Chapter 89 : Rhinoviruses

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Rhinoviruses, Page 1 of 2

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Human rhinoviruses (HRVs) are members of the family Previously a separate genus, HRVs have been reclassified into three separate species (A, B, C) within the genus. HRV culture isolates were originally classified into 99 serotypes based on neutralization with type-specific antisera. The wider use of molecular methods has led to the discovery of a novel group of HRVs, classified as species “C.” HRV infections are widespread year-round, with peaks in autumn and late spring in temperate zones. HRVs cause about two-thirds of cases of the common cold and thus are responsible for more episodes of human illness than any other infectious agent. Recently, with the use of molecular methods, more severe consequences of HRV infections have been recognized. HRV has been detected in lower respiratory tract infections in patients of all ages hospitalized with wheezing or pneumonia and in patients with chronic illnesses, cancer, immunosuppressive illnesses, transplants, and underlying pulmonary disease such as asthma. While nucleic acid amplification assays have revolutionized HRV detection, the interpretation of a positive result can be problematic. HRV can be detected in asymptomatic persons, can be shed for weeks after respiratory symptoms resolve, are prone to cause serial infections, and can occur as coinfections with other viruses or bacteria. Thus, interpretation in the individual case often relies on risk factors and recovery of other pathogens. Further research is needed to determine the impact of HRV detection on patient management and develop effective therapies.

Citation: Landry M, Lu X. 2015. Rhinoviruses, p 1551-1564. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch89
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Image of FIGURE 1

HRV genome structure. The genome consists one single open reading frame flanked by 5′ and 3′ untranslated regions (UTR). A small viral protein, VPg, is covalently linked to the 5′ UTR. VP, viral protein; IRES, internal ribosome entry site; cre, -acting replication element. Reproduced with permission from . doi:10.1128/9781555817381.ch89.f1

Citation: Landry M, Lu X. 2015. Rhinoviruses, p 1551-1564. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch89
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Image of FIGURE 2

HRV CPE in human embryonic lung fibroblasts. (A) Uninfected cells; (B) early focus of rhinovirus CPE; (C) more advanced rhinovirus CPE. Magnification, ×100. doi:10.1128/9781555817381.ch89.f2

Citation: Landry M, Lu X. 2015. Rhinoviruses, p 1551-1564. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch89
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1. Knowles NJ,, Hovi T,, Hyypiä T,, King AMQ,, Lindberg AM,, Pallansch MA,, Palmenberg AC,, Simmonds P,, Skern T,, Stanway G,, Yamashita T,, Zell R. 2012. Picornaviridae. Elsevier, San Diego. CA.
2. Zoll J,, Erkens Hulshof S,, Lanke K,, Verduyn Lunel F,, Melchers WJG,, Schoondermark-van de Ven E,, Roivainen M,, Galama JMD,, van Kuppeveld FJM. 2009. Saffold virus, a human Theiler’s-like cardiovirus, is ubiquitous and causes infection early in life. PLoS Pathog 5: e1000416.
3. Ledford RM,, Patel NR,, Demenczuk TM,, Watanyar A,, Herbertz T,, Collett MS,, Pevear DC. 2004. VP1 sequencing of all human rhinovirus serotypes: insights into genus phylogeny and susceptibility to antiviral capsid-binding compounds. J Virol 78: 36633674.
4. Laine P,, Savolainen C,, Blomqvist S,, Hovi T. 2005. Phylogenetic analysis of human rhinovirus capsid protein VP1 and 2A protease coding sequences confirms shared genus-like relationships with human enteroviruses. J Gen Virol 86: 697706.
5. Savolainen C,, Blomqvist S,, Mulders MN,, Hovi T. 2002. Genetic clustering of all 102 human rhinovirus prototype strains: serotype 87 is close to human enterovirus 70. J Gen Virol 83: 333340.
6. Blomqvist S,, Savolainen C,, Raman L,, Roivainen M,, Hovi T. 2002. Human rhinovirus 87 and enterovirus 68 represent a unique serotype with rhinovirus and enterovirus features. J Clin Microbiol 40: 42184223.
7. Uncapher CR,, DeWitt CM,, Colonno RJ. 1991. The major and minor group receptor families contain all but one human rhinovirus serotype. Virology 180: 814817.
8. Vlasak M,, Roivainen M,, Reithmayer M,, Goesler I,, Laine P,, Snyers L,, Hovi T,, Blaas D. 2005. The minor receptor group of human rhinovirus (HRV) includes HRV23 and HRV25, but the presence of a lysine in the VP1 HI loop is not sufficient for receptor binding. J Virol 79: 73897395.
9. Rossmann MG,, Bella J,, Kolatkar PR,, He Y,, Wimmer E,, Kuhn RJ,, Baker TS. 2000. Cell recognition and entry by rhino- and enteroviruses. Virology 269: 239247.
10. Andries K,, Dewindt B,, Snoeks J,, Wouters L,, Moereels H,, Lewi PJ,, Janssen PA. 1990. Two groups of rhinoviruses revealed by a panel of antiviral compounds present sequence divergence and differential pathogenicity. J Virol 64: 11171123.
11. Arden KE,, Mackay IM. 2010. Newly identified human rhinoviruses: molecular methods heat up the cold viruses. Rev Med Virol 20: 156176.
12. Bochkov YA,, Palmenberg AC,, Lee W-M,, Rathe JA,, Amineva SP,, Sun X,, Pasic TR,, Jarjour NN,, Liggett SB,, Gern JE. 2011. Molecular modeling, organ culture and reverse genetics for a newly identified human rhinovirus C. Nat Med 17: 627632.
