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Category: Clinical Microbiology
Direct Detection of Viruses and Chlamydia in Clinical Samples, Page 1 of 2
< Previous page | Next page > /docserver/preview/fulltext/10.1128/9781555817435/9781555815271_Chap10_7-1.gif /docserver/preview/fulltext/10.1128/9781555817435/9781555815271_Chap10_7-2.gifAbstract:
Commercially available assays for diagnostic testing are available for several viral agents and for Chlamydia trachomatis ( Table 10.7-1 ) and are discussed in section 12.
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Preparation of aspirates and fluids for IF.
Preparation of aspirates and fluids for IF.
Preparation of swabs, scrapings, and impression smears for IF.
Preparation of swabs, scrapings, and impression smears for IF.
Leukocyte preparation with several cells showing intense CMV pp65 staining and a few cells (arrows) showing weak speckled nuclear staining (×400). Provided by Chemicon International.
Leukocyte preparation with several cells showing intense CMV pp65 staining and a few cells (arrows) showing weak speckled nuclear staining (×400). Provided by Chemicon International.
(A) Kidney tubule with characteristic “owl eye” inclusion of CMV (arrow). Courtesy of W. L. Drew. (B) Giemsa stain of conjunctival cells showing cytoplasmic inclusion (arrow) of C. trachomatis. Courtesy of W. L. Drew. (C) Cytologic examination (Tzanck smear) of scrapings from the base of an ulcerative HSV lesion showing multinucleated giant cells. Courtesy of W. L. Drew. (D) Large eosinophilic inclusions fill the cytoplasms of several infected basal cells (molluscum bodies). Courtesy of W. L. Drew.
(A) Kidney tubule with characteristic “owl eye” inclusion of CMV (arrow). Courtesy of W. L. Drew. (B) Giemsa stain of conjunctival cells showing cytoplasmic inclusion (arrow) of C. trachomatis. Courtesy of W. L. Drew. (C) Cytologic examination (Tzanck smear) of scrapings from the base of an ulcerative HSV lesion showing multinucleated giant cells. Courtesy of W. L. Drew. (D) Large eosinophilic inclusions fill the cytoplasms of several infected basal cells (molluscum bodies). Courtesy of W. L. Drew.
Direct specimen testing: viral and chlamydial infections a
a Abbreviations: OIA, optic immunoassay; HBV, hepatitis B virus; HIV-1, human immunodeficiencyvirus type 1; HPV, human papillomavirus.
b Refer to kit manufacturer regarding specimens for which the reagent can be used and specific instructionsregarding collection, transport, and storage of specimens.
c Anti-chlamydial LPS MAbs may cross-react with bacteria present in vaginal, rectal, and oral specimens, and false-positive results have been reported with EIA testing of vaginal specimens from children ( 2 , 5 , 6 , 11 ) and of rectal specimens from adults ( 12 ) and children ( 11 ) and with direct IF staining of rectal smears from adults and children ( 6 , 11 ).
Direct specimen testing: viral and chlamydial infections a
a Abbreviations: OIA, optic immunoassay; HBV, hepatitis B virus; HIV-1, human immunodeficiencyvirus type 1; HPV, human papillomavirus.
b Refer to kit manufacturer regarding specimens for which the reagent can be used and specific instructionsregarding collection, transport, and storage of specimens.
c Anti-chlamydial LPS MAbs may cross-react with bacteria present in vaginal, rectal, and oral specimens, and false-positive results have been reported with EIA testing of vaginal specimens from children ( 2 , 5 , 6 , 11 ) and of rectal specimens from adults ( 12 ) and children ( 11 ) and with direct IF staining of rectal smears from adults and children ( 6 , 11 ).
Troubleshooting IF problems
Troubleshooting IF problems
Troubleshooting IF problems
Troubleshooting IF problems
Inclusion morphology
a Abbreviations: VZV, varicella-zoster virus; HPV, human papillomavirus.
Inclusion morphology
a Abbreviations: VZV, varicella-zoster virus; HPV, human papillomavirus.