Chapter 11.10 : Total Viable Cell Counting Procedure

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Trypan blue allows rapid observation of nonviable cells present in a peripheral blood mononuclear cell suspension (PBMCS). A count of viable cells present in a PBMCS allows the suspension to be adjusted accordingly for further use in assays.

Citation: Garcia L. 2010. Total Viable Cell Counting Procedure, p 210-211. In Clinical Microbiology Procedures Handbook, 3rd Edition. ASM Press, Washington, DC. doi: 10.1128/9781555817435.ch11.10
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1. Kruse, P. F.,, and M. K. Patterson. 1973. Tissue Culture—Methods and Applications, p. 406. Academic Press, New York, NY.

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