Chapter 8 : Use of Human Hybridoma Technology To Isolate Human Monoclonal Antibodies

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Monoclonal antibodies (mAbs) have revolutionized the conduct of science since their first description in 1975 ( ). The use of these specific reagents also has made possible improved clinical diagnostics in the medical arena, and many antibodies have found their way to clinical use as prophylactic or therapeutic agents. Nevertheless, the potential of mAbs derived specifically from technology based on human hybridomas remains largely unfulfilled. The principal reason for the lack of a large number of hybridoma-derived mAb therapeutics has simply been the technical difficulty in generating stable hybridomas that secrete human mAbs of high affinity and functional activity. This chapter reviews recent efforts to develop and employ novel methods for the efficient generation of human hybridomas secreting human mAbs for clinical use.

Citation: Smith S, Crowe J. 2015. Use of Human Hybridoma Technology To Isolate Human Monoclonal Antibodies, p 141-156. In Crowe J, Boraschi D, Rappuoli R (ed), Antibodies for Infectious Diseases. ASM Press, Washington, DC. doi: 10.1128/microbiolspec.AID-0027-2014
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Figure 1

Lymphoblastoid cell formation from PBMCs.

Citation: Smith S, Crowe J. 2015. Use of Human Hybridoma Technology To Isolate Human Monoclonal Antibodies, p 141-156. In Crowe J, Boraschi D, Rappuoli R (ed), Antibodies for Infectious Diseases. ASM Press, Washington, DC. doi: 10.1128/microbiolspec.AID-0027-2014
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Figure 2

(A) Pre- and (B) post-pearl chain formation.

Citation: Smith S, Crowe J. 2015. Use of Human Hybridoma Technology To Isolate Human Monoclonal Antibodies, p 141-156. In Crowe J, Boraschi D, Rappuoli R (ed), Antibodies for Infectious Diseases. ASM Press, Washington, DC. doi: 10.1128/microbiolspec.AID-0027-2014
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Figure 3

Cloning in (A) semi-solid medium and (B) final human hybridoma.

Citation: Smith S, Crowe J. 2015. Use of Human Hybridoma Technology To Isolate Human Monoclonal Antibodies, p 141-156. In Crowe J, Boraschi D, Rappuoli R (ed), Antibodies for Infectious Diseases. ASM Press, Washington, DC. doi: 10.1128/microbiolspec.AID-0027-2014
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