13. Palmenberg AC,, Spiro D,, Kuzmickas R,, Wang S,, Djikeng A,, Rathe JA,, Fraser-Liggett CM,, Liggett SB. 2009. Sequencing and analyses of all known human rhinovirus genomes reveal structure and evolution. Science 324: 5559.
14. Simmonds P,, McIntyre C,, Savolainen-Kopra C,, Tapparel C,, Mackay IM,, Hovi T. 2010. Proposals for the classification of human rhinovirus species C into genotypically assigned types. J Gen Virol 91: 24092419.
15. McIntyre CL,, Knowles NJ,, Simmonds P. 2013. Proposals for the classification of human rhinovirus species A, B and C into genotypically assigned types. J Gen Virol 94( Pt 8): 17911806.
16. Paul AV,, van Boom JH,, Filippov D,, Wimmer E. 1998. Protein-primed RNA synthesis by purified poliovirus RNA polymerase. Nature 393: 280284.
17. Rossmann MG,, Arnold E,, Erickson JW,, Frankenberger EA,, Griffith JP,, Hecht HJ,, Johnson JE,, Kamer G,, Luo M,, Mosser AG, , et al. 1985. Structure of a human common cold virus and functional relationship to other picornaviruses. Nature 317: 145153.
18. Zhang Y,, Simpson AA,, Ledford RM,, Bator CM,, Chakravarty S,, Skochko GA,, Demenczuk TM,, Watanyar A,, Pevear DC,, Rossmann MG. 2004. Structural and virological studies of the stages of virus replication that are affected by antirhinovirus compounds. J Virol 78: 1106111069.
19. Xing L,, Casasnovas JM,, Cheng RH. 2003. Structural analysis of human rhinovirus complexed with ICAM-1 reveals the dynamics of receptor-mediated virus uncoating. J Virol 77: 61016107.
20. Wisdom A,, Kutkowska AE,, McWilliam Leitch EC,, Gaunt E,, Templeton K,, Harvala H,, Simmonds P. 2009. Genetics, recombination and clinical features of human rhinovirus species C (HRV-C) infections; interactions of HRV-C with other respiratory viruses. PLoS One 4: e8518.
21. Huang T,, Wang W,, Bessaud M,, Ren P,, Sheng J,, Yan H,, Zhang J,, Lin X,, Wang Y,, Delpeyroux F,, Deubel V. 2009. Evidence of recombination and genetic diversity in human rhinoviruses in children with acute respiratory infection. PLoS One 4: e6355.
22. Palmenberg AC,, Rathe JA,, Liggett SB. 2010. Analysis of the complete genome sequences of human rhinovirus. J Allergy Clin Immunol 125: 11901199.
23. McIntyre CL,, Savolainen-Kopra C,, Hovi T,, Simmonds P. 2013. Recombination in the evolution of human rhinovirus genomes. Arch Virol 158: 14971515.
24. Brownlee JW,, Turner RB. 2008. New developments in the epidemiology and clinical spectrum of rhinovirus infections. Curr Opin Pediatr 20: 6771.
25. Linder JE,, Kraft DC,, Mohamed Y,, Lu Z,, Heil L,, Tollefson S,, Saville BR,, Wright PF,, Williams JV,, Miller EK. 2013. Human rhinovirus C: age, season, and lower respiratory illness over the past 3 decades. J Allergy Clin Immunol 131: 6977 e61–66.
26. Piotrowska Z,, Vazquez M,, Shapiro ED,, Weibel C,, Ferguson D,, Landry ML,, Kahn JS. 2009. Rhinoviruses are a major cause of wheezing and hospitalization in children less than 2 years of age. Pediatr Infect Dis J 28: 2529.
27. Nicholson KG,, Kent J,, Hammersley V,, Cancio E. 1996. Risk factors for lower respiratory complications of rhinovirus infections in elderly people living in the community: prospective cohort study. BMJ 313: 11191123.
28. Miller EK,, Edwards KM,, Weinberg GA,, Iwane MK,, Griffin MR,, Hall CB,, Zhu Y,, Szilagyi PG,, Morin LL,, Heil LH,, Lu X,, Williams JV. 2009. A novel group of rhinoviruses is associated with asthma hospitalizations. J Allergy Clin Immunol 123: 98104 e101.
29. Han TH,, Chung JY,, Hwang ES,, Koo JW. 2009. Detection of human rhinovirus C in children with acute lower respiratory tract infections in South Korea. Arch Virol 154: 987991.
30. Onyango CO,, Welch SR,, Munywoki PK,, Agoti CN,, Bett A,, Ngama M,, Myers R,, Cane PA,, Nokes DJ. 2012. Molecular epidemiology of human rhinovirus infections in Kilifi, coastal Kenya. J Med Virol 84: 823831.
31. Dick EC,, Jennings LC,, Mink KA,, Wartgow CD,, Inhorn SL. 1987. Aerosol transmission of rhinovirus colds. J Infect Dis 156: 442448.
32. Douglas RG, Jr. 1970. Pathogenesis of rhinovirus common colds in human voluteers. Ann Otol Rhinol Laryngol 79: 563571.
33. Gwaltney JM,, Moskalski PB,, Hendley JO. 1978. Hand-to-hand transmission of rhinovirus colds. Ann Intern Med 88: 463467.
34. Gwaltney JM, Jr. 1992. Combined antiviral and antimediator treatment of rhinovirus colds. J Infect Dis 166: 776782.
35. Hendley JO. 1999. Clinical virology of rhinoviruses. Adv Virus Res 54: 453466.
36. Turner RB,, Fuls JL,, Rodgers ND. 2010. Effectiveness of hand sanitizers with and without organic acids for removal of rhinovirus from hands. Antimicrob Agents Chemother 54: 13631364.
