an important cause of food-related outbreaks. Detection of is facilitated by culture on selective media, followed by biochemical, molecular, and mass spectrometry identification methods. Typing methods, like pulsed-field gel electrophoresis and also faster molecular methods, are available for investigation of outbreaks and surveillance purposes. Listeriosis is primarily treated with an aminopenicillin, while is intrinsically resistant to cephalosporins. causes erysipeloid in humans, a zoonosis which is mostly acquired by exposure to contaminated fish and meat. Erysipeloid is characterized by localized cellulitis accompanied by local lymphadenopathy and sometimes systemic symptoms. can be differentiated from other Gram-positive rods by biochemical and molecular methods and is typically positive for HS. Typing of isolates is of limited value, since most cases are caused by two serogroups. Erysipeloid is primarily treated with penicillin or ampicillin, and of note, is intrinsically resistant to vancomycin. Up-to-date detection, identification, and typing methods for spp. and as well as antimicrobial susceptibilities are described in detail in this .

Citation: Wellinghausen N. 2015. Listeria and Erysipelothrix, p 462-473. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch27
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Image of FIGURE 1

Macroscopic view of colonies on 5% human blood agar plates after 24 h of incubation. (A) : discrete zone of beta-hemolysis under the removed colonies. (B) : no hemolysis. (C) : wide zone of beta-hemolysis around the colonies. doi:10.1128/9781555817381.ch27.f1

Citation: Wellinghausen N. 2015. Listeria and Erysipelothrix, p 462-473. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch27
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Image of FIGURE 2

CAMP test done with CIP 5710 (top plate) and CIP 5869 (bottom plate) after 24 h of incubation. Upper right, ; lower right, middle left, . doi:10.1128/9781555817381.ch27.f2

Citation: Wellinghausen N. 2015. Listeria and Erysipelothrix, p 462-473. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch27
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Biochemical differentiation of medically relevant and selected environmental species in the genus

Citation: Wellinghausen N. 2015. Listeria and Erysipelothrix, p 462-473. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch27

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