Chapter 19 : Antibody Engineering

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Monoclonal antibodies are enjoying outstanding commercial success, with 4 of the top 10 best-selling drugs. Although most of the preclinical and clinical experiences with therapeutic antibodies have been gained from the treatment of cancer and inflammatory diseases, effective therapy is not limited to these indications. For example, palivizumab (Synagis) has been approved for the prophylactic treatment for respiratory syncytial virus (RSV) infections and remains the only therapeutic antibody for the treatment of infectious diseases to date.

Citation: Lo K, Leger O, Hock B. 2015. Antibody Engineering, p 321-343. In Crowe J, Boraschi D, Rappuoli R (ed), Antibodies for Infectious Diseases. ASM Press, Washington, DC. doi: 10.1128/microbiolspec.AID-0007-2012
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Figure 1

Flow chart describing the main decision-making points and their evolution over the years, in a typical CDR-grafting protocol. CDR, complementarity-determining region; HV, hypervariable loops; SDR, specificity-determining regions; MSD, minimum specificity determinant. REI, NEW, and KOL are light or heavy variable region sequences derived from human myeloma cell lines.

Citation: Lo K, Leger O, Hock B. 2015. Antibody Engineering, p 321-343. In Crowe J, Boraschi D, Rappuoli R (ed), Antibodies for Infectious Diseases. ASM Press, Washington, DC. doi: 10.1128/microbiolspec.AID-0007-2012
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