37. Bochkov YA,, Gern JE. 2012. Clinical and molecular features of human rhinovirus C. Microbes Infect 14: 485494.
38. Hayden FG,, Herrington DT,, Coats TL,, Kim K,, Cooper EC,, Villano SA,, Liu S,, Hudson S,, Pevear DC,, Collett M,, McKinlay M. 2003. Efficacy and safety of oral pleconaril for treatment of colds due to picornaviruses in adults: results of 2 double-blind, randomized, placebo-controlled trials. Clin Infect Dis 36: 15231532.
39. Lu X,, Holloway B,, Dare RK,, Kuypers J,, Yagi S,, Williams JV,, Hall CB,, Erdman DD. 2008. Real-time reverse transcription-PCR assay for comprehensive detection of human rhinoviruses. J Clin Microbiol 46: 533539.
40. Douglas RG,, Cate TR,, Gerone PJ,, Couch RB. 1966. Quantitative rhinovirus shedding patterns in volunteers. Am Rev Respir Dis 94: 159167.
41. Johnston SL,, Sanderson G,, Pattemore PK,, Smith S,, Bardin PG,, Bruce CB,, Lambden PR,, Tyrrell DA,, Holgate ST. 1993. Use of polymerase chain reaction for diagnosis of picornavirus infection in subjects with and without respiratory symptoms. J Clin Microbiol 31: 111117.
42. Wright PF,, Deatly AM,, Karron RA,, Belshe RB,, Shi JR,, Gruber WC,, Zhu Y,, Randolph VB. 2007. Comparison of results of detection of rhinovirus by PCR and viral culture in human nasal wash specimens from subjects with and without clinical symptoms of respiratory illness. J Clin Microbiol 45: 21262129.
43. Xatzipsalti M,, Kyrana S,, Tsolia M,, Psarras S,, Bossios A,, Laza-Stanca V,, Johnston SL,, Papadopoulos NG. 2005. Rhinovirus viremia in children with respiratory infections. Am J Respir Crit Care Med 172: 10371040.
44. Jartti T,, Lehtinen P,, Vuorinen T,, Koskenvuo M,, Ruuskanen O. 2004. Persistence of rhinovirus and enterovirus RNA after acute respiratory illness in children. J Med Virol 72: 695699.
45. Mackay IM,, Lambert SB,, Faux CE,, Arden KE,, Nissen MD,, Sloots TP,, Nolan TM. 2013. Community-wide, contemporaneous circulation of a broad spectrum of human rhinoviruses in healthy Australian preschool-aged children during a 12-month period. J Infect Dis 207: 14331441.
46. Peltola V,, Waris M,, Osterback R,, Susi P,, Hyypia T,, Ruuskanen O. 2008. Clinical effects of rhinovirus infections. J Clin Virol 43: 411414.
47. Kaiser L,, Aubert JD,, Pache JC,, Deffernez C,, Rochat T,, Garbino J,, Wunderli W,, Meylan P,, Yerly S,, Perrin L,, Letovanec I,, Nicod L,, Tapparel C,, Soccal PM. 2006. Chronic rhinoviral infection in lung transplant recipients. Am J Respir Crit Care Med 174: 13921399.
48. Cohen S,, Doyle WJ,, Alper CM,, Janicki-Deverts D,, Turner RB. 2009. Sleep habits and susceptibility to the common cold. Arch Intern Med 169: 6267.
49. Cohen S,, Janicki-Deverts D,, Doyle WJ,, Miller GE,, Frank E,, Rabin BS,, Turner RB. 2012. Chronic stress, glucocorticoid receptor resistance, inflammation, and disease risk. Proc Natl Acad Sci USA 109: 59955999.
50. Cohen S,, Tyrrell DA,, Smith AP. 1991. Psychological stress and susceptibility to the common cold. N Engl J Med 325: 606612.
51. Douglas RG,, Lindgren KM,, Couch RB. 1968. Exposure to cold environment and rhinovirus common cold. N Engl J Med 279: 742747.
52. Barclay WS,, Al-Nakib W. 1987. An ELISA for the detection of rhinovirus specific antibody in serum and nasal secretion. J Virol Methods 15: 5364.
53. Cate TR,, Couch RB,, Johnson KM. 1964. Studies with rhinoviruses in volunteers: production of illness, effect of naturallly acquired antibody, and demonstration of a protective effect not associated with serum antibody. J Clin Invest 43: 5667.
54. Fendrick AM,, Monto AS,, Nightengale B,, Sarnes M. 2003. The economic burden of non-influenza-related viral respiratory tract infection in the United States. Arch Intern Med 163: 487494.
55. Nokso-Koivisto J,, Raty R,, Blomqvist S,, Kleemola M,, Syrjanen R,, Pitkaranta A,, Kilpi T,, Hovi T. 2004. Presence of specific viruses in the middle ear fluids and respiratory secretions of young children with acute otitis media. J Med Virol 72: 241248.
56. Savolainen-Kopra C,, Blomqvist S,, Kilpi T,, Roivainen M,, Hovi T. 2009. Novel species of human rhinoviruses in acute otitis media. Pediatr Infect Dis J 28: 5961.
57. Gern JE. 2009. Rhinovirus and the initiation of asthma. Curr Opin Allergy Clin Immunol 9: 7378.
58. Mallia P,, Johnston SL. 2006. How viral infections cause exacerbation of airway diseases. Chest 130: 12031210.
59. Burns JL,, Emerson J,, Kuypers J,, Campbell AP,, Gibson RL,, McNamara S,, Worrell K,, Englund JA. 2012. Respiratory viruses in children with cystic fibrosis: viral detection and clinical findings. Influenza Other Respir Viruses 6: 218223.
60. Caliskan M,, Bochkov YA,, Kreiner-Moller E,, Bonnelykke K,, Stein MM,, Du G,, Bisgaard H,, Jackson DJ,, Gern JE,, Lemanske RF, Jr,, Nicolae DL,, Ober C. 2013. Rhinovirus wheezing illness and genetic risk of childhood-onset asthma. N Engl J Med 368: 13981407.
61. Longtin J,, Marchand-Austin A,, Winter AL,, Patel S,, Eshaghi A,, Jamieson F,, Low DE,, Gubbay JB. 2010. Rhinovirus outbreaks in long-term care facilities, Ontario, Canada. Emerg Infect Dis 16: 14631465.
62. Hayden FG. 2004. Rhinovirus and the lower respiratory tract. Rev Med Virol 14: 1731.
63. Papadopoulos NG,, Bates PJ,, Bardin PG,, Papi A,, Leir SH,, Fraenkel DJ,, Meyer J,, Lackie PM,, Sanderson G,, Holgate ST,, Johnston SL. 2000. Rhinoviruses infect the lower airways. J Infect Dis 181: 18751884.
64. Lau SKP,, Yip CCY,, Lin AWC,, Lee RA,, So L-Y,, Lau Y-L,, Chan K-H,, Woo PCY,, Yuen K-Y. 2009. Clinical and molecular epidemiology of human rhinovirus C in children and adults in Hong Kong reveals a possible distinct human rhinovirus C subgroup. J Infect Dis 200: 10961103.
65. Xiang Z,, Gonzalez R,, Wang Z,, Xiao Y,, Chen L,, Li T,, Vernet G,, Paranhos-Baccala G,, Jin Q,, Wang J. 2010. Human rhinoviruses in Chinese adults with acute respiratory tract infection. J Infect 61: 289298.
66. Korppi M,, Kotaniemi-Syrjanen A,, Waris M,, Vainionpaa R,, Reijonen TM. 2004. Rhinovirus-associated wheezing in infancy: comparison with respiratory syncytial virus bronchiolitis. Pediatr Infect Dis J 23: 995999.
67. Tsolia MN,, Psarras S,, Bossios A,, Audi H,, Paldanius M,, Gourgiotis D,, Kallergi K,, Kafetzis DA,, Constantopoulos A,, Papadopoulos NG. 2004. Etiology of communityacquired pneumonia in hospitalized school-age children: evidence for high prevalence of viral infections. Clin Infect Dis 39: 681686.
68. Louie JK,, Yagi S,, Nelson FA,, Kiang D,, Glaser CA,, Rosenberg J,, Cahill CK,, Schnurr DP. 2005. Rhinovirus outbreak in a long term care facility for elderly persons associated with unusually high mortality. Clin Infect Dis 41: 262265.
69. Christensen MS,, Nielsen LP,, Hasle H. 2005. Few but severe viral infections in children with cancer: a prospective RT-PCR and PCR-based 12-month study. Pediatr Blood Cancer 45: 945951.
70. Gutman JA,, Peck AJ,, Kuypers J,, Boeckh M. 2007. Rhinovirus as a cause of fatal lower respiratory tract infection in adult stem cell transplantation patients: a report of two cases. Bone Marrow Transplant 40: 809811.
71. Kumar D,, Erdman D,, Keshavjee S,, Peret T,, Tellier R,, Hadjiliadis D,, Johnson G,, Ayers M,, Siegal D,, Humar A. 2005. Clinical impact of community-acquired respiratory viruses on bronchiolitis obliterans after lung transplant. Am J Transplant 5: 20312036.
72. van Kraaij MG,, van Elden LJ,, van Loon AM,, Hendriksen KA,, Laterveer L,, Dekker AW,, Nijhuis M. 2005. Frequent detection of respiratory viruses in adult recipients of stem cell transplants with the use of real-time polymerase chain reaction, compared with viral culture. Clin Infect Dis 40: 662669.
73. Ferguson PE,, Gilroy NM,, Faux CE,, Mackay IM,, Sloots TP,, Nissen MD,, Dwyer DE,, Sorrell TC. 2013. Human rhinovirus C in adult haematopoietic stem cell transplant recipients with respiratory illness. J Clin Virol 56: 255259.
74. Ison MG,, Hayden FG,, Kaiser L,, Corey L,, Boeckh M. 2003. Rhinovirus infections in hematopoietic stem cell transplant recipients with pneumonia. Clin Infect Dis 36: 11391143.
75. Las Heras J,, Swanson VL. 1983. Sudden death of an infant with rhinovirus infection complicating bronchial asthma: case report. Pediatr Pathol 1: 319323.
76. Proud D,, Turner RB,, Winther B,, Wiehler S,, Tiesman JP,, Reichling TD,, Juhlin KD,, Fulmer AW,, Ho BY,, Walanski AA,, Poore CL,, Mizoguchi H,, Jump L,, Moore ML,, Zukowski CK,, Clymer JW. 2008. Gene expression profiles during in vivo human rhinovirus infection: insights into the host response. Am J Respir Crit Care Med 178: 962968.
77. Mallia P,, Footitt J,, Sotero R,, Jepson A,, Contoli M,, Trujillo-Torralbo MB,, Kebadze T,, Aniscenko J,, Oleszkiewicz G,, Gray K,, Message SD,, Ito K,, Barnes PJ,, Adcock IM,, Papi A,, Stanciu LA,, Elkin SL,, Kon OM,, Johnson M,, Johnston SL. 2012. Rhinovirus infection induces degradation of antimicrobial peptides and secondary bacterial infection in chronic obstructive pulmonary disease. Am J Respir Crit Care Med 186: 11171124.
78. Peltola V,, Heikkinen T,, Ruuskanen O,, Jartti T,, Hovi T,, Kilpi T,, Vainionpaa R. 2011. Temporal association between rhinovirus circulation in the community and invasive pneumococcal disease in children. Pediatr Infect Dis J 30: 456461.
79. Khetsuriani N,, Lu X,, Teague WG,, Kazerouni N,, Anderson LJ,, Erdman DD. 2008. Novel human rhinoviruses and exacerbation of asthma in children. Emerg Infect Dis 14: 17931796.
80. Lamson D,, Renwick N,, Kapoor V,, Liu Z,, Palacios G,, Ju J,, Dean A,, St George K,, Briese T,, Lipkin WI. 2006. MassTag polymerase-chain-reaction detection of respiratory pathogens, including a new rhinovirus genotype, that caused influenza-like illness in New York State during 2004–2005. J Infect Dis 194: 13981402.
81. Lee W-M,, Kiesner C,, Pappas T,, Lee I,, Grindle K,, Jartti T,, Jakiela B,, Lemanske RF,, Shult PA,, Gern JE. 2007. A diverse group of previously unrecognized human rhinoviruses are common causes of respiratory illnesses in infants. PLoS One 2: e966.
82. Louie JK,, Roy-Burman A,, Guardia-Labar L,, Boston EJ,, Kiang D,, Padilla T,, Yagi S,, Messenger S,, Petru AM,, Glaser CA,, Schnurr DP. 2009. Rhinovirus associated with severe lower respiratory tract infections in children. Pediatr Infect Dis J 28: 337339.
83. McErlean P,, Shackelton LA,, Lambert SB,, Nissen MD,, Sloots TP,, Mackay IM. 2007. Characterisation of a newly identified human rhinovirus, HRV-QPM, discovered in infants with bronchiolitis. J Clin Virol 39: 6775.
84. Miller EK,, Lu X,, Erdman DD,, Poehling KA,, Zhu Y,, Griffin MR,, Hartert TV,, Anderson LJ,, Weinberg GA,, Hall CB,, Iwane MK,, Edwards KM. 2007. Rhinovirus-associated hospitalizations in young children. J Infect Dis 195: 773781.
85. Ashraf S,, Brockman-Schneider R,, Bochkov YA,, Pasic TR,, Gern JE. 2013. Biological characteristics and propagation of human rhinovirus-C in differentiated sinus epithelial cells. Virology 436: 143149.
86. Lau SK,, Yip CC,, Lung DC,, Lee P,, Que TL,, Lau YL,, Chan KH,, Woo PC,, Yuen KY. 2012. Detection of human rhinovirus C in fecal samples of children with gastroenteritis. J Clin Virol 53: 290296.
87. Tapparel C,, Cordey S,, Van Belle S,, Turin L,, Lee W-M,, Regamey N,, Meylan P,, Muhlemann K,, Gobbini F,, Kaiser L. 2009. New molecular detection tools adapted to emerging rhinoviruses and enteroviruses. J Clin Microbiol 47: 17421749.
88. Harvala H,, McIntyre CL,, McLeish NJ,, Kondracka J,, Palmer J,, Molyneaux P,, Gunson R,, Bennett S,, Templeton K,, Simmonds P. 2012. High detection frequency and viral loads of human rhinovirus species A to C in fecal samples; diagnostic and clinical implications. J Med Virol 84: 536542.
89. Miller EK,, Mackay IM. 2013. From sneeze to wheeze: what we know about rhinovirus Cs. J Clin Virol 57: 291299.
90. Kiang D,, Kalra I,, Yagi S,, Louie JK,, Boushey H,, Boothby J,, Schnurr DP. 2008. Assay for 5′ noncoding region analysis of all human rhinovirus prototype strains. J Clin Microbiol 46: 37363745.
91. Arruda E,, Crump CE,, Rollins BS,, Ohlin A,, Hayden FG. 1996. Comparative susceptibilities of human embryonic fibroblasts and HeLa cells for isolation of human rhinoviruses. J Clin Microbiol 34: 12771279.
92. Spyridaki IS,, Christodoulou I,, de Beer L,, Hovland V,, Kurowski M,, Olszewska-Ziaber A,, Carlsen K-H,, Lodrup-Carlsen K,, van Drunen CM,, Kowalski ML,, Molenkamp R,, Papadopoulos NG. 2009. Comparison of four nasal sampling methods for the detection of viral pathogens by RT-PCR-A GA(2)LEN project. J Virol Methods 156: 102106.
93. Waris M,, Osterback R,, Lahti E,, Vuorinen T,, Ruuskanen O,, Peltola V. 2013. Comparison of sampling methods for the detection of human rhinovirus RNA. J Clin Virol 58: 200204.
94. Debyle C,, Bulkow L,, Miernyk K,, Chikoyak L,, Hummel KB,, Hennessy T,, Singleton R. 2012. Comparison of nasopharyngeal flocked swabs and nasopharyngeal wash collection methods for respiratory virus detection in hospitalized children using real-time polymerase chain reaction. J Virol Methods 185: 8993.
95. Larios OE,, Coleman BL,, Drews SJ,, Mazzulli T,, Borgundvaag B,, Green K,, Group ST-FS,, McGeer AJ. 2011. Self-collected mid-turbinate swabs for the detection of respiratory viruses in adults with acute respiratory illnesses. PLoS One 6: e21335.
96. Esposito S,, Molteni CG,, Daleno C,, Valzano A,, Tagliabue C,, Galeone C,, Milani G,, Fossali E,, Marchisio P,, Principi N. 2010. Collection by trained pediatricians or parents of mid-turbinate nasal flocked swabs for the detection of influenza viruses in childhood. Virol J. 7: 85.
97. Xiang X,, Qiu D,, Chan KP,, Chan SH,, Hegele RG,, Tan WC. 2002. Comparison of three methods for respiratory virus detection between induced sputum and nasopharyngeal aspirate specimens in acute asthma. J Virol Methods 101: 127133.
98. Falsey AR,, Formica MA,, Walsh EE. 2012. Yield of sputum for viral detection by reverse transcriptase PCR in adults hospitalized with respiratory illness. J Clin Microbiol 50: 2124.
99. Freymuth F,, Vabret A,, Cuvillon-Nimal D,, Simon S,, Dina J,, Legrand L,, Gouarin S,, Petitjean J,, Eckart P,, Brouard J. 2006. Comparison of multiplex PCR assays and conventional techniques for the diagnostic of respiratory virus infections in children admitted to hospital with an acute respiratory illness. J Med Virol 78: 14981504.
100. Hyypia T,, Puhakka T,, Ruuskanen O,, Makela M,, Arola A,, Arstila P. 1998. Molecular diagnosis of human rhinovirus infections: comparison with virus isolation. J Clin Microbiol 36: 20812083.
101. Steininger C,, Aberle SW,, Popow-Kraupp T. 2001. Early detection of acute rhinovirus infections by a rapid reverse transcription-PCR assay. J Clin Microbiol 39: 129133.
102. Vuorinen T,, Vainionpaa R,, Hyypia T. 2003. Five years’ experience of reverse-transcriptase polymerase chain reaction in daily diagnosis of enterovirus and rhinovirus infections. Clin Infect Dis 37: 452455.
103. Blomqvist S,, Skytta A,, Roivainen M,, Hovi T. 1999. Rapid detection of human rhinoviruses in nasopharyngeal aspirates by a microwell reverse transcription-PCR-hybridization assay. J Clin Microbiol 37: 28132816.
104. Gama RE,, Hughes PJ,, Bruce CB,, Stanway G. 1988. Polymerase chain reaction amplification of rhinovirus nucleic acids from clinical material. Nucleic Acids Res 16: 93469346.
105. Olive DM,, Al-Mufti S,, Al-Mulla W,, Khan MA,, Pasca A,, Stanway G,, Al-Nakib W. 1990. Detection and differentiation of picornaviruses in clinical samples following genomic amplification. J Gen Virol 71( Pt 9): 21412147.
106. Atmar RL,, Georghiou PR. 1993. Classification of respiratory tract picornavirus isolates as enteroviruses or rhinoviruses by using reverse transcription-polymerase chain reaction. J Clin Microbiol 31: 25442546.
107. Papadopoulos NG,, Hunter J,, Sanderson G,, Meyer J,, Johnston SL. 1999. Rhinovirus identification by BglI digestion of picornavirus RT-PCR amplicons. J Virol Methods 80: 179185.
108. Halonen P,, Rocha E,, Hierholzer J,, Holloway B,, Hyypia T,, Hurskainen P,, Pallansch M. 1995. Detection of enteroviruses and rhinoviruses in clinical specimens by PCR and liquid-phase hybridization. J Clin Microbiol 33: 648653.
109. Deffernez C,, Wunderli W,, Thomas Y,, Yerly S,, Perrin L,, Kaiser L. 2004. Amplicon sequencing and improved detection of human rhinovirus in respiratory samples. J Clin Microbiol 42: 32123218.
110. Faux CE,, Arden KE,, Lambert SB,, Nissen MD,, Nolan TM,, Chang AB,, Sloots TP,, Mackay IM. 2011. Usefulness of published PCR primers in detecting human rhinovirus infection. Emerg Infect Dis 17: 296298.
111. Dagher H,, Donninger H,, Hutchinson P,, Ghildyal R,, Bardin P. 2004. Rhinovirus detection: comparison of real-time and conventional PCR. J Virol Methods 117: 113121.
112. Kares S,, Lonnrot M,, Vuorinen P,, Oikarinen S,, Taurianen S,, Hyoty H. 2004. Real-time PCR for rapid diagnosis of entero- and rhinovirus infections using LightCycler. J Clin Virol 29: 999104.
113. Templeton KE,, Forde CB,, Loon AMv,, Claas ECJ,, Niesters HGM,, Wallace P,, Carman WF. 2006. A multi-centre pilot proficiency programme to assess the quality of molecular detection of respiratory viruses. J Clin Virol 35: 5158.
114. Iwane MK,, Prill MM,, Lu X,, Miller EK,, Edwards KM,, Hall CB,, Griffin MR,, Staat MA,, Anderson LJ,, Williams JV,, Weinberg GA,, Ali A,, Szilagyi PG,, Zhu Y,, Erdman DD. 2011. Human rhinovirus species associated with hospitalizations for acute respiratory illness in young US children. J Infect Dis 204: 17021710.
115. Soto-Quiros M,, Avila L,, Platts-Mills TA,, Hunt JF,, Erdman DD,, Carper H,, Murphy DD,, Odio S,, James HR,, Patrie JT,, Hunt W,, O’Rourke AK,, Davis MD,, Steinke JW,, Lu X,, Kennedy J,, Heymann PW. 2012. High titers of IgE antibody to dust mite allergen and risk for wheezing among asthmatic children infected with rhinovirus. J Allergy Clin Immunol 129: 14991505 e1495.
116. Takeyama A,, Hashimoto K,, Sato M,, Sato T,, Kanno S,, Takano K,, Ito M,, Katayose M,, Nishimura H,, Kawasaki Y,, Hosoya M. 2012. Rhinovirus load and disease severity in children with lower respiratory tract infections. J Med Virol 84: 11351142.
117. Utokaparch S,, Marchant D,, Gosselink JV,, McDonough JE,, Thomas EE,, Hogg JC,, Hegele RG. 2011. The relationship between respiratory viral loads and diagnosis in children presenting to a pediatric hospital emergency department. Pediatr Infect Dis J 30: e1823.
118. Gerna G,, Piralla A,, Rovida F,, Rognoni V,, Marchi A,, Locatelli F,, Meloni F. 2009. Correlation of rhinovirus load in the respiratory tract and clinical symptoms in hospitalized immunocompetent and immunocompromised patients. J Med Virol 81: 14981507.
119. Franz A,, Adams O,, Willems R,, Bonzel L,, Neuhausen N,, Schweizer-Krantz S,, Ruggeberg JU,, Willers R,, Henrich B,, Schroten H,, Tenenbaum T. 2010. Correlation of viral load of respiratory pathogens and co-infections with disease severity in children hospitalized for lower respiratory tract infection. J Clin Virol 48: 239245.
120. Schibler M,, Yerly S,, Vieille G,, Docquier M,, Turin L,, Kaiser L,, Tapparel C. 2012. Critical analysis of rhinovirus RNA load quantification by real-time reverse transcription-PCR. J Clin Microbiol 50: 28682872.
121. Chandrasekaran A,, Manji R,, Joseph A,, Zhang F,, Ginocchio CC. 2012. Broad reactivity of the Luminex xTAG Respiratory Virus Panel (RVP) assay for the detection of human rhinoviruses. J Clin Virol 53: 272273.
122. Merante F,, Yaghoubian S,, Janeczko R. 2007. Principles of the xTAG respiratory viral panel assay (RVP Assay). J Clin Virol 40( Suppl 1): S3135.
123. Pierce VM,, Hodinka RL. 2012. Comparison of the GenMark Diagnostics eSensor respiratory viral panel to real-time PCR for detection of respiratory viruses in children. J Clin Microbiol 50: 34583465.
124. Popowitch EB,, O’Neill SS,, Miller MB. 2013. Comparison of the Biofire FilmArray RP, Genmark eSensor RVP, Luminex xTAG RVPv1, and Luminex xTAG RVP fast multiplex assays for detection of respiratory viruses. J Clin Microbiol 51: 15281533.
125. Sakthivel SK,, Whitaker B,, Lu X,, Oliveira DBL,, Stockman LJ,, Kamili S,, Oberste MS,, Erdman DD. 2012. Comparison of fast-track diagnostics respiratory pathogens multiplex real-time RT-PCR assay with in-house singleplex assays for comprehensive detection of human respiratory viruses. J Virol Methods 185: 259266.
126. Jaramillo-Gutierrez G,, Benschop KSM,, Claas ECJ,, de Jong AS,, van Loon AM,, Pas SD,, Pontesilli O,, Rossen JW,, Swanink CMA,, Thijsen S,, van der Zanden AGM,, van der Avoort HGAM,, Koopmans MPG,, Meijer A. 2013. September through October 2010 multi-centre study in the Netherlands examining laboratory ability to detect enterovirus 68, an emerging respiratory pathogen. J Virol Methods 190: 5362.
127. Piralla A,, Lilleri D,, Sarasini A,, Marchi A,, Zecca M,, Stronati M,, Baldanti F,, Gerna G. 2012. Human rhinovirus and human respiratory enterovirus (EV68 and EV104) infections in hospitalized patients in Italy, 2008–2009. Diagn Microbiol Infect Dis 73: 162167.
128. Xiang Z,, Gonzalez R,, Wang Z,, Ren L,, Xiao Y,, Li J,, Li Y,, Vernet G,, Paranhos-Baccala G,, Jin Q,, Wang J. 2012. Coxsackievirus A21, enterovirus 68, and acute respiratory tract infection, China. Emerg Infect Dis 18: 821824.
129. Jacobson LM,, Redd JT,, Schneider E,, Lu X,, Chern S-WW,, Oberste MS,, Erdman DD,, Fischer GE,, Armstrong GL,, Kodani M,, Montoya J,, Magri JM,, Cheek JE. 2012. Outbreak of lower respiratory tract illness associated with human enterovirus 68 among American Indian children. Pediatr Infect Dis J 31: 309312.
130. Lauinger IL,, Bible JM,, Halligan EP,, Aarons EJ,, MacMahon E,, Tong CY. 2012. Lineages, sub-lineages and variants of enterovirus 68 in recent outbreaks. PLoS One 7: e36005.
131. Geist FC,, Hayden FG. 1985. Comparative susceptibilities of strain MRC-5 human embryonic lung fibroblast cells and the Cooney strain of human fetal tonsil cells for isolation of rhinoviruses from clinical specimens. J Clin Microbiol 22: 455456.
132. Jang YJ,, Lee SH,, Kwon HJ,, Chung YS,, Lee BJ. 2005. Development of rhinovirus study model using organ culture of turbinate mucosa. J Virol Methods 125: 4147.
133. Wang JH,, Kwon HJ,, Chung Y-S,, Lee B-J,, Jang YJ. 2008. Infection rate and virus-induced cytokine secretion in experimental rhinovirus infection in mucosal organ culture: comparison between specimens from patients with chronic rhinosinusitis with nasal polyps and those from normal subjects. Arch Otolaryngol Head Neck Surg 134: 424427.
134. Leonardi GP,, Desormeaux ML. 2010. A rapid microscopic method for the confirmation of rhinoviruses in cell culture. Intervirology 53: 426428.
135. Torgersen H,, Skern T,, Blaas D. 1989. Typing of human rhinoviruses based on sequence variations in the 5′ non-coding region. J Gen Virol 70( Pt 11): 31113116.
136. Couch RB, Atmar RL. 1999. Rhinoviruses. Marcel Dekker, Inc., New York, NY.
137. Lu X, Erdman DD. 2009. Comparison of three typing methods form human rhinoviruses based on sequence analysis of partial 5′NCR, VP4/VP2 and VP1 regions. 25th Annual Clinical Virology Symposium, Daytona Beach, FL.
138. Douglas RG, Jr.,, Fleet WF,, Cate TR,, Couch RB. 1968. Antibody to rhinovirus in human sera. I. Standardization of a neutralization test. Proc Soc Exp Biol Med 127: 497502.
139. De Palma AM,, Vliegen I,, De Clercq E,, Neyts J. 2008. Selective inhibitors of picornavirus replication. Med Res Rev 28: 823884.
140. Clejan S,, Mandrea E,, Pandrea IV,, Dufour J,, Japa S,, Veazey RS. 2005. Immune responses induced by intranasal imiquimod and implications for therapeutics in rhinovirus infections. J Cell Mol Med 9: 457461.
141. Hayden FG,, Diamond L,, Wood PB,, Korts DC,, Wecker MT. 1996. Effectiveness and safety of intranasal ipratropium bromide in common colds. A randomized, double-blind, placebo-controlled trial. Ann Intern Med 125: 8997.
142. Turner RB,, Wecker MT,, Pohl G,, Witek TJ,, McNally E,, St George R,, Winther B,, Hayden FG. 1999. Efficacy of tremacamra, a soluble intercellular adhesion molecule 1, for experimental rhinovirus infection: a randomized clinical trial. JAMA. 281: 17971804.
143. Turner RB,, Bauer R,, Woelkart K,, Hulsey TC,, Gangemi JD. 2005. An evaluation of Echinacea angustifolia in experimental rhinovirus infections. N Engl J Med 353: 341348.
144. Jansen RR,, Wieringa J,, Koekkoek SM,, Visser CE,, Pajkrt D,, Molenkamp R,, de Jong MD,, Schinkel J. 2011. Frequent detection of respiratory viruses without symptoms: toward defining clinically relevant cutoff values. J Clin Microbiol 49: 26312636.
145. Raymond F,, Carbonneau J,, Boucher N,, Robitaille L,, Boisvert S,, Wu W-K,, De Serres G,, Boivin G,, Corbeil J. 2009. Comparison of automated microarray detection with real-time PCR assays for detection of respiratory viruses in specimens obtained from children. J Clin Microbiol 47: 743750.
146. Hayden RT,, Gu Z,, Rodriguez A,, Tanioka L,, Ying C,, Morgenstern M,, Bankowski MJ. 2012. Comparison of two broadly multiplexed PCR systems for viral detection in clinical respiratory tract specimens from immunocompromised children. J Clin Virol 53: 308313.
147. Balada-Llasat J-M,, LaRue H,, Kelly C,, Rigali L,, Pancholi P. 2011. Evaluation of commercial ResPlex II v2.0, MultiCode-PLx, and xTAG respiratory viral panels for the diagnosis of respiratory viral infections in adults. J Clin Virol 50: 4245.
148. Babady NE,, Mead P,, Stiles J,, Brennan C,, Li H,, Shuptar S,, Stratton CW,, Tang Y-W,, Kamboj M. 2012. Comparison of the Luminex xTAG RVP Fast assay and the Idaho Technology FilmArray RP assay for detection of respiratory viruses in pediatric patients at a cancer hospital. J Clin Microbiol 50: 22822288.
149. Kim H-K,, Oh S-H,, Yun KA,, Sung H,, Kim M-N. 2013. Comparison of Anyplex II RV16 with the xTAG respiratory viral panel and Seeplex RV15 for detection of respiratory viruses. J Clin Microbiol 51: 11371141.
150. Rand KH,, Rampersaud H,, Houck HJ. 2011. Comparison of two multiplex methods for detection of respiratory viruses: FilmArray RP and xTAG RVP. J Clin Microbiol 49: 24492453.
151. Loens K,, van Loon AM,, Coenjaerts F,, van Aarle Y,, Goossens H,, Wallace P,, Claas EJC,, Ieven M. 2012. Performance of different mono- and multiplex nucleic acid amplification tests on a multipathogen external quality assessment panel. J Clin Microbiol 50: 977987.
152. Dabisch-Ruthe M,, Vollmer T,, Adams O,, Knabbe C,, Dreier J. 2012. Comparison of three multiplex PCR assays for the detection of respiratory viral infections: evaluation of xTAG respiratory virus panel fast assay, RespiFinder 19 assay and RespiFinder SMART 22 assay. BMC Infect Dis 12: 163163.
153. Brown PK,, Tyrrell DA. 1964. Experiments on the sensitivity of strains of human fibroblasts to infection with rhinoviruses. Br J Exp Pathol 45: 571578.
154. Turner RB,, Fuls JL,, Rodgers ND,, Goldfarb HB,, Lockhart LK,, Aust LB. 2012. A randomized trial of the efficacy of hand disinfection for prevention of rhinovirus infection. Clin Infect Dis 54: 14221426.


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Commercial multiplex molecular assays for respiratory pathogens, including HRV

Citation: Landry M, Lu X. 2015. Rhinoviruses, p 1551-1564. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch89
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Comparison of diagnostic methods for rhinoviruses

Citation: Landry M, Lu X. 2015. Rhinoviruses, p 1551-1564. In Jorgensen J, Pfaller M, Carroll K, Funke G, Landry M, Richter S, Warnock D (ed), Manual of Clinical Microbiology, Eleventh Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817381.ch89

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